##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933625.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237857
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.700677297704082	33.0	31.0	34.0	30.0	34.0
2	31.89539092816272	33.0	31.0	34.0	30.0	34.0
3	32.054305738321766	33.0	31.0	34.0	30.0	34.0
4	35.651656247240986	37.0	35.0	37.0	33.0	37.0
5	35.728126563439375	37.0	35.0	37.0	35.0	37.0
6	35.70407850094805	37.0	35.0	37.0	35.0	37.0
7	35.668216617547515	37.0	35.0	37.0	35.0	37.0
8	35.45432339599003	37.0	35.0	37.0	33.0	37.0
9	37.274442206872195	39.0	37.0	39.0	34.0	39.0
10	36.85120051123154	39.0	37.0	39.0	32.0	39.0
11	36.883047377205635	39.0	37.0	39.0	33.0	39.0
12	36.939665429228484	39.0	37.0	39.0	33.0	39.0
13	36.92072127370647	39.0	37.0	39.0	33.0	39.0
14	37.903517659770365	39.0	37.0	41.0	33.0	41.0
15	37.89004317720311	39.0	37.0	41.0	33.0	41.0
16	38.076255901655195	40.0	37.0	41.0	33.0	41.0
17	38.11361027844461	40.0	37.0	41.0	33.0	41.0
18	38.041234859600515	39.0	37.0	40.0	34.0	41.0
19	37.866625745721166	39.0	37.0	40.0	34.0	41.0
20	37.46715043072098	39.0	35.0	40.0	33.0	41.0
21	37.379778606473636	39.0	35.0	40.0	33.0	41.0
22	37.387884317047636	39.0	35.0	40.0	33.0	41.0
23	37.2972079863111	39.0	35.0	40.0	33.0	41.0
24	37.23022656470064	39.0	35.0	40.0	33.0	41.0
25	37.14523011725532	39.0	35.0	40.0	33.0	41.0
26	37.1511622529503	39.0	35.0	40.0	33.0	41.0
27	37.0592835190892	39.0	35.0	40.0	33.0	41.0
28	37.068448689758974	39.0	35.0	40.0	33.0	41.0
29	36.96875433558819	39.0	35.0	40.0	32.0	41.0
30	36.74010014420429	39.0	35.0	40.0	31.0	41.0
31	36.43451317388178	38.0	35.0	40.0	31.0	41.0
32	36.20059111146613	38.0	35.0	40.0	30.0	41.0
33	35.80043891918253	38.0	35.0	40.0	29.0	41.0
34	35.58955590964319	38.0	35.0	40.0	27.0	41.0
35	35.43036782604674	38.0	35.0	40.0	25.0	41.0
36	35.168891392727566	38.0	35.0	40.0	24.0	41.0
37	34.939211374901724	38.0	35.0	40.0	23.0	41.0
38	34.8062827665362	38.0	34.0	40.0	23.0	41.0
39	34.86997229427765	38.0	34.0	40.0	23.0	41.0
40	34.76353018830642	38.0	34.0	40.0	22.0	41.0
41	34.65573012356164	38.0	34.0	40.0	22.0	41.0
42	34.370117339409816	38.0	34.0	40.0	21.0	41.0
43	34.207557482016504	38.0	33.0	40.0	20.0	41.0
44	34.14240909454	38.0	33.0	40.0	20.0	41.0
45	34.1122144818105	38.0	33.0	40.0	20.0	41.0
46	33.74562867605326	37.0	33.0	40.0	20.0	41.0
47	33.56390184018129	37.0	32.0	40.0	20.0	41.0
48	33.50411381628457	37.0	32.0	40.0	19.0	41.0
49	33.58841656961956	37.0	33.0	40.0	20.0	41.0
50	33.399899939879845	36.0	32.0	40.0	18.0	41.0
51	32.393509545651376	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	1.0
12	7.0
13	18.0
14	11.0
15	13.0
16	23.0
17	29.0
18	80.0
19	140.0
20	268.0
21	414.0
22	590.0
23	993.0
24	1697.0
25	2855.0
26	4440.0
27	5198.0
28	5277.0
29	5215.0
30	5635.0
31	6216.0
32	8017.0
33	9986.0
34	14330.0
35	18600.0
36	24670.0
37	33420.0
38	53280.0
39	36416.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.99719158990486	5.584027377794221	42.65251810962049	27.766262922680436
2	31.91161075772418	6.079282930500258	40.934679240047586	21.07442707172797
3	20.375267492653148	6.506850754865318	51.88579692840657	21.23208482407497
4	16.709619645417202	6.398802641923508	52.46303451233304	24.428543200326246
5	14.424633288068042	6.71916319469261	53.06633817798089	25.789865339258462
