##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933620.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87324 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3393797810453 33.0 31.0 34.0 31.0 34.0 2 32.4735238880491 34.0 31.0 34.0 31.0 34.0 3 32.569465439054554 34.0 31.0 34.0 31.0 34.0 4 36.0840662360863 37.0 35.0 37.0 35.0 37.0 5 36.01789885942009 37.0 35.0 37.0 35.0 37.0 6 35.95696486647428 37.0 35.0 37.0 35.0 37.0 7 36.00814209152123 37.0 35.0 37.0 35.0 37.0 8 35.83686042783198 37.0 35.0 37.0 35.0 37.0 9 37.67684714396958 39.0 38.0 39.0 35.0 39.0 10 37.200093903165225 39.0 37.0 39.0 34.0 39.0 11 37.10170170857954 39.0 37.0 39.0 34.0 39.0 12 36.982960010993544 39.0 37.0 39.0 33.0 39.0 13 36.940314232055336 39.0 37.0 39.0 33.0 39.0 14 38.11136686363428 40.0 37.0 41.0 33.0 41.0 15 38.19396729421465 40.0 38.0 41.0 33.0 41.0 16 38.22692501488709 40.0 37.0 41.0 33.0 41.0 17 38.18965003893546 40.0 37.0 41.0 33.0 41.0 18 37.93670697631808 39.0 37.0 41.0 33.0 41.0 19 37.6632769914342 39.0 37.0 41.0 33.0 41.0 20 37.31209060510284 39.0 35.0 41.0 33.0 41.0 21 37.21042325133984 39.0 35.0 41.0 33.0 41.0 22 37.19236406944253 39.0 35.0 41.0 33.0 41.0 23 37.081512528056436 39.0 35.0 40.0 33.0 41.0 24 36.95435390041684 38.0 35.0 41.0 33.0 41.0 25 36.869520406761026 38.0 35.0 40.0 32.0 41.0 26 36.777919014245796 38.0 35.0 40.0 32.0 41.0 27 36.70449131968302 38.0 35.0 40.0 32.0 41.0 28 36.5259493380972 38.0 35.0 40.0 31.0 41.0 29 36.38927442627456 38.0 35.0 40.0 31.0 41.0 30 36.24546516421602 38.0 35.0 40.0 31.0 41.0 31 35.9860404928771 38.0 35.0 40.0 30.0 41.0 32 35.55702899546517 38.0 35.0 40.0 27.0 41.0 33 35.09910219412762 38.0 35.0 40.0 23.0 41.0 34 34.65747102743805 38.0 34.0 40.0 21.0 41.0 35 34.33013833539462 38.0 34.0 40.0 18.0 41.0 36 34.164479410013286 38.0 34.0 40.0 18.0 41.0 37 34.02018918052311 38.0 34.0 40.0 16.0 41.0 38 33.892961843250426 38.0 33.0 40.0 15.0 41.0 39 33.771574824790434 38.0 33.0 40.0 15.0 41.0 40 33.60111767669827 38.0 33.0 40.0 15.0 41.0 41 33.45404470706793 37.0 33.0 40.0 15.0 41.0 42 33.33546333195914 37.0 33.0 40.0 14.0 41.0 43 33.21691631166689 37.0 33.0 40.0 12.0 41.0 44 33.059685767944664 37.0 33.0 40.0 12.0 41.0 45 32.98529613851862 36.0 33.0 40.0 12.0 41.0 46 32.86755073061243 36.0 32.0 40.0 12.0 41.0 47 32.74971370986212 36.0 32.0 40.0 10.0 41.0 48 32.64037378040401 36.0 32.0 40.0 10.0 41.0 49 32.62339104942513 36.0 31.0 40.0 10.0 41.0 50 32.53617562182218 35.0 31.0 40.0 10.0 41.0 51 31.56893866520086 35.0 29.0 39.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 0.0 10 3.0 11 4.0 12 4.0 13 7.0 14 3.0 15 6.0 16 14.0 17 27.0 18 47.0 19 109.0 20 153.0 21 249.0 22 325.0 23 519.0 24 858.0 25 1546.0 26 2220.0 27 2783.0 28 2476.0 29 2300.0 30 2144.0 31 2221.0 32 2627.0 33 3504.0 34 5582.0 35 6137.0 36 7622.0 37 10199.0 38 16110.0 39 17517.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.755256286931427 4.43864229765013 37.68494342906876 30.121157986349683 2 36.45618615729925 5.7716091796069815 41.71018276762402 16.062021895469744 3 20.911776831111723 6.052173514726765 55.87810911089734 17.157940543264168 4 17.127021208373414 5.272319179148917 57.125188951491005 20.47547066098667 5 16.352892675553115 6.102560578993175 55.84031881269754 21.704227932756172 6 19.56735834363978 7.791672392469424 58.32417204892126 14.31679721496954 7 70.31743850487838 2.9167239246942422 22.83908203930191 3.9267555311254636 8 70.98163162475379 3.9279006916769728 21.329760432412627 3.760707251156612 9 66.18455407448124 5.197883743300811 22.78984013558701 5.827722046630938 10 34.205945673583436 21.409921671018274 29.376803627868625 15.007329027529659 11 26.618111859282674 21.665292474004854 34.83463881636205 16.88195685035042 12 22.24703403417159 20.76634144107004 39.08318446246164 17.90344006229673 13 21.49466355182997 19.73340662360863 41.28647336356558 17.48545646099583 14 