##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73170
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.978720787207873	33.0	31.0	34.0	30.0	34.0
2	32.212204455377886	34.0	31.0	34.0	30.0	34.0
3	32.29557195571956	34.0	31.0	34.0	30.0	34.0
4	35.86744567445675	37.0	35.0	37.0	35.0	37.0
5	35.95865791991253	37.0	35.0	37.0	35.0	37.0
6	35.96508131747984	37.0	35.0	37.0	35.0	37.0
7	35.92838595052617	37.0	35.0	37.0	35.0	37.0
8	35.76678966789668	37.0	35.0	37.0	35.0	37.0
9	37.612491458247916	39.0	37.0	39.0	35.0	39.0
10	37.107899412327455	39.0	37.0	39.0	33.0	39.0
11	37.10240535738691	39.0	37.0	39.0	33.0	39.0
12	37.21544348776821	39.0	37.0	39.0	34.0	39.0
13	37.21407680743474	39.0	37.0	39.0	34.0	39.0
14	38.35612956129561	40.0	38.0	41.0	34.0	41.0
15	38.37248872488725	40.0	38.0	41.0	34.0	41.0
16	38.49845565122318	40.0	38.0	41.0	34.0	41.0
17	38.55584255842558	40.0	38.0	41.0	34.0	41.0
18	38.25146918135848	39.0	38.0	40.0	34.0	41.0
19	37.89793631269646	39.0	37.0	40.0	34.0	41.0
20	37.32354790214569	39.0	35.0	40.0	34.0	41.0
21	37.218996856635236	38.0	35.0	40.0	33.0	41.0
22	37.23389367227006	38.0	35.0	40.0	33.0	41.0
23	37.15263085964193	38.0	35.0	40.0	33.0	41.0
24	37.06702200355337	38.0	35.0	40.0	33.0	41.0
25	37.002405357386905	38.0	35.0	40.0	33.0	41.0
26	36.95429820964876	38.0	35.0	40.0	33.0	41.0
27	36.87243405767391	38.0	35.0	40.0	33.0	41.0
28	36.84844881782151	38.0	35.0	40.0	33.0	41.0
29	36.66542298756321	38.0	35.0	40.0	33.0	41.0
30	36.39225092250923	38.0	35.0	40.0	32.0	41.0
31	35.95317753177532	38.0	35.0	40.0	30.0	41.0
32	35.45190651906519	38.0	35.0	40.0	27.0	41.0
33	34.756771901052346	38.0	35.0	40.0	21.0	41.0
34	34.1829848298483	38.0	34.0	40.0	17.0	41.0
35	33.785731857318574	38.0	34.0	40.0	15.0	41.0
36	33.41526581932486	38.0	33.0	40.0	12.0	41.0
37	33.21681016810168	38.0	33.0	40.0	10.0	41.0
38	33.182807161404945	38.0	33.0	40.0	10.0	41.0
39	33.277217438841056	38.0	33.0	40.0	10.0	41.0
40	33.10333470001367	38.0	33.0	40.0	10.0	41.0
41	33.013393467268	38.0	32.0	40.0	10.0	41.0
42	32.772529725297254	38.0	31.0	40.0	10.0	41.0
43	32.479663796637965	37.0	31.0	40.0	10.0	41.0
44	32.25215252152522	37.0	31.0	40.0	10.0	41.0
45	32.27673910072434	37.0	31.0	40.0	10.0	41.0
46	32.04540112067787	37.0	30.0	40.0	8.0	41.0
47	31.98726253929206	37.0	30.0	40.0	8.0	41.0
48	31.90997676643433	37.0	30.0	40.0	8.0	41.0
49	31.92722427224272	37.0	30.0	40.0	8.0	41.0
50	31.76493098264316	37.0	29.0	40.0	8.0	41.0
51	30.93329233292333	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	2.0
13	4.0
14	3.0
15	6.0
16	7.0
17	7.0
18	33.0
19	52.0
20	71.0
21	170.0
22	282.0
23	430.0
24	881.0
25	1578.0
26	2421.0
27	2586.0
28	2298.0
29	2000.0
30	1949.0
31	2064.0
32	2158.0
33	2688.0
34	3818.0
35	5261.0
36	6519.0
37	8118.0
38	14319.0
39	13436.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.084187508541753	5.709990433237666	34.66311329779965	37.542708760420936
2	40.448271149378165	4.992483258165914	36.18149514828482	18.37775044417111
3	15.998359983599835	4.941916085827525	60.97990979909799	18.07981413147465
4	13.795271286046193	5.056717233839005	61.59628262949296	19.55172885062184
