##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933622.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485375
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17459490084986	33.0	31.0	34.0	30.0	34.0
2	32.32044707700232	34.0	31.0	34.0	30.0	34.0
3	32.47025495750708	34.0	31.0	34.0	31.0	34.0
4	35.98568323461242	37.0	35.0	37.0	35.0	37.0
5	35.98647025495751	37.0	35.0	37.0	35.0	37.0
6	35.93801699716714	37.0	35.0	37.0	35.0	37.0
7	35.99423538501159	37.0	35.0	37.0	35.0	37.0
8	35.82229822302344	37.0	35.0	37.0	35.0	37.0
9	37.653937677053825	39.0	38.0	39.0	35.0	39.0
10	37.13588462528973	39.0	37.0	39.0	33.0	39.0
11	37.11446613443214	39.0	37.0	39.0	34.0	39.0
12	37.0179098635076	39.0	37.0	39.0	33.0	39.0
13	36.95734638166366	39.0	37.0	39.0	33.0	39.0
14	37.92298532062838	40.0	37.0	41.0	33.0	41.0
15	38.041466907030646	40.0	37.0	41.0	33.0	41.0
16	38.14052639711563	40.0	37.0	41.0	33.0	41.0
17	38.1195755858872	40.0	37.0	41.0	33.0	41.0
18	37.96378882307494	39.0	37.0	41.0	33.0	41.0
19	37.791361318568114	39.0	37.0	41.0	33.0	41.0
20	37.51547978367242	39.0	35.0	41.0	33.0	41.0
21	37.392024723152204	39.0	35.0	41.0	33.0	41.0
22	37.428627349987124	39.0	35.0	41.0	33.0	41.0
23	37.371867113056915	39.0	35.0	41.0	33.0	41.0
24	37.29356889003348	39.0	35.0	41.0	33.0	41.0
25	37.19608756116405	39.0	35.0	41.0	33.0	41.0
26	37.10327478753541	39.0	35.0	40.0	33.0	41.0
27	37.02437908833376	39.0	35.0	40.0	33.0	41.0
28	36.868435745557555	39.0	35.0	40.0	32.0	41.0
29	36.769745042492914	39.0	35.0	40.0	32.0	41.0
30	36.66414627865053	39.0	35.0	40.0	31.0	41.0
31	36.4267834148854	38.0	35.0	40.0	31.0	41.0
32	36.14474375482874	38.0	35.0	40.0	30.0	41.0
33	35.90915889775946	38.0	35.0	40.0	29.0	41.0
34	35.653993304146276	38.0	35.0	40.0	27.0	41.0
35	35.41774298223024	38.0	35.0	40.0	25.0	41.0
36	35.24854184908576	38.0	35.0	40.0	25.0	41.0
37	35.08099304661344	38.0	34.0	40.0	24.0	41.0
38	35.024568632500646	38.0	34.0	40.0	24.0	41.0
39	35.00923203708473	38.0	34.0	40.0	24.0	41.0
40	34.93035075972186	38.0	34.0	40.0	23.0	41.0
41	34.74307288179243	38.0	34.0	40.0	23.0	41.0
42	34.513180530517644	38.0	34.0	40.0	23.0	41.0
43	34.3644810713366	37.0	34.0	40.0	22.0	41.0
44	34.284394540303886	37.0	34.0	40.0	21.0	41.0
45	34.348021632758176	37.0	34.0	40.0	22.0	41.0
46	34.21623899047128	37.0	33.0	40.0	22.0	41.0
47	34.03660674736029	37.0	33.0	40.0	21.0	41.0
48	33.95731547772341	37.0	33.0	40.0	21.0	41.0
49	33.98995003862993	37.0	33.0	40.0	21.0	41.0
50	33.9366489827453	37.0	33.0	40.0	20.0	41.0
51	32.847925830543396	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	9.0
12	3.0
13	11.0
14	14.0
15	27.0
16	58.0
17	78.0
18	141.0
19	275.0
20	496.0
21	753.0
22	1292.0
23	2104.0
24	3432.0
25	5734.0
26	8364.0
27	9693.0
28	9489.0
29	9561.0
30	10341.0
31	12036.0
32	15089.0
33	20872.0
34	33426.0
35	37370.0
36	46810.0
37	64734.0
38	101960.0
39	91177.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.246046870976052	5.38490857584342	39.14293072366727	26.226113829513263
2	32.261241308266804	5.206077774916302	45.11336595415916	17.41931496265774
