##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136312
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816487176477494	33.0	31.0	34.0	30.0	34.0
2	31.993074710957217	33.0	31.0	34.0	30.0	34.0
3	32.13000322788896	33.0	31.0	34.0	30.0	34.0
4	35.75460707788016	37.0	35.0	37.0	35.0	37.0
5	35.82127032102823	37.0	35.0	37.0	35.0	37.0
6	35.735782616350726	37.0	35.0	37.0	35.0	37.0
7	35.72697928282176	37.0	35.0	37.0	35.0	37.0
8	35.45087739890839	37.0	35.0	37.0	33.0	37.0
9	37.31716209871471	39.0	37.0	39.0	34.0	39.0
10	37.03984242032983	39.0	37.0	39.0	33.0	39.0
11	37.0566714595927	39.0	37.0	39.0	33.0	39.0
12	36.98495363577675	39.0	37.0	39.0	33.0	39.0
13	36.86062855801397	39.0	37.0	39.0	33.0	39.0
14	37.713282763072954	40.0	37.0	41.0	33.0	41.0
15	37.78153060625623	40.0	37.0	41.0	32.0	41.0
16	38.02702623393392	40.0	37.0	41.0	33.0	41.0
17	38.0136158225248	40.0	37.0	41.0	33.0	41.0
18	37.86359968307999	39.0	37.0	40.0	33.0	41.0
19	37.623158636070194	39.0	37.0	40.0	33.0	41.0
20	37.04695844826574	39.0	35.0	40.0	33.0	41.0
21	37.02099595046658	38.0	35.0	40.0	33.0	41.0
22	37.04568196490404	38.0	35.0	40.0	33.0	41.0
23	36.84386554375257	38.0	35.0	40.0	32.0	41.0
24	36.755377369563945	38.0	35.0	40.0	32.0	41.0
25	36.72035477434122	38.0	35.0	40.0	32.0	41.0
26	36.70274810728329	38.0	35.0	40.0	32.0	41.0
27	36.612763366394745	38.0	35.0	40.0	32.0	41.0
28	36.57121896824931	38.0	35.0	40.0	32.0	41.0
29	36.38243881683197	38.0	35.0	40.0	31.0	41.0
30	36.19114971535888	38.0	35.0	40.0	31.0	41.0
31	35.9251203122249	38.0	35.0	40.0	30.0	41.0
32	35.55008363166853	38.0	35.0	40.0	29.0	41.0
33	35.17375579552791	38.0	35.0	40.0	25.0	41.0
34	34.80463935676976	38.0	35.0	40.0	22.0	41.0
35	34.502545630612126	38.0	34.0	40.0	21.0	41.0
36	34.311491284699805	38.0	34.0	40.0	18.0	41.0
37	34.16459299254651	38.0	34.0	40.0	18.0	41.0
38	34.056422031809376	38.0	34.0	40.0	18.0	41.0
39	34.061307881917955	38.0	34.0	40.0	18.0	41.0
40	33.91457098421269	38.0	33.0	40.0	18.0	41.0
41	33.650522331122716	37.0	33.0	40.0	15.0	41.0
42	33.43197224015494	37.0	33.0	40.0	15.0	41.0
43	33.43159809847996	37.0	33.0	40.0	15.0	41.0
44	33.39552643934503	37.0	33.0	40.0	15.0	41.0
45	33.403962967310285	37.0	33.0	40.0	15.0	41.0
46	33.14052321145607	37.0	33.0	40.0	15.0	41.0
47	33.00281706672927	37.0	32.0	40.0	13.0	41.0
48	32.944428957098424	36.0	32.0	40.0	13.0	41.0
49	33.019660778214686	37.0	32.0	40.0	13.0	41.0
50	32.83030107400669	36.0	32.0	40.0	12.0	41.0
51	31.877032102822934	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	8.0
13	8.0
14	14.0
15	10.0
16	15.0
17	30.0
18	63.0
19	120.0
20	249.0
21	386.0
22	655.0
23	987.0
24	1458.0
25	2211.0
26	3149.0
27	3462.0
28	3505.0
29	3248.0
30	3347.0
31	3792.0
32	4512.0
33	5985.0
34	8952.0
35	10883.0
36	13777.0
37	16394.0
38	26477.0
39	22601.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.236780327484006	4.753066494512589	37.194817770995954	30.815335407007456
2	35.14584189212982	10.296965784377017	36.408386642408594	18.14880568108457
3	17.194377604319502	10.470831621574037	54.29309231762427	18.041698456482187
4	14.823346440518808	5.10373261341628	59.60370326897119	20.469217677093727
5	17.016843711485414	5.94371735430483	54.98928927753977	22.050149656669994
6	16.29423675098304	11.227918305064852	58.727771582839374	13.750073361112742
