##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933619.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88293
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.255558198271665	33.0	31.0	34.0	30.0	34.0
2	32.424824164996096	34.0	31.0	34.0	31.0	34.0
3	32.53468564891894	34.0	31.0	34.0	31.0	34.0
4	36.01351182992989	37.0	35.0	37.0	35.0	37.0
5	36.00505136307522	37.0	35.0	37.0	35.0	37.0
6	35.96440261402376	37.0	35.0	37.0	35.0	37.0
7	35.93411708742482	37.0	35.0	37.0	35.0	37.0
8	35.7973791806825	37.0	35.0	37.0	35.0	37.0
9	37.631465688106644	39.0	38.0	39.0	35.0	39.0
10	37.20258684153897	39.0	37.0	39.0	34.0	39.0
11	37.10961231354694	39.0	37.0	39.0	34.0	39.0
12	37.01762314113237	39.0	37.0	39.0	33.0	39.0
13	36.97470920684539	39.0	37.0	39.0	33.0	39.0
14	38.146897262523645	40.0	38.0	41.0	33.0	41.0
15	38.217242590012795	40.0	38.0	41.0	33.0	41.0
16	38.307295029051	40.0	38.0	41.0	33.0	41.0
17	38.291653924999714	40.0	38.0	41.0	33.0	41.0
18	38.08167125366677	39.0	37.0	41.0	34.0	41.0
19	37.846137292877124	39.0	37.0	41.0	34.0	41.0
20	37.44431608394776	39.0	35.0	41.0	33.0	41.0
21	37.36981414155142	39.0	35.0	41.0	33.0	41.0
22	37.342144903899516	39.0	35.0	41.0	33.0	41.0
23	37.185699885608145	39.0	35.0	41.0	33.0	41.0
24	37.0846386463253	39.0	35.0	41.0	33.0	41.0
25	37.001211874101	39.0	35.0	41.0	33.0	41.0
26	36.94443500617263	39.0	35.0	41.0	33.0	41.0
27	36.836782077854416	38.0	35.0	41.0	33.0	41.0
28	36.74060231275413	38.0	35.0	40.0	32.0	41.0
29	36.56119964210073	38.0	35.0	40.0	31.0	41.0
30	36.30834834018552	38.0	35.0	40.0	31.0	41.0
31	36.02375046719445	38.0	35.0	40.0	30.0	41.0
32	35.686328474511	38.0	35.0	40.0	28.0	41.0
33	35.1878971152866	38.0	35.0	40.0	24.0	41.0
34	34.743739594305325	38.0	34.0	41.0	21.0	41.0
35	34.470071240075654	38.0	34.0	41.0	18.0	41.0
36	34.27536724315631	38.0	34.0	40.0	18.0	41.0
37	34.15607126272751	38.0	34.0	40.0	18.0	41.0
38	34.02658194873886	38.0	34.0	40.0	16.0	41.0
39	33.947402398831166	38.0	34.0	40.0	15.0	41.0
40	33.770831209722175	38.0	33.0	40.0	15.0	41.0
41	33.61873534708301	38.0	33.0	40.0	15.0	41.0
42	33.44365918022946	37.0	33.0	40.0	14.0	41.0
43	33.35640424495713	37.0	33.0	40.0	12.0	41.0
44	33.21360696770979	37.0	33.0	40.0	12.0	41.0
45	33.15276409228365	37.0	33.0	40.0	12.0	41.0
46	33.00029447408062	36.0	32.0	40.0	12.0	41.0
47	32.94758361365	36.0	32.0	40.0	12.0	41.0
48	32.833372974074955	36.0	32.0	40.0	11.0	41.0
49	32.787457669350914	36.0	32.0	40.0	12.0	41.0
50	32.61796518410293	36.0	32.0	40.0	10.0	41.0
51	31.76007157985344	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	1.0
12	7.0
13	4.0
14	3.0
15	6.0
16	7.0
17	30.0
18	56.0
19	89.0
20	166.0
21	237.0
22	299.0
23	554.0
24	860.0
25	1486.0
26	2267.0
27	2639.0
28	2498.0
29	2258.0
30	2179.0
31	2266.0
32	2565.0
33	3432.0
34	5515.0
35	6123.0
36	7365.0
37	9954.0
38	16281.0
39	19136.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.644320614318236	4.4895971368058625	37.620196391559915	30.24588585731598
2	36.478543032856514	5.8271890183819774	41.58993351681334	16.10433443194817
3	20.79666564733331	6.127326062088727	56.055406430860884	17.020601859717075
4	17.04891667516111	5.20199789337773	56.92183978344829	20.827245648012866
5	16.262897398434756	6.0639008754940935	55.68051827438188	21.992683451689263
6	19.71277451213573	7.855662396792498	58.376088704653824	14.055474386417949
7	70.25358748711676	2.9583319175925613	22.79908939553532	3.98899119975536
8	70.96032528059982	3.8349586037398207	21.43997825422174	3.764737861438619
9	65.8987688718245	5.206528263848776	23.04939236406057	5.845310500266159
10	34.012888903990124	21.642712332801015	29.619562139693972	14.72483662351489
11	26.084740579660902	21.69820937107132	35.079791149921284	17.137258899346495
