##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392098
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.239355977332195	33.0	31.0	34.0	30.0	34.0
2	32.40404184668119	34.0	31.0	34.0	30.0	34.0
3	32.52845972180424	34.0	31.0	34.0	31.0	34.0
4	36.02915597631205	37.0	35.0	37.0	35.0	37.0
5	36.02118858040592	37.0	35.0	37.0	35.0	37.0
6	35.97999224683625	37.0	35.0	37.0	35.0	37.0
7	36.02586598248397	37.0	35.0	37.0	35.0	37.0
8	35.887275630072075	37.0	35.0	37.0	35.0	37.0
9	37.72470912884024	39.0	38.0	39.0	35.0	39.0
10	37.22735387581676	39.0	37.0	39.0	34.0	39.0
11	37.14919994491173	39.0	37.0	39.0	34.0	39.0
12	36.97526638748476	39.0	37.0	39.0	33.0	39.0
13	36.883416900876824	39.0	37.0	39.0	33.0	39.0
14	37.859338736744384	40.0	37.0	41.0	33.0	41.0
15	37.999581737218755	40.0	37.0	41.0	33.0	41.0
16	38.118840187912205	40.0	37.0	41.0	33.0	41.0
17	38.058171681569405	40.0	37.0	41.0	33.0	41.0
18	37.85692862498661	39.0	37.0	41.0	33.0	41.0
19	37.62679483190427	39.0	37.0	41.0	33.0	41.0
20	37.32142219547154	39.0	35.0	41.0	33.0	41.0
21	37.188396778356434	39.0	35.0	41.0	33.0	41.0
22	37.22265096991058	39.0	35.0	41.0	33.0	41.0
23	37.14436951986493	39.0	35.0	40.0	33.0	41.0
24	37.03549877836663	39.0	35.0	40.0	33.0	41.0
25	36.93766609368066	38.0	35.0	40.0	33.0	41.0
26	36.869563221439535	38.0	35.0	40.0	32.0	41.0
27	36.788438094557996	38.0	35.0	40.0	32.0	41.0
28	36.63209197700575	38.0	35.0	40.0	32.0	41.0
29	36.47904350443002	38.0	35.0	40.0	31.0	41.0
30	36.335640579651006	38.0	35.0	40.0	31.0	41.0
31	36.09879162862346	38.0	35.0	40.0	30.0	41.0
32	35.788835954276735	38.0	35.0	40.0	30.0	41.0
33	35.45674040673505	38.0	35.0	40.0	27.0	41.0
34	35.119130931552824	38.0	35.0	40.0	24.0	41.0
35	34.85973659646313	38.0	34.0	40.0	23.0	41.0
36	34.69487730108289	38.0	34.0	40.0	23.0	41.0
37	34.577353110701914	38.0	34.0	40.0	22.0	41.0
38	34.455539176430385	38.0	34.0	40.0	21.0	41.0
39	34.33606139281506	37.0	34.0	40.0	21.0	41.0
40	34.16775653025519	37.0	33.0	40.0	21.0	41.0
41	34.03163749878857	37.0	33.0	40.0	20.0	41.0
42	33.92522787670429	37.0	33.0	40.0	19.0	41.0
43	33.798111696565655	37.0	33.0	40.0	18.0	41.0
44	33.6911741452392	36.0	33.0	40.0	18.0	41.0
45	33.652117582849186	36.0	33.0	40.0	20.0	41.0
46	33.491244535804825	36.0	33.0	40.0	20.0	41.0
47	33.339361588174384	35.0	33.0	40.0	18.0	41.0
48	33.24084540089468	35.0	33.0	40.0	18.0	41.0
49	33.227812944723006	35.0	33.0	40.0	18.0	41.0
50	33.118778978724706	35.0	33.0	40.0	18.0	41.0
51	32.07955409106907	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	6.0
12	10.0
13	15.0
14	24.0
15	20.0
16	40.0
17	82.0
18	181.0
19	298.0
20	476.0
21	782.0
22	1202.0
23	1993.0
24	3257.0
25	5424.0
26	7969.0
27	9072.0
28	9185.0
29	8955.0
30	9283.0
31	10544.0
32	12837.0
33	17776.0
34	28692.0
35	31195.0
36	37899.0
37	50195.0
38	74925.0
39	69730.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.860631780830303	5.333360537416667	37.58575662206897	30.22025105968406
2	32.915750654173195	5.1165779983575534	46.18488235084086	15.782788996628394
3	21.68820039887987	5.226244459293341	55.70163581553591	17.383919326290876
4	16.847063744268016	5.580747670225301	56.637626307708786	20.934562277797898
5	15.627980759912063	6.34815785849456	56.63507592489633	21.38878545669705
6	19.728231207504248	7.173461736606665	58.235696177996324	14.86261087789277
7	72.83000678401828	3.070405867920775	20.12099016062311	3.9785971874378343
8	73.74763451994144	3.235925712449439	18.844523562986804	4.171916204622314
9	68.62748598564644	5.2958699100735025	20.918239827798153	5.1584042764819
10	36.67501491973945	21.77338318481604	26.705313467551477	14.846288427893025
11	28.298027533932846	19.982249335625276	32.76400287683181	18.955720253610068
12	23.704533050410866	18.675433182520695	38.1312835056542	19.48875026141424
13	22.83587266448694	18.43340185361823	40.42229238608715	18.30843309580768
