FastQCFastQC Report
Sat 14 Jan 2017
SRR2933625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237857
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72193.0350168378479503No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT16020.6735139180263772No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC14990.6302105887150683No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC12360.5196399517357068No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC10850.456156430123982No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC10720.45069096137595277No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC6430.2703304926909866No Hit
TCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG4550.19129140618102475No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG3520.14798807686971585No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC3340.14042050475705992No Hit
GCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG3250.13663671870073196No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.1286487259151507No Hit
TTCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT3040.12780788456930003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.12696704322344937No Hit
TGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG3000.12612620187759874No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC2860.12024031245664413No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG2790.11729736774616681No Hit
GTGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC2620.11015021630643622No Hit
GGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT2500.10510516823133227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCT207.0219947E-445.00000433
GATCCTA207.0219947E-445.0000041
GGGTACC351.2064993E-745.0000047
GGCCGAT207.0219947E-445.0000048
TTCGTTG351.2064993E-745.0000041
TAACGTC207.0219947E-445.00000432
TTCCGAT207.0219947E-445.00000413
GCGTTTG302.15822E-645.0000041
TACGGGA207.0219947E-445.0000044
TGTTCGG302.15822E-645.0000042
CTTTGCG406.7757355E-945.0000041
ATTGGGT207.0219947E-445.0000044
ATTGGGC406.7757355E-945.0000044
CGTCGGG207.0219947E-445.0000043
TTACGGG302.15822E-645.0000043
ATAACGT207.0219947E-445.00000431
TGGGCGC351.2064993E-745.0000046
GTTTGCG253.88145E-545.01
TTGCTCG253.88145E-545.01
TACGAAT253.88145E-545.012
Produced by FastQC (version 0.11.5)