12	21.83525307782044	20.86575379701675	39.196765315483674	18.102227809679135
13	21.684618259658183	19.642553769834528	41.24336017577838	17.429467794728914
14	19.81584044035201	20.647163421788818	41.73717055712231	17.799825580736865
15	19.320897466390313	19.741089327579765	42.16076019616504	18.77725300986488
16	21.458099736105922	20.15901600353369	39.752868290804486	18.63001596955591
17	21.19307306354977	19.934762665216947	39.350797911499214	19.521366359734067
18	22.11953382487853	18.677584859501888	39.47878087730624	19.724100438313343
19	21.80014270666984	20.866886389634512	37.97469788091921	19.358273022776437
20	23.50356200378286	22.199947900739584	37.01312674843986	17.283363347037703
21	22.916879027782496	22.504615314917377	37.3336504592663	17.24485519803382
22	22.555581982716635	20.24396044986579	37.83085861846352	19.36959894895405
23	20.69133453388151	22.41287531287871	37.4480423136602	19.44774783957958
24	20.34929156331759	22.269036050423026	37.27928601361376	20.102386372645622
25	20.550893049279104	23.90903016094141	35.677800052099265	19.862276737680222
26	20.367413045201772	22.40268197931886	36.61898451745891	20.610920458020455
27	19.99592266657606	22.07762789802136	37.23624749413884	20.690201941263748
28	18.649270044057854	22.84212791501025	37.23284971628555	21.275752324646348
29	20.435368602267452	22.34378716319527	36.47401266238547	20.746831572151812
30	21.610999739503697	21.536248626731453	37.42992083177602	19.422830801988834
31	21.563430849557722	22.68583013375919	35.942826724655404	19.80791229202768
32	21.726524186515352	22.60994642836918	34.61203039878586	21.051498986329605
33	22.164837529588983	23.505827189018383	33.2551844427078	21.074150838684833
34	21.32218862197456	24.470796099351023	33.57231037568097	20.63470490299344
35	21.153432321928126	25.983939836680143	32.14637626991947	20.71625157147226
36	21.64724270327206	26.499269477761544	31.329776992513565	20.523710826452835
37	21.220255286376045	25.904658353436854	31.359224400575357	21.515861959611748
38	22.14558345508704	25.846896129931025	31.035302911895617	20.972217503086316
39	22.469504943766776	25.747227979568027	30.008041407586106	21.77522566907909
40	23.057320512384898	25.623775384232044	30.738563646042156	20.5803404573409
41	20.95183083596661	26.567225034827224	30.261742153964637	22.219201975241525
42	21.186277507843204	26.08247539442538	31.33430736298461	21.39693973474681
43	21.31992343673904	24.88192721959838	31.58574292412762	22.212406419534958
44	21.752573816723864	25.391593897590976	30.707983645362603	22.14784864032256
45	22.06969974969703	25.18546204115842	30.005776222350583	22.73906198679397
46	21.400337512600093	25.69626131176877	31.52005255229746	21.383348623333674
47	20.296059710282808	26.311259103213164	32.29474590284621	21.09793528365782
48	20.488600455302233	26.20706058237912	32.086348861178124	21.217990101140522
49	21.729921964368636	25.013307963258697	31.987813303432887	21.26895676893978
50	20.694732311734793	25.626040569467566	31.313920695864905	22.365306422932736
51	20.38440193446819	25.19225759686498	30.798591054783504	23.62474941388332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	109.0
2	173.0
3	2030.5
4	3888.0
5	2635.0
6	1382.0
7	1356.0
8	1330.0
9	1302.0
10	1274.0
11	1280.0
12	1286.0
13	1211.5
14	1137.0
15	1109.5
16	1082.0
17	1021.0
18	960.0
19	895.0
20	830.0
21	792.5
22	755.0
23	724.0
24	693.0
25	744.5
26	831.0
27	866.0
28	951.5
29	1037.0
30	1167.5
31	1298.0
32	1438.0
33	1578.0
34	1745.0
35	1912.0
36	2044.0
37	2176.0
38	2394.5
39	2613.0
40	2800.0
41	2987.0
42	3300.0
43	3613.0
44	3826.5
45	4040.0
46	4855.5
47	5671.0
48	5883.5
49	6096.0
50	6047.0
51	5998.0
52	5649.0
53	5300.0
54	5099.0
55	4898.0
