##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135449
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871996101853835	33.0	31.0	34.0	30.0	34.0
2	32.02703600617207	33.0	31.0	34.0	30.0	34.0
3	32.15063972417663	33.0	31.0	34.0	30.0	34.0
4	35.82885809419043	37.0	35.0	37.0	35.0	37.0
5	35.81991745970808	37.0	35.0	37.0	35.0	37.0
6	35.706420866894554	37.0	35.0	37.0	35.0	37.0
7	35.80527726302889	37.0	35.0	37.0	35.0	37.0
8	35.47179381169296	37.0	35.0	37.0	33.0	37.0
9	37.350190846739366	39.0	37.0	39.0	34.0	39.0
10	36.973569387740035	39.0	37.0	39.0	33.0	39.0
11	37.01907729108373	39.0	37.0	39.0	33.0	39.0
12	36.98698403088986	39.0	37.0	39.0	33.0	39.0
13	36.841623046312634	39.0	37.0	39.0	33.0	39.0
14	37.67131540284535	40.0	37.0	41.0	32.0	41.0
15	37.76614814432	40.0	37.0	41.0	32.0	41.0
16	37.91551801785174	40.0	37.0	41.0	33.0	41.0
17	37.88493824243811	40.0	37.0	41.0	33.0	41.0
18	37.673987995481696	39.0	37.0	40.0	33.0	41.0
19	37.40325879113172	38.0	37.0	40.0	33.0	41.0
20	36.849854926946676	38.0	35.0	40.0	32.0	41.0
21	36.77855133666546	38.0	35.0	40.0	32.0	41.0
22	36.8348751190485	38.0	35.0	40.0	32.0	41.0
23	36.71547962701829	38.0	35.0	40.0	32.0	41.0
24	36.626073282194774	38.0	35.0	40.0	32.0	41.0
25	36.55383945248765	38.0	35.0	40.0	32.0	41.0
26	36.4972498874115	38.0	35.0	40.0	32.0	41.0
27	36.41165309452266	38.0	35.0	40.0	31.0	41.0
28	36.30016463761268	38.0	35.0	40.0	31.0	41.0
29	36.18347126962916	38.0	35.0	40.0	31.0	41.0
30	36.06886724892764	38.0	35.0	40.0	31.0	41.0
31	35.78642884037534	38.0	35.0	40.0	30.0	41.0
32	35.31168188764775	38.0	35.0	40.0	27.0	41.0
33	35.03801430796831	38.0	35.0	40.0	24.0	41.0
34	34.66986836373838	38.0	34.0	40.0	22.0	41.0
35	34.28871383325089	38.0	34.0	40.0	19.0	41.0
36	34.16810755339648	38.0	34.0	40.0	18.0	41.0
37	33.976633271563465	38.0	33.0	40.0	18.0	41.0
38	33.87427740330309	38.0	33.0	40.0	18.0	41.0
39	33.85571691190042	38.0	33.0	40.0	17.0	41.0
40	33.70205021816329	38.0	33.0	40.0	15.0	41.0
41	33.387887692046455	37.0	33.0	40.0	15.0	41.0
42	33.27292929442078	37.0	33.0	40.0	15.0	41.0
43	33.26728879504463	37.0	33.0	40.0	15.0	41.0
44	33.2262032204003	37.0	33.0	40.0	15.0	41.0
45	33.24863232655834	37.0	33.0	40.0	15.0	41.0
46	32.996589122105	37.0	32.0	40.0	13.0	41.0
47	32.76637701275018	36.0	32.0	40.0	12.0	41.0
48	32.723305450760066	36.0	31.0	40.0	12.0	41.0
49	32.820596682146046	36.0	32.0	40.0	12.0	41.0
50	32.72605187192227	36.0	32.0	40.0	12.0	41.0
51	31.654327459043625	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	4.0
13	1.0
14	6.0
15	13.0
16	20.0
17	26.0
18	50.0
19	139.0
20	229.0
21	397.0
22	629.0
23	1025.0
24	1443.0
25	2352.0
26	3291.0
27	3482.0
28	3426.0
29	3392.0
30	3342.0
31	4083.0
32	4716.0
33	6296.0
34	8982.0
35	11195.0
36	14076.0
37	17274.0
38	26818.0
39	18730.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.157084954484713	4.753080495241751	37.45764088328448	30.63219366698905
2	35.02351438548826	10.291696505695871	36.70901963100503	17.97576947781084
3	17.279566478896115	10.235586826037844	54.50833893199655	17.976507763069492
4	14.637243538158273	5.005574053702869	59.82989907640515	20.527283331733713
5	16.77014965042193	5.840574681245339	55.45925034514836	21.93002532318437
6	16.29543222910468	11.124482277462366	58.83247569195787	13.747609801475095
