##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162764
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.949743186454008	33.0	31.0	34.0	30.0	34.0
2	32.142230468654	33.0	31.0	34.0	30.0	34.0
3	32.260063650438674	33.0	31.0	34.0	30.0	34.0
4	35.87899044014647	37.0	35.0	37.0	35.0	37.0
5	35.91026271165614	37.0	35.0	37.0	35.0	37.0
6	35.866887026615224	37.0	35.0	37.0	35.0	37.0
7	36.000651249662084	37.0	35.0	37.0	35.0	37.0
8	35.81266742031407	37.0	35.0	37.0	35.0	37.0
9	37.63975449116512	39.0	37.0	39.0	35.0	39.0
10	36.921462977071094	39.0	37.0	39.0	32.0	39.0
11	36.93883782654641	39.0	37.0	39.0	33.0	39.0
12	37.11735396033521	39.0	37.0	39.0	34.0	39.0
13	37.0839804870856	39.0	37.0	39.0	33.0	39.0
14	38.14380944189133	40.0	37.0	41.0	33.0	41.0
15	38.161196579096114	40.0	37.0	41.0	33.0	41.0
16	38.25460175468777	40.0	38.0	41.0	33.0	41.0
17	38.25798088029294	40.0	38.0	41.0	33.0	41.0
18	37.976315401440125	39.0	37.0	40.0	34.0	41.0
19	37.61580570642157	39.0	37.0	40.0	34.0	41.0
20	37.09922955936202	38.0	35.0	40.0	33.0	41.0
21	36.97387628713966	38.0	35.0	40.0	33.0	41.0
22	37.01859133469318	38.0	35.0	40.0	33.0	41.0
23	36.961275220564744	38.0	35.0	40.0	33.0	41.0
24	36.84500872428793	38.0	35.0	40.0	33.0	41.0
25	36.76390356589909	38.0	35.0	40.0	33.0	41.0
26	36.7240667469465	38.0	35.0	40.0	33.0	41.0
27	36.64920989899486	38.0	35.0	40.0	32.0	41.0
28	36.53742842397582	38.0	35.0	40.0	32.0	41.0
29	36.463327271386795	38.0	35.0	40.0	32.0	41.0
30	36.2866051461011	38.0	35.0	40.0	31.0	41.0
31	35.90413113464894	38.0	35.0	40.0	30.0	41.0
32	35.35723501511391	38.0	35.0	40.0	27.0	41.0
33	34.77770268609766	38.0	34.0	40.0	22.0	41.0
34	34.2675407338232	38.0	34.0	40.0	18.0	41.0
35	33.88272590990637	38.0	34.0	40.0	15.0	41.0
36	33.62234892236612	38.0	33.0	40.0	12.0	41.0
37	33.42350274016367	38.0	33.0	40.0	10.0	41.0
38	33.3052333439827	38.0	33.0	40.0	10.0	41.0
39	33.270882996239955	38.0	33.0	40.0	10.0	41.0
40	33.19048438229584	38.0	33.0	40.0	10.0	41.0
41	33.06286402398565	38.0	33.0	40.0	10.0	41.0
42	32.95454154481335	38.0	32.0	40.0	10.0	41.0
43	32.77041606251997	38.0	32.0	40.0	10.0	41.0
44	32.634655083433685	38.0	31.0	40.0	10.0	41.0
45	32.61509301811211	38.0	31.0	40.0	10.0	41.0
46	32.33949767761913	37.0	31.0	40.0	9.0	41.0
47	32.11057113366592	37.0	31.0	40.0	8.0	41.0
48	32.04797129586395	37.0	30.0	40.0	8.0	41.0
49	32.12724558256125	37.0	31.0	40.0	8.0	41.0
50	32.038878376053674	37.0	31.0	40.0	8.0	41.0
51	31.097410975399967	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	0.0
13	4.0
14	8.0
15	3.0
16	13.0
17	41.0
18	47.0
19	129.0
20	203.0
21	336.0
22	623.0
23	1074.0
24	1888.0
25	3690.0
26	5522.0
27	5576.0
28	4608.0
29	3996.0
30	4021.0
31	4232.0
32	5025.0
33	6315.0
34	8890.0
35	11852.0
36	15710.0
37	21095.0
38	34101.0
39	23751.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.24715539062692	4.669337199872207	43.829102258484674	28.254405151016194
2	38.64920989899486	5.249318031014229	39.91607480769703	16.185397262293876
3	16.793639871224595	5.48278489100784	60.17915509572141	17.544420142046153
