##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933610.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146542
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.915894419347353	33.0	31.0	34.0	30.0	34.0
2	32.08986502163202	33.0	31.0	34.0	30.0	34.0
3	32.24990787624026	33.0	31.0	34.0	30.0	34.0
4	35.812975119760885	37.0	35.0	37.0	35.0	37.0
5	35.8362380750911	37.0	35.0	37.0	35.0	37.0
6	35.80404935103929	37.0	35.0	37.0	35.0	37.0
7	35.855099561900346	37.0	35.0	37.0	35.0	37.0
8	35.6349374240832	37.0	35.0	37.0	33.0	37.0
9	37.42357139932579	39.0	37.0	39.0	34.0	39.0
10	36.901871135920075	39.0	37.0	39.0	32.0	39.0
11	36.95764354246564	39.0	37.0	39.0	33.0	39.0
12	37.10377229736185	39.0	37.0	39.0	34.0	39.0
13	37.056127253620126	39.0	37.0	39.0	33.0	39.0
14	38.07318720912776	40.0	37.0	41.0	33.0	41.0
15	38.08726508441266	40.0	37.0	41.0	33.0	41.0
16	38.17300842079404	40.0	37.0	41.0	33.0	41.0
17	38.175560590137984	40.0	37.0	41.0	33.0	41.0
18	38.025146374418256	39.0	37.0	40.0	33.0	41.0
19	37.831072320563386	39.0	37.0	41.0	34.0	41.0
20	37.48022409957555	39.0	35.0	40.0	33.0	41.0
21	37.341963396159464	39.0	35.0	40.0	33.0	41.0
22	37.369075077452194	39.0	35.0	40.0	33.0	41.0
23	37.349694967995525	39.0	35.0	40.0	33.0	41.0
24	37.26326240941164	39.0	35.0	40.0	33.0	41.0
25	37.16340025385214	39.0	35.0	40.0	33.0	41.0
26	37.05211475208473	39.0	35.0	40.0	32.0	41.0
27	37.000620982380475	39.0	35.0	40.0	32.0	41.0
28	36.890277190157086	39.0	35.0	40.0	32.0	41.0
29	36.7553534140383	39.0	35.0	40.0	31.0	41.0
30	36.69777947619112	38.0	35.0	40.0	31.0	41.0
31	36.383500975829456	38.0	35.0	40.0	31.0	41.0
32	35.942016623220645	38.0	35.0	40.0	30.0	41.0
33	35.69316646422186	38.0	35.0	40.0	29.0	41.0
34	35.46584596907371	38.0	35.0	40.0	26.0	41.0
35	35.07739078216484	38.0	35.0	40.0	25.0	41.0
36	35.03851455555404	38.0	35.0	40.0	24.0	41.0
37	34.85437621978682	38.0	34.0	40.0	23.0	41.0
38	34.43831802486659	38.0	33.0	40.0	23.0	41.0
39	34.26034174502873	38.0	33.0	40.0	22.0	41.0
40	34.273914645630605	38.0	33.0	40.0	22.0	41.0
41	34.36565626236847	38.0	33.0	40.0	22.0	41.0
42	34.50225191412701	38.0	34.0	40.0	22.0	41.0
43	34.4436953228426	38.0	34.0	40.0	21.0	41.0
44	34.25458230404935	38.0	33.0	40.0	21.0	41.0
45	34.23171513968691	38.0	33.0	40.0	20.0	41.0
46	34.000034119911014	37.0	33.0	40.0	20.0	41.0
47	33.82882723041858	37.0	33.0	40.0	20.0	41.0
48	33.859780813691636	37.0	33.0	40.0	20.0	41.0
49	34.00779981165809	38.0	33.0	40.0	20.0	41.0
50	33.98563551746257	38.0	33.0	40.0	20.0	41.0
51	32.85698980497059	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	2.0
13	7.0
14	7.0
15	6.0
16	6.0
17	21.0
18	36.0
19	84.0
20	132.0
21	217.0
22	363.0
23	582.0
24	976.0
25	1784.0
26	2648.0
27	3002.0
28	3017.0
29	2935.0
30	3384.0
31	3803.0
32	4682.0
33	5997.0
34	8861.0
35	11960.0
36	16427.0
37	22677.0
38	33417.0
39	19498.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.633210956585824	5.504906443203996	42.81844112950554	27.043441470704643
2	32.85065032550395	5.777183333105867	42.57277777019557	18.799388571194605
3	20.58931910305578	5.34863725075405	53.21136602475741	20.850677621432766
4	15.964706364045803	6.014657913772161	54.06436380013921	23.956271922042827
5	13.600878928907752	6.855372521188465	54.681251791295324	24.862496758608454
6	17.51170312947824	7.741125411144928	59.751470568164756	14.995700891212078
7	68.16339342986993	2.9854922138363063	22.935404184465888	5.915710171827872
8	66.1155163707333	3.6883623807509114	22.392215201102754	7.803906047413029
9	59.821757584856215	5.902744605642068	25.80420630262996	8.47129150687175
10	28.53106959097051	21.234867819464725	31.892563224195115	18.341499365369653
11	18.151792660124745	18.411786382061116	37.21185735147603	26.224563606338116
12	17.07428587026245	15.126038951290417	44.542861432217386	23.25681374622975
13	21.41092656030353	16.847729661121043	45.39927119870072	16.34207257987471
14	18.785740606788497	18.75366789043414	43.57180876472274	18.88878273805462