7	57.1329430265266	2.890386787647011	33.48640447696181	6.490265708864591
8	54.160606575168515	7.658232988061927	29.794978183670608	8.386182253098951
9	49.38833066320164	5.939504905905544	32.22024525836293	12.451919172529882
10	29.230189960797055	17.796366159956882	37.38381235741866	15.589631521827405
11	21.77129399257285	16.868341589823476	40.0032484551381	21.357115962465578
12	17.98241404513876	14.186889530376748	46.55110041417803	21.279596010306463
13	21.77424713360748	16.11159919969878	47.94055327097284	14.173600395720898
14	16.333823062554913	20.293246904739053	45.48427821541687	17.888651817289166
15	11.837665837326226	17.42057896329984	49.56699569579694	21.174759503576993
16	12.76790526323561	18.676402188277507	43.19707048409364	25.358622064393238
17	12.804081240909863	19.774970653160967	43.01914373675701	24.40180436917216
18	14.214944370205759	17.02928777621097	46.17383664700367	22.581931206579597
19	13.612503599140634	19.79047464359279	45.33071488161596	21.266306875650614
20	14.909670798603164	23.887219543887365	44.445510856484724	16.757598801024738
21	13.984599369504389	25.552791087420356	43.95824258577029	16.504366957304963
22	10.993805786679857	22.038553256207134	43.006592887359815	23.96104806975319
23	12.404668915975755	23.619221994994426	40.69649831301818	23.279610776011637
24	14.754630894284935	23.057386913155504	38.38271231238326	23.8052698801763
25	13.040332523680501	26.296982628147862	36.247591344343626	24.415093503828007
26	12.86388234686118	22.745830534001726	41.52854579952602	22.86174131961107
27	17.154796270182874	21.64061750179034	40.51118871309497	20.693397514931817
28	11.323081012041433	21.760957998951636	40.601259514651275	26.31470147435566
29	15.065448988180052	20.28660233741113	40.69871316879416	23.94923550561466
30	21.75062200533042	21.394768510657148	36.723785336178196	20.13082414783424
31	19.7779237941956	24.543555138834545	31.56538623393307	24.11313483303679
32	18.37370523222763	27.072920434997677	31.744051266528363	22.809323066246336
33	21.26409201987464	19.847322608509476	31.130536216583366	27.75804915503252
34	19.823697480232415	19.726982111348182	34.103610953200096	26.345709455219307
35	19.596305620565673	22.77388537383074	33.790578003529006	23.83923100207458
36	22.88758130366411	21.412487356864947	34.212877171481516	21.487054167989427
37	21.942576172581564	24.361198679945957	31.31289267547195	22.383332472000532
38	17.741733050816176	27.340179698631957	28.0784649572902	26.839622293261673
39	21.239728606338918	22.80710821047036	30.593064548280164	25.360098634910557
40	24.265959881579043	20.677893524499996	31.404440047545574	23.65170654637539
41	21.908615050683284	24.387038663998997	30.70823704863085	22.99610923668687
42	19.796380925662056	23.040406352206368	37.423679761386204	19.739532960745372
43	19.50549653375071	19.277366388825314	39.17120096863026	22.045936108793715
44	20.06068704826171	18.822582669491837	34.1619354886341	26.954794793612358
45	22.47635641459147	17.023381494141706	31.772844391616033	28.727417699650793
46	24.15817023381494	20.00826879489697	34.73853627564619	21.0950246956419
47	16.214959135910934	23.394044991103662	40.07117069893466	20.31982517405075
