##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241018
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1684894904115	33.0	31.0	34.0	30.0	34.0
2	32.35467890365035	34.0	31.0	34.0	30.0	34.0
3	32.43071056933507	34.0	31.0	34.0	31.0	34.0
4	35.99016256047266	37.0	35.0	37.0	35.0	37.0
5	36.024666207503174	37.0	35.0	37.0	35.0	37.0
6	35.99332000099577	37.0	35.0	37.0	35.0	37.0
7	36.064746201528514	37.0	35.0	37.0	35.0	37.0
8	35.92439153922113	37.0	35.0	37.0	35.0	37.0
9	37.73995718162129	39.0	38.0	39.0	35.0	39.0
10	37.107029350504945	39.0	37.0	39.0	33.0	39.0
11	37.10422872980441	39.0	37.0	39.0	34.0	39.0
12	37.165688869710976	39.0	37.0	39.0	34.0	39.0
13	37.134662141416825	39.0	37.0	39.0	33.0	39.0
14	38.22841862433511	40.0	38.0	41.0	33.0	41.0
15	38.27663079106125	40.0	38.0	41.0	33.0	41.0
16	38.3611970890141	40.0	38.0	41.0	34.0	41.0
17	38.35111070542449	40.0	38.0	41.0	34.0	41.0
18	38.03211793310043	39.0	37.0	40.0	34.0	41.0
19	37.64703881037931	39.0	37.0	40.0	34.0	41.0
20	37.175414284410294	38.0	35.0	40.0	33.0	41.0
21	37.05983785443411	38.0	35.0	40.0	33.0	41.0
22	37.07790704428715	38.0	35.0	40.0	33.0	41.0
23	37.00178824818063	38.0	35.0	40.0	33.0	41.0
24	36.89575882299248	38.0	35.0	40.0	33.0	41.0
25	36.815071903343316	38.0	35.0	40.0	33.0	41.0
26	36.74767029848393	38.0	35.0	40.0	33.0	41.0
27	36.68851289115336	38.0	35.0	40.0	33.0	41.0
28	36.57117725647047	38.0	35.0	40.0	32.0	41.0
29	36.46862060095096	38.0	35.0	40.0	32.0	41.0
30	36.2867586653279	38.0	35.0	40.0	31.0	41.0
31	35.898376884714004	38.0	35.0	40.0	30.0	41.0
32	35.36279862914803	38.0	35.0	40.0	27.0	41.0
33	34.75859479374984	38.0	35.0	40.0	21.0	41.0
34	34.26020878108689	38.0	34.0	40.0	18.0	41.0
35	33.88444016629463	38.0	34.0	40.0	15.0	41.0
36	33.63311453916305	38.0	33.0	40.0	12.0	41.0
37	33.41283638566414	38.0	33.0	40.0	10.0	41.0
38	33.30408517206184	38.0	33.0	40.0	10.0	41.0
39	33.25084433527786	38.0	33.0	40.0	10.0	41.0
40	33.100743512932645	38.0	33.0	40.0	10.0	41.0
41	32.89990374162926	37.0	32.0	40.0	10.0	41.0
42	32.675202681957366	37.0	32.0	40.0	10.0	41.0
43	32.49160228696612	37.0	31.0	40.0	10.0	41.0
44	32.42558647072003	37.0	31.0	40.0	10.0	41.0
45	32.410234920213426	37.0	31.0	40.0	10.0	41.0
46	32.148532474752926	36.0	31.0	40.0	9.0	41.0
47	31.88987544498751	36.0	30.0	40.0	8.0	41.0
48	31.75822137765644	36.0	30.0	40.0	8.0	41.0
49	31.744475516351475	36.0	30.0	40.0	8.0	41.0
50	31.640624351708173	36.0	30.0	40.0	8.0	41.0
51	30.62327709963571	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	3.0
14	8.0
15	7.0
16	16.0
17	39.0
18	101.0
19	187.0
20	329.0
21	524.0
22	922.0
23	1574.0
24	2874.0
25	5346.0
26	8099.0
27	8379.0
28	7157.0
29	6413.0
30	5951.0
31	6311.0
32	7281.0
33	9534.0
34	14034.0
35	17560.0
36	22413.0
37	28768.0
38	47239.0
39	39932.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.572928163041766	4.9527421188458955	39.699109610070614	31.775220108041722
2	40.259233750176335	4.620401795716503	39.19997676522085	15.920387688886306
3	18.20776871436988	4.834078782497573	59.36735015641985	17.590802346712696
4	14.299346936743316	4.8830377814105175	60.002987328747224	20.814627953098938
5	13.092383141508105	6.082533254777651	60.43988415802969	20.385199445684556
6	16.782563957878665	6.742235019791052	63.782788007534705	12.692413014795575
7	64.42589350173016	2.3840543029981163	29.115252802695235	4.074799392576487
8	64.48688479698612	2.3736816337368993	28.172999527006283	4.966434042270702
9	59.471491755802475	4.774332207552963	30.429262544706205	5.324913491938362
10	27.67428158892697	19.69230513903526	37.27024537586404	15.36316789617373
11	20.018421860607923	20.29848393066078	41.48569816362264	18.197396045108665
12	17.06677509563601	18.626409645752602	46.27164776074816	18.03516749786323
13	19.618866640665843	19.234662971230364	47.594785451709	13.551684936394793
14	17.55719489830635	20.021741114771512	47.47736683567202	14.943697151250115