7	56.975908210575746	3.076765068372557	33.478343799518754	6.4689829215329535
8	54.1162920359176	7.735195727448795	29.70758260461295	8.440929632020659
9	49.181289981806444	6.009742355772053	32.151974881155	12.656992781266506
10	29.22633370502964	17.647015669933683	37.35914666353659	15.767503961500088
11	22.192470215388227	16.967691765948707	39.906978109043955	20.932859909619108
12	18.22803568284524	14.27387170608604	46.001819355595984	21.49627325547274
13	21.858677152415048	16.182727859616175	47.71406772697928	14.244527260989495
14	16.427753976172312	20.40906156464581	45.29168378425964	17.871500674922235
15	11.833881096308469	17.357972885732732	49.67721110393802	21.130934914020777
16	13.08395445742121	18.553759023416866	42.94412817653618	25.41815834262574
17	13.111831680262926	19.877193497270966	42.86563178590293	24.14534303656318
18	14.262867539174835	17.113680380304007	45.88664240859205	22.7368096719291
19	13.758876694641705	20.022448500498854	45.16110100358002	21.057573801279418
20	14.76612477258055	23.894448030987732	44.461969599154884	16.877457597276834
21	14.017841422618698	25.738746405305474	43.715153471447856	16.52825870062797
22	11.205176360115031	21.93277187628382	43.22730207171782	23.63474969188333
23	12.849932507776277	23.757262750161395	40.37575561946124	23.01704912260109
24	14.69423088209402	23.141029403133988	38.39500557544457	23.769734139327426
25	13.338517518633722	26.566259757027993	36.00269968894888	24.0925230353894
26	12.882211397382475	22.645108281002408	41.36906508597922	23.103615235635893
27	17.290480662010683	21.70095076002113	40.347144785492105	20.661423792476082
28	11.704765537883679	21.653266036739247	40.762368683608194	25.87959974176888
29	15.231967838488176	20.475820177240447	40.49093256646517	23.80127941780621
30	21.64226186982804	21.640794647573212	36.63433886965197	20.08260461294677
31	19.93734960971888	24.36762720816949	31.679529315100652	24.015493867010974
32	18.616849580374435	27.10619754680439	31.504929866776223	22.772023006044957
33	21.262984916955222	20.00704266682317	31.087505135277894	27.642467280943716
34	20.28801572862257	19.858119607958212	34.065966312577025	25.787898350842188
35	19.52726098949469	22.894536064323024	33.69989435999765	23.878308586184634
36	22.69719467104877	21.61511825811374	34.22369270497095	21.463994365866544
37	22.110305769117904	23.96707553260168	31.46017958800399	22.462439110276424
38	17.79153706203416	27.084922824109398	28.17140090380891	26.952139210047537
39	21.101590468924233	23.325165796114796	30.52335817829685	25.049885556664126
40	24.283995539644344	20.720846293796587	31.538675978637244	23.456482187921825
41	21.900492986677623	24.421180820470685	30.926844298374316	22.751481894477376
42	19.579347379541055	23.244468572099304	37.28578555079524	19.89039849756441
43	19.88819766418217	19.194201537648922	38.922471976054936	21.995128822113973
44	20.139092669757616	18.889019308644876	34.393156875403484	26.578731146194023
45	22.28710605082458	17.292681495392923	31.736017371911494	28.684195081871
46	24.192294148717647	20.358442396854276	34.43497270966606	21.014290744762015
47	16.30230647338459	23.274546628323257	40.20188978226422	20.221257116027935
48	17.432067609601503	22.768354950407886	37.34300721873349	22.456570221257117
