FastQCFastQC Report
Sat 14 Jan 2017
SRR2933602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256410
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600894.782594853590787No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC20690.16467554381133548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT207.033539E-445.032
CGATACG207.033539E-445.010
TACCGGT207.033539E-445.040
CGTTTTT293600.044.3179471
GTTTTTT331100.040.086832
GTTTGCG2200.039.8863641
CTATCGG403.458572E-739.3752
TGGGCGA20750.038.7108426
ACGTCTT301.1399825E-437.49999628
GTAGGGC1150.037.1739164
TTTGGGC33800.036.9452674
CGTTTGG5500.036.8181842
TTGGGCA14050.036.6725965
GGGCGAT30250.036.446287
CGGGATA2100.036.428576
GATACGT250.002107183435.99999610
CCGATAA504.879621E-835.99999615
TATTGCG504.879621E-835.9999961
AACGCGC250.002107183435.99999621
CCGGTAT250.002107183435.99999642
Produced by FastQC (version 0.11.5)