FastQCFastQC Report
Sat 14 Jan 2017
SRR2933619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88293
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22282.523416352372215No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC1610.1823474114595721RNA PCR Primer, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC1590.18008222622404946No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG1560.17668444837076552No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC1170.13251333627807416No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC960.10872889130508648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG302.1427204E-645.00000414
ATACTAT302.1427204E-645.00000445
GCCCTAC302.1427204E-645.00000426
ACTAATC302.1427204E-645.00000410
CTAATCC302.1427204E-645.00000411
TAATCCT302.1427204E-645.00000412
CACATCC206.997903E-445.045
TGCGGGC253.861507E-545.04
TGTTGCG206.997903E-445.01
AACATGG551.8189894E-1245.010
GGAAATA206.997903E-445.08
CTTTCGG253.861507E-545.02
AGGGCGA206.997903E-445.06
CGTTTTT13600.043.6764681
TCAAGCG556.002665E-1140.90909217
AGTTGGG501.0568328E-940.53
GGCGATC501.0568328E-940.58
TTTGGGC2450.040.4081654
GTCTTTG451.8939318E-840.01
GCGATGT403.4100958E-739.3759