4	13.302081541372784	4.557518861664741	61.74645499004694	20.393944606915536
5	12.843134845543242	5.871077142365634	61.51667444889534	19.76911356319579
6	15.982649725983633	8.01159961662284	64.39015998623773	11.615590671155784
7	64.20277211176919	1.7251972180580473	30.10125089086039	3.9707797793123785
8	63.85072866235777	2.688555208768524	27.812661276449337	5.6480548524243686
9	58.39251923029663	4.71111548008159	30.410287287115086	6.486078002506697
10	26.739942493426067	19.943599321717333	37.33626600476764	15.980192180088961
11	17.584969649308203	19.507384925413483	42.2194096974761	20.68823572780222
12	15.411884691946623	16.380157774446438	47.992185004054946	20.21577252955199
13	19.751296355459438	17.705389398147013	49.63321127522056	12.910102971172988
14	18.078936374136788	19.21739450984247	47.67577597011624	15.0278931459045
15	12.245951193138533	19.664053476198667	50.03624880194638	18.053746528716424
16	11.786390110835319	18.598707330859405	47.51972180580472	22.095180752500553
17	12.040131724459954	18.89852793000909	45.532181563490695	23.529158782040255
18	13.251701850532058	17.15797105010936	49.30820083065051	20.28212626870807
19	14.190484382295839	20.631097785751148	48.9051633039247	16.27325452802831
20	14.174510333980486	23.493524366567545	46.26391585362857	16.0680494458234
21	12.367599714924676	25.361259246516425	46.57540979577794	15.695731242780958
22	10.953896439015999	22.65734437590622	44.65299451967265	21.735764665405128
23	10.921333955911626	23.473863999410188	42.25995920473815	23.344842839940036
24	11.755056400678281	22.817084859059744	43.07525005529478	22.352608684967194
25	10.649775134550637	25.17940085031088	40.9248973974589	23.245926617679586
26	10.412621955715023	25.316409033938708	44.2978791378929	19.973089872453368
27	12.380501830871692	24.065518173551894	44.35010198815463	19.20387800742179
28	9.839399375783344	21.346858027573663	45.1420461527119	23.67169644393109
29	11.843528052886388	19.742080558354427	44.81887886756285	23.595512521196333
30	16.58474847017768	21.863557051927945	41.015212209088006	20.53648226880637
31	14.186798063453834	27.040377479049422	35.286672728613205	23.486151728883538
32	12.118773193089382	27.317465778673416	35.2737706126662	25.289990415571012
33	15.045710353640853	22.51787864638372	34.86458922120371	27.57182177877172
34	13.94472979282888	20.349094394337815	35.68479516354968	30.021380649283625
35	12.335651618293971	20.70420977611757	37.32029195645229	29.63984664913617
36	12.591236391339608	20.776092993536654	37.61888378265464	29.013786832469098
37	12.00572608193458	23.210906588680544	32.774446437787226	32.008920891597654
38	12.965397753803051	26.657000319480968	31.676537809343593	28.701064117372393
39	14.984886092747782	23.406281487306778	37.515666855078514	24.093165564866926
40	15.338158315106535	19.304023002629574	37.31230493229461	28.04551374996928
41	12.958025116119043	19.598314123516257	33.63827381976358	33.80538694060112
42	13.929370130987195	19.48158069351945	37.98690128038141	28.60214789511194