6	17.70559621957731	8.336521523436351	58.708804029311736	15.249078227674612
7	65.61757694749366	3.2860079795843724	24.503378080106955	6.59303699281501
8	62.88021794607685	4.272314878267195	24.180074582627377	8.667392593028584
9	56.77739145789277	6.05573937281644	27.86380051879911	9.303068650491682
10	29.36133895575913	20.062054091323777	33.024464278957524	17.552142673959565
11	18.59310426012268	17.736707349373784	38.08759044299727	25.582597947506276
12	16.153823515809922	14.710939766330188	45.40585309660847	23.729383621251422
13	20.73430674733138	16.378328155152044	46.97360178594702	15.913763311569557
14	18.506918022172986	18.16974064248687	44.932459418894545	18.390881916445593
15	12.698806425709567	17.281391760595653	46.976544730657494	23.04325708303729
16	11.937845007714719	17.523133647527718	43.690116330400194	26.848905014357367
17	11.590997952551323	18.239530474192478	41.97353872284608	28.19593285041012
18	13.255863817335625	16.088658311506492	45.75816562051989	24.897312250637988
19	13.040188012124931	19.761873730855093	46.59522318031422	20.602715076705753
20	13.637605788351825	23.817251541892816	43.14609197963482	19.399050690120532
21	12.66685445456724	25.646501889790922	43.204530453171444	18.482113202470394
22	10.446612880848576	23.000374174398903	40.43942368734155	26.113589257410965
23	11.174781486355247	23.819774065930368	37.96356634448429	27.041878103230093
24	13.024632447226695	22.708602227388724	37.705007630635215	26.561757694749367
25	12.799707387211646	25.38836359661477	34.321041634259245	27.49088738191434
26	12.629857435349813	26.181276985751943	36.94698915735085	24.2418764215474
27	14.493161857754869	26.341877682809418	38.20152444536003	20.963436014075683
28	11.83694404621264	24.076651097087744	38.00897177716023	26.077433079539386
29	15.331480679567978	22.08890215549679	35.54152284776147	27.038094317173766
30	21.160613309677665	24.46680148156245	32.31647586575127	22.056109343008615
31	18.881092421076527	31.98728647885074	25.684760171027133	23.446860929045606
32	16.40733718158389	32.024283498068165	24.47058526761878	27.097794052729164
33	17.643794380657283	27.391668103103967	24.497492190686003	30.46704532555275
34	18.85965096675734	23.398512551659188	26.078694341558162	31.663142140025307
35	21.655448441710774	24.368423044097927	23.20133525605721	30.774793258134086
36	21.237550292823	32.56452406277722	22.52277628995573	23.675149354444056
37	15.83052001833034	37.81011279886655	23.527581698247264	22.831785484555848
38	14.403612254421775	37.11515742652098	22.733827467764243	25.747402851293007
39	17.876707433457916	27.67503163665564	23.133647527716235	31.31461340217021
40	19.858990906300843	19.513405113156225	24.604279041609033	36.0233249389339
41	17.082112361629047	23.435929991549546	21.978751939190357	37.503205707631054
42	18.777668935536898	30.144582669419023	24.21833286386358	26.859415531180495
43	17.605956520094008	30.15635444826093	30.371609832798697	21.866079198846368
44	17.738389032065484	25.01503003905708	28.467945025792808	28.778635903084627
45	21.93292608584149	19.14007155559853	26.255691444859725	32.671310913700246
46	23.48511921028181	19.30613772140404	29.947405373817045	27.26133769449711
47	14.962771749412463	25.720495928225784	33.70638661044241	25.610345711919347