14	19.727976169223	19.414024045009157	42.34298568215089	18.515014103616952
15	17.89501604190789	18.061811077842783	42.9089156282358	21.134257252013526
16	21.092430973889183	18.229116190340168	39.54547077516335	21.132982060607297
17	21.046014006702407	19.128126131732373	38.01371085799979	21.812149003565434
18	22.738193002769716	17.627990961443313	39.09813362985784	20.53568240592913
19	21.633622206693225	21.19036567388765	36.884911425204926	20.2911006942142
20	23.104427974638995	23.89504664650164	34.85965243383032	18.14087294502905
21	21.794806400440706	24.15671592306005	36.024667302562115	18.023810373937128
22	21.1102836535764	20.68487980045805	36.734693877551024	21.47014266841453
23	19.53414707547603	22.916209723079433	35.98947201975016	21.560171181694372
24	19.563731516100567	22.998077011359406	36.26210794240215	21.176083530137873
25	20.973073058266046	23.71728496447317	33.281730587761224	22.027911389499565
26	20.12940642390423	22.4000122418375	34.14248478696653	23.32809654729175
27	19.772352830159807	21.199036975450014	36.125407423654295	22.903202770735888
28	18.523685405179318	22.929216675422982	34.87316946273636	23.673928456661343
29	20.30461772312024	22.65556059964601	33.8014986049406	23.238323072293152
30	23.250054833230468	21.05417523170228	34.79257736586261	20.903192569204638
31	23.49846211916409	23.407158414478012	31.17613453779412	21.918244928563777
32	23.785125147284607	22.62648623558396	30.257996725308466	23.330391891822963
33	24.47933934883626	22.564001856678686	28.562247193303712	24.394411601181336
34	22.672138087927	23.451024998852326	30.96572795576616	22.911108957454516
35	21.72798637075425	25.29826726991721	29.579594897194067	23.394151462134467
36	23.04984978245235	27.859616728470943	26.579324556615948	22.511208932460764
37	23.50585822932022	27.231457441762007	27.428346994883935	21.834337334033837
38	24.7134644910201	27.77800447847222	27.584430423006495	19.924100607501185
39	23.546154277757093	26.745354477707107	27.46175700972716	22.246734234808645
40	23.76523216134742	25.8014577988156	30.280440094058118	20.15286994577886
41	19.86697203250208	27.369943228478594	29.241924212824344	23.521160526194983
42	21.258460894980335	27.647169840193015	29.198567705012522	21.895801559814128
43	21.57394324888166	24.813694535549786	31.123851690138686	22.488510525429867
44	22.911619034017004	23.524731062132425	30.44468474718055	23.118965156670015
45	23.322230666823092	23.21409443557478	29.07589429173319	24.38778060586894
46	22.44999974496172	24.089640855092348	30.559451973741258	22.90090742620467
47	20.119204892654388	27.08685073629552	31.73007768466047	21.063866686389627
48	20.332671934057302	26.914699896454458	31.561497380756855	21.19113078873139
49	23.160026319950624	24.15748103790379	31.29498237685477	21.38751026529082
50	21.267642273105192	24.188340669934558	30.834893317486955	23.709123739473295
51	19.916449459063806	23.595121627756328	29.98638095578146	26.502047957398407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	1377.5
2	1883.0
3	10651.5
4	19420.0
5	11916.5
6	4413.0
7	4301.0
8	4189.0
9	4301.0
10	4413.0
11	4315.0
12	4217.0
13	4116.5
14	4016.0
15	3752.5
16	3489.0
17	3266.0
18	3043.0
19	2877.5
20	2712.0
21	2665.0
22	2618.0
23	2599.5
24	2581.0
25	2628.5
26	2712.5
27	2749.0
28	3060.5
29	3372.0
30	3779.5
31	4187.0
32	4881.0
33	5575.0
34	6219.0
35	6863.0
36	7348.0
37	7833.0
38	8359.0
39	8885.0
40	9563.5
41	10242.0
42	11505.5
43	12769.0
44	14603.0
45	16437.0
46	19786.5
47	23136.0
48	27048.5
49	30961.0
50	32693.5
51	34426.0
52	32743.5
53	31061.0
54	27914.5
55	24768.0
56	23162.5
57	21557.0
58	20423.0
59	19289.0
60	18031.5
61	16774.0
62	15088.5
63	13403.0
64	12329.5
65	11256.0
66	9493.5
67	7731.0
68	7017.0
69	6303.0
70	5254.5
71	4206.0
72	3603.5
73	3001.0
74	2593.0
75	1748.0
76	1311.0
77	1012.5
78	714.0
79	507.5
80	301.0
81	219.0
82	137.0
83	108.0
84	79.0
85	52.5
86	26.0
87	19.5