15	12.68237092437663	18.58170353891717	45.468193418951564	23.267732117754637
16	11.767957309167338	18.323074613421408	43.38346685591844	26.525501221492814
17	11.653996806376329	18.972035320931884	40.99780267773062	28.37616519496117
18	13.173015244776243	17.235331850254536	45.403365588022545	24.188287316946678
19	13.525132726453851	21.004217221001486	45.65244093843403	19.81820911411063
20	14.16453985888005	24.52334484311665	42.06575589250863	19.24635940549467
21	12.978531751989191	26.35967845395859	41.94087701819274	18.72091277585948
22	11.125138185639612	24.622974983281242	39.26655839281571	24.985328438263434
23	11.468384490453248	24.904123050047087	36.2803837807591	27.347108678740568
24	13.306765295956108	23.93784717009458	37.050811371483945	25.70457616246537
25	12.356184575070628	26.964283277149214	33.63950266817704	27.040029479603117
26	12.040234199069209	28.469653751143014	35.97671657272318	23.5133954770646
27	15.261836197131199	25.851974178051346	37.87378362517231	21.012405999645154
28	13.367498737563293	22.557355570416675	38.15766128481937	25.917484407200664
29	15.581198564234144	21.47370719657163	37.04808177860272	25.8970124605915
30	24.218312838640117	23.703784580529813	32.700522717036755	19.377379863793315
31	22.471373394658187	30.11423346207913	27.701273355079092	19.71311978818359
32	19.537743445565095	28.444405016991713	28.707810730029614	23.310040807413575
33	24.95871490767152	21.67910906088357	27.89302725498492	25.469148776459992
34	22.740920691678838	22.629007383548743	28.709175526470226	25.920896398302197
35	20.32727818645849	26.764340598599716	29.002606761201566	23.905774453740225
36	28.275170258355963	27.050265452907702	26.41972949734547	18.254834791390863
37	29.51167583354943	23.855959383657925	29.031949884674702	17.600414898117947
38	26.283932251504687	20.279510311037107	33.954770645958156	19.481786791500046
39	26.883077888932867	19.91101527207217	31.837971366570677	21.36793547242429
40	25.286948451638438	25.90247164635395	30.430866236300858	18.37971366570676
41	19.529554666921427	27.145118805530156	31.935554312074355	21.38977221547406
42	21.60677484953119	21.590397292243864	32.71690027432408	24.08592758390086
43	22.097419169930806	17.8078639570908	36.0046948997557	24.090021973222694
44	23.239753790722116	18.162028633429326	32.43438741111763	26.16383016473093
45	26.82916842952874	18.391314435451953	29.764163175062443	25.015353959956872
46	26.55416194674564	20.530632856109513	34.47953487737304	18.435670319771805
47	18.907207490002868	24.784703361493634	36.87952941818728	19.428559730316223
48	17.479630413123886	21.37953624216948	39.08777005909568	22.053063285610953
49	23.859371374759455	16.20627533403393	37.64449782314968	22.289855468056942
50	22.421558324575887	14.705681647582264	33.89335480613067	28.97940522171118
51	18.669050511116268	14.892658759945954	31.333679081764952	35.104611647172824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	571.5
2	884.0
3	3634.5
4	6385.0
5	4054.5
6	1724.0
7	1643.0
8	1562.0
9	1557.5
10	1553.0
11	1467.0
12	1381.0
13	1276.5
14	1172.0
15	1126.5
16	1081.0
17	991.5
18	902.0
19	891.5
20	881.0
21	803.5
22	726.0
23	741.0
24	756.0
25	773.5
26	823.0
27	855.0
28	859.0
29	863.0
30	998.5
31	1134.0
32	1230.0
33	1326.0
34	1631.0
35	1936.0
36	2020.5
37	2105.0
38	2325.0
39	2545.0
40	3133.5
41	3722.0
42	4860.0
43	5998.0
44	7332.5
45	8667.0
46	11863.0
47	15059.0
48	18854.5
49	22650.0
50	22934.0
51	23218.0
52	19107.5
53	14997.0
54	11070.0
55	7143.0
56	5318.0
57	3493.0
58	2979.5
59	2466.0
60	2222.0
61	1978.0
62	1850.0
63	1722.0
64	1599.5
65	1477.0
66	1244.0
67	1011.0
68	839.5
69	668.0
70	576.0
71	484.0
72	434.0
73	384.0
74	318.5
75	191.5
76	130.0
77	118.5
78	107.0
79	79.0
80	51.0
81	39.0
82	27.0
83	16.0
84	5.0
85	4.5
86	4.0
87	5.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	146542.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.930040534454285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77309940093458	42.9392256144996
