##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933621.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490641
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05626720962985	33.0	31.0	34.0	30.0	34.0
2	32.23518621558329	34.0	31.0	34.0	30.0	34.0
3	32.40520258192854	34.0	31.0	34.0	30.0	34.0
4	35.885455964748154	37.0	35.0	37.0	35.0	37.0
5	35.95328559985815	37.0	35.0	37.0	35.0	37.0
6	35.92749484857564	37.0	35.0	37.0	35.0	37.0
7	35.92002910478333	37.0	35.0	37.0	35.0	37.0
8	35.79759131421956	37.0	35.0	37.0	35.0	37.0
9	37.628060027596554	39.0	38.0	39.0	35.0	39.0
10	37.16673902099498	39.0	37.0	39.0	34.0	39.0
11	37.13517826679792	39.0	37.0	39.0	34.0	39.0
12	37.02038965353486	39.0	37.0	39.0	33.0	39.0
13	36.95589850827795	39.0	37.0	39.0	33.0	39.0
14	37.965314761709685	40.0	37.0	41.0	33.0	41.0
15	38.063072592791876	40.0	37.0	41.0	33.0	41.0
16	38.22297362022334	40.0	37.0	41.0	33.0	41.0
17	38.21963920667046	40.0	37.0	41.0	33.0	41.0
18	38.1284034558873	39.0	37.0	41.0	34.0	41.0
19	37.986607315735945	39.0	37.0	41.0	34.0	41.0
20	37.666240693297134	39.0	35.0	41.0	33.0	41.0
21	37.58626979808047	39.0	35.0	41.0	33.0	41.0
22	37.6010402718077	39.0	35.0	41.0	33.0	41.0
23	37.49013637262275	39.0	35.0	41.0	33.0	41.0
24	37.41352434876009	39.0	35.0	41.0	33.0	41.0
25	37.323523716933565	39.0	35.0	41.0	33.0	41.0
26	37.285773100902695	39.0	35.0	41.0	33.0	41.0
27	37.21017607578657	39.0	35.0	41.0	33.0	41.0
28	37.12665064680693	39.0	35.0	41.0	33.0	41.0
29	36.97640433636814	39.0	35.0	41.0	32.0	41.0
30	36.79186411245697	39.0	35.0	41.0	32.0	41.0
31	36.552312179373516	39.0	35.0	40.0	31.0	41.0
32	36.359570439486305	39.0	35.0	40.0	31.0	41.0
33	36.06005001620329	39.0	35.0	41.0	30.0	41.0
34	35.81054375806343	39.0	35.0	41.0	28.0	41.0
35	35.62972723437299	39.0	35.0	41.0	27.0	41.0
36	35.44052372304801	38.0	35.0	40.0	25.0	41.0
37	35.28313573468177	38.0	35.0	40.0	25.0	41.0
38	35.17807928811494	38.0	35.0	40.0	25.0	41.0
39	35.20935470129891	38.0	35.0	40.0	25.0	41.0
40	35.12170405653013	38.0	35.0	40.0	24.0	41.0
41	34.96731826325154	38.0	35.0	40.0	23.0	41.0
42	34.657635623602594	38.0	34.0	40.0	23.0	41.0
43	34.54448364486458	38.0	34.0	40.0	23.0	41.0
44	34.47401256723347	38.0	34.0	40.0	22.0	41.0
45	34.52000546224225	38.0	34.0	40.0	23.0	41.0
46	34.35994342095341	37.0	34.0	40.0	22.0	41.0
47	34.28929706241427	37.0	34.0	40.0	22.0	41.0
48	34.161411296650705	37.0	33.0	40.0	22.0	41.0
49	34.16472532870266	37.0	34.0	40.0	22.0	41.0
50	34.01696352322778	37.0	33.0	40.0	21.0	41.0
51	33.094743407093986	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	10.0
11	8.0
12	17.0
13	16.0
14	22.0
15	32.0
16	56.0
17	63.0
18	168.0
19	269.0
20	481.0
21	791.0
22	1311.0
23	1990.0
24	3277.0
25	5615.0
26	8231.0
27	9395.0
28	9479.0
29	9445.0
30	10187.0
31	11633.0
32	14567.0
33	20124.0
34	32976.0
35	36647.0
36	45543.0
37	62074.0
38	102847.0
39	103321.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.284140542677843	5.626720962985156	39.03791162988825	26.05122686444875
2	32.33932753275817	5.193206438108515	44.97932296730196	17.48814306183136
3	22.98625675391987	5.247625045603608	52.50295022226027	19.263167978216252
4	17.974038044109644	5.7728563246854625	53.281727372967204	22.971378258237692
5	16.495767781330954	6.784186401054947	53.111745655173536	23.608300162440564
6	20.28040868985674	7.728461339349953	56.49140614013098	15.499723830662337
7	73.67015801777674	3.133451953668772	18.468697071789762	4.727692956764722
8	73.46328578329165	3.5325217419661223	17.68380547080248	5.320387003939745
9	67.51249895544808	5.740042108180931	20.403920585519757	6.343538350851233
10	34.182630477273605	22.506883851940625	26.838156615529478	16.47232905525629
11	26.341051807737227	20.72024148002307	32.312627766533986	20.62607894570572
12	22.48466801592203	19.44537859657061	37.57778090294126	20.492172484566108