49	21.106725746816128	17.647015669933683	40.04196255648806	21.204296026762133
50	20.929925465109456	15.867275074828335	35.96308468806855	27.23971477199366
51	18.36962263043606	16.170256470450145	33.12107518046834	32.33904571864546
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	451.0
1	735.0
2	1019.0
3	5094.0
4	9169.0
5	5648.0
6	2127.0
7	2060.5
8	1994.0
9	1971.0
10	1948.0
11	1827.0
12	1706.0
13	1593.0
14	1480.0
15	1378.0
16	1276.0
17	1184.5
18	1093.0
19	1014.0
20	935.0
21	866.5
22	798.0
23	767.5
24	737.0
25	729.0
26	743.5
27	766.0
28	776.5
29	787.0
30	862.0
31	937.0
32	1003.5
33	1070.0
34	1226.5
35	1383.0
36	1469.0
37	1555.0
38	1786.0
39	2017.0
40	2788.5
41	3560.0
42	4686.0
43	5812.0
44	6480.5
45	7149.0
46	11620.5
47	16092.0
48	16993.5
49	17895.0
50	17614.5
51	17334.0
52	14023.5
53	10713.0
54	8666.0
55	6619.0
56	5515.5
57	4412.0
58	3800.5
59	3189.0
60	2852.5
61	2516.0
62	2191.5
63	1867.0
64	1734.0
65	1601.0
66	1385.0
67	1169.0
68	979.0
69	789.0
70	693.0
71	597.0
72	494.0
73	391.0
74	335.5
75	231.0
76	182.0
77	137.0
78	92.0
79	69.5
80	47.0
81	34.0
82	21.0
83	13.0
84	5.0
85	6.5
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	136312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.791859850930216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91084929849707	44.583749046305535
2	11.354222824157473	12.669464170432537
3	4.099880343453735	6.862198485826633
4	1.7843289371605897	3.9820411996009155
5	0.9033411789457074	2.5199542226656493
6	0.4391789720056278	1.4701566993368156
7	0.28401993399166353	1.109220024649334
8	0.2038106007810548	0.9096777979928399
9	0.13280561728313894	0.6668525148189447
>10	0.7797399113752613	7.7645401725453365
>50	0.061800633785223075	2.367363108163625
>100	0.03287267754533142	3.6093667468748167
>500	0.007889442610879541	3.840454252010095
>1k	0.003944721305439771	2.976260343916896
>5k	0.001314907101813257	4.668701214860027
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6364	4.668701214860027	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	1706	1.2515405833675686	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1197	0.8781325195140559	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	1154	0.8465872410352719	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	997	0.7314102940313398	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	982	0.7204061271201361	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	938	0.6881272375139387	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	871	0.6389752919772287	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	786	0.5766183461470744	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	661	0.48491695522037676	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	405	0.29711250660250016	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	341	0.250161394448031	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	325	0.23842361640941367	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	304	0.2230177827337285	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	299	0.2193497270966606	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTA	295	0.2164152825870063	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	258	0.1892716708727038	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	249	0.18266917072598157	