##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933624.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104021
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.950087001663125	33.0	31.0	34.0	30.0	34.0
2	32.15923707712866	34.0	31.0	34.0	30.0	34.0
3	32.2350679189779	33.0	31.0	34.0	30.0	34.0
4	35.857884465636744	37.0	35.0	37.0	35.0	37.0
5	35.89270435777391	37.0	35.0	37.0	35.0	37.0
6	35.868055488795534	37.0	35.0	37.0	35.0	37.0
7	35.921736956960615	37.0	35.0	37.0	35.0	37.0
8	35.70973168879361	37.0	35.0	37.0	35.0	37.0
9	37.52789340613914	39.0	37.0	39.0	35.0	39.0
10	37.065842474115804	39.0	37.0	39.0	33.0	39.0
11	37.10994895261534	39.0	37.0	39.0	33.0	39.0
12	37.231193701271856	39.0	37.0	39.0	34.0	39.0
13	37.159265917458974	39.0	37.0	39.0	33.0	39.0
14	38.20859249574605	40.0	38.0	41.0	33.0	41.0
15	38.24616183270686	40.0	37.0	41.0	33.0	41.0
16	38.32680900971919	40.0	38.0	41.0	33.0	41.0
17	38.32775112717624	40.0	38.0	41.0	33.0	41.0
18	37.93344613106969	39.0	37.0	40.0	34.0	41.0
19	37.49506349679392	38.0	37.0	40.0	34.0	41.0
20	36.897203449303504	38.0	35.0	40.0	33.0	41.0
21	36.74022553138309	38.0	35.0	40.0	33.0	41.0
22	36.78099614500918	38.0	35.0	40.0	33.0	41.0
23	36.711298680074215	38.0	35.0	40.0	33.0	41.0
24	36.59141904038608	38.0	35.0	40.0	33.0	41.0
25	36.50152373078513	38.0	35.0	40.0	32.0	41.0
26	36.48858403591583	38.0	35.0	40.0	32.0	41.0
27	36.36434950634968	38.0	35.0	40.0	32.0	41.0
28	36.27765547341402	38.0	35.0	40.0	32.0	41.0
29	36.18676036569539	38.0	35.0	40.0	31.0	41.0
30	36.0017111929322	38.0	35.0	40.0	31.0	41.0
31	35.548523855759896	37.0	35.0	40.0	30.0	41.0
32	35.010324838253815	37.0	35.0	40.0	25.0	41.0
33	34.42029974716644	38.0	34.0	40.0	21.0	41.0
34	33.970429047980694	38.0	34.0	40.0	18.0	41.0
35	33.64532161775026	38.0	34.0	40.0	15.0	41.0
36	33.39686217206141	38.0	33.0	40.0	12.0	41.0
37	33.1674661847127	37.0	33.0	40.0	10.0	41.0
38	33.07969544611184	37.0	33.0	40.0	10.0	41.0
39	32.92370771286567	37.0	33.0	40.0	10.0	41.0
40	32.671268301592946	37.0	32.0	40.0	10.0	41.0
41	32.43811345785947	37.0	31.0	40.0	10.0	41.0
42	32.36133088510974	37.0	31.0	40.0	10.0	41.0
43	32.275232885667315	37.0	31.0	40.0	10.0	41.0
44	32.23327020505475	37.0	31.0	40.0	9.0	41.0
45	32.242143413349226	37.0	31.0	40.0	10.0	41.0
46	31.96356504936503	36.0	30.0	40.0	9.0	41.0
47	31.69999327058959	36.0	30.0	40.0	9.0	41.0
48	31.67371972966997	36.0	30.0	40.0	8.0	41.0
49	31.79523365474279	36.0	30.0	40.0	8.0	41.0
50	31.732755885830745	36.0	30.0	40.0	8.0	41.0
51	30.71484604070332	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	2.0
13	2.0
14	6.0
15	6.0
16	13.0
17	16.0
18	46.0
19	84.0
20	164.0
21	246.0
22	425.0
23	651.0
24	1236.0
25	2114.0
26	3377.0
27	3678.0
28	3314.0
29	3040.0
30	2895.0
31	3078.0
32	3378.0
33	4147.0
34	5824.0
35	8738.0
36	11169.0
37	12892.0
38	20373.0
39	13098.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.69890695148095	5.228751886638275	37.62509493275396	37.44724622912681
2	42.36932927005124	4.811528441372415	36.04656752002	16.77257476855635
3	17.377260360888666	4.955730092961998	60.703127253150804	16.963882292998527
4	13.915459378394749	4.704819219196124	61.19533555724325	20.18438584516588
5	10.998740638909451	6.125686159525481	61.66062621970564	21.21494698185943
6	15.163284336816604	6.7669028369271595	65.73095817190759	12.33885465434864
7	57.11058343988233	2.449505388335048	35.15347862450851	5.286432547274108
8	54.84565616558194	3.2839522788667677	34.8794954864883	6.990896069062978
9	50.20332432874132	4.991299833687428	37.3145807096644	7.490795127906864
10	25.257399948087407	16.86390248122975	42.448159506253546	15.430538064429298
11	15.198854077542034	14.005825746724218	47.26064929196989	23.534670883763855
12	13.736649330423663	11.271762432585728	54.50053354611089	20.491054690879725
13	17.945414868151623	13.404985531767624	55.32056027148364	13.329039328597112
14	15.654531296565116	15.758356485709616	52.989300237452056	15.597811980273214