43	13.961932614091568	20.24157676144602	40.7043326534123	25.09215797105011
44	14.586149271337643	21.38494998894104	35.0267872502519	29.002113489469416
45	17.992922267823353	19.421985205573712	33.86068172323118	28.724410803371754
46	18.582118896070384	19.808434297510505	36.12408149222187	25.485365314197246
47	12.491705782605491	23.122434936472438	38.60743161878548	25.77842766213659
48	12.562974613550908	22.264137032759088	40.02482121353616	25.14806714015384
49	18.027942296822395	18.03285738861173	40.36027622815856	23.578924086407312
50	16.7408026344892	16.47845961023322	36.83738418814971	29.94335356712787
51	15.329556904475192	16.392445503919788	33.328623037035214	34.94937455456981
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	369.5
2	547.0
3	5234.0
4	9921.0
5	6383.0
6	2845.0
7	2772.0
8	2699.0
9	2708.5
10	2718.0
11	2670.0
12	2622.0
13	2553.0
14	2484.0
15	2414.5
16	2345.0
17	2212.5
18	2080.0
19	1978.0
20	1876.0
21	1739.0
22	1602.0
23	1557.0
24	1512.0
25	1446.5
26	1372.0
27	1363.0
28	1441.0
29	1519.0
30	1554.0
31	1589.0
32	1725.0
33	1861.0
34	2116.0
35	2371.0
36	2563.5
37	2756.0
38	3240.5
39	3725.0
40	4406.0
41	5087.0
42	6480.0
43	7873.0
44	9496.5
45	11120.0
46	14712.5
47	18305.0
48	20493.0
49	22681.0
50	21794.5
51	20908.0
52	16688.5
53	12469.0
54	9191.0
55	5913.0
56	4364.0
57	2815.0
58	2294.0
59	1773.0
60	1501.0
61	1229.0
62	1049.5
63	870.0
64	774.0
65	678.0
66	544.5
67	411.0
68	322.0
69	233.0
70	194.0
71	155.0
72	136.0
73	117.0
74	89.5
75	49.0
76	36.0
77	23.0
78	10.0
79	7.5
80	5.0
81	3.5
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	162764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.20228060259025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57430469074305	48.29384876262564
2	9.733291822332918	11.524661473053008
3	3.6394769613947697	6.4639600894546705
4	1.7185554171855542	4.069696001572829
5	0.9371108343711083	2.773954928608292
6	0.5489829804898299	1.950062667420314
7	0.31963470319634707	1.3246172372269054
8	0.253217102532171	1.1992823965987565
9	0.17123287671232876	0.9123639133960826
>10	1.0107928601079286	10.778181907547124
>50	0.062266500622665005	2.5030104937209705
>100	0.028019925280199254	3.2261433732274947
>500	0.0020755500207555004	0.8023887346096189
>1k	0.0	0.0
>5k	0.0010377750103777502	4.177828020938291
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6800	4.177828020938291	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCC	782	0.4804502224079034	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	524	0.3219385122017154	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	461	0.28323216436066945	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	452	0.27770268609766285	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	419	0.2574279324666388	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	402	0.24698336241429308	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	395	0.2426826570986213	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	344	0.21134894694158413	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC	305	0.1873878744685557	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	173	0.10628885994445945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.143864736673957E-4	0.0	0.0	0.35327222235875255	0.0
