##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933623.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104917 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86446429082036 33.0 31.0 34.0 30.0 34.0 2 32.090204637951906 33.0 31.0 34.0 30.0 34.0 3 32.18246804616983 33.0 31.0 34.0 30.0 34.0 4 35.768540846573956 37.0 35.0 37.0 35.0 37.0 5 35.86340631165588 37.0 35.0 37.0 35.0 37.0 6 35.87198452109763 37.0 35.0 37.0 35.0 37.0 7 35.850653373619146 37.0 35.0 37.0 35.0 37.0 8 35.67844105340412 37.0 35.0 37.0 35.0 37.0 9 37.501110401555515 39.0 37.0 39.0 35.0 39.0 10 37.10239522670301 39.0 37.0 39.0 33.0 39.0 11 37.131732702993794 39.0 37.0 39.0 33.0 39.0 12 37.20329403242563 39.0 37.0 39.0 34.0 39.0 13 37.16505428100308 39.0 37.0 39.0 33.0 39.0 14 38.26848842418293 40.0 38.0 41.0 33.0 41.0 15 38.264923701592686 40.0 38.0 41.0 33.0 41.0 16 38.42021788651982 40.0 38.0 41.0 34.0 41.0 17 38.475852340421476 40.0 38.0 41.0 34.0 41.0 18 38.12921642822422 39.0 38.0 40.0 34.0 41.0 19 37.72903342642279 38.0 37.0 40.0 34.0 41.0 20 37.10060333406407 38.0 35.0 40.0 34.0 41.0 21 37.004308167408524 38.0 35.0 40.0 33.0 41.0 22 36.99532964152616 38.0 35.0 40.0 33.0 41.0 23 36.87819895727099 38.0 35.0 40.0 33.0 41.0 24 36.76428033588456 38.0 35.0 40.0 33.0 41.0 25 36.699219382940804 38.0 35.0 40.0 33.0 41.0 26 36.71288732998465 38.0 35.0 40.0 33.0 41.0 27 36.628906659549926 38.0 35.0 40.0 33.0 41.0 28 36.60481142236244 38.0 35.0 40.0 33.0 41.0 29 36.452414765957855 38.0 35.0 40.0 32.0 41.0 30 36.15007100851149 38.0 35.0 40.0 31.0 41.0 31 35.684970023923675 38.0 35.0 40.0 30.0 41.0 32 35.24780540808449 38.0 35.0 40.0 27.0 41.0 33 34.567829808324674 38.0 35.0 40.0 21.0 41.0 34 34.11736896785078 38.0 34.0 40.0 18.0 41.0 35 33.81674085229277 38.0 34.0 40.0 15.0 41.0 36 33.56588541418454 38.0 33.0 40.0 14.0 41.0 37 33.36452624455522 38.0 33.0 40.0 10.0 41.0 38 33.241305031596404 38.0 33.0 40.0 10.0 41.0 39 33.153921671416455 38.0 33.0 40.0 10.0 41.0 40 32.875892372065536 37.0 33.0 40.0 10.0 41.0 41 32.69232822135593 37.0 32.0 40.0 10.0 41.0 42 32.56040489148565 37.0 32.0 40.0 10.0 41.0 43 32.48995872928124 37.0 31.0 40.0 10.0 41.0 44 32.41952209842066 37.0 31.0 40.0 10.0 41.0 45 32.39945861967079 37.0 31.0 40.0 10.0 41.0 46 32.099459572805166 37.0 31.0 40.0 10.0 41.0 47 31.975618822497783 36.0 30.0 40.0 9.0 41.0 48 31.902132161613466 36.0 30.0 40.0 9.0 41.0 49 31.991345539807657 36.0 30.0 40.0 8.0 41.0 50 31.85144447515655 36.0 30.0 40.0 8.0 41.0 51 30.983834840874216 35.0 28.0 39.0 8.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 4.0 11 5.0 12 5.0 13 3.0 14 1.0 15 6.0 16 9.0 17 20.0 18 41.0 19 89.0 20 146.0 21 223.0 22 360.0 23 593.0 24 1127.0 25 2265.0 26 3277.0 27 3759.0 28 3413.0 29 2901.0 30 2833.0 31 3098.0 32 3291.0 33 4019.0 34 5656.0 35 8310.0 36 10857.0 37 11753.0 38 20591.0 39 16255.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.818523213587884 5.285130150499919 37.44388421323522 37.45246242267697 2 42.1171020902237 4.873376097295957 35.95127577037086 17.058246042109477 3 17.660626971796752 5.021111926570527 60.34293775079348 16.975323350839236 4 14.241733942068494 4.80856295929163 60.82808315144352 20.121619947196358 5 11.068749583003708 6.294499461479074 61.165492722818996 21.47125823269823 6 15.360713707025553 6.75772277133353 65.5184574473155 12.363106074325419 7 57.2328602609682 2.4409771533688533 34.99814138795429 5.328021197708665 8 55.03207297196832 3.3292983977811033 34.49393329965592 7.1446953305946606 9 50.12724344005262 5.094503274016604 37.343805103081486 7.4344481828493 10 25.469656966935766 16.841884537300913 42.18477463137528 15.50368386438804 11 15.466511623473794 14.300828273778322 47.06577580373057 23.16688429901732 12 13.916715117664438 11.455722142264838 53.966468732426584 20.661094007644138 13 17.98278639305356 13.265724334473916 55.23509059542305 13.516398677049477 14 15.777233432141596 15.914484783209584 52.809363592172865 15.498918192475958 15 10.046036390670722 14.902256069083181 