48	17.436821238990323	22.449778145279772	37.876248624943706	22.2371519907862
49	21.396983366433123	17.46266122304336	40.19446433713058	20.94589107339294
50	20.547217033717487	15.673796041314441	35.93529667993119	27.84369024503688
51	18.286587571705955	16.04736838219551	33.002089347282	32.663954698816525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	534.0
1	768.0
2	1002.0
3	5035.0
4	9068.0
5	5585.0
6	2102.0
7	2004.5
8	1907.0
9	1866.5
10	1826.0
11	1768.0
12	1710.0
13	1600.0
14	1490.0
15	1405.0
16	1320.0
17	1192.5
18	1065.0
19	1021.5
20	978.0
21	900.0
22	822.0
23	796.0
24	770.0
25	750.0
26	742.5
27	755.0
28	777.0
29	799.0
30	837.0
31	875.0
32	936.0
33	997.0
34	1174.5
35	1352.0
36	1446.5
37	1541.0
38	1769.5
39	1998.0
40	2802.5
41	3607.0
42	4674.5
43	5742.0
44	6429.5
45	7117.0
46	11530.0
47	15943.0
48	16965.5
49	17988.0
50	17732.5
51	17477.0
52	14226.5
53	10976.0
54	8674.5
55	6373.0
56	5321.0
57	4269.0
58	3669.0
59	3069.0
60	2712.5
61	2356.0
62	2105.5
63	1855.0
64	1700.0
65	1545.0
66	1337.5
67	1130.0
68	972.5
69	815.0
70	672.5
71	530.0
72	462.5
73	395.0
74	334.0
75	223.5
76	174.0
77	135.5
78	97.0
79	68.0
80	39.0
81	31.0
82	23.0
83	16.0
84	9.0
85	6.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	135449.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.51609831006504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7808394063514	44.29120923742516
2	11.20671312303846	12.443059749425982
3	4.226288632374063	7.038811656047664
4	1.9070163306558858	4.234804243663667
5	0.8737166870578221	2.4252670746923197
6	0.45215171019735095	1.5061019276628105
7	0.29522846959944676	1.1472952919549055
8	0.21410713335815734	0.9509114131518136
9	0.14362466088621736	0.7176132714158097
>10	0.7925953508165329	7.782264911516512
>50	0.05984360870259056	2.3012351512377354
>100	0.034576307250385664	3.647129177771707
>500	0.007979147827012076	3.860493617523939
>1k	0.003989573913506038	2.9686450250647844
>5k	0.0013298579711686792	4.685158251445194
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6346	4.685158251445194	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	1742	1.2860929205826548	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	1200	0.8859423103898885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1079	0.7966097940922413	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	979	0.7227812682264173	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	963	0.7109687040878855	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	962	0.7102304188292272	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	872	0.6437847455499855	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	792	0.5847219248573263	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	661	0.48800655597309683	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	405	0.29900552975658734	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	383	0.2827632540661061	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	328	0.24215756483990286	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	324	0.2392044238052699	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	317	0.2340364269946622	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTA	278	0.2052433019069908	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	