##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145743
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81188804951181	33.0	31.0	34.0	30.0	34.0
2	32.01945891054802	33.0	31.0	34.0	30.0	34.0
3	32.18994394241919	33.0	31.0	34.0	30.0	34.0
4	35.74289674289674	37.0	35.0	37.0	35.0	37.0
5	35.83070885051083	37.0	35.0	37.0	35.0	37.0
6	35.81297214960581	37.0	35.0	37.0	35.0	37.0
7	35.79404842771179	37.0	35.0	37.0	35.0	37.0
8	35.605991368367604	37.0	35.0	37.0	33.0	37.0
9	37.41245205601641	39.0	37.0	39.0	34.0	39.0
10	36.947414284047944	39.0	37.0	39.0	32.0	39.0
11	36.98476770753999	39.0	37.0	39.0	33.0	39.0
12	37.095510590560096	39.0	37.0	39.0	33.0	39.0
13	37.08045669431808	39.0	37.0	39.0	33.0	39.0
14	38.13269247922713	40.0	37.0	41.0	33.0	41.0
15	38.122592508731124	40.0	37.0	41.0	33.0	41.0
16	38.3035274421413	40.0	38.0	41.0	34.0	41.0
17	38.36048386543436	40.0	38.0	41.0	34.0	41.0
18	38.25477724487625	39.0	38.0	41.0	34.0	41.0
19	38.08969899068909	39.0	37.0	41.0	34.0	41.0
20	37.704479803489704	39.0	35.0	41.0	34.0	41.0
21	37.61342225698662	39.0	35.0	40.0	33.0	41.0
22	37.615316001454616	39.0	35.0	40.0	33.0	41.0
23	37.54389576171754	39.0	35.0	40.0	33.0	41.0
24	37.45658453579246	39.0	35.0	41.0	33.0	41.0
25	37.36993200359537	39.0	35.0	40.0	33.0	41.0
26	37.32687676252033	39.0	35.0	40.0	33.0	41.0
27	37.280802508525284	39.0	35.0	40.0	33.0	41.0
28	37.231393617532234	39.0	35.0	40.0	33.0	41.0
29	37.02476962873003	39.0	35.0	40.0	32.0	41.0
30	36.873482774472876	39.0	35.0	40.0	32.0	41.0
31	36.547470547470546	39.0	35.0	40.0	31.0	41.0
32	36.22088196345622	38.0	35.0	40.0	30.0	41.0
33	35.86389740845186	38.0	35.0	40.0	29.0	41.0
34	35.64346829693364	38.0	35.0	40.0	27.0	41.0
35	35.348744022011346	38.0	35.0	40.0	25.0	41.0
36	35.26161805369726	38.0	35.0	40.0	25.0	41.0
37	35.113857955442114	38.0	35.0	40.0	24.0	41.0
38	34.656662755672656	38.0	34.0	40.0	23.0	41.0
39	34.50816162697351	38.0	33.0	40.0	23.0	41.0
40	34.56120705625656	38.0	34.0	40.0	23.0	41.0
41	34.67916812471268	38.0	34.0	40.0	23.0	41.0
42	34.727122400389725	38.0	34.0	40.0	22.0	41.0
43	34.64134812649664	38.0	34.0	40.0	22.0	41.0
44	34.46789897284947	38.0	34.0	40.0	21.0	41.0
45	34.417323645046416	38.0	34.0	40.0	21.0	41.0
46	34.18560068065018	38.0	33.0	40.0	20.0	41.0
47	34.134188262901134	38.0	33.0	40.0	20.0	41.0
48	34.115621333443116	38.0	33.0	40.0	20.0	41.0
49	34.26041730992226	38.0	34.0	40.0	20.0	41.0
50	34.11659565124911	38.0	33.0	40.0	20.0	41.0
51	33.15392849056215	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	4.0
12	1.0
13	4.0
14	8.0
15	5.0
16	15.0
17	23.0
18	34.0
19	76.0
20	126.0
21	188.0
22	332.0
23	608.0
24	990.0
25	1735.0
26	2566.0
27	2838.0
28	2843.0
29	2897.0
30	3066.0
31	3653.0
32	4327.0
33	5686.0
34	8425.0
35	11294.0
36	15164.0
37	20817.0
38	34358.0
39	23639.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.865688232024866	5.792387970605793	42.289509616242285	27.052414181127055
2	32.6019088395326	5.8349286072058355	42.66688623124267	18.896276322018895
3	20.795509904420793	5.383448947805383	52.68383387195268	21.137207275821137
4	16.07144082391607	6.0737050836060735	53.67530516045368	24.17954893202418
5	13.671325552513672	6.818852363406819	54.55699416095455	24.952827923124953
6	17.755912805417758	7.775330547607775	59.54179617545954	14.926960471514928
7	67.997090769368	3.062925835203063	22.864906033222866	6.075077362206075
8	66.0889373760661	3.6996631056036997	22.31050547882231	7.900894039507901
9	59.88280740755988	5.9083455123059085	25.498994805925502	8.70985227420871
10	28.542022601428542	21.172200380121172	31.867739788531868	18.41803722991842
11	18.374124314718372	18.424212483618422	37.0974935331371	26.104169668526104
12	17.37304707601737	15.087517067715087	44.17158971614417	23.36784614012337
13	21.21954399182122	16.92911494891693	45.22687195954523	16.624469099716624
14	19.041737853619043	18.991649684718993	42.95918157304296	19.007430888619005
15	12.614671030512614	18.833837645718834	45.363413680245365	23.18807764352319
16	11.842764318011843	18.28835690221829	42.88850922514289	26.980369554626982