48	14.528056773607673	25.047822851545256	34.52410481928217	25.9000155555649
49	20.952505076579627	18.715026255271024	35.74248392941978	24.589984738729573
50	20.885237768911573	14.26277132899179	32.80206174298003	32.049929159116616
51	17.72913977726113	13.493401497538438	29.636714496525222	39.14074422867521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	525.0
1	892.0
2	1259.0
3	6349.5
4	11440.0
5	7183.5
6	2927.0
7	2629.0
8	2331.0
9	2313.5
10	2296.0
11	2169.0
12	2042.0
13	1900.5
14	1759.0
15	1677.5
16	1596.0
17	1449.5
18	1303.0
19	1223.5
20	1144.0
21	1044.0
22	944.0
23	894.5
24	845.0
25	863.0
26	923.5
27	966.0
28	1010.0
29	1054.0
30	1116.0
31	1178.0
32	1356.5
33	1535.0
34	1701.5
35	1868.0
36	2246.5
37	2625.0
38	2962.5
39	3300.0
40	4480.5
41	5661.0
42	7407.5
43	9154.0
44	11595.5
45	14037.0
46	19916.5
47	25796.0
48	32641.0
49	39486.0
50	39414.5
51	39343.0
52	32226.5
53	25110.0
54	18632.0
55	12154.0
56	9150.5
57	6147.0
58	5040.0
59	3933.0
60	3608.0
61	3283.0
62	2968.0
63	2653.0
64	2445.5
65	2238.0
66	1932.5
67	1627.0
68	1388.0
69	1149.0
70	942.0
71	735.0
72	663.5
73	592.0
74	500.5
75	350.0
76	291.0
77	215.0
78	139.0
79	100.0
80	61.0
81	40.5
82	20.0
83	15.0
84	10.0
85	6.5
86	3.0
87	2.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	237857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.616480505199306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54406121236406	32.17971793051391
2	14.113333088781566	12.876011674155771
3	6.218952997028236	8.51060244455068
4	3.082830436661653	5.625114980592454
5	1.6979599793116786	3.8727479147439903
6	0.9865508084531202	2.7001785432714223
7	0.6344715272191026	2.0259650636745956
8	0.4753530237513948	1.7347145552834435
9	0.3361278348378586	1.3799671942622425
>10	1.7194226303260525	14.012423885289685
>50	0.12231867049805854	3.7733607813413976
>100	0.061656565616907556	4.7967680814984295
>500	9.944607357565735E-4	0.29168922606426045
>1k	0.0049723036787828676	2.9459250918527333
>5k	9.944607357565735E-4	3.2748126329049714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7219	3.0350168378479503	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	1602	0.6735139180263772	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1499	0.6302105887150683	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC	1236	0.5196399517357068	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1085	0.456156430123982	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1072	0.45069096137595277	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	643	0.2703304926909866	No Hit
TCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	455	0.19129140618102475	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	352	0.14798807686971585	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	334	0.14042050475705992	No Hit
GCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	325	0.13663671870073196	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	306	0.1286487259151507	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	304	0.12780788456930003	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	302	0.12696704322344937	No Hit
TGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	300	0.12612620187759874	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	286	0.12024031245664413	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	279	0.11729736774616681	No Hit
GTGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	262	0.11015021630643622	No Hit
GGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	250	0.10510516823133227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.204206729253291E-4	0.0	0.0	0.7327932329088486	0.0
2	4.204206729253291E-4	0.0	0.0	2.423725179414522	0.0
3	4.204206729253291E-4	0.0	0.0	3.413815864153672	0.0
4	4.204206729253291E-4	0.0	0.0	4.773876741067112	0.0
5	4.204206729253291E-4	0.0	0.0	7.843788494767865	0.0
6	4.204206729253291E-4	0.0	0.0	10.386492724620256	0.0
7	4.204206729253291E-4	0.0	0.0	12.67231992331527	0.0
8	8.408413458506582E-4	0.0	0.0	16.099589249002552	0.0
9	8.408413458506582E-4	0.0	0.0	17.71904968111092	0.0
10	8.408413458506582E-4	0.0	0.0	21.123616290460234	0.0
11	8.408413458506582E-4	0.0	0.0	29.157014508717424	0.0
12	8.408413458506582E-4	0.0	0.0	36.58458653728921	0.0
13	8.408413458506582E-4	0.0	0.0	39.132335815216706	0.0
14	8.408413458506582E-4	0.0	0.0	40.06987391584019	0.0
15	8.408413458506582E-4	0.0	0.0	41.350054864897814	0.0
16	8.408413458506582E-4	0.0	0.0	44.30687345758166	0.0
17	8.408413458506582E-4	0.0	0.0	47.86279150918409	0.0
18	0.0012612620187759872	0.0	0.0	51.16057126761037	0.0
19	0.0012612620187759872	0.0	0.0	53.05120303375558	0.0
20	0.0012612620187759872	0.0	0.0	54.65258537692815	0.0
21	0.0016816826917013163	0.0	0.0	56.38303686668881	0.0
22	0.0016816826917013163	0.0	0.0	57.81541009934541	0.0
23	0.0016816826917013163	0.0	0.0	58.91985520712024	0.0
24	0.0016816826917013163	0.0	0.0	59.52357929344102	0.0
25	0.0016816826917013163	0.0	0.0	59.90153747840089	0.0
26	0.0016816826917013163	0.0	0.0	60.16387997830629	0.0
27	0.0016816826917013163	0.0	0.0	60.41739364408026	0.0
28	0.0016816826917013163	0.0	0.0	60.62676313919708	0.0
29	0.0016816826917013163	0.0	0.0	60.828565062201235	0.0
30	0.0016816826917013163	0.0	0.0	61.01691352367179	0.0
31	0.0016816826917013163	0.0	0.0	61.192228944281645	0.0
32	0.0016816826917013163	0.0	0.0	61.33433113173041	0.0
33	0.0016816826917013163	0.0	0.0	61.488205098021076	0.0
34	0.0016816826917013163	0.0	0.0	61.63240938883447	0.0
35	0.0016816826917013163	0.0	0.0	61.77198905224568	0.0
36	0.0016816826917013163	0.0	0.0	61.918715867096616	0.0
37	0.0016816826917013163	0.0	0.0	62.055773006470275	0.0
38	0.0016816826917013163	0.0	0.0	62.19997729728366	0.0
39	0.0016816826917013163	0.0	0.0	62.36015757366821	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCT	20	7.0219947E-4	45.000004	33
GATCCTA	20	7.0219947E-4	45.000004	1
GGGTACC	35	1.2064993E-7	45.000004	7
GGCCGAT	20	7.0219947E-4	45.000004	8
TTCGTTG	35	1.2064993E-7	45.000004	1
TAACGTC	20	7.0219947E-4	45.000004	32
TTCCGAT	20	7.0219947E-4	45.000004	13
GCGTTTG	30	2.15822E-6	45.000004	1
TACGGGA	20	7.0219947E-4	45.000004	4
TGTTCGG	30	2.15822E-6	45.000004	2
CTTTGCG	40	6.7757355E-9	45.000004	1
ATTGGGT	20	7.0219947E-4	45.000004	4
ATTGGGC	40	6.7757355E-9	45.000004	4
CGTCGGG	20	7.0219947E-4	45.000004	3
TTACGGG	30	2.15822E-6	45.000004	3
ATAACGT	20	7.0219947E-4	45.000004	31
TGGGCGC	35	1.2064993E-7	45.000004	6
GTTTGCG	25	3.88145E-5	45.0	1
TTGCTCG	25	3.88145E-5	45.0	1
TACGAAT	25	3.88145E-5	45.0	12
>>END_MODULE