##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24563
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26861539714204	33.0	31.0	34.0	30.0	34.0
2	32.43211334120425	34.0	31.0	34.0	31.0	34.0
3	32.54744941578797	34.0	31.0	34.0	31.0	34.0
4	35.99014778325123	37.0	35.0	37.0	35.0	37.0
5	36.028253877783655	37.0	35.0	37.0	35.0	37.0
6	36.007938769694256	37.0	35.0	37.0	35.0	37.0
7	35.95077962789561	37.0	35.0	37.0	35.0	37.0
8	35.81150510931075	37.0	35.0	37.0	35.0	37.0
9	37.65468387411961	39.0	38.0	39.0	35.0	39.0
10	37.29959695476937	39.0	37.0	39.0	34.0	39.0
11	37.2246468265277	39.0	37.0	39.0	34.0	39.0
12	37.207995765989494	39.0	37.0	39.0	34.0	39.0
13	37.19386882709767	39.0	37.0	39.0	34.0	39.0
14	38.405447217359445	40.0	38.0	41.0	34.0	41.0
15	38.46280991735537	40.0	38.0	41.0	34.0	41.0
16	38.55583601351626	40.0	38.0	41.0	34.0	41.0
17	38.5188291332492	40.0	38.0	41.0	34.0	41.0
18	38.31999348613769	40.0	38.0	41.0	34.0	41.0
19	38.14794609778936	40.0	37.0	41.0	34.0	41.0
20	37.8423645320197	40.0	35.0	41.0	34.0	41.0
21	37.76863575296177	39.0	35.0	41.0	34.0	41.0
22	37.73936408419167	39.0	35.0	41.0	34.0	41.0
23	37.6477628954118	39.0	35.0	41.0	33.0	41.0
24	37.53865570166511	39.0	35.0	41.0	33.0	41.0
25	37.47184790131499	39.0	35.0	41.0	33.0	41.0
26	37.388674021902865	39.0	35.0	41.0	33.0	41.0
27	37.30602939380369	39.0	35.0	41.0	33.0	41.0
28	37.22973578145992	39.0	35.0	41.0	33.0	41.0
29	37.06603427920042	39.0	35.0	41.0	33.0	41.0
30	36.87285754997354	39.0	35.0	41.0	32.0	41.0
31	36.65688230265033	39.0	35.0	41.0	31.0	41.0
32	36.489353906281806	39.0	35.0	41.0	31.0	41.0
33	36.16545210275618	39.0	35.0	41.0	30.0	41.0
34	35.8410210479176	39.0	35.0	41.0	27.0	41.0
35	35.64096405162236	39.0	35.0	41.0	26.0	41.0
36	35.51577576028987	39.0	35.0	41.0	25.0	41.0
37	35.421121198550665	39.0	35.0	41.0	25.0	41.0
38	35.271017383870046	38.0	35.0	41.0	24.0	41.0
39	35.232870577698165	38.0	35.0	41.0	24.0	41.0
40	35.022147131865	38.0	35.0	41.0	23.0	41.0
41	34.904856898587305	38.0	35.0	40.0	23.0	41.0
42	34.75809143834222	38.0	35.0	40.0	22.0	41.0
43	34.652241175752145	38.0	34.0	40.0	22.0	41.0
44	34.51695639783414	38.0	34.0	40.0	21.0	41.0
45	34.48019378740382	38.0	34.0	40.0	22.0	41.0
46	34.303057444123276	37.0	34.0	40.0	20.0	41.0
47	34.22228555143916	37.0	34.0	40.0	20.0	41.0
48	34.10300044782804	37.0	34.0	40.0	20.0	41.0
49	34.0785734641534	37.0	34.0	40.0	20.0	41.0
50	33.90766600170989	36.0	33.0	40.0	20.0	41.0
51	33.12221634165208	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	1.0
15	2.0
16	5.0
17	5.0
18	9.0
19	13.0
20	24.0
21	40.0
22	80.0
23	99.0
24	163.0
25	275.0
26	456.0
27	485.0
28	490.0
29	477.0
30	477.0
31	545.0
32	610.0
33	842.0
34	1440.0
35	1769.0
36	2465.0
37	3008.0
38	4891.0
39	5887.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.0432357611041	5.158164719293246	40.05211089850588	28.746488621096773
2	34.18556365264829	5.67113137646053	43.598094695273375	16.5452102756178
3	21.279973944550747	5.610063917273949	54.724585759068525	18.385376379106784
4	17.26173513007369	6.074176607091967	53.90628180596833	22.757806456866017
5	15.568130928632495	6.87619590440907	53.74750641208321	23.808166754875216
6	19.45609249684485	7.820705939828197	57.313846028579576	15.409355534747386
7	69.29935268493263	3.896103896103896	22.130847209217112	4.673696209746367
8	69.09172332369825	4.734763668932948	20.933925009160117	5.2395879982086875
9	63.10304115946749	6.4609371819403165	23.494687131050767	6.941334527541423
10	31.742865285185033	22.25298212759028	29.601433049708913	16.402719537515775
11	23.38476570451492	21.662663355453322	34.87359035948378	20.078980580547977
12	20.559377926149086	20.335463909131622	39.795627569922246	19.309530594797053
13	21.455033994218947	20.160403859463422	40.25974025974026	18.124821886577372
14	18.686642511093922	22.8921548670765	39.77527175019338	18.645930871636203