88	13.0
89	8.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	392098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.19630269034272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79002679133033	29.870773979444454
2	10.010980158597842	8.448526979984196
3	5.031999353387118	6.369953035593949
4	3.316273002200698	5.5973783761868905
5	2.294966458039054	4.841954966379982
6	1.7008380502206617	4.306144631661802
7	1.3163452235001036	3.8881431047058053
8	1.0540740744714567	3.5582422963552354
9	0.8387763498824995	3.185393467422848
>10	3.554881705486689	22.640703710810513
>50	0.0635871830184868	1.800006332388809
>100	0.02543487320739472	2.0233461300191067
>500	0.0012111844384473675	0.3605634490347259
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.055922192236838E-4	3.1088695400116766
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12166	3.102795729639019	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCC	787	0.20071512734061384	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	624	0.15914388749751338	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	458	0.11680753281067488	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	439	0.11196180546700059	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.11119669062326254	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	422	0.10762615468581833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11476722656070676	0.0
2	0.0	0.0	0.0	0.38893337890017293	0.0
3	0.0	0.0	0.0	0.5687353671786135	0.0
4	0.0	0.0	0.0	0.8625394671740229	0.0
5	0.0	0.0	0.0	1.5723110038816825	0.0
6	0.0	0.0	0.0	2.542986702304016	0.0
7	0.0	0.0	0.0	3.466480318695836	0.0
8	0.0	0.0	0.0	4.81665297961224	0.0
9	0.0	0.0	0.0	5.730455141316711	0.0
10	0.0	0.0	0.0	7.076292151451933	0.0
11	0.0	0.0	0.0	9.40096608500936	0.0
12	0.0	0.0	0.0	11.4048018607593	0.0
13	0.0	0.0	0.0	12.16507097715367	0.0
14	0.0	0.0	0.0	12.536151676366623	0.0
15	0.0	0.0	0.0	12.895245576361011	0.0
16	0.0	0.0	0.0	13.5647210646318	0.0
17	0.0	0.0	0.0	14.344883166963362	0.0
18	0.0	0.0	0.0	15.101326709139041	0.0
19	0.0	0.0	0.0	15.574932797412892	0.0
20	0.0	0.0	0.0	16.038847430999393	0.0
21	0.0	0.0	0.0	16.585649505990848	0.0
22	2.5503828124601505E-4	0.0	0.0	17.076853235670676	0.0
23	2.5503828124601505E-4	0.0	0.0	17.475478069258195	0.0
24	2.5503828124601505E-4	0.0	0.0	17.760355829409995	0.0
25	5.100765624920301E-4	0.0	0.0	17.949084157532045	0.0
26	5.100765624920301E-4	0.0	0.0	18.131946605185437	0.0
27	5.100765624920301E-4	0.0	0.0	18.330111349713594	0.0
28	5.100765624920301E-4	0.0	0.0	18.50557768721085	0.0
29	5.100765624920301E-4	0.0	0.0	18.708588159082677	0.0
30	5.100765624920301E-4	0.0	0.0	18.97612331610975	0.0
31	5.100765624920301E-4	0.0	0.0	19.15133461532576	0.0
32	5.100765624920301E-4	0.0	0.0	19.469877428602032	0.0
33	5.100765624920301E-4	0.0	0.0	19.684619661411176	0.0
34	7.65114843738045E-4	0.0	0.0	19.901657238751536	0.0
35	7.65114843738045E-4	0.0	0.0	20.185769884059596	0.0
36	7.65114843738045E-4	0.0	0.0	20.40586792077491	0.0
37	7.65114843738045E-4	0.0	0.0	20.60989854577172	0.0
38	7.65114843738045E-4	0.0	0.0	20.92538089967304	0.0
39	7.65114843738045E-4	0.0	0.0	21.58720523950645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	35	1.2091914E-7	45.000004	17
ATCAACC	55	1.8189894E-12	45.000004	10
CACAACG	30	2.161829E-6	45.000004	12
CGTTTTT	4900	0.0	43.484695	1
TGGGACG	65	0.0	41.538464	6
ACCTTCT	45	1.9226718E-8	40.000004	19
ATCTACC	40	3.4504592E-7	39.375004	15
TGGGAAC	390	0.0	39.23077	6
TTGGGAC	635	0.0	38.97638	5
CATATGC	110	0.0	38.86364	33
GTTTTTT	5775	0.0	38.727272	2
TACTCCC	35	6.2377603E-6	38.57143	25
TGTTATC	65	9.094947E-12	38.076927	24
CGGGATA	65	9.094947E-12	38.076927	6
GTTATCT	65	9.094947E-12	38.076927	25
TTTGGGC	1020	0.0	37.72059	4
TTTGGGA	1440	0.0	37.656254	4
GGCCGAT	180	0.0	37.500004	8
TAGCCGT	30	1.13855014E-4	37.500004	44
GACTAAT	330	0.0	37.5	9
>>END_MODULE