##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933618.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83583
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03607192850221	33.0	31.0	34.0	30.0	34.0
2	32.22577557637319	34.0	31.0	34.0	30.0	34.0
3	32.31031429836211	34.0	31.0	34.0	30.0	34.0
4	35.88416304750966	37.0	35.0	37.0	35.0	37.0
5	35.88370840960482	37.0	35.0	37.0	35.0	37.0
6	35.83378198916048	37.0	35.0	37.0	35.0	37.0
7	35.85720780541498	37.0	35.0	37.0	35.0	37.0
8	35.675077467906156	37.0	35.0	37.0	35.0	37.0
9	37.49368890803154	39.0	37.0	39.0	35.0	39.0
10	37.019776748860416	39.0	37.0	39.0	33.0	39.0
11	36.99179258940215	39.0	37.0	39.0	33.0	39.0
12	36.92942344735173	39.0	37.0	39.0	33.0	39.0
13	36.82168622806073	39.0	37.0	39.0	33.0	39.0
14	37.6707584078102	40.0	37.0	41.0	32.0	41.0
15	37.77205891150114	40.0	37.0	41.0	32.0	41.0
16	37.95630690451408	40.0	37.0	41.0	33.0	41.0
17	37.950145364487994	40.0	37.0	41.0	33.0	41.0
18	37.700477369800076	39.0	37.0	40.0	33.0	41.0
19	37.404855054257446	38.0	36.0	40.0	33.0	41.0
20	36.954703707691756	38.0	35.0	40.0	33.0	41.0
21	36.833147888924785	38.0	35.0	40.0	32.0	41.0
22	36.862507926252945	38.0	35.0	40.0	33.0	41.0
23	36.84161851094122	38.0	35.0	40.0	33.0	41.0
24	36.71691611930656	38.0	35.0	40.0	32.0	41.0
25	36.63803644281732	38.0	35.0	40.0	32.0	41.0
26	36.56037711017791	38.0	35.0	40.0	32.0	41.0
27	36.49036287283299	38.0	35.0	40.0	32.0	41.0
28	36.35331347283539	38.0	35.0	40.0	31.0	41.0
29	36.20761398849048	38.0	35.0	40.0	31.0	41.0
30	36.04761733845399	38.0	35.0	40.0	31.0	41.0
31	35.671105368316525	38.0	35.0	40.0	30.0	41.0
32	35.29291841642439	38.0	35.0	40.0	27.0	41.0
33	34.83551679169209	38.0	34.0	40.0	23.0	41.0
34	34.446526207482385	38.0	34.0	40.0	21.0	41.0
35	34.10219781534523	38.0	33.0	40.0	18.0	41.0
36	33.89184403527033	38.0	33.0	40.0	18.0	41.0
37	33.72198892119211	37.0	33.0	40.0	18.0	41.0
38	33.52395822116938	37.0	33.0	40.0	16.0	41.0
39	33.3134489070744	37.0	33.0	40.0	15.0	41.0
40	33.18570761997057	37.0	33.0	40.0	15.0	41.0
41	33.11892370458108	37.0	32.0	40.0	15.0	41.0
42	32.97337975425625	36.0	32.0	40.0	15.0	41.0
43	32.83997942165273	36.0	32.0	40.0	13.0	41.0
44	32.6755201416556	36.0	31.0	40.0	12.0	41.0
45	32.66489597166888	36.0	31.0	40.0	12.0	41.0
46	32.436703635906824	35.0	31.0	40.0	12.0	41.0
47	32.18152016558391	35.0	31.0	39.0	12.0	41.0
48	32.07082779991146	35.0	31.0	39.0	11.0	41.0
49	32.073735089671345	35.0	31.0	39.0	10.0	41.0
50	31.90597370278645	35.0	31.0	39.0	10.0	40.0
51	30.838483902228923	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	5.0
14	3.0
15	7.0
16	10.0
17	24.0
18	43.0
19	75.0
20	129.0
21	231.0
22	343.0
23	481.0
24	839.0
25	1409.0
26	2281.0
27	2451.0
28	2377.0
29	2243.0
30	2352.0
31	2557.0
32	3023.0
33	4078.0
34	5914.0
35	6921.0
36	8517.0
37	10771.0
38	15279.0
39	11210.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.598889726379767	5.94259598243662	40.8504121651532	31.608102126030413
2	36.36146106265628	5.136211909120276	40.43764880418267	18.064678224040772
3	18.577940490291088	5.221157412392472	56.821363195865196	19.37953890145125
4	15.884809111900747	5.605206800425925	56.51627723340871	21.993706854264623
5	13.715707739612121	6.6688202146369475	57.6109974516349	22.004474594116026
6	18.849526817654308	7.141404352559731	60.624768194489306	13.38430063529665
7	64.61122477058731	3.442087505832526	27.15145424308771	4.795233480492445
8	63.54043286314203	3.745977052749961	26.457533230441598	6.256056853666416
9	58.187669741454606	5.90431068518718	29.202110477010873	6.705909096347343
10	31.237213308926453	19.36637833052176	34.818085017288205	14.578323343263582
11	22.68164578921551	17.7464316906548	39.60015792685115	19.971764593278536
12	20.152423339674336	15.437349700297908	45.03427730519364	19.37594965483412
13	22.345453022743857	16.398071378151062	46.318031178588946	14.938444420516134
14	18.335068135864947	18.308746994005958	46.99759520476652	16.358589665362576
15	14.50893124199897	17.255901319646338	48.68693394589809	19.5482334924566
16	16.701960925068494	17.394685522175564	44.589210724668895	21.31414282808705