15	9.664394689533843	14.92775497255362	55.904096288249484	19.50375404966305
16	9.86819969044712	14.61627940512012	52.128897049634205	23.38662385479855
17	10.168139125753454	14.466309687466955	49.79186894953904	25.573682237240558
18	11.08045490814355	13.786639236308055	53.62474884879015	21.50815700675825
19	11.784158967900712	15.624729621903269	54.574557060593534	18.016554349602483
20	11.667836302285115	19.996923698099422	51.30887032426145	17.026369675354015
21	11.220811182357409	21.49181415291143	51.370396362273006	15.916978302458157
22	9.165456975033887	19.771969121619673	48.82091116216918	22.24166274117726
23	9.574028321204372	20.30551523250113	46.39543938243239	23.725017063862104
24	11.638034627623268	19.111525557339384	45.82151680910586	23.428923005931495
25	9.97106353524769	21.52449986060507	43.65464665788639	24.84978994626085
26	9.316388037030984	21.883081300891167	46.07723440459138	22.72329625748647
27	11.400582574672422	20.86501764066871	49.194874111958164	18.539525672700705
28	9.279856951961623	16.94657809480778	50.07738821968641	23.69617673354419
29	12.898357062516222	15.10271964314898	46.92994683765778	25.068976456677017
30	18.51453071975851	16.591842031897404	46.185866315455534	18.70776093288855
31	16.324588304284713	22.159948471943167	41.54737985599062	19.968083367781507
32	15.170013747224118	22.00901740994607	38.31726286038396	24.503705982445855
33	19.104796146931868	17.754107343709443	38.84023418348218	24.300862325876505
34	18.12807029349843	15.181549879351286	39.73140039030581	26.958979436844484
35	16.835062150911835	15.306524644062256	36.3455456109824	31.51286759404351
36	16.574537833706653	18.554907182203593	41.69446554061199	23.17608944347776
37	16.19769085088588	18.221320694859692	47.17701233404794	18.403976120206497
38	22.783860951154093	18.822160909816287	38.63066111650532	19.763317022524298
39	28.649022793474394	17.445515809307736	35.16309206794782	18.74236932927005
40	23.686563290104885	15.238269195643186	39.349746685765375	21.725420828486556
41	15.585314503802117	18.21747531748397	38.0826948404649	28.114515338249007
42	15.462262427779006	21.831168706318916	37.82313186760366	24.883436998298418
43	17.686813239634304	20.37761605829592	41.546418511646685	20.389152190423086
44	19.931552282712143	15.850645542726948	39.84195498985782	24.37584718470309
45	24.451793387873604	12.678209207756124	37.43090337527999	25.43909402909028
46	24.488324472942963	14.075042539487221	41.648321012103324	19.788311975466492
47	15.78335143865181	19.771969121619673	44.61406831312908	19.830611126599436
48	15.488218725065131	19.87579431076417	43.93439786197018	20.70158910220052
49	20.962113419405696	14.73933148114323	44.48332548235452	19.81522961709655
50	20.630449620749655	12.153315195970045	40.170734755482066	27.045500427798235
51	17.22536795454764	12.451331942588515	36.895434575710674	33.42786552715317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	206.0
1	381.0
2	556.0
3	5564.5
4	10573.0
5	6448.5
6	2324.0
7	2196.5
8	2069.0
9	2014.0
10	1959.0
11	1835.5
12	1712.0
13	1624.5
14	1537.0
15	1413.5
16	1290.0
17	1172.5
18	1055.0
19	921.0
20	787.0
21	729.0
22	671.0
23	677.5
24	684.0
25	628.0
26	562.5
27	553.0
28	522.5
29	492.0
30	489.5
31	487.0
32	501.0
33	515.0
34	565.5
35	616.0
36	803.5
37	991.0
38	1157.0
39	1323.0
40	1796.0
41	2269.0
42	3062.0
43	3855.0
44	4712.5
45	5570.0
46	7634.5
47	9699.0
48	12237.5
49	14776.0
50	14507.0
51	14238.0
52	11601.5
53	8965.0
54	6621.5
55	4278.0
56	3293.5
57	2309.0
58	1920.5
59	1532.0
60	1431.0
61	1330.0
62	1267.0
63	1204.0
64	1117.5
65	1031.0
66	889.5
67	748.0
68	603.5
69	459.0
70	379.0
71	299.0
72	255.5
73	212.0
74	173.5
75	104.5
76	74.0
77	53.0
78	32.0
79	26.5
80	21.0
81	14.5
82	8.0
83	5.5
84	3.0
85	2.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	104021.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.47437536651253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58937700372584	43.59696599725055
2	11.267654449354476	12.501321848472905
