##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1268952
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32803053228176	33.0	31.0	34.0	31.0	34.0
2	32.532686027525074	34.0	31.0	34.0	31.0	34.0
3	32.59619039963687	34.0	31.0	34.0	31.0	34.0
4	36.07870983299605	37.0	35.0	37.0	35.0	37.0
5	36.12587237342311	37.0	35.0	37.0	35.0	37.0
6	36.114127248311995	37.0	36.0	37.0	35.0	37.0
7	36.11892175590566	37.0	36.0	37.0	35.0	37.0
8	36.03176321878211	37.0	37.0	37.0	35.0	37.0
9	37.88275758263512	39.0	38.0	39.0	35.0	39.0
10	37.40338405235186	39.0	37.0	39.0	34.0	39.0
11	37.32140143992838	39.0	37.0	39.0	34.0	39.0
12	37.20357507612581	39.0	37.0	39.0	34.0	39.0
13	37.17564178944515	39.0	37.0	39.0	34.0	39.0
14	38.40361179934308	40.0	38.0	41.0	34.0	41.0
15	38.47356795213688	40.0	38.0	41.0	34.0	41.0
16	38.57238098840618	40.0	38.0	41.0	34.0	41.0
17	38.51954053423613	40.0	38.0	41.0	34.0	41.0
18	38.180493824825525	39.0	38.0	41.0	34.0	41.0
19	37.77115446447147	39.0	37.0	41.0	34.0	41.0
20	37.23479375106387	38.0	35.0	41.0	34.0	41.0
21	37.165858913497125	38.0	35.0	40.0	33.0	41.0
22	37.14597478864449	38.0	35.0	40.0	33.0	41.0
23	37.00654555885487	38.0	35.0	40.0	33.0	41.0
24	36.90373473543522	38.0	35.0	40.0	33.0	41.0
25	36.84206967639438	38.0	35.0	40.0	33.0	41.0
26	36.818069556610496	38.0	35.0	40.0	33.0	41.0
27	36.75267937636727	38.0	35.0	40.0	33.0	41.0
28	36.65599408015433	38.0	35.0	40.0	33.0	41.0
29	36.464673210649416	38.0	35.0	40.0	33.0	41.0
30	36.17394747791879	38.0	35.0	40.0	31.0	41.0
31	35.799550337601424	38.0	35.0	40.0	30.0	41.0
32	35.30365924006582	38.0	35.0	40.0	27.0	41.0
33	34.53637962665255	38.0	34.0	40.0	21.0	41.0
34	33.889329935253656	38.0	34.0	40.0	16.0	41.0
35	33.45193750433428	37.0	33.0	40.0	15.0	41.0
36	33.172692899337406	37.0	33.0	40.0	10.0	41.0
37	33.02520032278605	37.0	33.0	40.0	10.0	41.0
38	32.867234536846155	37.0	33.0	40.0	10.0	41.0
39	32.80856643907729	37.0	33.0	40.0	10.0	41.0
40	32.62804503243621	37.0	32.0	40.0	10.0	41.0
41	32.45699522125344	36.0	32.0	40.0	10.0	41.0
42	32.24994089610954	36.0	31.0	40.0	10.0	41.0
43	32.130764599448995	36.0	31.0	40.0	9.0	41.0
44	31.959541416854222	36.0	31.0	40.0	8.0	41.0
45	31.85120083344366	35.0	31.0	40.0	8.0	41.0
46	31.695379336649456	35.0	30.0	40.0	8.0	41.0
47	31.65490105220686	35.0	30.0	40.0	8.0	41.0
48	31.545046621148792	35.0	30.0	40.0	8.0	41.0
49	31.52186528726067	35.0	30.0	40.0	8.0	41.0
50	31.35083123711535	35.0	29.0	40.0	8.0	41.0
51	30.57559151173567	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	11.0
11	10.0
12	16.0
13	25.0
14	29.0
15	67.0
16	123.0
17	251.0
18	503.0
19	910.0
20	1766.0
21	2758.0
22	4199.0
23	7144.0
24	13359.0
25	26603.0
26	43857.0
27	50000.0
28	45107.0
29	38171.0
30	34252.0
31	34836.0
32	38803.0
33	48962.0
34	78184.0
35	90227.0
36	110571.0
37	134368.0
38	221128.0
39	242605.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.655309263076933	3.912756353274198	38.69184965231152	34.740084731337355