15	17.22916581850751	22.497251964336602	39.811912225705335	20.461669991450556
16	18.56450759272076	21.817367585392663	39.85262386516305	19.76550095672353
17	18.08411024711965	22.277409111264912	37.10458820176689	22.53389243984855
18	19.020477954647234	21.605667060212514	39.05060456784595	20.323250417294307
19	19.785856776452388	24.349631559662907	36.55905223303343	19.30545943085128
20	21.084558075153687	25.64833285836421	35.374343524813746	17.89276554166836
21	20.901355697593942	25.72568497333388	36.001302772462644	17.371656556609537
22	19.525302283922976	23.681960672556286	35.305133737735616	21.487603305785125
23	18.588934576395392	25.32671090664821	33.63188535602329	22.452469160933113
24	19.781785612506617	25.15572202092578	34.7392419492733	20.323250417294307
25	19.65965069413345	26.07987623661605	32.341326385213534	21.919146684036967
26	17.778772951186745	27.113951878842162	33.876155192769616	21.23111997720148
27	18.755852298172044	24.748605626348574	36.23743028131743	20.25811179416195
28	17.34315840898913	25.929243170622478	35.337703049301794	21.389895371086592
29	18.654073199527748	24.540976265114196	35.203354639091316	21.601595896266744
30	22.118633717379797	24.43512600252412	34.69445914586981	18.751781134226274
31	20.19704433497537	27.109880714896388	32.12555469608761	20.56752025404063
32	19.912062858771325	26.393355860440497	31.661442006269592	22.033139274518586
33	22.090135569759394	24.418841346741033	31.278752595367017	22.212270488132557
34	20.33139274518585	25.530269103936813	31.78357692464275	22.354761226234583
35	19.338028742417457	26.275292106013108	32.17440866343688	22.212270488132557
36	20.579733745877945	28.33530106257379	32.04413141717217	19.040833774376097
37	19.96498799006636	27.87525953670154	32.691446484549935	19.468305988682165
38	20.237755974433092	28.396368521760373	29.296095753776	22.069779750030534
39	23.34812522900297	26.975532304685913	27.252371452998414	22.42397101331271
40	22.167487684729064	27.077311403330214	28.998900785734644	21.75630012620608
41	19.061189594104956	29.809062410943287	29.27166877010137	21.858079224850385
42	21.426535846598544	29.340878557179494	29.28795342588446	19.9446321703375
43	21.060131091479054	26.23050930260962	32.47160363147824	20.237755974433092
44	21.271831616659203	26.09616089239914	29.670642836787035	22.96136465415462
45	22.187843504457923	25.22493180800391	29.186174327240156	23.401050360298008
46	21.121198550665635	27.28901192851036	30.171396002117007	21.418393518707
47	18.902414200219845	30.037047591906525	31.486381956601395	19.57415625127224
48	18.686642511093922	28.35158571835688	32.39425151650857	20.56752025404063
49	20.750722631600375	26.495134959084805	31.767292268859666	20.986850140455157
50	19.47237715262794	25.705329153605017	30.407523510971785	24.414770182795262
51	19.59858323494687	26.075805072670278	29.10475104832472	25.220860644058135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	96.0
2	127.0
3	620.5
4	1114.0
5	712.0
6	310.0
7	326.0
8	342.0
9	343.0
10	344.0
11	343.0
12	342.0
13	345.0
14	348.0
15	310.0
16	272.0
17	250.5
18	229.0
19	236.0
20	243.0
21	241.5
22	240.0
23	238.5
24	237.0
25	254.0
26	304.5
27	338.0
28	343.0
29	348.0
30	350.5
31	353.0
32	383.5
33	414.0
34	451.5
35	489.0
36	505.0
37	521.0
38	593.5
39	666.0
40	755.0
41	844.0
42	928.0
43	1012.0
44	1096.0
45	1180.0
46	1357.5
47	1535.0
48	1766.5
49	1998.0
50	2031.5
51	2065.0
52	1828.0
53	1591.0
54	1379.5
55	1168.0
56	1094.0
57	1020.0
58	988.0
59	956.0
60	924.0
61	892.0
62	826.5
63	761.0
64	678.0
65	595.0
66	510.5
67	426.0
68	369.5
69	313.0
70	270.0
71	227.0
72	193.0
73	159.0
74	123.0
75	70.0
76	53.0
77	46.0
78	39.0
79	28.5
80	18.0
81	12.0
82	6.0
83	4.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	24563.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.01722102349062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98495543304654	69.52326670195009