55.52007777576561 19.531629764480492 16 9.863034589246737 14.359922605488148 52.17743549663068 23.599607308634443 17 10.170896994767292 14.527674256793466 49.7011923711124 25.60023637732684 18 11.044921223443293 13.737525853770121 53.54518333539846 21.672369587388125 19 11.790272310493057 15.808686866761345 54.52977115243478 17.871269670310816 20 11.809334998141388 19.906211576770207 51.249082608156925 17.03537081693148 21 11.352783628963847 21.536071370702555 50.94979841207812 16.16134658825548 22 9.103386486460726 19.808991869763716 48.924387849442894 22.163233794332662 23 9.83444055777424 20.498108028250904 45.958233651362505 23.70921776261235 24 11.769303354079891 19.491598120419 45.61224587054529 23.126852654955822 25 10.072724153378385 21.62947854017938 43.387630221985 24.91016708445724 26 9.334045007005539 22.004060352469097 45.85052946614943 22.811365174375936 27 11.474784829913169 20.943221784839444 48.98157591238789 18.600417472859498 28 9.53706263046027 17.31654545974437 49.5801443045455 23.566247605249867 29 12.853970281269955 15.281603553284977 46.75600712944518 25.108419035999884 30 18.456494181114596 16.84569707483058 45.87435782570985 18.823450918344978 31 16.858087821801995 22.104139462622836 41.0991545698028 19.93861814577237 32 15.394073410410133 22.11176453768217 38.08343738383675 24.41072466807095 33 19.094141082951285 17.90653564246023 38.607661294165865 24.39166198042262 34 18.120037744121547 15.235853102928981 39.65324971167685 26.990859441272626 35 16.9848546946634 15.252056387430063 36.35159221098583 31.411496706920712 36 17.000104844782065 18.242038945070867 41.522346235595755 23.235509974551313 37 16.342442120914626 18.386915371198185 47.00668147202074 18.263961035866448 38 22.8437717433781 18.607089413536414 38.86977324933042 19.679365593755062 39 28.622625503969807 17.565313533555095 34.880905858917046 18.93115510355805 40 23.957032702040664 15.459839682796877 39.30249625894755 21.28063135621491 41 15.610434915218697 18.178178941448955 38.2245012724344 27.98688487089795 42 15.539902970919869 21.937340945699933 37.68026154007453 24.84249454330566 43 17.757846678803244 20.33226264571042 41.53283071380234 20.377059961683997 44 20.038697255926113 16.211862710523555 39.662781055501014 24.086658978049318 45 24.381177502216037 13.046503426518106 37.24181972416291 25.330499347102947 46 24.468865865398364 14.327516036485985 41.554752804597925 19.648865293517733 47 16.00217314639191 19.912883517447124 44.23115415042367 19.8537891857373 48 15.432198785706797 20.186433085200683 43.701211433800054 20.68015669529247 49 20.95275312866361 15.151977277276323 43.953792045140446 19.94147754891962 50 20.592468332110144 12.532763994395571 39.986846745522655 26.88792092797164 51 17.281279487594954 12.742453558527217 37.11409971691909 32.86216723695874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 184.0 1 365.0 2 546.0 3 5540.5 4 10535.0 5 6439.5 6 2344.0 7 2226.5 8 2109.0 9 2045.0 10 1981.0 11 1883.0 12 1785.0 13 1655.5 14 1526.0 15 1398.5 16 1271.0 17 1151.0 18 1031.0 19 930.5 20 830.0 21 739.5 22 649.0 23 626.5 24 604.0 25 583.5 26 558.0 27 553.0 28 535.0 29 517.0 30 504.5 31 492.0 32 526.5 33 561.0 34 622.0 35 683.0 36 878.5 37 1074.0 38 1177.0 39 1280.0 40 1802.5 41 2325.0 42 3118.5 43 3912.0 44 4754.5 45 5597.0 46 7656.5 47 9716.0 48 12445.5 49 15175.0 50 14663.0 51 14151.0 52 11565.5 53 8980.0 54 6621.0 55 4262.0 56 3273.5 57 2285.0 58 1952.5 59 1620.0 60 1519.5 61 1419.0 62 1296.5 63 1174.0 64 1159.5 65 1145.0 66 978.0 67 811.0 68 617.0 69 423.0 70 364.5 71 306.0 72 259.5 73 213.0 74 176.5 75 110.5 76 81.0 77 59.5 78 38.0 79 27.0 80 16.0 81 10.5 82 5.0 83 4.0 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 104917.