2	40.66465871049496	4.283534759392002	39.81009525971038	15.241711270402664
3	18.037482899274362	4.609788234700761	61.461899268057415	15.890829597967457
4	14.80071744242493	4.516010061846311	61.95648062338055	18.726791872348205
5	14.0816989137493	5.321635491334582	61.241875185192185	19.35479040972393
6	17.203645212742483	6.2535856360209054	63.04572592186308	13.497043229373531
7	65.03650256274469	1.9785618368543492	29.407495318971876	3.5774402814290847
8	66.11810375806178	2.4076560815539123	28.082543705356862	3.3916964550274558
9	61.39318114475567	4.141606617113966	29.811844734867826	4.653367503262535
10	31.60143173264237	18.2387513475687	36.062593384146915	14.097223535642009
11	24.268057420611655	19.024596675051537	40.91904185501106	15.788304049325744
12	19.87986937252158	18.27232235734685	44.937397159230606	16.910411110900963
13	19.085513084813293	17.564021334140296	47.21234530541739	16.138120275629024
14	17.056437122917178	18.023849601876194	48.282834969329016	16.636878305877605
15	16.559964443099503	17.416498023565904	48.758109053770355	17.26542847956424
16	18.61772549316286	17.440139579747697	46.68450816106519	17.25762676602425
17	18.270431032852308	18.490927946841175	45.781243104546114	17.457397915760406
18	19.504441460354684	17.210422458847933	45.73246269362434	17.552673387173037
19	18.7855805420536	20.482729055157325	43.507004205044794	17.22468619774428
20	20.024713306728703	21.40065187650912	42.339741771162345	16.234893045599833
21	19.585295582496425	22.37027090071177	42.37914436479867	15.665289151993141
22	18.858948171404435	19.47882977449108	43.33804588353224	18.32417617057225
23	17.63526122343477	21.412787875349107	42.62320402978206	18.328746871434063
24	17.388758597645932	20.983930046211363	43.4012476437249	18.226063712417805
25	18.16664460121423	21.956622472717644	41.35499215100335	18.521740775064778
26	17.564572970451206	20.745071523587967	42.65945441592747	19.03090109003335
27	17.677973634936546	19.831877013472536	43.223935972361446	19.266213379229473
28	16.659889420561218	21.379453281132776	42.49443635377855	19.46622094452745
29	18.067586480812516	20.53403123207182	42.131538466388015	19.266843820727654
30	19.376855862160273	20.196272199421255	42.72935461703831	17.69751732138016
31	19.217590578682252	21.91848076207768	40.253138022557195	18.610790636682868
32	19.045874075615153	22.011943714183044	39.216613394360074	19.725568815841736
33	19.185674477836827	23.042163927398356	36.677195039686296	21.09496655507852
34	17.744327602620114	23.889871326890223	37.02157370806776	21.344227362421904
35	17.492702639658553	26.341027871818635	35.38754814996942	20.77872133855339
36	18.233392594834164	27.995148752671493	32.41210069411609	21.359357958378254
37	17.970262074530794	27.67291434191364	31.742571823047683	22.614251760507884
38	18.234889893392342	27.835804664006204	31.974101463254716	21.955203979346734