3	22.67834148853979	5.189389647180016	53.013855266546486	19.11841359773371
4	17.78872006180788	5.742776203966005	53.70095287149111	22.767550862734996
5	16.386299253154775	6.713984032964203	53.25552407932012	23.644192634560905
6	20.323255215039918	7.661704867370591	56.54452742724697	15.47051249034252
7	73.72876641771826	3.0454802987380893	18.60211176925058	4.623641514293072
8	73.29611125418491	3.5702292042235384	17.83013134174607	5.30352819984548
9	67.39654906000516	5.781921194952357	20.555446819469484	6.26608292557301
10	34.33963430337368	22.389286634045842	26.94081895441669	16.33026010816379
11	26.26052021632758	20.741076487252126	32.35683749678084	20.641565799639455
12	22.501983002832862	19.257069276332732	37.78707185166109	20.45387586917332
13	23.844656193664694	19.156116404841615	39.72062838011847	17.278599021375225
14	20.151223280968324	20.431212979654905	40.866340458408445	18.551223280968323
15	18.170074684522277	19.136131856811744	41.87813546227144	20.815657996394542
16	20.148132886943085	19.538089106361063	38.5431882565027	21.77058975019315
17	19.785938707185167	19.87411795003863	37.34617563739377	22.993767705382435
18	21.37398918362091	18.060468709760492	39.028998197270155	21.536543909348442
19	20.766623744527426	21.559824877671904	37.476178212722125	20.197373165078545
20	21.244398660829255	24.803502446561936	35.34957507082153	18.60252382178728
21	20.357455575585888	25.277980942570178	36.182333247489055	18.18223023435488
22	18.69379345866598	22.312644862219933	36.08611898016997	22.907442698944116
23	17.923049188771568	23.911408704609837	35.20432655163533	22.961215554983262
24	18.411537471027557	24.21488539788823	35.06649497810971	22.307082152974505
25	19.541797579191346	25.580427504506826	31.91429307236673	22.963481843935103
26	18.31367499356168	25.184857069276333	33.56250321916045	22.938964718001547
27	19.224311099665208	23.410558846252897	35.29477208344064	22.070357970641258
28	17.03363378830801	24.566984290497036	34.38042750450682	24.018954416688125
29	19.144372907545712	24.234663919649755	33.098120010301315	23.522843162503218
30	24.360751995879472	23.345042492917848	32.546999742467165	19.747205768735515
31	23.455884625289723	27.15302601081638	29.0762812258563	20.3148081380376
32	22.333247489054855	26.750038629925317	28.861395827968067	22.055318053051764
33	25.03239763069791	23.759361318568118	28.537934586659798	22.67030646407417
34	23.653463816636624	24.887767190316765	29.799845480298735	21.658923512747876
35	23.560751995879475	26.830388874581505	28.919083183105847	20.68977594643317
36	27.29642029358743	28.12361576100953	26.928457378315734	17.651506567087303
37	25.630079835178986	27.858253927375742	28.328199845480302	18.183466391964977
38	24.766829770795777	25.892351274787533	30.34787535410765	18.99294360030904
39	26.353644089621426	22.398969868658252	30.274942055112025	20.97244398660829
40	30.380633530775174	22.29204223538501	28.552150399175897	18.77517383466392
41	27.412825135204738	23.742879217100178	27.87638423899047	20.96791140870461