3	4.311584784680703	7.175474183097644
4	2.141928775669353	4.752886436392652
5	1.119487046183173	3.1051422308956846
6	0.5857378043497097	1.9496063294911603
7	0.3864483147040984	1.5006585208755925
8	0.29806775842647953	1.3228098172484402
9	0.19755653756173644	0.9863392968727468
>10	1.0068451607313058	10.063352592265023
>50	0.06585217918724548	2.4197037136732007
>100	0.024261329174248333	2.7311792811066997
>500	0.0034659041677497617	1.1632266561559685
>1k	0.0	0.0
>5k	0.0017329520838748808	6.731333096201729
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7002	6.731333096201729	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	697	0.6700570077195951	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	513	0.4931696484363734	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	470	0.4518318416473596	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	415	0.39895790273117926	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	387	0.3720402611011238	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	332	0.3191663221849434	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	149	0.14324030724565232	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	147	0.1413176185577912	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	134	0.12882014208669404	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	129	0.12401342036704127	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126	0.12112938733524962	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	118	0.11343863258380518	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	118	0.11343863258380518	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	107	0.10286384480056912	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	106	0.10190250045663857	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48259486065313734	0.0
2	0.0	0.0	0.0	1.8611626498495497	0.0
3	0.0	0.0	0.0	2.653310389248325	0.0
4	0.0	0.0	0.0	3.752126974360946	0.0
5	0.0	0.0	0.0	6.340065948221993	0.0
6	0.0	0.0	0.0	8.231991617077322	0.0
7	0.0	0.0	0.0	10.168139125753454	0.0
8	0.0	0.0	0.0	12.850289845319695	0.0
9	0.0	0.0	0.0	13.951990463464108	0.0
10	0.0	0.0	0.0	16.82544870747253	0.0
11	0.0	0.0	0.0	24.01149767835341	0.0
12	0.0	0.0	0.0	30.680343392199653	0.0
13	0.0	0.0	0.0	32.74146566558676	0.0
14	0.0	0.0	0.0	33.48746887647687	0.0
15	0.0	0.0	0.0	34.48630564982071	0.0
16	0.0	0.0	0.0	36.95407658069044	0.0
17	0.0	0.0	0.0	39.985195297103466	0.0
18	0.0	0.0	0.0	42.825967833418254	0.0
19	0.0	0.0	0.0	44.51601119004816	0.0
20	0.0	0.0	0.0	46.00705626748445	0.0
21	0.0	0.0	0.0	47.50194672229646	0.0
22	0.0	0.0	0.0	48.79110948750733	0.0
23	0.0	0.0	0.0	49.83512944501591	0.0
24	0.0	0.0	0.0	50.36194614548985	0.0
25	0.0	0.0	0.0	50.68880322242624	0.0
26	0.0	0.0	0.0	50.93106199709674	0.0
27	0.0	0.0	0.0	51.167552705703656	0.0
28	0.0	0.0	0.0	51.377125772680515	0.0
29	0.0	0.0	0.0	51.5559358206516	0.0
30	0.0	0.0	0.0	51.73859124599841	0.0
31	0.0	0.0	0.0	51.88567693061978	0.0
32	0.0	0.0	0.0	52.02699454917757	0.0
33	0.0	0.0	0.0	52.16927351207929	0.0
34	0.0	0.0	0.0	52.30290037588564	0.0
35	0.0	0.0	0.0	52.42595245190875	0.0
36	0.0	0.0	0.0	52.54515915055614	0.0
37	0.0	0.0	0.0	52.662443160515664	0.0
38	0.0	0.0	0.0	52.797992713009876	0.0
39	0.0	0.0	0.0	52.94603974197518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGACA	30	2.1464439E-6	45.000004	8
GGCCGAT	35	1.1977318E-7	45.000004	8
GGGAGAC	35	1.1977318E-7	45.000004	7
GCATGGG	30	2.1464439E-6	45.000004	3
GTGCTTG	35	1.1977318E-7	45.000004	1
GGGCCGA	35	1.1977318E-7	45.000004	7
CTCTGCG	20	7.003698E-4	45.0	1
TTGCATG	20	7.003698E-4	45.0	1
AGCTTGG	20	7.003698E-4	45.0	2
CTGGGTG	25	3.8663013E-5	45.0	5
GGACCGA	20	7.003698E-4	45.0	8
TATGTGG	20	7.003698E-4	45.0	2
AGGGCGA	20	7.003698E-4	45.0	6
AACCCGG	25	3.8663013E-5	45.0	39
AGGGATG	40	6.712071E-9	45.0	6
AGGGAAT	20	7.003698E-4	45.0	6
CGGCGTT	25	3.8663013E-5	45.0	43
TAACCCG	25	3.8663013E-5	45.0	38
GACCGAT	20	7.003698E-4	45.0	9
TCTTGGA	25	3.8663013E-5	45.0	3
>>END_MODULE