19.942971004534833 21.13966378086208 41.4616829279465 17.45568228665659 15 19.42879391690715 19.467729375658468 41.927763272410786 19.17571343502359 16 21.377857175576015 19.928083917365214 39.95808712381476 18.73597178324401 17 21.199212129540562 19.70019696761486 39.39352297192066 19.707067930923916 18 22.151985708396317 18.603133159268932 39.64202281159819 19.60285832073657 19 21.846227841143328 20.855663964087764 37.594475745499516 19.70363244926939 20 23.320049470935825 22.244743713068573 37.09976638724749 17.33544042874811 21 23.27767853052998 22.779533690623424 36.84210526315789 17.1006825156887 22 22.29971141954102 20.229261142412167 37.85099170903761 19.620035729009206 23 20.93124456048738 22.115340570748018 37.34941138747652 19.604003481288075 24 20.370115890247813 22.41651779579497 37.16160505702899 20.05176125692822 25 20.64266410150703 23.858274930145207 35.562960927121985 19.936100041225778 26 20.175438596491226 22.629517658375704 36.73789565297055 20.457148092162523 27 19.85135816041409 21.92753424030049 37.43873391049426 20.78237368879117 28 18.48976226466951 22.97764646603454 37.3093307681737 21.223260501122258 29 20.265906280060463 22.45316293344327 36.46420228115982 20.81672850533645 30 21.53245385002977 21.574824790435617 37.32765333699785 19.56506802253676 31 21.719115019925795 22.947872291695294 35.54463835829783 19.788374330081076 32 21.595437680362785 22.645549906096836 34.704090513489994 21.054921900050388 33 22.095872841372362 23.509000961934863 33.07796253034675 21.317163666346023 34 21.364115248957905 24.5579680271174 33.34020429664239 20.737712427282304 35 21.23585726718886 25.572580275754657 32.52828546562228 20.6632769914342 36 21.788969813567864 26.477257111447024 31.052173514726768 20.68159956025835 37 21.44084100590903 25.72603178965691 30.914754248545645 21.918372955888415 38 21.545050616096376 26.086757363382347 31.2411250057258 21.127067014795475 39 22.227566304795932 25.817644633777654 29.818835600751225 22.135953460675186 40 22.92267875956209 25.974531629334436 30.582657688607945 20.520131922495533 41 20.843067198021163 26.547111905089093 30.276899821354952 22.332921075534788 42 20.994228390820393 26.211579863496866 31.372818469149376 21.42137327653337 43 21.579405432641657 24.785854976867757 31.170125051532228 22.46461453895836 44 21.953872932985206 25.438596491228072 30.59067381246851 22.01685676331822 45 21.977921304566898 25.05038706426641 30.224222435985524 22.747469195181164 46 21.613760249186935 25.518757729833723 31.219366955247125 21.648115065732217 47 20.34492235811461 26.393660391186845 32.36109202510192 20.900325225596628 48 20.3437771975631 26.290595941551004 32.166414731345334 21.199212129540562 49 21.62750217580505 25.040080619302824 32.043882552333834 21.28853465255829 50 20.972470340341715 25.16146763776281 31.370528148046358 22.495533873849112 51 20.339196555357063 24.820209793413035 30.634189913425864 24.20640373780404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 52.0 1 114.5 2 177.0 3 2025.0 4 3873.0 5 2595.5 6 1318.0 7 1279.5 8 1241.0 9 1250.0 10 1259.0 11 1274.0 12 1289.0 13 1235.0 14 1181.0 15 1112.5 16 1044.0 17 968.5 18 893.0 19 886.5 20 880.0 21 800.5 22 721.0 23 748.5 24 776.0 25 807.5 26 869.0 27 899.0 28 937.0 29 975.0 30 1100.5 31 1226.0 32 1367.0 33 1508.0 34 1643.5 35 1779.0 36 1953.0 37 2127.0 38 2319.0 39 2511.0 40 2670.0 41 2829.0 42 3208.5 43 3588.0 44 3774.5 45 3961.0 46 4792.5 47 5624.0 48 5822.5 49 6021.0 50 6056.0 51 6091.0 52 5777.5 53 5464.0 54 5072.5 55 4681.0 56 4612.0 57 4543.0 58 4286.0 59 4029.0 60 3760.5 61 3492.0 62 3094.5 63 2697.0 64 2475.0 65 2253.0 66 1952.0 67 1651.0 68 1421.5 69 1192.0 70 1075.5 71 959.0 72 802.5 73 646.0 74 543.5 75 369.5 76 298.0 77 217.5 78 137.0 79 114.0 80 91.0 81 65.5 82 40.0 83 26.0 84 12.0 85 8.5 86 5.0 87 5.5 88 6.0 89 3.5 90 1.0 91 1.5 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 