42	25.285397888230747	24.221272212207058	31.448467679629154	19.04486221993304
43	22.440175122328096	22.551841359773373	34.71789853206283	20.290084985835694
44	23.277465876899306	23.225135204738603	31.36461498841102	22.13278392995107
45	25.595879474633016	21.564769508112285	29.04393510172547	23.79541591552923
46	26.444501673963433	22.30852433685295	30.289569920164823	20.9574040690188
47	22.54998712335823	25.1269636878702	32.94380633530775	19.379242853463815
48	21.87525109451455	24.44934329126964	33.79696111254185	19.878444501673965
49	24.528457378315736	21.580633530775174	33.0235385011589	20.86737058975019
50	22.95833118722637	20.973886170486736	31.63842389904713	24.429358743239764
51	22.05160958022148	20.07128508884883	30.138964718001542	27.73814061292815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	679.0
1	1350.0
2	2021.0
3	9954.0
4	17887.0
5	11231.5
6	4576.0
7	4469.0
8	4362.0
9	4360.0
10	4358.0
11	4195.5
12	4033.0
13	3967.0
14	3901.0
15	3668.0
16	3435.0
17	3288.0
18	3141.0
19	2926.5
20	2712.0
21	2740.0
22	2768.0
23	2768.0
24	2768.0
25	3022.5
26	3418.5
27	3560.0
28	3789.5
29	4019.0
30	4514.5
31	5010.0
32	5830.0
33	6650.0
34	7656.0
35	8662.0
36	9072.0
37	9482.0
38	10190.0
39	10898.0
40	12588.5
41	14279.0
42	16516.0
43	18753.0
44	21802.5
45	24852.0
46	31397.5
47	37943.0
48	44654.5
49	51366.0
50	53434.0
51	55502.0
52	49390.0
53	43278.0
54	36725.0
55	30172.0
56	26563.0
57	22954.0
58	20767.0
59	18580.0
60	16939.5
61	15299.0
62	13832.5
63	12366.0
64	10979.5
65	9593.0
66	8241.0
67	6889.0
68	5951.0
69	5013.0
70	4118.5
71	3224.0
72	2870.0
73	2516.0
74	2261.5
75	1667.0
76	1327.0
77	1005.5
78	684.0
79	527.5
80	371.0
81	257.5
82	144.0
83	88.0
84	32.0
85	27.0
86	22.0
87	13.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.12233325664121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29194006620739	30.029828579812794
2	10.207717226401277	8.599457336000638
3	4.912100713556582	6.207274297398464
4	3.1155304515797417	5.2493364781062315
5	2.229667760806543	4.695940423614109
6	1.6278210011350775	4.114057121518265
7	1.2523529466053416	3.6926419720292767
8	1.0041874755372517	3.383897559738029
9	0.7917849202773979	3.001664545155689
>10	3.4423475804563632	22.700553356890154
>50	0.08581190965162838	2.5116291386094494
>100	0.03481511762986907	2.424052596264795
>500	0.0029421226166086536	0.8476748342766462
>1k	4.903537694347757E-4	0.28565650482568267
>5k	0.0	0.0
>10k+	4.903537694347757E-4	2.2563352557597667
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10924	2.2506309554468196	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	1383	0.28493432912696365	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	895	0.184393510172547	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	850	0.17512232809683234	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	687	0.14154004635591036	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	604	0.12443986608292558	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.11372650012876641	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	516	0.10630955446819469	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	500	0.10301313417460728	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0602626834921454E-4	0.0	0.0	0.1913984032964203	0.0