15	14.405563069978175	19.787318789468006	48.43746110249027	17.369657038063547
16	15.131649918263365	19.05334871254429	46.63801043905435	19.176990930138
17	14.760723265482245	19.07575367814852	45.28997834186658	20.87354471450265
18	16.37678513637986	17.828543926179787	47.49894198773536	18.295728949705
19	16.21497149590487	21.48719182799625	46.33969247110174	15.958144204997138
20	17.150586263266643	23.51484121517895	43.984681642035035	15.349890879519373
21	15.412126895086676	24.600237326672698	45.057630550415325	14.930005227825308
22	14.159523355102108	22.550182973885768	43.57309412574995	19.71719954526218
23	13.2662290783261	23.622716975495607	42.368619771137425	20.74243417504087
24	13.91348364022604	23.80444614095213	42.88517870034603	19.396891518475798
25	13.934643885518923	24.771178916097554	40.85462496577019	20.43955223261333
26	13.720551991967406	25.29728070102648	42.46819739604511	18.513969910961006
27	14.767361773809426	23.69366603324233	43.88054004265241	17.658432150295827
28	13.188226605481749	22.456414043764365	43.746940062567944	20.608419288185946
29	14.593930743761877	21.589258893526626	43.607531387697186	20.209278975014314
30	19.527587151167133	21.553991818038487	41.51764598494718	17.400775045847197
31	18.511895377108765	25.355367648889292	38.78258055414948	17.350156419852457
32	17.338953937050345	24.104008829216074	38.854774332207555	19.702262901526026
33	19.687741164560325	22.472595407811866	36.521338655204175	21.318324772423637
34	18.099478047282773	21.878034005758906	37.924553352861615	22.097934594096706
35	19.226364835821393	22.16473458413894	38.16022039847646	20.4486801815632
36	22.79041399397555	24.25752433428209	35.19612643039109	17.755935241351267
37	21.424125998888048	24.763295687459028	36.98893858549984	16.823639728153083
38	19.542523794903286	24.304823706113236	37.65693848592221	18.495714013061264
39	20.05617837671875	22.87298044129484	34.43062343891328	22.640217743073134
40	24.63384477507904	22.399986722983346	33.005833589192505	19.960334912745108
41	24.735911840609415	24.481988897094823	31.475657419777775	19.306441842517984
42	23.227310823258012	27.348579774124754	33.36223850500792	16.061870897609307
43	19.51555485482412	25.32964342912148	37.510061489183386	17.644740226871022
44	18.942568604834495	24.038038652714736	35.08410160236995	21.935291140080825
45	21.72991228870873	21.55565144512028	33.70370677708719	23.010729489083804
46	23.148063630102317	22.46969106041872	34.182509190184966	20.199736119293995
47	19.500618211087968	26.780987312150963	35.63219344613265	18.08620103062842
48	18.69237982225394	26.420018421860608	36.550382129135585	18.33721962674987
49	21.219162054286404	23.723539320714636	35.582819540449265	19.4744790845497
50	19.637537445336033	22.08673211129459	34.07006945539337	24.205660987976003
51	18.855023276269822	21.882183073463395	31.85944618244281	27.40334746782398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409.0
1	696.0
2	983.0
3	8332.5
4	15682.0
5	10014.5
6	4347.0
7	4262.0
8	4177.0
9	4240.0
10	4303.0
11	4178.5
12	4054.0
13	3924.0
14	3794.0
15	3616.5
16	3439.0
17	3327.5
18	3216.0
19	3071.0
20	2926.0
21	2798.0
22	2670.0
23	2630.5
24	2591.0
25	2634.0
26	2610.5
27	2544.0
28	2737.5
29	2931.0
30	3073.0
31	3215.0
32	3523.0
33	3831.0
34	4256.0
35	4681.0
36	5061.0
37	5441.0
38	5979.5
39	6518.0
40	7396.0
41	8274.0
42	9765.0
43	11256.0
44	12716.5
45	14177.0
46	17306.5
47	20436.0
48	23426.5
49	26417.0
50	25972.5
51	25528.0
52	21583.0
53	17638.0
54	13865.0
55	10092.0
56	8094.0
57	6096.0
58	5280.5
59	4465.0
60	3924.5
61	3384.0
62	2945.5
63	2507.0
64	2159.0
65	1811.0
66	1593.0
67	1375.0
68	1107.5
69	840.0
70	750.0
71	660.0
72	587.5
73	515.0
74	466.5
75	385.5
76	353.0
77	294.0
78	235.0
79	143.5
80	52.0
81	43.0
82	34.0
83	26.0
84	18.0
