##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73196
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06277665446199	33.0	31.0	34.0	30.0	34.0
2	32.25107929395049	34.0	31.0	34.0	30.0	34.0
3	32.334840701677685	34.0	31.0	34.0	30.0	34.0
4	35.94399967211323	37.0	35.0	37.0	35.0	37.0
5	35.966910760150824	37.0	35.0	37.0	35.0	37.0
6	35.93923165200284	37.0	35.0	37.0	35.0	37.0
7	35.98162467894421	37.0	35.0	37.0	35.0	37.0
8	35.815017214055416	37.0	35.0	37.0	35.0	37.0
9	37.64871031203891	39.0	38.0	39.0	35.0	39.0
10	37.086671402808896	39.0	37.0	39.0	33.0	39.0
11	37.078078037051206	39.0	37.0	39.0	33.0	39.0
12	37.220203289797254	39.0	37.0	39.0	34.0	39.0
13	37.17554237936499	39.0	37.0	39.0	33.0	39.0
14	38.32164325919449	40.0	38.0	41.0	34.0	41.0
15	38.34531941636155	40.0	38.0	41.0	34.0	41.0
16	38.408915787747965	40.0	38.0	41.0	34.0	41.0
17	38.394475107929395	40.0	38.0	41.0	34.0	41.0
18	38.07363790371058	39.0	38.0	40.0	34.0	41.0
19	37.67720913711132	38.0	37.0	40.0	34.0	41.0
20	37.143300180337725	38.0	35.0	40.0	34.0	41.0
21	36.99598338707033	38.0	35.0	40.0	33.0	41.0
22	37.014618285152196	38.0	35.0	40.0	33.0	41.0
23	37.010997868736	38.0	35.0	40.0	33.0	41.0
24	36.932332367888954	38.0	35.0	40.0	33.0	41.0
25	36.81560467785125	38.0	35.0	40.0	33.0	41.0
26	36.69841248155637	38.0	35.0	40.0	33.0	41.0
27	36.60245095360402	38.0	35.0	40.0	33.0	41.0
28	36.5119815290453	38.0	35.0	40.0	32.0	41.0
29	36.475312858626154	38.0	35.0	40.0	32.0	41.0
30	36.2583611126291	38.0	35.0	40.0	31.0	41.0
31	35.78194163615498	38.0	35.0	40.0	30.0	41.0
32	35.20626810208208	38.0	35.0	40.0	25.0	41.0
33	34.61267009126182	38.0	34.0	40.0	21.0	41.0
34	34.06306355538554	38.0	34.0	40.0	18.0	41.0
35	33.579293950489095	38.0	33.0	40.0	15.0	41.0
36	33.20686922782666	38.0	33.0	40.0	12.0	41.0
37	32.96891906661566	37.0	32.0	40.0	10.0	41.0
38	32.96435597573638	38.0	32.0	40.0	10.0	41.0
39	33.048650199464454	38.0	33.0	40.0	10.0	41.0
40	32.883422591398435	38.0	32.0	40.0	10.0	41.0
41	32.76487786217826	38.0	31.0	40.0	10.0	41.0
42	32.58723154270725	37.0	31.0	40.0	10.0	41.0
43	32.25445379528936	37.0	31.0	40.0	10.0	41.0
44	31.998442537843598	37.0	30.0	40.0	9.0	41.0
45	32.10394010601672	37.0	31.0	40.0	10.0	41.0
46	31.914039018525603	37.0	30.0	40.0	8.0	41.0
47	31.72110497841412	37.0	29.0	40.0	8.0	41.0
48	31.73303185966446	37.0	29.0	40.0	8.0	41.0
49	31.769550248647466	37.0	29.0	40.0	8.0	41.0
50	31.703112191923054	36.0	29.0	40.0	8.0	41.0
51	30.758456746270287	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	8.0
17	20.0
18	27.0
19	61.0
20	113.0
21	148.0
22	259.0
23	465.0
24	835.0
25	1600.0
26	2435.0
27	2684.0
28	2260.0
29	2082.0
30	2021.0
31	2163.0
32	2357.0
33	2759.0
34	3896.0
35	5567.0
36	7069.0
37	8919.0
38	14144.0
39	11298.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.763484343406745	5.280343188152358	35.58664407891142	37.369528389529485
2	40.31094595333079	4.960653587627739	36.37357232635663	18.354828132684847
3	15.7863817694956	4.867752336193234	61.12629105415597	18.2195748401552
4	13.269850811519754	5.011202797967102	62.06759932236734	19.6513470681458
5	11.900923547734848	5.911525219957374	62.42690857423903	19.760642658068747
6	15.785015574621564	6.471665118312477	65.46942455871906	12.273894748346905
7	61.68369856276299	2.2774468550194	31.636974698070937	4.401879884146675
8	60.62899612000656	2.7378545275698123	30.918356194327558	5.714793158096071
9	55.94841248155637	5.13279414175638	33.02776107984043	5.891032296846823
10	24.825127056123286	21.480682004481118	39.73031313186513	13.963877807530467
11	16.52959178097164	17.662167331548172	45.06940269960107	20.738838187879118
12	15.798677523361931	14.565003552106672	50.461773867424455	19.174545057106947
13	18.752390841029566	16.973605115033607	50.285534728673696	13.98846931526313
14	16.35198644734685	18.307011312093557	49.24586042953167	16.095141811027926