87324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.44597132517978 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20688290486947 54.3115294764326 2 13.53659944263971 18.80124593468004 3 4.757350394934288 9.911364573313179 4 1.893046188348202 5.258577252530805 5 0.7634846561021058 2.6510466767440795 6 0.3297989875171083 1.374192661811186 7 0.15995250894579754 0.7775640144748294 8 0.08739673169203371 0.48554807383995235 9 0.04287386837722408 0.26796756905318125 >10 0.20447537226060716 2.4380468141633456 >50 0.00989396962551325 0.44088681233108884 >100 0.006595979750342167 0.6607576382208786 >500 0.0 0.0 >1k 0.0016489949375855417 2.621272502404837 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 2.621272502404837 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC 176 0.2015482570656406 RNA PCR Primer, Index 21 (95% over 23bp) GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG 148 0.1694837616233796 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC 133 0.15230635335073978 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC 120 0.1374192661811186 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0950483257752737 0.0 2 0.0 0.0 0.0 0.32179011497411936 0.0 3 0.0 0.0 0.0 0.5004351610095735 0.0 4 0.0 0.0 0.0 0.783289817232376 0.0 5 0.0 0.0 0.0 1.4784022719985341 0.0 6 0.0 0.0 0.0 2.086482524849984 0.0 7 0.0 0.0 0.0 2.5594338326233337 0.0 8 0.0 0.0 0.0 3.126288305620448 0.0 9 0.0011451605515093216 0.0 0.0 3.508771929824561 0.0 10 0.0011451605515093216 0.0 0.0 4.345884292977876 0.0 11 0.0011451605515093216 0.0 0.0 5.369657826027209 0.0 12 0.0011451605515093216 0.0 0.0 6.241125005725803 0.0 13 0.0011451605515093216 0.0 0.0 6.61673766662086 0.0 14 0.0011451605515093216 0.0 0.0 6.790802070450277 0.0 15 0.0011451605515093216 0.0 0.0 7.035866428473272 0.0 16 0.0011451605515093216 0.0 0.0 7.388575878338143 0.0 17 0.0011451605515093216 0.0 0.0 7.745865970409051 0.0 18 0.0011451605515093216 0.0 0.0 8.065365764280152 0.0 19 0.0011451605515093216 0.0 0.0 8.301268837891072 0.0 20 0.0011451605515093216 0.0 0.0 8.510833218817279 0.0 21 0.0011451605515093216 0.0 0.0 8.72841372360405 0.0 22 0.0011451605515093216 0.0 0.0 8.92881682011818 0.0 23 0.0011451605515093216 0.0 0.0 9.124639274426274 0.0 24 0.0011451605515093216 0.0 0.0 9.263203701158902 0.0 25 0.0011451605515093216 0.0 0.0 9.41894553616417 0.0 26 0.0011451605515093216 0.0 0.0 9.546058357381705 0.0 27 0.0011451605515093216 0.0 0.0 9.653703449223581 0.0 28 0.0011451605515093216 0.0 0.0 9.785396912647153 0.0 29 0.0011451605515093216 0.0 0.0 9.918235536622234 0.0 30 0.0011451605515093216 0.0 0.0 10.067106408318446 0.0 31 0.0011451605515093216 0.0 0.0 10.204525674499564 0.0 32 0.0011451605515093216 0.0 0.0 10.395767486601622 0.0 33 0.0011451605515093216 0.0 0.0 10.561815766570474 0.0 34 0.0011451605515093216 0.0 0.0 10.723283404333287 0.0 35 0.0011451605515093216 0.0 0.0 10.900783289817232 0.0 36 0.0011451605515093216 0.0 0.0 11.018734826622692 0.0 37 0.0011451605515093216 0.0 0.0 11.159589574458339 0.0 38 0.0011451605515093216 0.0 0.0 11.357702349869452 0.0 39 0.0011451605515093216 0.0 0.0 11.704685996976776 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTTG 25 3.8611615E-5 45.000004 1 TATTGGG 25 3.8611615E-5 45.000004 3 ATCCTAG 20 6.997487E-4 45.0 14 TGGGTAC 20 6.997487E-4 45.0 6 GCCCTAG 20 6.997487E-4 45.0 20 GGAACTA 20 6.997487E-4 45.0 8 TTCGGGT 20 6.997487E-4 45.0 4 GACTAAT 20 6.997487E-4 45.0 9 CGTTTTT 1355 0.0 43.67159 1 TGGGACA 55 6.002665E-11 40.90909 6 AGCGGGG 35 6.1822175E-6 38.571426 4 GTTTTTT 1590 0.0 38.490566 2 CGGGTGA 30 1.1313037E-4 37.500004 6 GGAATGA 60 1.5097612E-10 37.500004 8 CCGGGTG 30 1.1313037E-4 37.500004 5 GGGACTA 30 1.1313037E-4 37.500004 7 GGGACCG 60 1.5097612E-10 37.500004 7 TGTCTTG 30 1.1313037E-4 37.500004 1 GCGATTC 30 1.1313037E-4 37.500004 9 GGATTAA 30 1.1313037E-4 37.500004 8 >>END_MODULE