5	11.653683203498701	5.946426130927976	62.37529041957086	20.02460024600246
6	15.697690310236437	6.655733223998907	65.63892305589722	12.007653409867432
7	61.354380210468776	2.3821238212382125	31.866885335520024	4.396610632772995
8	60.2323356566899	2.7634276342763426	31.059177258439252	5.945059450594506
9	55.592455924559246	5.070384037173705	33.24996583299166	6.087194205275386
10	24.451277846111793	21.216345496788303	40.26923602569359	14.063140631406315
11	16.70903375700424	17.541342080087468	45.03621702883696	20.71340713407134
12	15.848025146918134	14.455377887112206	50.63140631406314	19.06519065190652
13	18.724887248872488	17.157304906382397	50.21183545168785	13.905972393057265
14	16.166461664616648	18.359983599836	49.42462757960913	16.048927155938227
15	12.171655049883832	17.285772857728578	51.85731857318573	18.685253519201858
16	11.70288369550362	16.818368183681837	49.349460161268276	22.12928795954626
17	10.964876315429821	17.116304496378298	48.077080770807704	23.84173841738417
18	12.60079267459341	16.241629082957495	51.260079267459346	19.897498974989748
19	13.222632226322265	18.178215115484488	51.65641656416564	16.94273609402761
20	13.691403580702474	20.96214295476288	49.11985786524532	16.226595599289325
21	12.566625666256662	22.555692223588903	49.05425720923876	15.823424900915676
22	10.69427360940276	21.0796774634413	47.28850621839552	20.93754270876042
23	10.948476151428181	21.456881235479024	44.96378297116305	22.630859641929753
24	12.323356566899001	20.77764110974443	45.427087604209376	21.471914719147193
25	10.902009020090201	23.485034850348505	42.78256115894492	22.83039497061637
26	10.515238485718191	25.250785841191746	44.269509361760285	19.96446631132978
27	11.676916769167692	24.351510181768486	46.219762197621975	17.75181085144185
28	9.4943282766161	22.081454147874812	47.083504168375015	21.34071340713407
29	12.103321033210332	20.803608036080362	45.82205822058221	21.271012710127103
30	17.492141588082546	22.931529315293155	42.349323493234934	17.227005603389365
31	16.1049610496105	28.832854995216618	37.23110564438978	17.83107831078311
32	15.410687440207735	27.441574415744157	36.78283449501162	20.36490364903649
33	19.40002733360667	23.392100587672545	35.15238485718191	22.055487221538883
34	17.914445811124775	22.923329233292332	35.082684160174935	24.079540795407954
35	17.477108104414377	27.188738554052204	33.002596692633595	22.331556648899824
36	18.902555692223586	33.76793767937679	30.72024053573869	16.609266092660928
37	16.703567035670357	33.38390050567172	33.009430094300946	16.903102364356975
38	19.37406040727074	27.624709580429137	35.48858821921553	17.512641793084597
39	24.851715183818506	20.38677053437201	35.737324039907065	19.02419024190242
40	24.817548175481754	20.55350553505535	32.54339210058767	22.08555418887522
41	23.45360120267869	23.998906655733222	29.60092934262676	22.946562798961324
42	26.96460297936313	24.771081044143774	30.38950389503895	17.87481208145415
43	23.214432144321442	23.334700013666804	36.06532731993987	17.38554052207189
44	19.781331146644803	22.18395517288506	35.694956949569494	22.339756730900643