39	18.76477597261362	26.1855452373297	31.31946677258084	23.73021201747584
40	20.19548414754853	25.71492065893745	32.09703755540005	21.992557638113972
41	18.23544152970325	26.58926421172747	31.844466930191214	23.330827328378064
42	19.149818117627778	26.781942894609095	32.52250676148507	21.545732226278062
43	19.15241868880777	25.198116240803436	33.931307094358175	21.718157976030614
44	19.96860401339058	25.46778759165043	33.152239012980786	21.411369381978197
45	19.755751202567158	24.970132834023666	32.78681935959752	22.48729660381165
46	19.668671470630883	25.693249232437477	32.927880644815566	21.710198652116077
47	18.339385571715873	27.088416267912418	33.84958611515644	20.722612045215264
48	18.507083010232066	26.515502556440275	33.879453281132776	21.097961152194884
49	19.60428763262913	25.272350727214267	33.53799040468039	21.585371235476202
50	18.36428801089403	25.105520145758074	33.75817209792017	22.772019745427723
51	18.14552481102516	24.699437015742124	33.25610424980614	23.898933923426576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	873.0
1	2266.0
2	3659.0
3	49400.5
4	95142.0
5	61211.0
6	27280.0
7	25960.0
8	24640.0
9	24240.0
10	23840.0
11	23221.5
12	22603.0
13	21787.5
14	20972.0
15	19747.5
16	18523.0
17	17816.0
18	17109.0
19	16234.0
20	15359.0
21	14761.5
22	14164.0
23	13688.5
24	13213.0
25	13523.5
26	14136.0
27	14438.0
28	14898.5
29	15359.0
30	16553.5
31	17748.0
32	19869.5
33	21991.0
34	24187.5
35	26384.0
36	27846.0
37	29308.0
38	30954.5
39	32601.0
40	34932.0
41	37263.0
42	41332.5
43	45402.0
44	50460.5
45	55519.0
46	66004.0
47	76489.0
48	83798.0
49	91107.0
50	92691.0
51	94275.0
52	86774.0
53	79273.0
54	70744.5
55	62216.0
56	56638.0
57	51060.0
58	47923.5
59	44787.0
60	41755.5
61	38724.0
62	35645.5
63	32567.0
64	29368.0
65	26169.0
66	23182.0
67	20195.0
68	17204.5
69	14214.0
70	11853.5
71	9493.0
72	8333.5
73	7174.0
74	6663.5
75	5020.5
76	3888.0
77	2876.0
78	1864.0
79	1543.5
80	1223.0
81	828.5
82	434.0
83	369.5
84	305.0
85	186.5
86	68.0
87	45.0
88	22.0
89	21.5
90	21.0
91	13.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1268952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.63789771812457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40067959811266	30.28362323505196
2	8.597389162364511	6.815648645214342
3	3.5692911223784747	4.244375893051441
4	2.1259030515313455	3.3706531086099347
5	1.3854450482676488	2.745806455865761
6	1.0684684234718422	2.5411105250773613
7	0.8660471368311675	2.402980148015192
8	0.7101427731245143	2.2518853285099873
9	0.6306881263232619	2.249923629891337
>10	4.487912700421619	30.199330509131705
>50	0.09459252124538231	2.573094817068302
>100	0.059224364976953145	4.202793362924684
>500	0.003412928864788712	0.9988536439324909
>1k	6.022815643744785E-4	0.3467976561709525
>5k	0.0	0.0
>10k+	2.0076052145815955E-4	4.773123041484602
