##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398420
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.154854173987246	33.0	31.0	34.0	30.0	34.0
2	32.330947241604335	34.0	31.0	34.0	30.0	34.0
3	32.468718939812256	34.0	31.0	34.0	31.0	34.0
4	35.94139601425631	37.0	35.0	37.0	35.0	37.0
5	35.97265950504493	37.0	35.0	37.0	35.0	37.0
6	35.951101852316654	37.0	35.0	37.0	35.0	37.0
7	35.940876462024995	37.0	35.0	37.0	35.0	37.0
8	35.83205913357763	37.0	35.0	37.0	35.0	37.0
9	37.68014406907284	39.0	38.0	39.0	35.0	39.0
10	37.22257918779178	39.0	37.0	39.0	34.0	39.0
11	37.138509612971234	39.0	37.0	39.0	34.0	39.0
12	36.939932734300484	39.0	37.0	39.0	33.0	39.0
13	36.86227348024698	39.0	37.0	39.0	33.0	39.0
14	37.87804076100598	40.0	37.0	41.0	33.0	41.0
15	37.98580141559159	40.0	37.0	41.0	33.0	41.0
16	38.149342402489836	40.0	37.0	41.0	33.0	41.0
17	38.136973545504745	40.0	37.0	41.0	33.0	41.0
18	37.97895938958887	39.0	37.0	41.0	33.0	41.0
19	37.77060639526128	39.0	37.0	41.0	34.0	41.0
20	37.43419507052859	39.0	35.0	41.0	33.0	41.0
21	37.33753074644847	39.0	35.0	41.0	33.0	41.0
22	37.34366999648612	39.0	35.0	41.0	33.0	41.0
23	37.218846945434464	39.0	35.0	41.0	33.0	41.0
24	37.124976155815474	39.0	35.0	41.0	33.0	41.0
25	37.01853069625019	39.0	35.0	41.0	33.0	41.0
26	36.985954520355406	39.0	35.0	41.0	33.0	41.0
27	36.90314241252949	38.0	35.0	40.0	33.0	41.0
28	36.821304151398024	38.0	35.0	40.0	33.0	41.0
29	36.61663320114452	38.0	35.0	40.0	32.0	41.0
30	36.38356006224587	38.0	35.0	40.0	31.0	41.0
31	36.15081321218814	38.0	35.0	40.0	30.0	41.0
32	35.92964459615481	38.0	35.0	40.0	30.0	41.0
33	35.54324833090708	38.0	35.0	40.0	27.0	41.0
34	35.217047336981075	38.0	35.0	40.0	24.0	41.0
35	35.014467145223634	38.0	34.0	40.0	23.0	41.0
36	34.83932031524522	38.0	34.0	40.0	23.0	41.0
37	34.75761758947844	38.0	34.0	40.0	23.0	41.0
38	34.6148612017469	38.0	34.0	40.0	22.0	41.0
39	34.549028663219715	38.0	34.0	40.0	22.0	41.0
40	34.388308819838365	37.0	34.0	40.0	21.0	41.0
41	34.26054414939009	37.0	34.0	40.0	21.0	41.0
42	34.085603132372874	37.0	33.0	40.0	20.0	41.0
43	33.994681491892976	37.0	33.0	40.0	20.0	41.0
44	33.876414336629686	37.0	33.0	40.0	18.0	41.0
45	33.807738065358166	36.0	33.0	40.0	20.0	41.0
46	33.63712163044024	36.0	33.0	40.0	20.0	41.0
47	33.57016465036896	36.0	33.0	40.0	20.0	41.0
48	33.41483359269113	35.0	33.0	40.0	19.0	41.0
49	33.39451081773004	35.0	33.0	40.0	20.0	41.0
50	33.232889915164904	35.0	33.0	40.0	18.0	41.0
51	32.32996586516741	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	10.0
11	9.0
12	15.0
13	17.0
14	27.0
15	34.0
16	62.0
17	90.0
18	162.0
19	308.0
20	441.0
21	797.0
22	1288.0
23	1961.0
24	3176.0
25	5335.0
26	7894.0
27	9076.0
28	9216.0
29	8771.0
30	9156.0
31	10391.0
32	12716.0
33	17301.0
34	28824.0
35	31039.0
36	37707.0
37	49232.0
38	76213.0
39	77113.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.892726268761606	5.524823051051654	37.535515285377244	30.0469353948095
2	33.10501480849355	5.149841875407861	45.894031424125295	15.851111891973293
3	21.82044074092666	5.27282766929371	55.34787410270569	17.558857487073944
4	17.121881431655037	5.647557853521409	56.339290196275286	20.891270518548268
5	15.775814467145224	6.338537222027006	56.4710606897244	21.414587621103358
6	19.839114502284023	7.2872847748607	58.06887204457608	14.804728678279202
7	72.78600471863862	3.104763817077456	19.95155865669394	4.1576728075899805
8	73.65016816424878	3.2681592289543695	18.843682546056925	4.237990060739922
9	68.55353646905276	5.300436725064003	20.775312484313034	5.370714321570203
10	36.72280508006626	21.807389187289793	26.72757391697204	14.742231815671905
11	28.477486069976404	19.92520455800412	32.556347572913005	19.04096179910647
12	23.8758094473169	18.733999297224035	37.89368003614276	19.496511219316297
13	23.00788113046534	18.5128758596456	40.17619597409768	18.303047035791376
14	20.04141358365544	19.46262737814367	42.04332111841775	18.452637919783143