45	23.079130791307914	20.168101681016807	32.82629492961596	23.926472598059316
46	24.531911985786525	20.84460844608446	35.433921005876726	19.18955856225229
47	19.559928932622658	24.493644936449364	37.85704523711904	18.089380893808936
48	19.74169741697417	22.141588082547493	39.22645893125598	18.890255569222358
49	23.418067514008474	17.479841465081318	39.80456471231379	19.297526308596417
50	22.148421484214843	15.794724613912805	36.565532321989885	25.491321579882463
51	19.73349733497335	15.297252972529726	33.97020636873035	30.99904332376657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	329.5
2	503.0
3	3305.5
4	6108.0
5	3862.0
6	1616.0
7	1479.5
8	1343.0
9	1318.0
10	1293.0
11	1218.5
12	1144.0
13	1102.0
14	1060.0
15	993.5
16	927.0
17	854.5
18	782.0
19	751.5
20	721.0
21	672.5
22	624.0
23	554.5
24	485.0
25	494.0
26	501.0
27	499.0
28	523.0
29	547.0
30	610.0
31	673.0
32	801.0
33	929.0
34	1057.5
35	1186.0
36	1272.0
37	1358.0
38	1582.0
39	1806.0
40	2029.5
41	2253.0
42	2782.5
43	3312.0
44	3842.5
45	4373.0
46	5303.0
47	6233.0
48	7745.5
49	9258.0
50	9118.0
51	8978.0
52	7402.0
53	5826.0
54	4448.5
55	3071.0
56	2406.5
57	1742.0
58	1401.5
59	1061.0
60	980.5
61	900.0
62	822.0
63	744.0
64	597.0
65	450.0
66	357.0
67	264.0
68	205.0
69	146.0
70	119.5
71	93.0
72	80.0
73	67.0
74	64.0
75	48.0
76	35.0
77	27.5
78	20.0
79	16.0
80	12.0
81	9.5
82	7.0
83	4.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	73170.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.722837228372285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14571269248917	51.708350416837504
2	10.805130184875392	13.770671040043734
3	3.9291382490455966	7.511275112751127
4	1.5034530090507443	3.832171655049884
5	0.8450220906790203	2.6923602569359026
6	0.44824775876120615	1.7138171381713818
7	0.28310384763865654	1.2628126281262813
8	0.2058937073735684	1.049610496104961
9	0.13940719770085358	0.7995079950799507
>10	0.6477072877793506	7.083504168375017
>50	0.03002616565864539	1.2778461117944513
>100	0.015013082829322694	2.5679923465901324
>500	0.0	0.0
>1k	0.002144726118474671	4.730080634139674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3461	4.730080634139674	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	436	0.5958726253929206	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	417	0.5699056990569906	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	293	0.4004373377067104	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	228	0.3116031160311603	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	188	0.2569359026923602	No Hit
TCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	187	0.25556922235889024	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.17766844335110019	No Hit
GGCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	85	0.11616782834495012	No Hit
GCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	78	0.10660106601066012	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.10523438567719011	No Hit
TCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	76	0.10386770534372011	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	75	0.1025010250102501	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	74	0.10113434467678009	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013666803334700013	0.0	0.0	0.4359710263769304	0.0
2	0.0013666803334700013	0.0	0.0	1.6741834085007516	0.0
3	0.0013666803334700013	0.0	0.0	2.3301899685663523	0.0
4	0.0013666803334700013	0.0	0.0	3.3032663659969934	0.0
5	0.0013666803334700013	0.0	0.0	5.308186415197485	0.0
6	0.0013666803334700013	0.0	0.0	6.763700970343037	0.0
7	0.0013666803334700013	0.0	0.0	8.064780647806478	0.0
8	0.0013666803334700013	0.0	0.0	10.06696733634003	0.0
9	0.0013666803334700013	0.0	0.0	11.020910209102091	0.0
10	0.0013666803334700013	0.0	0.0	13.366133661336614	0.0
11	0.0013666803334700013	0.0	0.0	19.40686073527402	0.0
12	0.0013666803334700013	0.0	0.0	24.779281126144596	0.0
13	0.0013666803334700013	0.0	0.0	26.520431870985377	0.0
14	0.0013666803334700013	0.0	0.0	27.076670766707668	0.0
15	0.0013666803334700013	0.0	0.0	27.883012163454968	0.0
16	0.0013666803334700013	0.0	0.0	30.00956676233429	0.0
17	0.0013666803334700013	0.0	0.0	32.614459477928115	0.0
18	0.0013666803334700013	0.0	0.0	34.99111657783244	0.0
19	0.0013666803334700013	0.0	0.0	36.319529861965286	0.0
20	0.0013666803334700013	0.0	0.0	37.48120814541479	0.0
21	0.0013666803334700013	0.0	0.0	38.8519885198852	0.0
22	0.0013666803334700013	0.0	0.0	40.06013393467268	0.0
23	0.0013666803334700013	0.0	0.0	40.97580975809758	0.0
24	0.0013666803334700013	0.0	0.0	41.41724750580839	0.0
25	0.0013666803334700013	0.0	0.0	41.723383900505674	0.0
26	0.0013666803334700013	0.0	0.0	41.93385267186005	0.0
27	0.0013666803334700013	0.0	0.0	42.103321033210335	0.0
28	0.0013666803334700013	0.0	0.0	42.32198988656553	0.0
29	0.0013666803334700013	0.0	0.0	42.47642476424764	0.0
30	0.0013666803334700013	0.0	0.0	42.66912669126691	0.0
31	0.0013666803334700013	0.0	0.0	42.831761650949844	0.0
32	0.0013666803334700013	0.0	0.0	42.97116304496378	0.0
33	0.0013666803334700013	0.0	0.0	43.10919775864425	0.0
34	0.0013666803334700013	0.0	0.0	43.2663659969933	0.0
35	0.0013666803334700013	0.0	0.0	43.390733907339076	0.0
36	0.0013666803334700013	0.0	0.0	43.53286866201995	0.0
37	0.0013666803334700013	0.0	0.0	43.68320349870165	0.0
38	0.0013666803334700013	0.0	0.0	43.84857181905152	0.0
39	0.0013666803334700013	0.0	0.0	44.00027333606669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGTTG	25	3.854977E-5	45.000004	1
ACTGAAG	25	3.854977E-5	45.000004	4
GTTCTTG	25	3.854977E-5	45.000004	1
TGCTCTG	35	1.1912016E-7	45.000004	1
CGAATAT	25	3.854977E-5	45.000004	14
TACTGAA	25	3.854977E-5	45.000004	3
AAGCTAC	25	3.854977E-5	45.000004	8
TTCGGGA	50	2.1827873E-11	45.000004	4
GCCCTAA	20	6.9900084E-4	45.0	40
GCGGGAT	20	6.9900084E-4	45.0	5
TTCCGTG	20	6.9900084E-4	45.0	1
ACTTGGG	20	6.9900084E-4	45.0	3
GCGATGG	20	6.9900084E-4	45.0	9
CCAAAGT	20	6.9900084E-4	45.0	32
GTACTGG	20	6.9900084E-4	45.0	2
CCTAACT	20	6.9900084E-4	45.0	42
CAACTAG	20	6.9900084E-4	45.0	16
GGCGATC	20	6.9900084E-4	45.0	8
GACATTA	20	6.9900084E-4	45.0	20
ACATTAA	20	6.9900084E-4	45.0	21
>>END_MODULE