17	11.875699004411876	18.871575307218873	41.03318855794103	28.21953713042822
18	13.250722161613252	17.353835175617355	45.00730738354501	24.388135279224386
19	13.715924607013715	20.899803078020902	45.407326595445404	19.976945719519975
20	14.267580604214267	24.58848795482459	42.006134085342005	19.13779735561914
21	13.1004576549131	26.45135615432645	41.63287430614163	18.815311884618815
22	11.39059851931139	24.4004857866244	39.119546050239116	25.089369643825087
23	11.844822735911844	24.882841714524883	36.03946673253604	27.232868817027235
24	13.55879870731356	23.936655619823938	36.76883280843677	25.735712864425736
25	12.58997001571259	27.110736021627112	33.38891061663339	26.91038334602691
26	12.160446813912161	28.506343357828506	35.56671675483557	23.766493073423767
27	15.473127354315471	25.85853179912586	37.35410963133736	21.314231215221312
28	13.549878896413551	22.67141475062267	38.10886286133811	25.66984349162567
29	15.773656367715775	21.592803771021593	36.504669177936506	26.128870683326127
30	24.156220195824158	23.845399092923845	32.51065231263251	19.48772839861949
31	22.854613943722853	29.885483350829883	27.626026635927627	19.633876069519633
32	19.655146387819656	28.454196771028457	28.439787845728443	23.450868995423452
33	24.91028728652491	21.813740625621815	27.746101013427747	25.529871074425532
34	23.226501444323226	22.58153050232258	28.458313606828455	25.733654446525733
35	20.647303815620646	26.489093815826486	28.784915913628783	24.078686454924078
36	28.071331041628074	27.315205533027314	26.316186712226315	18.297276713118297
37	29.597990984129595	23.822070356723824	28.998305235929	17.581633423217582
38	26.64004446182664	20.45998778672046	33.493203790233494	19.406763961219404
39	26.782075296926784	19.902842675119903	32.15797671243216	21.157105315521157
40	25.488702716425486	25.62249987992562	30.551038471830548	18.33775893181834
41	19.624270119319625	27.13063406132713	31.70992774953171	21.535168069821538
42	21.535854209121535	21.434991732021437	32.99712507633299	24.032028982524032
43	22.204153887322203	17.913038705117913	35.502905799935505	24.37990160762438
44	23.264239105823265	18.31099949911831	32.36656305963236	26.05819833542606
45	26.83765258022684	18.58751363701859	29.578092944429578	24.996740838324996
46	26.690132630726694	20.634953308220634	34.19032131903419	18.484592742018485
47	18.997138799119	24.308543120424307	37.20796196043721	19.486356120019487
48	17.69072957191769	21.732776188221735	38.613175246838615	21.963318993021964
49	23.82893174972383	16.17024488311617	37.65189408753765	22.348929279622347
50	22.681020700822682	14.713571149214713	33.98722408623399	28.61818406372862
51	18.87706442161888	14.937252561014938	31.246097582731245	34.93958543463494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	579.5
2	886.0
3	3500.5
4	6115.0
5	3913.5
6	1712.0
7	1644.5
8	1577.0
9	1522.5
10	1468.0
11	1370.5
12	1273.0
13	1235.5
14	1198.0
15	1119.5
16	1041.0
17	991.0
18	941.0
19	868.5
20	796.0
21	758.0
22	720.0
23	744.0
24	768.0
25	779.0
26	789.5
27	789.0
28	851.5
29	914.0
30	1047.5
31	1181.0
32	1226.5
33	1272.0
34	1587.5
35	1903.0
36	2000.0
37	2097.0
38	2342.0
39	2587.0
40	3145.0
41	3703.0
42	4799.5
43	5896.0
44	7166.0
45	8436.0
46	11665.5
47	14895.0
48	18661.5
49	22428.0
50	22779.0
51	23130.0
52	19127.5
53	15125.0
54	11138.0
55	7151.0
56	5360.5
57	3570.0
58	3032.5
59	2495.0
60	2299.0
61	2103.0
62	1954.0
63	1805.0
64	1650.0
65	1495.0
66	1263.5
67	1032.0
68	863.0
69	694.0
70	600.0
71	506.0
72	454.0
73	402.0
74	337.0
75	210.0
76	148.0
77	113.5
78	79.0
79	58.5
80	38.0
81	29.5
82	21.0
83	15.0
84	9.0
85	5.5
86	2.0
87	4.5
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	145743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.19069183425619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26329157203214	43.414778068243415
2	11.884875570859892	13.356387613813355
3	4.713410017827924	7.945493094007945
4	2.279776296187755	5.124088292405124
5	1.0684543434195424	3.001859437503002
6	0.6703787823283758	2.26014285420226
7	0.3834224729528415	1.5081341814015081