2	12.30463269115799	13.763972103560754
3	4.854747989897634	8.145787555786054
4	2.131501567818841	4.768598763494425
5	1.1603079513427117	3.244803537552374
6	0.6771513280706677	2.27238607361712
7	0.3977501494613292	1.5572327387370175
8	0.27818108612632836	1.2446943538371253
9	0.2110759995607667	1.0624940290155724
>10	1.1115042520223033	11.241828281311843
>50	0.06710508656556168	2.6511170858866397
>100	0.02806212710923488	2.8592485430797994
>500	0.003660277449030637	1.525842420602967
>1k	0.0012200924830102122	2.7227688990187113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3990	2.7227688990187113	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	788	0.5377297976006878	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	771	0.5261290278554953	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	677	0.4619835951467839	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	481	0.32823354396691734	RNA PCR Primer, Index 33 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	460	0.31390318134050305	RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	326	0.2224618198195739	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	239	0.16309317465300052	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.1596811835514733	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	215	0.1467156173656699	Illumina PCR Primer Index 5 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	195	0.13306765295956108	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	159	0.10850131702856518	RNA PCR Primer, Index 33 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	158	0.10781891880825975	Illumina PCR Primer Index 5 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	147	0.1003125383848999	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5622961335316837	0.0
2	0.0	0.0	0.0	1.8711359200775204	0.0
3	0.0	0.0	0.0	2.6135851837698407	0.0
4	0.0	0.0	0.0	3.809146865744974	0.0
5	0.0	0.0	0.0	6.461628748072225	0.0
6	0.0	0.0	0.0	8.95033505752617	0.0
7	0.0	0.0	0.0	11.04461519564357	0.0
8	0.0	0.0	0.0	14.055356143631178	0.0
9	0.0	0.0	0.0	15.517735529745739	0.0
10	0.0	0.0	0.0	18.727054359842228	0.0
11	0.0	0.0	0.0	26.911056215965388	0.0
12	0.0	0.0	0.0	33.99162014985465	0.0
13	0.0	0.0	0.0	36.24558147152352	0.0
14	0.0	0.0	0.0	37.031704221315394	0.0
15	0.0	0.0	0.0	38.17540363854731	0.0
16	0.0	0.0	0.0	40.93570443968282	0.0
17	0.0	0.0	0.0	44.175048791472754	0.0
18	0.0	0.0	0.0	46.98038787514842	0.0
19	0.0	0.0	0.0	48.63520355938912	0.0
20	0.0	0.0	0.0	49.92834818686793	0.0
21	0.0	0.0	0.0	51.53880798678877	0.0
22	0.0	0.0	0.0	52.923393975788514	0.0
23	0.0	0.0	0.0	53.98657040302439	0.0
24	0.0	0.0	0.0	54.54272495257332	0.0
25	0.0	0.0	0.0	54.92145596484284	0.0
26	0.0	0.0	0.0	55.18690887254166	0.0
27	0.0	0.0	0.0	55.43120743541101	0.0
28	0.0	0.0	0.0	55.62637332641837	0.0
29	0.0	0.0	0.0	55.82563360674755	0.0
30	0.0	0.0	0.0	56.05355461232957	0.0
31	0.0	0.0	0.0	56.23166054782929	0.0
32	0.0	0.0	0.0	56.40430729756657	0.0
33	0.0	0.0	0.0	56.56740047221957	0.0
34	0.0	0.0	0.0	56.71821047890707	0.0
35	0.0	0.0	0.0	56.874479671357015	0.0
36	0.0	0.0	0.0	57.0225600851633	0.0
37	0.0	0.0	0.0	57.15562773812286	0.0
38	0.0	0.0	0.0	57.314626523454024	0.0
39	0.0	0.0	0.0	57.47499010522581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGG	20	7.013128E-4	45.0	2
ATGGGAC	20	7.013128E-4	45.0	5
ATGGGAA	20	7.013128E-4	45.0	5
TCTGAGG	20	7.013128E-4	45.0	2
CCCTCGT	20	7.013128E-4	45.0	14
GGGCTTA	20	7.013128E-4	45.0	7
GAAGACC	25	3.8741044E-5	45.0	30
GTCCTTG	20	7.013128E-4	45.0	1
GGGTATT	20	7.013128E-4	45.0	7
GTGGGCA	20	7.013128E-4	45.0	5
GAATATA	45	3.8016879E-10	45.0	15
GATCAAC	40	6.7448127E-9	45.0	9
GTAGGGC	25	3.8741044E-5	45.0	4
GCGTTTG	20	7.013128E-4	45.0	1
GCACCGA	20	7.013128E-4	45.0	9
GGCTTAG	20	7.013128E-4	45.0	1
ATATACC	20	7.013128E-4	45.0	17
CTACGAA	50	2.1827873E-11	45.0	11
TACGAAT	45	3.8016879E-10	45.0	12
GCTTAGG	20	7.013128E-4	45.0	2
>>END_MODULE