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.17973472621632725	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	235	0.17239861494219144	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	168	0.12324666940548154	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	168	0.12324666940548154	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	151	0.11077528023945069	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	149	0.10930805798462351	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	141	0.10343916896531485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7108691824637596	0.0
2	0.0	0.0	0.0	2.723898116086625	0.0
3	0.0	0.0	0.0	3.7568225834849462	0.0
4	0.0	0.0	0.0	5.174892892775397	0.0
5	0.0	0.0	0.0	8.472474910499443	0.0
6	0.0	0.0	0.0	10.333646340747697	0.0
7	0.0	0.0	0.0	12.249104994424556	0.0
8	0.0	0.0	0.0	14.89524033100534	0.0
9	0.0	0.0	0.0	16.186395915253243	0.0
10	0.0	0.0	0.0	19.765684605904102	0.0
11	0.0	0.0	0.0	25.582487235166383	0.0
12	7.336111274135806E-4	0.0	0.0	31.130788191795293	0.0
13	7.336111274135806E-4	0.0	0.0	32.82542989612067	0.0
14	7.336111274135806E-4	0.0	0.0	33.65514408122542	0.0
15	7.336111274135806E-4	0.0	0.0	34.81278244028405	0.0
16	7.336111274135806E-4	0.0	0.0	36.79940137332003	0.0
17	7.336111274135806E-4	0.0	0.0	39.05745642349903	0.0
18	7.336111274135806E-4	0.0	0.0	41.077087857268616	0.0
19	7.336111274135806E-4	0.0	0.0	42.620605669346794	0.0
20	7.336111274135806E-4	0.0	0.0	43.72469041610423	0.0
21	7.336111274135806E-4	0.0	0.0	44.94175127648336	0.0
22	7.336111274135806E-4	0.0	0.0	45.904982686777394	0.0
23	7.336111274135806E-4	0.0	0.0	46.624655202770114	0.0
24	7.336111274135806E-4	0.0	0.0	47.020805211573446	0.0
25	7.336111274135806E-4	0.0	0.0	47.3259874405775	0.0
26	7.336111274135806E-4	0.0	0.0	47.56587827924174	0.0
27	7.336111274135806E-4	0.0	0.0	47.82044134045425	0.0
28	7.336111274135806E-4	0.0	0.0	48.01118023358178	0.0
29	7.336111274135806E-4	0.0	0.0	48.20705440460121	0.0
30	7.336111274135806E-4	0.0	0.0	48.405129409002875	0.0
31	7.336111274135806E-4	0.0	0.0	48.56725746816128	0.0
32	7.336111274135806E-4	0.0	0.0	48.74405774986795	0.0
33	7.336111274135806E-4	0.0	0.0	48.89224719760549	0.0
34	7.336111274135806E-4	0.0	0.0	49.07271553494923	0.0
35	7.336111274135806E-4	0.0	0.0	49.26345442807676	0.0
36	7.336111274135806E-4	0.0	0.0	49.394770819883796	0.0
37	7.336111274135806E-4	0.0	0.0	49.540025823111684	0.0
38	7.336111274135806E-4	0.0	0.0	49.72269499383766	0.0
39	7.336111274135806E-4	0.0	0.0	50.06309055695757	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.011398E-4	45.000004	5
CATGGGA	20	7.011398E-4	45.000004	4
TGGGTCC	20	7.011398E-4	45.000004	6
GGTCCAG	20	7.011398E-4	45.000004	8
GCACGGG	20	7.011398E-4	45.000004	3
TGTATTG	20	7.011398E-4	45.000004	1
GGTCAAG	40	6.7393557E-9	45.000004	15
ACGGGCT	20	7.011398E-4	45.000004	5
CTGAATG	20	7.011398E-4	45.000004	5
TGCATGG	20	7.011398E-4	45.000004	2
TACTGAT	20	7.011398E-4	45.000004	20
GCCCTAC	20	7.011398E-4	45.000004	26
GCGGGAT	20	7.011398E-4	45.000004	5
GTCAAGC	35	1.2014243E-7	45.000004	16
ACATGGG	20	7.011398E-4	45.000004	3
CTGGGTC	35	1.2014243E-7	45.000004	5
CTGGGTA	45	3.8016879E-10	45.000004	5
GTTGGGT	20	7.011398E-4	45.000004	4
TGGTCAA	35	1.2014243E-7	45.000004	14
TTGGGTC	40	6.7393557E-9	45.000004	5
>>END_MODULE