##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24786
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32264181392722	33.0	31.0	34.0	31.0	34.0
2	32.47038650851287	34.0	31.0	34.0	31.0	34.0
3	32.595295731461306	34.0	31.0	34.0	31.0	34.0
4	36.072419914467844	37.0	35.0	37.0	35.0	37.0
5	36.054587267005566	37.0	35.0	37.0	35.0	37.0
6	36.00576938594368	37.0	35.0	37.0	35.0	37.0
7	36.020051642055996	37.0	35.0	37.0	35.0	37.0
8	35.85310255789559	37.0	35.0	37.0	35.0	37.0
9	37.69821673525377	39.0	38.0	39.0	35.0	39.0
10	37.232631324134594	39.0	37.0	39.0	34.0	39.0
11	37.206931332203666	39.0	37.0	39.0	34.0	39.0
12	37.19708706527879	39.0	37.0	39.0	34.0	39.0
13	37.19934640522876	39.0	37.0	39.0	34.0	39.0
14	38.35879125312677	40.0	38.0	41.0	34.0	41.0
15	38.444323408375695	40.0	38.0	41.0	34.0	41.0
16	38.477850399419026	40.0	38.0	41.0	34.0	41.0
17	38.42507867344469	40.0	38.0	41.0	34.0	41.0
18	38.21419349632857	39.0	38.0	41.0	34.0	41.0
19	38.004922133462436	39.0	37.0	41.0	34.0	41.0
20	37.73715000403453	39.0	35.0	41.0	34.0	41.0
21	37.636205922698295	39.0	35.0	41.0	33.0	41.0
22	37.6080045186799	39.0	35.0	41.0	33.0	41.0
23	37.53925603163076	39.0	35.0	41.0	33.0	41.0
24	37.45800048414427	39.0	35.0	41.0	33.0	41.0
25	37.361413701282984	39.0	35.0	41.0	33.0	41.0
26	37.214032114903574	39.0	35.0	41.0	33.0	41.0
27	37.160372791091746	39.0	35.0	41.0	33.0	41.0
28	37.03808601629952	39.0	35.0	41.0	33.0	41.0
29	36.89707899620754	39.0	35.0	41.0	32.0	41.0
30	36.81848624223352	39.0	35.0	41.0	32.0	41.0
31	36.60263858629872	39.0	35.0	41.0	31.0	41.0
32	36.32502218994594	39.0	35.0	41.0	30.0	41.0
33	36.07782619220528	39.0	35.0	41.0	30.0	41.0
34	35.77301702574034	39.0	35.0	41.0	27.0	41.0
35	35.51424191075607	39.0	35.0	41.0	25.0	41.0
36	35.39481965625757	39.0	35.0	41.0	24.0	41.0
37	35.29399661099008	38.0	35.0	41.0	24.0	41.0
38	35.13677075768579	38.0	35.0	40.0	23.0	41.0
39	35.07209715161785	38.0	35.0	40.0	23.0	41.0
40	34.91749374646978	38.0	35.0	40.0	23.0	41.0
41	34.784676833696444	38.0	35.0	40.0	22.0	41.0
42	34.69047042685387	38.0	34.0	40.0	22.0	41.0
43	34.498749293956266	38.0	34.0	40.0	21.0	41.0
44	34.34636488340192	38.0	34.0	40.0	21.0	41.0
45	34.33139675623336	37.0	34.0	40.0	21.0	41.0
46	34.134188654885826	37.0	33.0	40.0	20.0	41.0
47	33.97292826595659	37.0	33.0	40.0	20.0	41.0
48	33.90099249576374	37.0	33.0	40.0	20.0	41.0
49	33.8558863874768	37.0	33.0	40.0	20.0	41.0
50	33.799604615508756	36.0	33.0	40.0	20.0	41.0
51	32.800209795852496	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	5.0
16	3.0
17	5.0
18	12.0
19	20.0
20	26.0
21	35.0
22	61.0
23	117.0
24	191.0
25	289.0
26	427.0
27	540.0
28	477.0
29	482.0
30	485.0
31	543.0
32	680.0
33	925.0
34	1442.0
35	1894.0
36	2421.0
37	3152.0
38	5163.0
39	5387.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.11498426531106	5.357863309933027	40.998951020737515	28.5282014040184
2	33.700476075203746	5.474864843056564	44.420237230694745	16.404421851044944
3	20.789962075365125	5.345759703058178	55.34172516743323	18.52255305414347
4	17.453401113531832	5.809731299927378	54.93423706931332	21.802630517227467
5	15.185992092310174	6.7175018155410315	53.7198418462035	24.376664245945292
6	19.172113289760347	7.451787299281852	58.43621399176955	14.93988541918825
7	69.49487613975631	3.586702170580166	21.907528443476156	5.010893246187364
8	68.6798999435165	4.502541757443718	21.370935205357863	5.446623093681917
9	62.90244492858872	6.588396675542644	23.412410231582346	7.09674816428629
10	30.86823206648915	21.927701121600904	30.3598805777455	16.84418623416445
11	23.125958202210924	21.60090373597999	34.935043976438315	20.338094085370773
12	20.01936577099976	20.36633583474542	39.87331558137658	19.740982812878237
13	21.286209957233922	19.54329056725571	41.07560719761155	18.094892277898815
14	18.445896877269426	23.218752521584765	39.6554506576293	18.6798999435165