85	11.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.04197386587334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49840972442189	34.82966705298054
2	12.491213416083028	12.002050969770146
3	5.2611579478916575	7.5826923791052865
4	2.8955109251476716	5.564242407771807
5	1.8183426378405068	4.367838474316841
6	1.2354229021347303	3.561129286659486
7	0.840701710824991	2.827227873431446
8	0.6025396832408111	2.315775657232532
9	0.4326267604103711	1.8705819165581246
>10	1.8243148998767955	14.186218952185136
>50	0.06420158899301666	2.063206980195632
>100	0.03061921936590025	2.3521888226379364
>500	0.002963150261216153	0.8584041920823133
>1k	9.877167537387177E-4	0.5827088711316091
>5k	0.0	0.0
>10k+	9.877167537387177E-4	5.036066163941178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10613	4.403405554771843	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	1228	0.5095055141109792	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	652	0.2705192143325395	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	627	0.2601465450713225	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	530	0.2199005883378005	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	406	0.16845214880216416	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	365	0.15144097121376826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	266	0.11036520093934893	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.10538631969396477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.287945298691384	0.0
2	0.0	0.0	0.0	0.8995178783327387	0.0
3	0.0	0.0	0.0	1.3268718518948792	0.0
4	0.0	0.0	0.0	1.9633388377631547	0.0
5	0.0	0.0	0.0	3.3960119161224473	0.0
6	0.0	0.0	0.0	4.930752060012115	0.0
7	0.0	0.0	0.0	6.261358072841032	0.0
8	0.0	0.0	0.0	8.249591316831108	0.0
9	0.0	0.0	0.0	9.250346447153325	0.0
10	0.0	0.0	0.0	11.289198317138139	0.0
11	0.0	0.0	0.0	16.01955040702354	0.0
12	0.0	0.0	0.0	19.670729986971928	0.0
13	0.0	0.0	0.0	21.112531014281092	0.0
14	0.0	0.0	0.0	21.67846384917309	0.0
15	0.0	0.0	0.0	22.35144263084085	0.0
16	0.0	0.0	0.0	23.9704088491316	0.0
17	0.0	0.0	0.0	26.04867686230904	0.0
18	0.0	0.0	0.0	27.97259955687957	0.0
19	0.0	0.0	0.0	29.070442871486776	0.0
20	0.0	0.0	0.0	30.0226539096665	0.0
21	0.0	0.0	0.0	31.1229866648964	0.0
22	0.0	0.0	0.0	32.07519770307612	0.0
23	0.0	0.0	0.0	32.875967770042074	0.0
24	4.1490677044868016E-4	0.0	0.0	33.33029068368338	0.0
25	4.1490677044868016E-4	0.0	0.0	33.669269515139945	0.0
26	4.1490677044868016E-4	0.0	0.0	33.944767610717875	0.0
27	4.1490677044868016E-4	0.0	0.0	34.2331278161797	0.0
28	4.1490677044868016E-4	0.0	0.0	34.48871038677609	0.0
29	4.1490677044868016E-4	0.0	0.0	34.75591034694504	0.0
30	4.1490677044868016E-4	0.0	0.0	35.01149291754143	0.0
31	4.1490677044868016E-4	0.0	0.0	35.26168170012198	0.0
32	4.1490677044868016E-4	0.0	0.0	35.57742575243343	0.0
33	4.1490677044868016E-4	0.0	0.0	35.82512509439129	0.0
34	4.1490677044868016E-4	0.0	0.0	36.04917475043358	0.0
35	4.1490677044868016E-4	0.0	0.0	36.31512999029118	0.0
36	4.1490677044868016E-4	0.0	0.0	36.52507281613821	0.0
37	4.1490677044868016E-4	0.0	0.0	36.75161191280319	0.0
38	4.1490677044868016E-4	0.0	0.0	37.02005659328349	0.0
39	4.1490677044868016E-4	0.0	0.0	37.418367092914224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	35	1.2065902E-7	45.000004	5
CTGTCGG	35	1.2065902E-7	45.000004	2
CGCGGGA	45	3.8198777E-10	45.000004	4
GCGCGAC	35	1.2065902E-7	45.000004	9
CGGGAGG	45	3.8198777E-10	45.000004	6
CGGGTCC	20	7.0221827E-4	45.0	6
CGTGGCA	25	3.881604E-5	45.0	15
TCTTGCG	40	6.7757355E-9	45.0	1
CCGGGAG	20	7.0221827E-4	45.0	5
ACACGCT	20	7.0221827E-4	45.0	42
GGCACGT	25	3.881604E-5	45.0	11
TTGCTCG	20	7.0221827E-4	45.0	1
GGGTACG	25	3.881604E-5	45.0	7
GGCAACG	25	3.881604E-5	45.0	8
GCACGTG	25	3.881604E-5	45.0	12
TACGGGG	20	7.0221827E-4	45.0	4
TACGGGA	20	7.0221827E-4	45.0	4
ACGGGGG	20	7.0221827E-4	45.0	5
AGGCACG	25	3.881604E-5	45.0	10
GCGGGTC	20	7.0221827E-4	45.0	5
>>END_MODULE