15	12.086726050603858	17.126618940925734	51.73916607464889	19.047488933821523
16	11.536149516367015	16.899830591835617	49.468550193999675	22.095469697797693
17	11.029291218099349	17.290562325810154	47.83594731952566	23.84419913656484
18	12.454232471719767	16.003606754467455	51.42494125362042	20.11721952019236
19	12.99114705721624	18.160828460571615	51.98508115197552	16.862943330236625
20	13.753483796928794	21.109076998743102	48.98491720859063	16.152521995737473
21	12.458331056341878	22.633750478168206	49.173452101207715	15.734466364282202
22	10.531996283949944	21.326301983714956	47.3072299032734	20.834471829061698
23	10.89130553582163	21.50117492759167	45.104923766326024	22.50259577026067
24	12.23700748674791	20.827640854691513	45.214219356248975	21.721132302311602
25	11.066178479698344	23.315481720312587	42.5187168697743	23.099622930214768
26	10.480080878736544	25.525985026504184	44.1431225750041	19.850811519755176
27	11.637247937045741	24.505437455598667	46.16782337832668	17.68949122902891
28	9.508716323296355	21.755287174162522	47.232089185201374	21.503907317339745
29	12.347669271544893	20.54073993114378	45.46969779769386	21.641892999617465
30	17.562435105743482	22.72801792447675	42.54330837750697	17.1662385922728
31	15.909339308158915	29.02617629378654	37.31488059456801	17.74960380348653
32	15.290453030220231	27.364883326957756	36.66047324990437	20.684190392917646
33	19.460079785780643	23.255369145854964	35.58664407891142	21.697906989452974
34	18.242800153013825	22.529919667741407	35.02923657030439	24.198043608940377
35	17.36570304388218	27.192742772829114	32.877479643696375	22.564074539592326
36	18.72096835892672	33.641182578282965	30.70249740422974	16.935351658560577
37	16.910760150827915	33.54145035247827	32.60560686376305	16.94218263293076
38	19.152685939122357	27.422263511667307	35.96098147439751	17.464069074812834
39	24.713099076452266	20.421880977102575	35.74375648942566	19.12126345701951
40	24.724028635444558	20.476528772064047	32.691677140827366	22.107765451664026
41	23.42614350510957	23.908410295644572	29.35679545330346	23.3086507459424
42	27.151756926608012	24.501338870976554	30.698398819607633	17.6485053828078
43	22.979397781299525	23.087327176348435	36.2834034646702	17.649871577681843
44	19.62812175528717	21.991638887370897	35.6973058637084	22.68293349363353
45	23.256735340729	19.600797857806437	32.97447947975299	24.167987321711568
46	24.439860101644896	20.57352860812066	35.68774249959014	19.298868790644296
47	19.25924913929723	24.505437455598667	37.85725995956063	18.378053445543472
48	19.72512159134379	21.865948958959507	39.444778403191435	18.964151046505272
49	23.531340510410406	17.294660910432263	39.601890813705666	19.572107765451662
50	21.860484179463356	15.634734138477512	36.55117765998142	25.95360402207771
51	19.62812175528717	15.213946117274169	33.803759768293354	31.35417235914531
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	324.5
2	512.0
3	3311.5
4	6111.0
5	3818.0
6	1525.0
7	1433.5
8	1342.0
9	1303.5
10	1265.0
11	1226.5
12	1188.0
13	1153.5
14	1119.0
15	1016.0
16	913.0
17	851.5
18	790.0
19	729.0
20	668.0
21	626.0
22	584.0
23	566.5
24	549.0
25	508.0
26	493.5
27	520.0
28	538.5
29	557.0
30	631.5
31	706.0
32	809.0
33	912.0
34	1066.0
35	1220.0
36	1278.0
37	1336.0
38	1560.0
39	1784.0
40	2013.0
41	2242.0
42	2742.0
43	3242.0
44	3801.5
45	4361.0
46	5290.5
47	6220.0
48	7713.0
49	9206.0
50	9226.0
51	9246.0
52	7564.0
53	5882.0
54	4415.5
55	2949.0
56	2361.5
57	1774.0
58	1451.0
59	1128.0
60	1020.5
61	913.0
62	788.5
63	664.0
64	557.0
65	450.0
66	344.5
67	239.0
68	189.5
69	140.0
70	132.0
71	124.0
72	96.0
73	68.0
74	70.5
75	52.0
76	31.0
77	24.5
78	18.0
79	13.5
80	9.0
81	9.5
82	10.0
83	6.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	73196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.36958303732444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76060710589859	51.17765998141975