2	8.903086197125045	14.069942596588364
3	1.7363078983976505	4.115946749175589
4	0.5152248956669586	1.6284655783088384
5	0.25246019887680976	0.9974351667141637
6	0.1597197176567572	0.7572364939136098
7	0.09274048122005256	0.5129666571672842
8	0.08243598330671338	0.5211089850588283
9	0.05152248956669587	0.36640475511948867
>10	0.1906332113967747	2.691039368155356
>50	0.020608995826678345	1.1602817245450474
>100	0.005152248956669586	0.9078695599071774
>500	0.005152248956669586	2.748035663396165
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	2.748035663396165	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	223	0.9078695599071774	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	93	0.3786182469568049	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	69	0.2809103122582746	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	69	0.2809103122582746	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	54	0.21984285307169318	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49	0.19948703334283271	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	42	0.17098888572242804	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	39	0.15877539388511178	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	32	0.13027724626470708	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	27	0.10992142653584659	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	25	0.1017790986443024	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28905264014981885	0.0
2	0.0	0.0	0.0	0.8997272320156333	0.0
3	0.0	0.0	0.0	1.3434841021047919	0.0
4	0.0	0.0	0.0	1.8849489068924805	0.0
5	0.0	0.0	0.0	3.179579041648007	0.0
6	0.0	0.0	0.0	4.262508651223385	0.0
7	0.0	0.0	0.0	5.027887473028539	0.0
8	0.0	0.0	0.0	6.155599886007409	0.0
9	0.0	0.0	0.0	6.754060986035908	0.0
10	0.0	0.0	0.0	7.991694825550625	0.0
11	0.0	0.0	0.0	10.83743842364532	0.0
12	0.0	0.0	0.0	12.693889182917395	0.0
13	0.0	0.0	0.0	13.385987053698653	0.0
14	0.0	0.0	0.0	13.609901070716118	0.0
15	0.0	0.0	0.0	14.017017465293327	0.0
16	0.0	0.0	0.0	14.725399991857673	0.0
17	0.0	0.0	0.0	15.84904124089077	0.0
18	0.0	0.0	0.0	16.630704718479013	0.0
19	0.0	0.0	0.0	17.16402719537516	0.0
20	0.0	0.0	0.0	17.567072426006597	0.0
21	0.0	0.0	0.0	17.9456906729634	0.0
22	0.0	0.0	0.0	18.287668444408258	0.0
23	0.0	0.0	0.0	18.62964621585311	0.0
24	0.0	0.0	0.0	18.85763139681635	0.0
25	0.0	0.0	0.0	18.987908643081056	0.0
26	0.0	0.0	0.0	19.114114725399993	0.0
27	0.0	0.0	0.0	19.24846313561047	0.0
28	0.0	0.0	0.0	19.370598053983635	0.0
29	0.0	0.0	0.0	19.48051948051948	0.0
30	0.0	0.0	0.0	19.610796726784187	0.0
31	0.004071163945772096	0.0	0.0	19.74921630094044	0.0
32	0.004071163945772096	0.0	0.0	19.903920530879777	0.0
33	0.004071163945772096	0.0	0.0	20.03826894109026	0.0
34	0.004071163945772096	0.0	0.0	20.127834547897244	0.0
35	0.004071163945772096	0.0	0.0	20.245898302324633	0.0
36	0.004071163945772096	0.0	0.0	20.384317876480885	0.0
37	0.004071163945772096	0.0	0.0	20.49016813907096	0.0
38	0.004071163945772096	0.0	0.0	20.624516549281438	0.0
39	0.004071163945772096	0.0	0.0	20.783291943166553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTGG	20	6.8991014E-4	45.0	2
CTCCTGG	20	6.8991014E-4	45.0	2
TGTTTGG	35	1.1483098E-7	45.0	2
TTCCTGG	25	3.7800295E-5	45.0	2
TTGGGCG	25	3.7800295E-5	45.0	5
TTGGGCA	25	3.7800295E-5	45.0	5
GCTCGGG	25	3.7800295E-5	45.0	3
CGGGACT	25	3.7800295E-5	45.0	6
TTTAGGG	20	6.8991014E-4	45.0	3
CTCGGGA	25	3.7800295E-5	45.0	4
GGGAACA	25	3.7800295E-5	45.0	7
TGGGATT	25	3.7800295E-5	45.0	6
TGGGAGC	25	3.7800295E-5	45.0	6
TGGGAAC	25	3.7800295E-5	45.0	6
GGAACAT	20	6.8991014E-4	45.0	8
CGTTTTT	260	0.0	44.134617	1
TGGGCTT	40	3.2777098E-7	39.375	6
TCTTTTG	40	3.2777098E-7	39.375	1
GGGGATC	40	3.2777098E-7	39.375	7
TTTGGGA	75	0.0	39.000004	4
>>END_MODULE