15	18.06862105315998	18.082927563877316	42.65674413935044	21.191707243612267
16	21.196225089101954	18.35098639626525	39.43828121078259	21.01450730385021
17	21.179408664223683	19.006575975101654	38.04753777420812	21.766477586466543
18	22.876110637016215	17.636162843230764	38.74278399678731	20.744942522965715
19	21.684905376236134	21.06470558706892	36.88820842327193	20.36218061342302
20	23.417750112946138	23.84117263189599	34.576577481050144	18.164499774107725
21	21.901008985492695	24.17398724963606	35.76226093067617	18.16274283419507
22	21.373676020280104	20.463832136940916	36.54209126047889	21.620400582300086
23	19.82957682847247	22.790522564128306	35.80442748857989	21.575473118819335
24	19.828070879975904	22.918277194919934	36.060689724411425	21.192962200692737
25	21.093820591335778	23.644144370262538	33.200893529441295	22.061141508960393
26	20.372471261482858	22.37739069323829	34.01134481200743	23.23879323327142
27	19.996737111590786	21.168114050499472	35.94498268159229	22.890166156317456
28	18.641634456101603	22.81386476582501	34.85442497866573	23.690075799407662
29	20.486923347221524	22.606294864715625	33.6609607951408	23.24582099292204
30	23.34395863661463	21.104362230811706	34.720395562471765	20.831283570101903
31	23.701119421715777	23.40394558506099	30.963054063551027	21.931880929672204
32	23.8484513829627	22.537272225289897	30.16163847196426	23.452637919783143
33	24.501782039054266	22.490838813312585	28.653682044074092	24.353697103559057
34	22.802319160684707	23.391145022840217	30.96255208071884	22.843983735756236
35	21.809899101450732	25.25952512424075	29.574318558305308	23.35625721600321
36	23.04603182571156	27.84624265850108	26.661312183123336	22.446413332664022
37	23.541488881080266	27.122132423071132	27.4122785000753	21.924100195773306
38	24.75955022338236	27.653230259525124	27.630390040660608	19.956829476431906
39	23.61979820290146	26.479343406455495	27.64595150845841	22.25490688218463
40	23.71818683801014	25.892525475628737	30.18121580241956	20.20807188394157
41	20.038401686662315	27.226795843582153	29.195823502836205	23.53897896691933
42	21.259725917373625	27.566136238140654	29.235982129411177	21.938155715074544
43	21.63244817027258	24.75879724913408	31.0305707544802	22.578183826113147
44	22.94187038803273	23.4074594648863	30.49144119271121	23.15922895436976
45	23.26263741780031	23.26514733196125	29.05878218964911	24.41343306058933
46	22.524973645901312	23.99804226695447	30.62722754881783	22.84975653832639
47	20.241955725114202	27.019727925304952	31.74589629034687	20.992420059233975
48	20.470106922343255	26.85156367652226	31.491641985844083	21.186687415290397
49	23.212941117413784	24.203604236735103	31.28457406756689	21.298880578284223
50	21.328497565383266	24.252045580041163	30.771045630239446	23.648411224336126
51	20.12725264795944	23.57487073942071	29.94904874253301	26.348827870086843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	721.0
1	1257.0
2	1793.0
3	10610.5
4	19428.0
5	12148.0
6	4868.0
7	4556.0
8	4244.0
9	4368.0
10	4492.0
11	4446.5
12	4401.0
13	4153.5
14	3906.0
15	3682.5
16	3459.0
17	3209.5
18	2960.0
19	2843.0
20	2726.0
21	2709.5
22	2693.0
23	2627.5
24	2562.0
25	2618.0
26	2808.5
27	2943.0
28	3189.5
29	3436.0
30	3778.0
31	4120.0
32	4865.0
33	5610.0
34	6233.0
35	6856.0
36	7285.0
37	7714.0
38	8380.5
39	9047.0
40	9661.0
41	10275.0
42	11598.0
43	12921.0
44	14928.5
45	16936.0
46	20265.5
47	23595.0
48	27524.5
49	31454.0
50	33052.5
51	34651.0
52	32963.0
53	31275.0
54	28179.5
55	25084.0
56	23552.0
57	22020.0
58	20957.5
59	19895.0
60	18439.0
61	16983.0
62	15453.5
63	13924.0
64	12763.5
65	11603.0
66	9815.0
67	8027.0
68	7298.5
69	6570.0
70	5396.0
71	4222.0
72	3699.0
73	3176.0
74	2785.0
75	1923.5
76	1453.0
77	1083.0
78	713.0
79	521.0
80	329.0
81	238.0
82	147.0
83	106.5
84	66.0
85	46.0
86	26.0
87	24.0
88	22.0
89	11.5
90	1.0