15	16.64245945291697	22.657952069716774	39.90559186637618	20.793996610990074
16	17.804405712902444	21.911562979101106	40.45428871136932	19.829742596627128
17	18.171548454772857	22.214153150972322	36.85144839828936	22.762849995965464
18	19.04300814976196	21.709836197853626	38.50157346889373	20.74558218349068
19	19.385943677882675	24.195110142822564	37.07334785766158	19.34559832163318
20	21.532316630355847	25.14322601468571	35.17308157831034	18.151375776648106
21	20.82223836036472	25.635439360929556	35.97998870330025	17.56233357540547
22	18.85741951101428	24.50173485031873	35.05204550956185	21.58880012910514
23	18.740417977890743	25.151295085935608	33.99096264020011	22.117324295973535
24	19.498910675381264	25.163398692810457	34.539659485193255	20.798031146615024
25	20.01936577099976	25.929960461550877	31.860727830226743	22.189945937222625
26	17.10239651416122	27.1120793996611	34.15637860082305	21.629145485354638
27	18.26030823852175	25.256193012184298	36.36326958767046	20.1202291616235
28	17.800371177277498	25.211813120309852	35.35463568143307	21.633180020979587
29	18.562898410392965	24.65101266844186	35.08835633018639	21.697732590978777
30	22.456225288469298	24.154764786573065	34.60421205519245	18.784797869765193
31	20.192850802872588	27.382393286532718	32.08262728959897	20.342128620995723
32	19.837811667877027	26.30920681029614	31.719519083353504	22.13346243847333
33	22.44412168159445	24.723634309690954	30.339707899620755	22.492536109093844
34	19.886226095376422	25.461954329056724	31.816347938352298	22.835471637214557
35	19.511014282256113	26.13168724279835	31.52586137335593	22.831437101589607
36	20.798031146615024	27.709190672153632	32.43766642459453	19.055111756636812
37	20.241265230371987	27.39853142903252	32.849189058339384	19.511014282256113
38	19.878157024126523	28.407165335269912	29.803114661502462	21.911562979101106
39	23.420479302832245	26.402001129669976	27.535705640280806	22.641813927216976
40	22.359396433470508	26.55934801904301	29.117243605261034	21.96401194222545
41	18.59517469539256	29.73049302025337	28.80658436213992	22.867747922214154
42	21.435487775357057	29.16162349713548	29.661905914629227	19.740982812878237
43	21.21358831598483	26.184136205922698	31.977729363350278	20.62454611474219
44	21.4879367384814	25.88154603405148	30.392156862745097	22.23836036472202
45	22.04470265472444	25.014120874687322	28.68151375776648	24.259662712821754
46	21.330589849108367	26.607762446542406	30.896473815863796	21.165173888485437
47	19.43435810538207	29.197934317760026	31.654966513354317	19.712741063503593
48	18.546760267893163	27.882675704026465	32.57484063584282	20.995723392237554
49	20.959412571613008	26.09537642217381	31.562172193980476	21.383038812232712
50	19.506979746631163	25.15936415718551	30.86016299523925	24.473493100944083
51	19.25683853788429	25.687888324053905	29.22214153150972	25.833131606552083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	98.0
2	125.0
3	601.0
4	1077.0
5	704.0
6	331.0
7	337.5
8	344.0
9	351.5
10	359.0
11	353.0
12	347.0
13	335.0
14	323.0
15	295.5
16	268.0
17	248.5
18	229.0
19	239.0
20	249.0
21	242.5
22	236.0
23	248.5
24	261.0
25	264.0
26	279.5
27	292.0
28	303.5
29	315.0
30	332.0
31	349.0
32	370.5
33	392.0
34	460.5
35	529.0
36	522.5
37	516.0
38	585.5
39	655.0
40	765.0
41	875.0
42	978.0
43	1081.0
44	1169.0
45	1257.0
46	1460.5
47	1664.0
48	1850.5
49	2037.0
50	2036.0
51	2035.0
52	1790.0
53	1545.0
54	1367.5
55	1190.0
56	1125.5
57	1061.0
58	1029.5
59	998.0
60	969.5
61	941.0
62	844.5
63	748.0
64	671.0
65	594.0
66	508.0
67	422.0
68	334.0
69	246.0
70	223.5
71	201.0
72	174.0
73	147.0
74	117.0
75	76.5
76	66.0
77	49.0
78	32.0
79	23.0
80	14.0
81	9.5
82	5.0
83	4.0
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	24786.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.39909626402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.92713050638122	68.93407568788832