2	2.0602626834921454E-4	0.0	0.0	0.6582539273757404	0.0
3	2.0602626834921454E-4	0.0	0.0	0.9646149884110224	0.0
4	2.0602626834921454E-4	0.0	0.0	1.453927375740407	0.0
5	2.0602626834921454E-4	0.0	0.0	2.685552407932011	0.0
6	2.0602626834921454E-4	0.0	0.0	4.208498583569405	0.0
7	2.0602626834921454E-4	0.0	0.0	5.504197785217615	0.0
8	2.0602626834921454E-4	0.0	0.0	7.3600824105073395	0.0
9	2.0602626834921454E-4	0.0	0.0	8.464177182590781	0.0
10	4.1205253669842907E-4	0.0	0.0	10.434406386814318	0.0
11	4.1205253669842907E-4	0.0	0.0	14.203656966263198	0.0
12	4.1205253669842907E-4	0.0	0.0	17.349266031419006	0.0
13	4.1205253669842907E-4	0.0	0.0	18.55534380633531	0.0
14	4.1205253669842907E-4	0.0	0.0	19.100489312387328	0.0
15	4.1205253669842907E-4	0.0	0.0	19.69384496523307	0.0
16	4.1205253669842907E-4	0.0	0.0	20.97285603914499	0.0
17	6.180788050476436E-4	0.0	0.0	22.495390162245688	0.0
18	6.180788050476436E-4	0.0	0.0	23.929951068761266	0.0
19	6.180788050476436E-4	0.0	0.0	24.787844450167395	0.0
20	6.180788050476436E-4	0.0	0.0	25.561679114087045	0.0
21	6.180788050476436E-4	0.0	0.0	26.432758176667527	0.0
22	6.180788050476436E-4	0.0	0.0	27.199381921194952	0.0
23	6.180788050476436E-4	0.0	0.0	27.777491630182848	0.0
24	6.180788050476436E-4	0.0	0.0	28.16502704094772	0.0
25	6.180788050476436E-4	0.0	0.0	28.445222765902653	0.0
26	6.180788050476436E-4	0.0	0.0	28.69472057687355	0.0
27	6.180788050476436E-4	0.0	0.0	28.953901622456865	0.0
28	8.241050733968581E-4	0.0	0.0	29.189595673448366	0.0
29	8.241050733968581E-4	0.0	0.0	29.43167653875869	0.0
30	8.241050733968581E-4	0.0	0.0	29.735565284573784	0.0
31	8.241050733968581E-4	0.0	0.0	29.979088333762554	0.0
32	8.241050733968581E-4	0.0	0.0	30.406592840587177	0.0
33	8.241050733968581E-4	0.0	0.0	30.640844707700232	0.0
34	8.241050733968581E-4	0.0	0.0	30.905176409992276	0.0
35	8.241050733968581E-4	0.0	0.0	31.187432397630698	0.0
36	8.241050733968581E-4	0.0	0.0	31.4361061035282	0.0
37	8.241050733968581E-4	0.0	0.0	31.652845737831573	0.0
38	8.241050733968581E-4	0.0	0.0	31.982075714653618	0.0
39	8.241050733968581E-4	0.0	0.0	32.63888745815091	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	65	0.0	45.000004	11
CGGGTAT	20	7.0292543E-4	45.0	6
GAATCGC	20	7.0292543E-4	45.0	17
GTCCGAC	20	7.0292543E-4	45.0	25
GCGATAT	20	7.0292543E-4	45.0	9
CCGACGG	20	7.0292543E-4	45.0	27
TTACGGG	35	1.2099918E-7	45.0	3
CCGGTAT	20	7.0292543E-4	45.0	42
CGGTCCG	20	7.0292543E-4	45.0	23
TATCGGG	20	7.0292543E-4	45.0	3
AATCGCG	20	7.0292543E-4	45.0	18
CGTTTTT	4640	0.0	43.3028	1
TCGTTTG	95	0.0	42.63158	1
AACACGT	270	0.0	42.5	41
CGAGACA	360	0.0	41.875004	22
CGACCAA	355	0.0	41.830986	29
GCGAGAC	365	0.0	41.30137	21
ACACGAC	360	0.0	41.250004	26
GACACGA	360	0.0	41.250004	25
CGAATAT	60	3.6379788E-12	41.250004	14
>>END_MODULE