13	23.78174673539309	19.291090634496506	39.73312462676377	17.19403800334664
14	20.281427764903462	20.44162636224857	40.777268919637784	18.49967695321019
15	18.274257552874708	19.257868787973283	41.59599381217632	20.87187984697569
16	20.175647775053452	19.532407605560888	38.465191453629025	21.82675316575663
17	19.952470339820767	19.766794866307542	37.340744047073116	22.939990746798575
18	21.35084511893625	18.168885193043387	38.81188078452473	21.668388903495632
19	20.758354886770572	21.734424966523385	37.5099105048294	19.997309641876647
20	21.39221956583327	24.88581264101451	35.16563026734415	18.556337525808075
21	20.55107502226679	25.207228910751446	36.02736012685446	18.214335940127302
22	18.84820061919	22.362175195305735	36.00208706569569	22.787537119808576
23	18.009705670745007	24.001255500457564	35.062296057606275	22.926742771191154
24	18.47949926728504	24.238699986344393	34.9970752546159	22.284725491754664
25	19.675485742121023	25.715339729048324	31.872591161358304	22.736583367472345
26	18.487244237640148	25.17176509912543	33.366555179856555	22.974435483377867
27	19.105415160983284	23.554900629992193	35.26020043168019	22.07948377734433
28	17.15388644650569	24.50447475853017	34.34364433465609	23.997994460308046
29	19.185106829637146	24.360785176942002	32.99275845271797	23.461349540702876
30	24.35732032178314	23.35251232571269	32.524391561243355	19.765775791260822
31	23.579358431113583	27.208488487509197	28.92664901628686	20.28550406509036
32	22.310406182932123	26.765394657193347	28.788666254960347	22.135532904914186
33	25.11286256142475	23.67127900032814	28.52594870791475	22.68990973033236
34	23.703074141785947	24.846680159220284	29.675669175629434	21.774576523364335
35	23.66027298982352	27.000800992986722	28.810474460960258	20.528451556229506
36	27.26698339519119	28.196176022794674	26.938433600127183	17.59840698188696
37	25.521307840151962	28.002551763917	28.190265387523667	18.285875008407366
38	24.633693474454844	26.075480850560794	30.52353961450429	18.767286060480064
39	26.47679260396094	22.438198193791386	30.039478967310114	21.045530234937562
40	30.346424371383556	22.484871830931375	28.52146477770916	18.64723901997591
41	27.428404882592368	23.784803960533264	27.860696517412936	20.92609463946144
42	25.355402422545204	24.33755026587668	31.40361282485565	18.90343448672247
43	22.35626456003473	22.63977123803351	34.75127435334593	20.25268984858583
44	23.46481439586174	23.17478563756392	31.26848347365997	22.091916492914372
45	25.613228409366524	21.642504397308826	28.965373868062393	23.77889332526226
46	26.371827874148305	22.433510448576456	30.335825990897618	20.858835686377617
47	22.49017102117434	25.319938610919184	32.970542616699376	19.219347751207096
48	21.996734883550296	24.626152319109085	33.74626254226614	19.630850255074485
49	24.61514630860446	21.624568676486476	32.974211286867586	20.78607372804148
50	23.04964322182614	21.055924800414154	31.533850615827053	24.360581361932656
51	22.02893765502679	20.328305217052794	30.19988137966456	27.44287574825585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	659.0
1	1301.0
2	1943.0
3	9901.0
4	17859.0
5	11287.0
6	4715.0
7	4555.0
8	4395.0
9	4380.5
10	4366.0
11	4236.5
12	4107.0
13	4046.5
14	3986.0
15	3679.0
16	3372.0
17	3224.0
18	3076.0
19	2963.5
20	2851.0
21	2775.0
22	2699.0
23	2751.5
24	2804.0
25	3022.5
26	3390.0
27	3539.0
28	3840.5
29	4142.0
30	4668.5
31	5195.0
32	5905.5
33	6616.0
34	7791.0
35	8966.0
36	9337.0
37	9708.0
38	10407.5
39	11107.0
40	12958.5
41	14810.0
42	16894.0
43	18978.0
44	21939.0
45	24900.0
46	31432.0
47	37964.0
48	45232.0
49	52500.0
50	53932.0
51	55364.0
52	49500.0
53	43636.0
54	37151.5
55	30667.0
56	26863.5
57	23060.0
58	21144.0
59	19228.0
60	17393.5
61	15559.0
62	14093.0
63	12627.0
64	11219.5
65	9812.0
66	8425.5
67	7039.0
68	5987.5
69	4936.0
70	4054.0
71	3172.0