2	8.95944833264718	14.048253046074397
3	1.729106628242075	4.066811909949164
4	0.514615067929189	1.6138142499798274
5	0.23157678056813505	0.907770515613653
6	0.13894606834088102	0.6535947712418301
7	0.11321531494442158	0.6213184862422335
8	0.06689995883079457	0.41959170499475507
9	0.08748456154796212	0.6172839506172839
>10	0.2006998764923837	2.9290728637133867
>50	0.01543845203787567	0.9037359799887034
>100	0.01029230135858378	1.4887436456063907
>500	0.00514615067929189	2.795933188090051
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	693	2.795933188090051	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	236	0.9521504074880982	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	133	0.5365932381182926	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	89	0.3590736706205116	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	78	0.31469377874606635	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.22996853062212538	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	46	0.18558863874768014	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	44	0.177519567497781	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	42	0.1694504962478819	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	33	0.13313967562333576	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	31	0.12507060437343662	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.12103606874848705	No Hit
TCCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	26	0.10489792624868877	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	25	0.10086339062373921	No Hit
TTTCCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	25	0.10086339062373921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34697006374566286	0.0
2	0.0	0.0	0.0	1.1175663681110304	0.0
3	0.0	0.0	0.0	1.6339869281045751	0.0
4	0.0	0.0	0.0	2.1947873799725652	0.0
5	0.0	0.0	0.0	3.6068748487049143	0.0
6	0.0	0.0	0.0	4.905995319938675	0.0
7	0.0	0.0	0.0	5.79359315742758	0.0
8	0.0	0.0	0.0	7.076575486161543	0.0
9	0.0	0.0	0.0	7.742273864278221	0.0
10	0.0	0.0	0.0	8.928427338013394	0.0
11	0.0	0.0	0.0	11.942225449850723	0.0
12	0.0	0.0	0.0	13.959493262325505	0.0
13	0.0	0.0	0.0	14.67764060356653	0.0
14	0.0	0.0	0.0	14.964092632937948	0.0
15	0.0	0.0	0.0	15.33526991043331	0.0
16	0.0	0.0	0.0	16.150246106673123	0.0
17	0.0	0.0	0.0	17.20325990478496	0.0
18	0.0	0.0	0.0	18.13120309852336	0.0
19	0.0	0.0	0.0	18.59920923101751	0.0
20	0.0	0.0	0.0	19.06318082788671	0.0
21	0.0	0.0	0.0	19.567497781005407	0.0
22	0.0	0.0	0.0	19.91850238037602	0.0
23	0.0	0.0	0.0	20.204954409747437	0.0
24	0.0	0.0	0.0	20.442992011619463	0.0
25	0.0	0.0	0.0	20.63664972161704	0.0
26	0.0	0.0	0.0	20.798031146615024	0.0
27	0.0	0.0	0.0	20.93520535786331	0.0
28	0.0	0.0	0.0	21.084483175986446	0.0
29	0.0	0.0	0.0	21.233760994109577	0.0
30	0.0	0.0	0.0	21.379004276607763	0.0
31	0.0	0.0	0.0	21.467764060356654	0.0
32	0.0	0.0	0.0	21.572661986605343	0.0
33	0.0	0.0	0.0	21.65335269910433	0.0
34	0.0	0.0	0.0	21.76631969660292	0.0
35	0.0	0.0	0.0	21.87525215847656	0.0
36	0.0	0.0	0.0	21.9841846203502	0.0
37	0.0	0.0	0.0	22.097151617848784	0.0
38	0.0	0.0	0.0	22.210118615347373	0.0
39	0.0	0.0	0.0	22.33518921972081	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	20	6.900203E-4	45.0	10
CGCTGAA	20	6.900203E-4	45.0	37
TTGTTGG	50	2.0008883E-11	45.0	2
GCGACCT	20	6.900203E-4	45.0	11
GCGACCC	20	6.900203E-4	45.0	31
CCGCTGA	20	6.900203E-4	45.0	36
GCGCGAC	20	6.900203E-4	45.0	9
GGCGACC	20	6.900203E-4	45.0	30
ACCCGCT	20	6.900203E-4	45.0	34
CTGAATT	20	6.900203E-4	45.0	39
CGGCGTT	20	6.900203E-4	45.0	43
TTGGGGT	20	6.900203E-4	45.0	5
TTGGGAC	20	6.900203E-4	45.0	5
CTGCGGG	20	6.900203E-4	45.0	3
TAACCCG	20	6.900203E-4	45.0	38
TCAGATC	20	6.900203E-4	45.0	17
GGCTTTG	25	3.7809343E-5	45.0	1
CTCAGAT	20	6.900203E-4	45.0	16
CCTCAGA	20	6.900203E-4	45.0	15
CAGATCA	20	6.900203E-4	45.0	18
>>END_MODULE