SRR2933609.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145743
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3854	2.6443808622026443	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	832	0.5708678976005709	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	768	0.526954982400527	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	661	0.4535380773004536	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	483	0.3314052819003314	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	457	0.31356566010031356	RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	336	0.23054280480023054	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	275	0.1886883075001887	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.1578120390001578	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	193	0.13242488490013243	Illumina PCR Primer Index 5 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	180	0.1235050740001235	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	172	0.11801595960011801	RNA PCR Primer, Index 33 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	172	0.11801595960011801	Illumina PCR Primer Index 5 (95% over 24bp)
GTGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	153	0.104979312900105	RNA PCR Primer, Index 33 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	151	0.10360703430010361	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTGG	30	2.1524265E-6	45.000004	2
TTGTAGG	30	2.1524265E-6	45.000004	2
GAATATA	30	2.1524265E-6	45.000004	15
GTAGGGA	30	2.1524265E-6	45.000004	4
TGGCTTG	30	2.1524265E-6	45.000004	1
CATGGGC	40	6.7448127E-9	45.0	4
CTAGAAT	20	7.013E-4	45.0	41
CGGGTAT	20	7.013E-4	45.0	6
CTCACAA	20	7.013E-4	45.0	32
CTCGTCC	20	7.013E-4	45.0	37
CTACTCA	20	7.013E-4	45.0	29
GCGCGAC	25	3.874E-5	45.0	9
GGCACCG	20	7.013E-4	45.0	8
CAGCATA	20	7.013E-4	45.0	38
GGGTATT	25	3.874E-5	45.0	7
GGGTATG	55	1.8189894E-12	45.0	7
GTGGGAC	20	7.013E-4	45.0	5
TCTTAGG	20	7.013E-4	45.0	2
ATTAATC	20	7.013E-4	45.0	19
TTCGTTG	45	3.8016879E-10	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content