72	2828.0
73	2484.0
74	2241.5
75	1648.5
76	1298.0
77	1003.0
78	708.0
79	524.5
80	341.0
81	242.0
82	143.0
83	88.5
84	34.0
85	26.5
86	19.0
87	13.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	490641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.17542353142883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4300513790177	30.125926697817935
2	10.136944070725814	8.55059818994535
3	4.895584759064881	6.194200819427078
4	3.0476563802644274	5.141447944636542
5	2.2167371328732384	4.6745913718387015
6	1.6631891935079253	4.208742518945536
7	1.2071412825705914	3.563818639828082
8	0.989977539262635	3.340217760400267
9	0.7944845413537112	3.0156949818689176
>10	3.5003539141064106	23.10257400207945
>50	0.07452731615576035	2.162659396558718
>100	0.038968531323018425	2.4891024269567965
>500	0.003409746490764112	0.9055763864332749
>1k	4.871066415377303E-4	0.2863823690308497
>5k	0.0	0.0
>10k+	4.871066415377303E-4	2.2384664942325236
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10896	2.2207683418222284	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	1394	0.28411812302681594	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	854	0.17405801798056011	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	757	0.15428796207410306	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	698	0.14226287652275288	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	567	0.1155631102985686	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	517	0.1053723598313227	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	510	0.10394565476590828	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.10292657971918368	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18812125362535947	0.0
2	0.0	0.0	0.0	0.6014580925768536	0.0
3	0.0	0.0	0.0	0.8937288159774662	0.0
4	0.0	0.0	0.0	1.367802527713746	0.0
5	0.0	0.0	0.0	2.563585187540381	0.0
6	0.0	0.0	0.0	4.094643537739406	0.0
7	0.0	0.0	0.0	5.373582721378768	0.0
8	0.0	0.0	0.0	7.171434918810291	0.0
9	0.0	0.0	0.0	8.26673677902988	0.0
10	0.0	0.0	0.0	10.19951451264774	0.0
11	0.0	0.0	0.0	13.96234721517362	0.0
12	0.0	0.0	0.0	17.082958823253662	0.0
13	0.0	0.0	0.0	18.240831891342143	0.0
14	0.0	0.0	0.0	18.782979816199624	0.0
15	0.0	0.0	0.0	19.38076923860827	0.0
16	0.0	0.0	0.0	20.644626111556107	0.0
17	0.0	0.0	0.0	22.141443540185186	0.0
18	0.0	0.0	0.0	23.53105427389884	0.0
19	0.0	0.0	0.0	24.36119280696069	0.0
20	0.0	0.0	0.0	25.147918743032076	0.0
21	0.0	0.0	0.0	25.984171726374274	0.0
22	0.0	0.0	0.0	26.73033847558602	0.0
23	0.0	0.0	0.0	27.297351831583583	0.0
24	0.0	0.0	0.0	27.668295148591334	0.0
25	0.0	0.0	0.0	27.944872116272386	0.0
26	0.0	0.0	0.0	28.174367816794764	0.0
27	0.0	0.0	0.0	28.424652648270325	0.0
28	0.0	0.0	0.0	28.65740938894222	0.0
29	0.0	0.0	0.0	28.909936185520575	0.0
30	0.0	0.0	0.0	29.219123554696814	0.0
31	0.0	0.0	0.0	29.457383300621025	0.0
32	0.0	0.0	0.0	29.86949724951645	0.0
33	0.0	0.0	0.0	30.11448289074904	0.0
34	0.0	0.0	0.0	30.35396552672932	0.0
35	0.0	0.0	0.0	30.633803534559892	0.0
36	0.0	0.0	0.0	30.859019119886028	0.0
37	0.0	0.0	0.0	31.089126265436438	0.0
38	0.0	0.0	0.0	31.409319645117307	0.0
39	2.038150093449182E-4	0.0	0.0	32.08924651629195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	20	7.02933E-4	45.0	2
TACTCGA	20	7.02933E-4	45.0	44
CGTTTCG	20	7.02933E-4	45.0	1
ACGAACG	20	7.02933E-4	45.0	21
CGTTTTT	4815	0.0	43.551403	1
GGCGATA	55	6.002665E-11	40.909092	8
GCGCGAC	45	1.9244908E-8	40.0	9
GCTTGCG	90	0.0	40.0	1
GTACGGG	45	1.9244908E-8	40.0	3
AACACGT	255	0.0	39.705883	41
GACCGAT	210	0.0	39.642857	9
GGACCGA	210	0.0	39.642857	8
ACGTGAG	250	0.0	39.600002	44
CGTGGCA	40	3.452824E-7	39.375	15
TCAGACG	40	3.452824E-7	39.375	22
GCGATTC	150	0.0	39.000004	9
CTTGTTG	225	0.0	39.0	1
TGTACGG	35	6.2409727E-6	38.571426	2
CACGACC	365	0.0	38.219177	27
ACACGTG	265	0.0	38.207546	42
>>END_MODULE