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59981	4.7268139378006415	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	2010	0.15839842641802054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.728311236358822E-4	0.0	0.0	0.07013661667265586	0.0
2	4.728311236358822E-4	0.0	0.0	0.23200247133067287	0.0
3	4.728311236358822E-4	0.0	0.0	0.40758042857413046	0.0
4	4.728311236358822E-4	0.0	0.0	0.6545558854866063	0.0
5	5.516363109085293E-4	0.0	0.0	1.2827120332368758	0.0
6	5.516363109085293E-4	0.0	0.0	2.3084403507776496	0.0
7	5.516363109085293E-4	0.0	0.0	3.2290425484967122	0.0
8	5.516363109085293E-4	0.0	0.0	4.566366576513532	0.0
9	6.304414981811762E-4	0.0	0.0	5.608407567819744	0.0
10	6.304414981811762E-4	0.0	0.0	6.960862191792913	0.0
11	6.304414981811762E-4	0.0	0.0	8.57290110264218	0.0
12	6.304414981811762E-4	0.0	0.0	9.84473802003543	0.0
13	6.304414981811762E-4	0.0	0.0	10.432230691153014	0.0
14	6.304414981811762E-4	0.0	0.0	10.776136528410847	0.0
15	6.304414981811762E-4	0.0	0.0	11.0456502688833	0.0
16	6.304414981811762E-4	0.0	0.0	11.424466804102913	0.0
17	6.304414981811762E-4	0.0	0.0	11.887210863767896	0.0
18	6.304414981811762E-4	0.0	0.0	12.354604429481967	0.0
19	6.304414981811762E-4	0.0	0.0	12.680306268479816	0.0
20	6.304414981811762E-4	0.0	0.0	12.98827694034132	0.0
21	6.304414981811762E-4	0.0	0.0	13.320283194320982	0.0
22	6.304414981811762E-4	0.0	0.0	13.673645654051532	0.0
23	6.304414981811762E-4	0.0	0.0	14.002893726476652	0.0
24	6.304414981811762E-4	0.0	0.0	14.269570480207289	0.0
25	6.304414981811762E-4	0.0	0.0	14.495189731368878	0.0
26	6.304414981811762E-4	0.0	0.0	14.713322489739564	0.0
27	6.304414981811762E-4	0.0	0.0	14.950841324179322	0.0
28	7.092466854538233E-4	0.0	0.0	15.183789457757268	0.0
29	7.092466854538233E-4	0.0	0.0	15.430449693920652	0.0
30	7.092466854538233E-4	0.0	0.0	15.722659328327628	0.0
31	7.880518727264703E-4	0.0	0.0	15.940161645200133	0.0
32	7.880518727264703E-4	0.0	0.0	16.239069720525283	0.0
33	7.880518727264703E-4	0.0	0.0	16.487306060434122	0.0
34	8.668570599991174E-4	0.0	0.0	16.73767014039932	0.0
35	8.668570599991174E-4	0.0	0.0	17.016955724093584	0.0
36	8.668570599991174E-4	0.0	0.0	17.258257207522426	0.0
37	9.456622472717644E-4	0.0	0.0	17.50988217048399	0.0
38	9.456622472717644E-4	0.0	0.0	17.834559542047295	0.0
39	9.456622472717644E-4	0.0	0.0	18.316610872594076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATCG	20	7.0335635E-4	45.000004	1
ACGTATA	20	7.0335635E-4	45.000004	12
CGTTTTT	29060	0.0	44.070885	1
CGAATAG	85	0.0	42.35294	26
GTTTTTT	32665	0.0	40.006123	2
GCTCGTA	90	0.0	40.0	32
GCGATAT	35	6.248805E-6	38.571426	9
TACGGGA	135	0.0	38.333336	4
TCGTAAC	95	0.0	37.894737	34
TTGGGAC	1575	0.0	37.142853	5
TGGGCGA	1990	0.0	37.085426	6
TCTGGGA	2080	0.0	36.12981	4
TTTGGGC	3300	0.0	35.727272	4
GGTTAAC	70	2.1827873E-11	35.357143	8
GGGCGAT	2885	0.0	35.251297	7
TGGGACT	1290	0.0	35.23256	6
GTTTGCG	160	0.0	35.156254	1
TGGGAAC	1255	0.0	35.139442	6
TATTGCG	45	8.700881E-7	35.0	1
TTGGGTA	900	0.0	35.0	5
>>END_MODULE