FastQCFastQC Report
Sat 14 Jan 2017
SRR2933601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268952
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599814.7268139378006415No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC20100.15839842641802054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG207.0335635E-445.0000041
ACGTATA207.0335635E-445.00000412
CGTTTTT290600.044.0708851
CGAATAG850.042.3529426
GTTTTTT326650.040.0061232
GCTCGTA900.040.032
GCGATAT356.248805E-638.5714269
TACGGGA1350.038.3333364
TCGTAAC950.037.89473734
TTGGGAC15750.037.1428535
TGGGCGA19900.037.0854266
TCTGGGA20800.036.129814
TTTGGGC33000.035.7272724
GGTTAAC702.1827873E-1135.3571438
GGGCGAT28850.035.2512977
TGGGACT12900.035.232566
GTTTGCG1600.035.1562541
TGGGAAC12550.035.1394426
TATTGCG458.700881E-735.01
TTGGGTA9000.035.05
Produced by FastQC (version 0.11.5)