56	4674.0
57	4450.0
58	4267.0
59	4084.0
60	3811.0
61	3538.0
62	3177.0
63	2816.0
64	2492.5
65	2169.0
66	1918.0
67	1667.0
68	1457.5
69	1248.0
70	1095.5
71	943.0
72	780.0
73	617.0
74	529.0
75	367.5
76	294.0
77	232.5
78	171.0
79	129.0
80	87.0
81	58.0
82	29.0
83	22.0
84	15.0
85	10.5
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	88293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.73599266079984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45936464626779	54.71441677143149
2	13.277138959267198	18.517889300397538
3	4.828493471058273	10.10159355781319
4	1.8742285454427337	5.228047523586241
5	0.7617098681218736	2.6559296886502897
6	0.3102059377639187	1.2979511399544699
7	0.15429091145325796	0.7531740908112761
8	0.07633339829792762	0.4258548242782554
9	0.037354641720262455	0.23444667187659268
>10	0.20301435717533944	2.401096349653993
>50	0.009744689144416292	0.474556306841992
>100	0.006496459429610862	0.6716274223324613
>500	0.0	0.0
>1k	0.0016241148574027155	2.523416352372215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2228	2.523416352372215	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	161	0.1823474114595721	RNA PCR Primer, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	159	0.18008222622404946	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	156	0.17668444837076552	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	117	0.13251333627807416	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	96	0.10872889130508648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10193333559851857	0.0
2	0.0	0.0	0.0	0.29560667323570383	0.0
3	0.0	0.0	0.0	0.4417111209269138	0.0
4	0.0	0.0	0.0	0.6920140894521649	0.0
5	0.0	0.0	0.0	1.3613763265491035	0.0
6	0.0	0.0	0.0	1.948059302549466	0.0
7	0.0	0.0	0.0	2.429411165098026	0.0
8	0.0	0.0	0.0	3.0387459934536145	0.0
9	0.0	0.0	0.0	3.437418594905598	0.0
10	0.0	0.0	0.0	4.21211194545434	0.0
11	0.0	0.0	0.0	5.205395671231015	0.0
12	0.0	0.0	0.0	6.053707541934242	0.0
13	0.0	0.0	0.0	6.435391254119805	0.0
14	0.0	0.0	0.0	6.6505838514944555	0.0
15	0.0	0.0	0.0	6.8646438562513445	0.0
16	0.0	0.0	0.0	7.153454973780481	0.0
17	0.0	0.0	0.0	7.521547574552909	0.0
18	0.0	0.0	0.0	7.855662396792498	0.0
19	0.0	0.0	0.0	8.105965365317749	0.0
20	0.0	0.0	0.0	8.316627592221353	0.0
21	0.0	0.0	0.0	8.517096485565107	0.0
22	0.0	0.0	0.0	8.72662611985095	0.0
23	0.0	0.0	0.0	8.905575753457239	0.0
24	0.0	0.0	0.0	9.05394538638397	0.0
25	0.0	0.0	0.0	9.175132796484432	0.0
26	0.0	0.0	0.0	9.30085057705594	0.0
27	0.0	0.0	0.0	9.43109872809849	0.0
28	0.0	0.0	0.0	9.56021428652328	0.0
29	0.0	0.0	0.0	9.694992808036877	0.0
30	0.0	0.0	0.0	9.852423181905701	0.0
31	0.0	0.0	0.0	9.99059948127258	0.0
32	0.0	0.0	0.0	10.19673133770514	0.0
33	0.0	0.0	0.0	10.336040229689782	0.0
34	0.0	0.0	0.0	10.48101208476323	0.0
35	0.0	0.0	0.0	10.649768384809668	0.0
36	0.0	0.0	0.0	10.800403202971923	0.0
37	0.0	0.0	0.0	10.937446909721043	0.0
38	0.0	0.0	0.0	11.094877283589865	0.0
39	0.0	0.0	0.0	11.40067729038542	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	30	2.1427204E-6	45.000004	14
ATACTAT	30	2.1427204E-6	45.000004	45
GCCCTAC	30	2.1427204E-6	45.000004	26
ACTAATC	30	2.1427204E-6	45.000004	10
CTAATCC	30	2.1427204E-6	45.000004	11
TAATCCT	30	2.1427204E-6	45.000004	12
CACATCC	20	6.997903E-4	45.0	45
TGCGGGC	25	3.861507E-5	45.0	4
TGTTGCG	20	6.997903E-4	45.0	1
AACATGG	55	1.8189894E-12	45.0	10
GGAAATA	20	6.997903E-4	45.0	8
CTTTCGG	25	3.861507E-5	45.0	2
AGGGCGA	20	6.997903E-4	45.0	6
CGTTTTT	1360	0.0	43.676468	1
TCAAGCG	55	6.002665E-11	40.909092	17
AGTTGGG	50	1.0568328E-9	40.5	3
GGCGATC	50	1.0568328E-9	40.5	8
TTTGGGC	245	0.0	40.408165	4
GTCTTTG	45	1.8939318E-8	40.0	1
GCGATGT	40	3.4100958E-7	39.375	9
>>END_MODULE