2	11.258192480165574	14.26853926444068
3	3.81812694032425	7.258593365757691
4	1.6320282856157295	4.136838078583529
5	0.7394791307347361	2.343024208973168
6	0.4225595032769921	1.6066451718673151
7	0.3104518799586064	1.3771244330291272
8	0.1832528458088996	0.9290125143450462
9	0.1358226974818903	0.7746324935788841
>10	0.6942048982407727	7.540029509809279
>50	0.028026905829596414	1.20771626864856
>100	0.015091410831321143	2.586206896551724
>500	0.0	0.0
>1k	0.002155915833045878	4.793977812995245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3509	4.793977812995245	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	435	0.5942947702060222	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	400	0.5464779496147331	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	330	0.4508443084321548	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	206	0.2814361440515875	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	201	0.27460516968140336	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	188	0.25684463631892457	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.18170391824689874	No Hit
TCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	88	0.12022514891524128	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79	0.10792939504890979	No Hit
GCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	76	0.10383081042679929	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	76	0.10383081042679929	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	75	0.10246461555276246	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5013935187715176	0.0
2	0.0	0.0	0.0	1.7337012951527406	0.0
3	0.0	0.0	0.0	2.4331930706595988	0.0
4	0.0	0.0	0.0	3.369036559374829	0.0
5	0.0	0.0	0.0	5.375976829334936	0.0
6	0.0	0.0	0.0	6.927974206240778	0.0
7	0.0	0.0	0.0	8.258648013552653	0.0
8	0.0	0.0	0.0	10.27378545275698	0.0
9	0.0	0.0	0.0	11.105798131045413	0.0
10	0.0	0.0	0.0	13.458385704136838	0.0
11	0.0	0.0	0.0	19.710093447729385	0.0
12	0.0	0.0	0.0	25.187168697743047	0.0
13	0.0	0.0	0.0	26.93179955188808	0.0
14	0.0	0.0	0.0	27.524728127220065	0.0
15	0.0	0.0	0.0	28.46330400568337	0.0
16	0.0	0.0	0.0	30.513962511612657	0.0
17	0.0	0.0	0.0	33.13295808514126	0.0
18	0.0	0.0	0.0	35.54429203781628	0.0
19	0.0	0.0	0.0	36.821684245040714	0.0
20	0.0	0.0	0.0	38.052625826547896	0.0
21	0.0	0.0	0.0	39.424285480080876	0.0
22	0.0	0.0	0.0	40.57462156401989	0.0
23	0.0	0.0	0.0	41.551450898956226	0.0
24	0.0	0.0	0.0	42.02278813049893	0.0
25	0.0	0.0	0.0	42.35613967976392	0.0
26	0.0	0.0	0.0	42.626646264823215	0.0
27	0.0	0.0	0.0	42.78102628558938	0.0
28	0.0	0.0	0.0	42.9859555166949	0.0
29	0.0	0.0	0.0	43.17858899393409	0.0
30	0.0	0.0	0.0	43.360292912180995	0.0
31	0.0	0.0	0.0	43.509208153451006	0.0
32	0.0	0.0	0.0	43.69227826657195	0.0
33	0.0	0.0	0.0	43.834362533471776	0.0
34	0.0	0.0	0.0	43.954587682387015	0.0
35	0.0	0.0	0.0	44.09940433903492	0.0
36	0.0	0.0	0.0	44.24012241106071	0.0
37	0.0	0.0	0.0	44.37127711896825	0.0
38	0.0	0.0	0.0	44.52565713973441	0.0
39	0.0	0.0	0.0	44.68960052461883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	25	3.854977E-5	45.000004	8
TCCGGGA	35	1.1912016E-7	45.000004	4
GACCGAT	25	3.854977E-5	45.000004	9
ATTTGGG	25	3.854977E-5	45.000004	3
TGGGCAT	25	3.854977E-5	45.000004	6
CTATGGG	35	1.1912016E-7	45.000004	3
TTTGCGG	25	3.854977E-5	45.000004	2
ATGGGCT	20	6.9900084E-4	45.0	5
GCCTTGG	20	6.9900084E-4	45.0	2
GATACTG	20	6.9900084E-4	45.0	1
GCGATTT	20	6.9900084E-4	45.0	9
TCAGGGC	20	6.9900084E-4	45.0	4
GAAGCTA	20	6.9900084E-4	45.0	7
AGCTACG	20	6.9900084E-4	45.0	9
TCAACAT	20	6.9900084E-4	45.0	28
ACATCAA	20	6.9900084E-4	45.0	25
GCTACGA	20	6.9900084E-4	45.0	10
TTGGGTC	55	1.8189894E-12	45.0	5
GTGGGAC	20	6.9900084E-4	45.0	5
CGAATAT	20	6.9900084E-4	45.0	14
>>END_MODULE