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.60681300456552 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68565845631717 43.75458695921538 2 11.143106905949503 12.392653240180334 3 4.389708781131622 7.3229314601065605 4 2.113436519771687 4.700858774078558 5 1.061003410980271 2.9499509135793054 6 0.6290601806619701 2.0988019100813027 7 0.4113745050650486 1.601265762459849 8 0.2673934282922816 1.189511709255888 9 0.19368882946812704 0.9693376669176588 >10 1.0044394165338268 9.900206830160984 >50 0.0719905383863835 2.5982443264675887 >100 0.022282785691023464 2.223662514177874 >500 0.005142181313313107 1.739470247910253 >1k 0.0 0.0 >5k 0.0017140604377710359 6.558517685408466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6881 6.558517685408466 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC 741 0.7062725773706835 No Hit CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 549 0.5232707759467007 No Hit CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT 535 0.5099268945928687 No Hit TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 413 0.3936444999380463 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC 404 0.3850662904962971 No Hit GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 314 0.29928419607880513 No Hit TCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG 151 0.14392329174490312 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.13057941039107104 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG 130 0.12390746971415499 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 122 0.11628239465482237 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 117 0.11151672274273951 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.11056358836032291 No Hit TTCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT 113 0.1077041852130732 No Hit CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG 108 0.10293851330099031 No Hit GGGCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC 105 0.10007911015374056 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5499585386543648 0.0 2 0.0 0.0 0.0 1.8957842866265715 0.0 3 0.0 0.0 0.0 2.6668700020015823 0.0 4 0.0 0.0 0.0 3.803006185842142 0.0 5 0.0 0.0 0.0 6.275436773830743 0.0 6 0.0 0.0 0.0 8.155017775956232 0.0 7 0.0 0.0 0.0 9.945004146134563 0.0 8 0.0 0.0 0.0 12.642374448373475 0.0 9 0.0 0.0 0.0 13.856667651572195 0.0 10 0.0 0.0 0.0 16.70082064870326 0.0 11 0.0 0.0 0.0 23.794046722647426 0.0 12 0.0 0.0 0.0 30.432627696178884 0.0 13 0.0 0.0 0.0 32.57432065346893 0.0 14 0.0 0.0 0.0 33.30728099354728 0.0 15 0.0 0.0 0.0 34.3204628420561 0.0 16 0.0 0.0 0.0 36.763346264189785 0.0 17 0.0 0.0 0.0 39.852454797601915 0.0 18 0.0 0.0 0.0 42.653716747524236 0.0 19 0.0 0.0 0.0 44.331233260577406 0.0 20 0.0 0.0 0.0 45.69993423372761 0.0 21 0.0 0.0 0.0 47.17729252647331 0.0 22 0.0 0.0 0.0 48.43161737373352 0.0 23 0.0 0.0 0.0 49.358063993442435 0.0 24 0.0 0.0 0.0 49.87180342556497 0.0 25 0.0 0.0 0.0 50.213025534470106 0.0 26 0.0 0.0 0.0 50.45893420513358 0.0 27 0.0 0.0 0.0 50.65623302229381 0.0 28 0.0 0.0 0.0 50.8449536300123 0.0 29 0.0 0.0 0.0 51.01937722199453 0.0 30 0.0 0.0 0.0 51.1842694701526 0.0 31 0.0 0.0 0.0 51.33677097133925 0.0 32 0.0 0.0 0.0 51.46258470981824 0.0 33 0.0 0.0 0.0 51.615086211004886 0.0 34 0.0 0.0 0.0 51.721837261835546 0.0 35 0.0 0.0 0.0 51.867666822345285 0.0 36 0.0 0.0 0.0 52.009683845325355 0.0 37 0.0 0.0 0.0 52.12120056806809 0.0 38 0.0 0.0 0.0 52.256545650371244 0.0 39 0.0 0.0 0.0 52.40046894211615 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAC 20 7.0039765E-4 45.000004 5 AGCTCGC 30 2.1466221E-6 45.000004 19 AATTCTA 30 2.1466221E-6 45.000004 34 CTGGGTA 30 2.1466221E-6 45.000004 5 GTGTAGG 20 7.0039765E-4 45.000004 2 TGTTCGG 20 7.0039765E-4 45.000004 2 CTGATGC 20 7.0039765E-4 45.000004 11 CGGGCTT 20 7.0039765E-4 45.000004 6 ATGGAAG 30 2.1466221E-6 45.000004 40 AGCAGGG 20 7.0039765E-4 45.000004 3 CTTGTTG 30 2.1466221E-6 45.000004 1 TGAGGGC 20 7.0039765E-4 45.000004 4 TGGGCTA 20 7.0039765E-4 45.000004 6 TGGGCGC 20 7.0039765E-4 45.000004 6 TGGGTGA 20 7.0039765E-4 45.000004 6 GGGGTAT 20 7.0039765E-4 45.000004 7 GGGCGCG 20 7.0039765E-4 45.000004 7 ATTCTAT 30 2.1466221E-6 45.000004 35 TGGGAGT 35 1.1978591E-7 45.000004 6 TGGGAGG 70 0.0 45.000004 6 >>END_MODULE