8	0.28329303734095296	1.2734745408012735
9	0.19781668986738957	1.0003910994010003
>10	1.1466041468239432	11.272582559711273
>50	0.07937089408259458	2.9901950694029904
>100	0.024421813563875256	2.6567313696026567
>500	0.003663272034581288	1.5513609573015512
>1k	0.0012210906781937626	2.6443808622026443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3854	2.6443808622026443	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	832	0.5708678976005709	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	768	0.526954982400527	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	661	0.4535380773004536	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	483	0.3314052819003314	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	457	0.31356566010031356	RNA PCR Primer, Index 33 (96% over 27bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	336	0.23054280480023054	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	275	0.1886883075001887	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.1578120390001578	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	193	0.13242488490013243	Illumina PCR Primer Index 5 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	180	0.1235050740001235	Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	172	0.11801595960011801	RNA PCR Primer, Index 33 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	172	0.11801595960011801	Illumina PCR Primer Index 5 (95% over 24bp)
GTGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	153	0.104979312900105	RNA PCR Primer, Index 33 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	151	0.10360703430010361	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5605758081005606	0.0
2	0.0	0.0	0.0	1.9054088361019055	0.0
3	0.0	0.0	0.0	2.6677095984026677	0.0
4	0.0	0.0	0.0	3.8732563485038733	0.0
5	0.0	0.0	0.0	6.551944175706552	0.0
6	0.0	0.0	0.0	8.985680272808986	0.0
7	0.0	0.0	0.0	11.063996212511064	0.0
8	0.0	0.0	0.0	14.022628874114023	0.0
9	0.0	0.0	0.0	15.552033373815553	0.0
10	0.0	0.0	0.0	18.795413844918794	0.0
11	0.0	0.0	0.0	26.699052441626698	0.0
12	0.0	0.0	0.0	33.855485340633855	0.0
13	0.0	0.0	0.0	36.0971024337361	0.0
14	0.0	0.0	0.0	36.842249713536845	0.0
15	0.0	0.0	0.0	38.02378158813802	0.0
16	0.0	0.0	0.0	40.75804669864076	0.0
17	0.0	0.0	0.0	43.923893428843925	0.0
18	0.0	0.0	0.0	46.66021695724666	0.0
19	0.0	0.0	0.0	48.25274627254825	0.0
20	0.0	0.0	0.0	49.54406043514955	0.0
21	0.0	0.0	0.0	51.11463329285112	0.0
22	0.0	0.0	0.0	52.435451445352435	0.0
23	0.0	0.0	0.0	53.527785210953525	0.0
24	0.0	0.0	0.0	54.07395209375407	0.0
25	0.0	0.0	0.0	54.414963325854416	0.0
26	6.861393000006862E-4	0.0	0.0	54.68667448865469	0.0
27	6.861393000006862E-4	0.0	0.0	54.913786596954914	0.0
28	6.861393000006862E-4	0.0	0.0	55.138154148055136	0.0
29	6.861393000006862E-4	0.0	0.0	55.31998106255532	0.0
30	6.861393000006862E-4	0.0	0.0	55.55121000665555	0.0
31	6.861393000006862E-4	0.0	0.0	55.73029236395573	0.0
32	6.861393000006862E-4	0.0	0.0	55.91898067145592	0.0
33	0.0013722786000013724	0.0	0.0	56.06650062095606	0.0
34	0.0013722786000013724	0.0	0.0	56.1996116451562	0.0
35	0.0013722786000013724	0.0	0.0	56.37457716665637	0.0
36	0.0013722786000013724	0.0	0.0	56.52621395195653	0.0
37	0.0013722786000013724	0.0	0.0	56.68196757305668	0.0
38	0.0013722786000013724	0.0	0.0	56.806844925656804	0.0
39	0.0013722786000013724	0.0	0.0	56.96534310395697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTGG	30	2.1524265E-6	45.000004	2
TTGTAGG	30	2.1524265E-6	45.000004	2
GAATATA	30	2.1524265E-6	45.000004	15
GTAGGGA	30	2.1524265E-6	45.000004	4
TGGCTTG	30	2.1524265E-6	45.000004	1
CATGGGC	40	6.7448127E-9	45.0	4
CTAGAAT	20	7.013E-4	45.0	41
CGGGTAT	20	7.013E-4	45.0	6
CTCACAA	20	7.013E-4	45.0	32
CTCGTCC	20	7.013E-4	45.0	37
CTACTCA	20	7.013E-4	45.0	29
GCGCGAC	25	3.874E-5	45.0	9
GGCACCG	20	7.013E-4	45.0	8
CAGCATA	20	7.013E-4	45.0	38
GGGTATT	25	3.874E-5	45.0	7
GGGTATG	55	1.8189894E-12	45.0	7
GTGGGAC	20	7.013E-4	45.0	5
TCTTAGG	20	7.013E-4	45.0	2
ATTAATC	20	7.013E-4	45.0	19
TTCGTTG	45	3.8016879E-10	45.0	1
>>END_MODULE