17	16.758192455403613	17.68900374478064	42.57923261907325	22.973571180742493
18	17.99767895385425	16.64333656365529	45.39080913582906	19.9681753466614
19	17.6375578766017	19.062488783604323	45.27714965961978	18.0228036801742
20	16.59428352655444	23.002285153679576	43.09967337855784	17.30375794120814
21	15.757989064761974	22.801287343120013	44.528193532177596	16.912530059940416
22	14.016008039912423	20.933682686670736	43.8318796884534	21.21842958496345
23	13.987294066975341	21.59051481760645	41.935561059067034	22.486630056351174
24	14.912123278657145	22.38852398214948	40.95689314812821	21.74245959106517
25	15.919505162533051	22.980749673976767	38.15369154014572	22.946053623344458
26	14.609430147278754	22.857518873455128	39.58101527822643	22.952035701039687
27	15.512723879257745	21.467284017084815	42.605553760932246	20.414438342725195
28	14.944426498211358	20.597489920199084	40.63984303028128	23.818240551308282
29	16.77494227295024	21.181340703253053	39.12518095785028	22.918536065946423
30	21.603675388535944	21.157412392472153	37.5471088618499	19.691803357142
31	21.01623535886484	24.824426019645145	34.412500149551946	19.746838471938073
32	20.08901331610495	25.134297644257803	32.194345740162476	22.582343299474775
33	20.701578072095998	23.67706351770097	31.282677099410165	24.338681310792865
34	21.128698419535073	21.943457401624734	33.50202792433868	23.425816254501513
35	21.212447507268227	23.923525118744244	30.388954691743535	24.475072682243997
36	23.24156826148858	25.591328380173	28.520153619755213	22.646949738583206
37	22.349042269360993	26.182357656461242	29.940298864601655	21.52830120957611
38	21.59290764868454	29.041790794778844	28.387351494921216	20.9779500616154
39	19.992103657442303	29.234413696565092	29.545481736716795	21.22800090927581
40	20.77216658889966	24.648552935405522	34.17560987282103	20.40367060287379
41	18.88781211490375	23.106373305576493	31.55426342677339	26.451551152746372
42	19.90715815417011	25.14506538410921	30.901020542454805	24.04675591926588
43	20.592704258042904	24.062309321273464	33.46972470478447	21.875261715899168
44	20.86070133878899	23.266692987808526	32.32714786499647	23.545457808406013
45	22.158812198652836	21.442159290764867	31.16423196104471	25.234796549537585
46	22.381345488915212	21.38233851381262	34.86713805438905	21.369177942883123
47	17.657896940765465	25.101994424703587	36.811313305337215	20.428795329193736
48	17.083617482023858	25.444169268870464	36.12217795484728	21.3500352942584
49	21.135876912769344	21.679049567495785	36.557673211059665	20.62740030867521
50	19.66548221528301	19.88203342785016	34.64939042628286	25.80309393058397
51	17.754806599428115	19.631982580189753	32.7159829151861	29.897227905196033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	543.5
2	737.0
3	3187.0
4	5637.0
5	3468.5
6	1300.0
7	1262.0
8	1224.0
9	1185.0
10	1146.0
11	1136.5
12	1127.0
13	1057.0
14	987.0
15	943.5
16	900.0
17	799.0
18	698.0
19	676.5
20	655.0
21	632.0
22	609.0
23	581.5
24	554.0
25	562.5
26	560.5
27	550.0
28	592.0
29	634.0
30	680.0
31	726.0
32	788.5
33	851.0
34	988.5
35	1126.0
36	1234.5
37	1343.0
38	1403.0
39	1463.0
40	1844.0
41	2225.0
42	2636.5
43	3048.0
44	3611.5
45	4175.0
46	5291.0
47	6407.0
48	7966.5
49	9526.0
50	9701.5
51	9877.0
52	8528.5
53	7180.0
54	5805.0
55	4430.0
56	3728.0
57	3026.0
58	2761.5
59	2497.0
60	2195.5
61	1894.0
62	1765.5
63	1637.0
64	1527.5
65	1418.0
66	1220.5
67	1023.0
68	850.0
69	677.0
70	591.5
71	506.0
72	427.0
73	348.0
74	283.0
75	176.5
76	135.0
77	104.5
78	74.0
79	58.0
80	42.0
81	30.0
82	18.0
83	13.0
84	8.0
85	5.0
86	2.0
87	2.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	83583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.25947860210809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68348538447216	51.20419224004882
2	11.729659281325636	15.074835791967267
3	4.330664680692609	8.348587631456157
4	1.850679575498045	4.756948183243004
5	0.837832805808974	2.6919349628512976
6	0.46732452057345003	1.8018018018018018
7	0.23645503630608825	1.0636134142110236
