SRR2933610.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933610.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146542
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3990	2.7227688990187113	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	788	0.5377297976006878	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	771	0.5261290278554953	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	677	0.4619835951467839	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	481	0.32823354396691734	RNA PCR Primer, Index 33 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	460	0.31390318134050305	RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	326	0.2224618198195739	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	239	0.16309317465300052	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.1596811835514733	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	215	0.1467156173656699	Illumina PCR Primer Index 5 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	195	0.13306765295956108	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	159	0.10850131702856518	RNA PCR Primer, Index 33 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	158	0.10781891880825975	Illumina PCR Primer Index 5 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	147	0.1003125383848999	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGG	20	7.013128E-4	45.0	2
ATGGGAC	20	7.013128E-4	45.0	5
ATGGGAA	20	7.013128E-4	45.0	5
TCTGAGG	20	7.013128E-4	45.0	2
CCCTCGT	20	7.013128E-4	45.0	14
GGGCTTA	20	7.013128E-4	45.0	7
GAAGACC	25	3.8741044E-5	45.0	30
GTCCTTG	20	7.013128E-4	45.0	1
GGGTATT	20	7.013128E-4	45.0	7
GTGGGCA	20	7.013128E-4	45.0	5
GAATATA	45	3.8016879E-10	45.0	15
GATCAAC	40	6.7448127E-9	45.0	9
GTAGGGC	25	3.8741044E-5	45.0	4
GCGTTTG	20	7.013128E-4	45.0	1
GCACCGA	20	7.013128E-4	45.0	9
GGCTTAG	20	7.013128E-4	45.0	1
ATATACC	20	7.013128E-4	45.0	17
CTACGAA	50	2.1827873E-11	45.0	11
TACGAAT	45	3.8016879E-10	45.0	12
GCTTAGG	20	7.013128E-4	45.0	2