255	0.1882627409578513	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.17054389475005352	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	203	0.1498719075076228	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	187	0.13805934336909095	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	176	0.1299382055238503	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	172	0.12698506448921734	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	156	0.11517250035068549	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATG	136	0.10040679517752069	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	136	0.10040679517752069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8187583518519885	0.0
2	0.0	0.0	0.0	2.8918633581643274	0.0
3	0.0	0.0	0.0	3.888548457352952	0.0
4	0.0	0.0	0.0	5.302364727683482	0.0
5	0.0	0.0	0.0	8.621695250610932	0.0
6	0.0	0.0	0.0	10.4644552562219	0.0
7	0.0	0.0	0.0	12.493263147014744	0.0
8	0.0	0.0	0.0	15.196125478962562	0.0
9	0.0	0.0	0.0	16.37516703703977	0.0
10	0.0	0.0	0.0	19.94108483635907	0.0
11	0.0	0.0	0.0	25.935222851405324	0.0
12	0.0	0.0	0.0	31.436924598926534	0.0
13	0.0	0.0	0.0	33.16598867470413	0.0
14	0.0	0.0	0.0	33.958907042503085	0.0
15	0.0	0.0	0.0	35.08110063566361	0.0
16	0.0	0.0	0.0	37.128365657922906	0.0
17	0.0	0.0	0.0	39.48054249200806	0.0
18	0.0	0.0	0.0	41.59277661702929	0.0
19	0.0	0.0	0.0	43.20371505142157	0.0
20	0.0	0.0	0.0	44.347318917083186	0.0
21	0.0	0.0	0.0	45.53595818352295	0.0
22	0.0	0.0	0.0	46.591706103404235	0.0
23	7.382852586582403E-4	0.0	0.0	47.32260850947589	0.0
24	7.382852586582403E-4	0.0	0.0	47.729403686996584	0.0
25	7.382852586582403E-4	0.0	0.0	48.02840921675317	0.0
26	7.382852586582403E-4	0.0	0.0	48.26318392900649	0.0
27	7.382852586582403E-4	0.0	0.0	48.540779186261986	0.0
28	7.382852586582403E-4	0.0	0.0	48.70984651049473	0.0
29	7.382852586582403E-4	0.0	0.0	48.909921815591105	0.0
30	7.382852586582403E-4	0.0	0.0	49.10261426810091	0.0
31	7.382852586582403E-4	0.0	0.0	49.247318178797926	0.0
32	7.382852586582403E-4	0.0	0.0	49.414170647254686	0.0
33	7.382852586582403E-4	0.0	0.0	49.560351128469016	0.0
34	7.382852586582403E-4	0.0	0.0	49.74861386942687	0.0
35	7.382852586582403E-4	0.0	0.0	49.912513196849	0.0
36	7.382852586582403E-4	0.0	0.0	50.0520491107354	0.0
37	7.382852586582403E-4	0.0	0.0	50.1812490310006	0.0
38	7.382852586582403E-4	0.0	0.0	50.38132433609698	0.0
39	7.382852586582403E-4	0.0	0.0	50.727580122407694	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.011233E-4	45.000004	1
TCGCATT	20	7.011233E-4	45.000004	22
ACACGTG	55	1.8189894E-12	45.000004	42
TTTGTCG	25	3.8725375E-5	45.000004	1
ATGGAAG	20	7.011233E-4	45.000004	40
TATGGAA	20	7.011233E-4	45.000004	39
GGGTAAC	20	7.011233E-4	45.000004	7
TCGGGTA	20	7.011233E-4	45.000004	5
CCTACAC	45	3.8016879E-10	45.000004	28
ATTCGAG	20	7.011233E-4	45.000004	14
GTAGGAT	20	7.011233E-4	45.000004	33
ATTTTGA	20	7.011233E-4	45.000004	26
GGGAATC	25	3.8725375E-5	45.000004	7
CTCGGGT	20	7.011233E-4	45.000004	4
TAATTAT	45	3.8016879E-10	45.000004	40
TTAAGCA	20	7.011233E-4	45.000004	45
CGAGGGC	20	7.011233E-4	45.000004	4
ACGTGAG	55	1.8189894E-12	45.000004	44
TCCCGGG	20	7.011233E-4	45.000004	3
ATTGGGA	45	3.8016879E-10	45.000004	4
>>END_MODULE