2	6.143864736673957E-4	0.0	0.0	1.2447469956501438	0.0
3	6.143864736673957E-4	0.0	0.0	1.7632891794254257	0.0
4	6.143864736673957E-4	0.0	0.0	2.722960851293898	0.0
5	6.143864736673957E-4	0.0	0.0	5.011550465704947	0.0
6	6.143864736673957E-4	0.0	0.0	6.971443316703939	0.0
7	6.143864736673957E-4	0.0	0.0	8.60386817723821	0.0
8	6.143864736673957E-4	0.0	0.0	11.350175714531469	0.0
9	6.143864736673957E-4	0.0	0.0	12.492934555552825	0.0
10	6.143864736673957E-4	0.0	0.0	15.540905851416776	0.0
11	6.143864736673957E-4	0.0	0.0	22.431864540070286	0.0
12	6.143864736673957E-4	0.0	0.0	28.26730726696321	0.0
13	6.143864736673957E-4	0.0	0.0	30.184807451279152	0.0
14	6.143864736673957E-4	0.0	0.0	30.831142021577254	0.0
15	6.143864736673957E-4	0.0	0.0	31.757636823867685	0.0
16	6.143864736673957E-4	0.0	0.0	34.13224054459217	0.0
17	6.143864736673957E-4	0.0	0.0	37.31291931876828	0.0
18	6.143864736673957E-4	0.0	0.0	40.359047455211225	0.0
19	6.143864736673957E-4	0.0	0.0	42.203435649160745	0.0
20	6.143864736673957E-4	0.0	0.0	43.73694428743457	0.0
21	6.143864736673957E-4	0.0	0.0	45.64277112875083	0.0
22	6.143864736673957E-4	0.0	0.0	47.23526086849672	0.0
23	6.143864736673957E-4	0.0	0.0	48.55189108156595	0.0
24	6.143864736673957E-4	0.0	0.0	49.26457939102013	0.0
25	6.143864736673957E-4	0.0	0.0	49.77083384532206	0.0
26	6.143864736673957E-4	0.0	0.0	50.144380821311834	0.0
27	6.143864736673957E-4	0.0	0.0	50.47553513061857	0.0
28	6.143864736673957E-4	0.0	0.0	50.7593816814529	0.0
29	6.143864736673957E-4	0.0	0.0	51.04937209702391	0.0
30	6.143864736673957E-4	0.0	0.0	51.343048831436924	0.0
31	6.143864736673957E-4	0.0	0.0	51.60416308274557	0.0
32	6.143864736673957E-4	0.0	0.0	51.86404856110688	0.0
33	6.143864736673957E-4	0.0	0.0	52.088914010469146	0.0
34	6.143864736673957E-4	0.0	0.0	52.30763559509474	0.0
35	6.143864736673957E-4	0.0	0.0	52.5570765034037	0.0
36	6.143864736673957E-4	0.0	0.0	52.800987933449655	0.0
37	6.143864736673957E-4	0.0	0.0	53.05780147944263	0.0
38	6.143864736673957E-4	0.0	0.0	53.31092870659359	0.0
39	6.143864736673957E-4	0.0	0.0	53.57757243616525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGCG	25	3.876014E-5	45.0	5
GCGGGAA	55	1.8189894E-12	45.0	5
CCTATTA	20	7.015433E-4	45.0	15
GTTTGCG	20	7.015433E-4	45.0	1
CATTGAC	20	7.015433E-4	45.0	13
GTGCAGG	25	3.876014E-5	45.0	2
TTCGTTG	20	7.015433E-4	45.0	1
TGCGCGG	35	1.2033524E-7	45.0	2
GGCGCGA	20	7.015433E-4	45.0	8
ACCATAT	20	7.015433E-4	45.0	13
GCCTAGG	20	7.015433E-4	45.0	2
GGACCAT	20	7.015433E-4	45.0	7
GCGGCGA	25	3.876014E-5	45.0	9
TGTTTCG	20	7.015433E-4	45.0	1
TGCTGCG	20	7.015433E-4	45.0	1
AGGGTAG	20	7.015433E-4	45.0	6
GCGCGAC	20	7.015433E-4	45.0	9
CGTAGAG	25	3.876014E-5	45.0	38
AGGGACC	35	1.2033524E-7	45.0	6
CTGAATG	25	3.876014E-5	45.0	5
>>END_MODULE