91	1.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.31833842884266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64114302556703	29.894157975562237
2	10.21468104327676	8.64536658664139
3	5.095090756909863	6.468473249297389
4	3.2346122704931672	5.47533666995268
5	2.2618020236229897	4.785785174735943
6	1.7223295036267887	4.373167369227542
7	1.3741359107181317	4.0705803961897065
8	1.0211279294443187	3.456994983789443
9	0.8698102682333034	3.3128032769981415
>10	3.477249396012466	22.37337729893824
>50	0.05987610346616032	1.6685449729702828
>100	0.026944246559772136	2.20065498712937
>500	5.98761034661603E-4	0.2120838485005507
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.98761034661603E-4	3.0626732100670924
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12087	3.033733246322976	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCC	837	0.21007981527031774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.1222328196375684	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	442	0.11093820591335778	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	433	0.10867928316851563	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	422	0.10591837759148637	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	417	0.10466342051101851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11194217157773204	0.0
2	0.0	0.0	0.0	0.36770242457707947	0.0
3	0.0	0.0	0.0	0.5592088750564731	0.0
4	0.0	0.0	0.0	0.8292756387731539	0.0
5	0.0	0.0	0.0	1.529039706842026	0.0
6	0.0	0.0	0.0	2.476783294011345	0.0
7	0.0	0.0	0.0	3.3778424777872598	0.0
8	0.0	0.0	0.0	4.690527583956628	0.0
9	2.509914160935696E-4	0.0	0.0	5.581547111088801	0.0
10	2.509914160935696E-4	0.0	0.0	6.8794237237086495	0.0
11	2.509914160935696E-4	0.0	0.0	9.152150996435921	0.0
12	2.509914160935696E-4	0.0	0.0	11.124943526931379	0.0
13	5.019828321871392E-4	0.0	0.0	11.837006174388836	0.0
14	5.019828321871392E-4	0.0	0.0	12.180111440188746	0.0
15	5.019828321871392E-4	0.0	0.0	12.523216705988656	0.0
16	5.019828321871392E-4	0.0	0.0	13.18934792430099	0.0
17	5.019828321871392E-4	0.0	0.0	13.934541438682798	0.0
18	5.019828321871392E-4	0.0	0.0	14.668942322172581	0.0
19	5.019828321871392E-4	0.0	0.0	15.108930274584608	0.0
20	7.529742482807088E-4	0.0	0.0	15.574770342854274	0.0
21	7.529742482807088E-4	0.0	0.0	16.090055720094373	0.0
22	7.529742482807088E-4	0.0	0.0	16.569951307665278	0.0
23	7.529742482807088E-4	0.0	0.0	16.955474122785002	0.0
24	0.0010039656643742784	0.0	0.0	17.222528989508557	0.0
25	0.0010039656643742784	0.0	0.0	17.416545354148887	0.0
26	0.0010039656643742784	0.0	0.0	17.586968525676422	0.0
27	0.0010039656643742784	0.0	0.0	17.782490838813313	0.0
28	0.0010039656643742784	0.0	0.0	17.950906079012096	0.0
29	0.0010039656643742784	0.0	0.0	18.14517343506852	0.0
30	0.0010039656643742784	0.0	0.0	18.396666833994278	0.0
31	0.0010039656643742784	0.0	0.0	18.57963957632649	0.0
32	0.0010039656643742784	0.0	0.0	18.88559811254455	0.0
33	0.0010039656643742784	0.0	0.0	19.09366999648612	0.0
34	0.0010039656643742784	0.0	0.0	19.308518648662215	0.0
35	0.0010039656643742784	0.0	0.0	19.57180864414437	0.0
36	0.0010039656643742784	0.0	0.0	19.802720746950456	0.0
37	0.0010039656643742784	0.0	0.0	20.002258922744844	0.0
38	0.0010039656643742784	0.0	0.0	20.324280909592893	0.0
39	0.0010039656643742784	0.0	0.0	20.98790221374429	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGT	20	7.027731E-4	45.000004	25
GCGTTAG	20	7.027731E-4	45.000004	1
GTTACGT	20	7.027731E-4	45.000004	22
TTACGTT	20	7.027731E-4	45.000004	23
TATCCGG	25	3.8862017E-5	45.0	2
CGTTTTT	4540	0.0	43.56278	1
TTTGTCG	65	0.0	41.53846	1
CGGGTAC	60	3.6379788E-12	41.250004	6
GCGATCT	95	0.0	40.263157	9
GCTACGA	95	0.0	40.263157	10
AGCTACG	95	0.0	40.263157	9
TGGGCGA	605	0.0	40.16529	6
CGATGCG	45	1.9228537E-8	40.0	10
AACACGT	175	0.0	39.857143	41
AAACACG	195	0.0	39.23077	40
ACACGCG	495	0.0	39.090908	36
TTTGGGC	1135	0.0	39.052868	4
CACGTGA	180	0.0	38.75	43
TTACGGG	70	0.0	38.571426	3
TTGGGAT	605	0.0	38.305786	5
>>END_MODULE