8	0.16384285980264382	0.842276539487695
9	0.11729659281325638	0.678367610638527
>10	0.5436603984360454	6.011988083701231
>50	0.022342208154905976	1.016953208188268
>100	0.014894805436603984	2.139190983812498
>500	0.0	0.0
>1k	0.001861850679575498	4.369309548592417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3652	4.369309548592417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC	439	0.5252264216407643	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	302	0.3613174927915964	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	220	0.26321141858990466	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.22731895241855402	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	168	0.20099781055956356	No Hit
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGCT	161	0.1926229017862484	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	156	0.18664082409102328	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	152	0.18185516193484322	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98	0.11724872282641208	No Hit
TCCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	88	0.10528456743596187	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85	0.10169532081882679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002392831078090042	0.0	0.0	0.21056913487192372	0.0
2	0.002392831078090042	0.0	0.0	1.0923273871481043	0.0
3	0.002392831078090042	0.0	0.0	1.6295179641793187	0.0
4	0.002392831078090042	0.0	0.0	2.4143665577928526	0.0
5	0.002392831078090042	0.0	0.0	4.082169819221612	0.0
6	0.002392831078090042	0.0	0.0	5.583671320723114	0.0
7	0.002392831078090042	0.0	0.0	6.8363183901032505	0.0
8	0.002392831078090042	0.0	0.0	8.846296495698887	0.0
9	0.002392831078090042	0.0	0.0	9.9362310517689	0.0
10	0.002392831078090042	0.0	0.0	11.998851441082516	0.0
11	0.002392831078090042	0.0	0.0	16.82519172559013	0.0
12	0.002392831078090042	0.0	0.0	21.05212782503619	0.0
13	0.002392831078090042	0.0	0.0	22.497397796202577	0.0
14	0.002392831078090042	0.0	0.0	23.050141775241375	0.0
15	0.002392831078090042	0.0	0.0	23.793115824988334	0.0
16	0.002392831078090042	0.0	0.0	25.464508333034228	0.0
17	0.002392831078090042	0.0	0.0	27.51157532034026	0.0
18	0.002392831078090042	0.0	0.0	29.349269588313412	0.0
19	0.002392831078090042	0.0	0.0	30.524149647655623	0.0
20	0.002392831078090042	0.0	0.0	31.621262696959906	0.0
21	0.002392831078090042	0.0	0.0	32.84160654678583	0.0
22	0.002392831078090042	0.0	0.0	33.94589808932439	0.0
23	0.002392831078090042	0.0	0.0	34.66015816613426	0.0
24	0.002392831078090042	0.0	0.0	35.09326059126856	0.0
25	0.002392831078090042	0.0	0.0	35.357668425397506	0.0
26	0.002392831078090042	0.0	0.0	35.61609418183124	0.0
27	0.002392831078090042	0.0	0.0	35.851788043023106	0.0
28	0.002392831078090042	0.0	0.0	36.05876793127789	0.0
29	0.002392831078090042	0.0	0.0	36.30642594786021	0.0
30	0.002392831078090042	0.0	0.0	36.513405836115	0.0
31	0.002392831078090042	0.0	0.0	36.65936853187849	0.0
32	0.002392831078090042	0.0	0.0	36.82806312288384	0.0
33	0.002392831078090042	0.0	0.0	36.98957922065492	0.0
34	0.002392831078090042	0.0	0.0	37.157077396121224	0.0
35	0.002392831078090042	0.0	0.0	37.36764653099314	0.0
36	0.0035892466171350635	0.0	0.0	37.54471603077181	0.0
37	0.0035892466171350635	0.0	0.0	37.689482310996254	0.0
38	0.0035892466171350635	0.0	0.0	37.90483710802436	0.0
39	0.0035892466171350635	0.0	0.0	38.44202768505557	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTCA	20	6.9957494E-4	45.000004	7
GGTTCAT	20	6.9957494E-4	45.000004	8
TGGGTTC	30	2.141338E-6	45.000004	6
TGGGCCA	20	6.9957494E-4	45.000004	6
TGCGGGT	20	6.9957494E-4	45.000004	4
GGAGCAA	20	6.9957494E-4	45.000004	8
GCGGGCT	25	3.8597267E-5	45.0	5
GCGATTT	25	3.8597267E-5	45.0	9
TGGGACC	45	3.765308E-10	45.0	6
CTTTTTG	45	3.765308E-10	45.0	1
CGTTTTT	1350	0.0	44.833332	1
TTGGGAC	115	0.0	43.04348	5
TGGGACT	100	0.0	42.75	6
CTTGGGA	165	0.0	40.90909	4
TTGGGAG	160	0.0	40.781254	5
TCTGGGT	50	1.0568328E-9	40.5	4
GCGGGGT	45	1.891749E-8	40.0	5
CGGGCTG	45	1.891749E-8	40.0	6
GTTTTTT	1685	0.0	39.52522	2
CGCTTGG	40	3.4071854E-7	39.375004	2
>>END_MODULE