##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241151
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09920755045594	33.0	31.0	34.0	30.0	34.0
2	32.301454275536905	34.0	31.0	34.0	30.0	34.0
3	32.39816131801236	34.0	31.0	34.0	30.0	34.0
4	35.92508843007079	37.0	35.0	37.0	35.0	37.0
5	36.00377771603684	37.0	35.0	37.0	35.0	37.0
6	35.98949620777024	37.0	35.0	37.0	35.0	37.0
7	35.99364298717401	37.0	35.0	37.0	35.0	37.0
8	35.89544310411319	37.0	35.0	37.0	35.0	37.0
9	37.71441130246194	39.0	38.0	39.0	35.0	39.0
10	37.1362797583257	39.0	37.0	39.0	33.0	39.0
11	37.11824541469868	39.0	37.0	39.0	33.0	39.0
12	37.16040986767627	39.0	37.0	39.0	34.0	39.0
13	37.137926029748996	39.0	37.0	39.0	33.0	39.0
14	38.27381599081074	40.0	38.0	41.0	33.0	41.0
15	38.29598882028273	40.0	38.0	41.0	33.0	41.0
16	38.451003727954685	40.0	38.0	41.0	34.0	41.0
17	38.46511936504514	40.0	38.0	41.0	34.0	41.0
18	38.18144647959163	39.0	38.0	40.0	34.0	41.0
19	37.84750633420554	39.0	37.0	41.0	34.0	41.0
20	37.33079688659802	39.0	35.0	40.0	34.0	41.0
21	37.253695817143615	39.0	35.0	40.0	33.0	41.0
22	37.256971772872596	38.0	35.0	40.0	33.0	41.0
23	37.14120613225738	38.0	35.0	40.0	33.0	41.0
24	37.0294379869874	38.0	35.0	40.0	33.0	41.0
25	36.95413247301483	38.0	35.0	40.0	33.0	41.0
26	36.94342134181488	38.0	35.0	40.0	33.0	41.0
27	36.894381528585825	38.0	35.0	40.0	33.0	41.0
28	36.828704836388816	38.0	35.0	40.0	33.0	41.0
29	36.65201471277332	38.0	35.0	40.0	33.0	41.0
30	36.37364970495665	38.0	35.0	40.0	31.0	41.0
31	35.977503721734514	38.0	35.0	40.0	30.0	41.0
32	35.52346869803567	38.0	35.0	40.0	27.0	41.0
33	34.875584177548504	38.0	35.0	40.0	21.0	41.0
34	34.39073858287961	38.0	34.0	40.0	18.0	41.0
35	34.04100335474454	38.0	34.0	40.0	15.0	41.0
36	33.75460603522274	38.0	34.0	40.0	13.0	41.0
37	33.580677666690164	38.0	33.0	40.0	12.0	41.0
38	33.459218497953565	38.0	33.0	40.0	10.0	41.0
39	33.44995459276553	38.0	33.0	40.0	10.0	41.0
40	33.31150606881166	38.0	33.0	40.0	10.0	41.0
41	33.138954431041135	38.0	33.0	40.0	10.0	41.0
42	32.85314180741527	37.0	32.0	40.0	10.0	41.0
43	32.71569265729771	37.0	32.0	40.0	10.0	41.0
44	32.6263959096168	37.0	32.0	40.0	10.0	41.0
45	32.579690733192066	37.0	32.0	40.0	10.0	41.0
46	32.31179219659052	37.0	31.0	40.0	10.0	41.0
47	32.16599143275375	36.0	31.0	40.0	10.0	41.0
48	32.01901298356631	36.0	31.0	40.0	8.0	41.0
49	31.993298804483498	36.0	31.0	40.0	8.0	41.0
50	31.80981625620462	36.0	30.0	40.0	8.0	41.0
51	30.89993406620748	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	11.0
12	4.0
13	11.0
14	10.0
15	12.0
16	20.0
17	49.0
18	99.0
19	192.0
20	290.0
21	545.0
22	863.0
23	1448.0
24	2916.0
25	5235.0
26	7886.0
27	8175.0
28	7150.0
29	6056.0
30	5996.0
31	6181.0
32	7051.0
33	8991.0
34	13487.0
35	17206.0
36	21247.0
37	27433.0
38	46698.0
39	45875.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.751508391008123	4.97945270805429	39.522125141508845	31.74691375942874
2	40.285961907684396	4.583435274993676	39.04026937479007	16.090333442531858
3	18.342449336722634	4.784139398136437	59.223474088848896	17.64993717629203
4	14.545658114625276	4.873709833258	59.885300081691554	20.69533197042517
5	13.215371281893917	6.007854000190751	60.15774348851964	20.61903122939569
6	16.792797873531523	6.73561378555345	63.63937947593001	12.83220886498501
7	64.54213335213207	2.4217191718052176	28.90097905461723	4.135168421445484
8	64.70178435917745	2.4059614100708684	27.95219592703327	4.940058303718417
9	59.600831014592515	4.8218750907108	30.258219953473137	5.319073941223549
10	27.638284726167424	19.833216532380128	37.13565359463573	15.392845146816725
11	20.083267330427823	20.328756671131366	41.39190797467147	18.196068023769342
12	17.247699574125754	18.7227090080489	46.087306293567096	17.942285124258245
13	19.65656372977927	19.46000638604028	47.29318974418518	13.590240139995272
14	17.74572778051926	20.145054343544086	47.22932934136703	14.879888534569627
15	14.408814394300668	19.98913543796211	48.178941824831746	17.423108342905483
16	15.129109976736569	19.188807013033326	46.526035554486604	19.156047455743497
17	15.04244228719765	19.20746752035032	45.06553984847668	20.684550343975353
18	16.63522025618803	17.901646686101238	47.24259903545911	18.22053402225162
19	16.393463016947887	21.608867473077034	46.03588622896028	15.9617832810148
20	17.40113041206547	23.69179476759375	43.67968617173472	15.22738864860606
21	15.510613681883964	24.811010528672906	44.72135715796327	14.957018631479862
22	14.235893693163204	22.67334574602635	43.457833473632704	19.63292708717774
23	13.644148272244362	23.567806063420843	42.137913589410786	20.65013207492401
24	14.173277324166186	23.987045461142603	42.496195329897034	19.343481884794176
25	14.180326849152605	24.908045166721266	40.542647552778135	20.368980431347993
26	13.990404352459663	25.310697446827923	42.19182172165987	18.507076479052547
27	14.984387375544783	23.71916351165867	43.454101372169305	17.842347740627243
28	13.3360425625438	22.614876156433105	43.57311394105768	20.475967339965415
29	14.782853896521267	21.474926498335066	43.478982048591966	20.263237556551704
30	19.684761829724945	21.605550049554015	41.396884109956	17.31280401076504
31	18.916778284145618	25.232323316096554	38.58744106389773	17.263457335860107
32	17.488627457485144	24.145037756426472	38.637617094683414	19.72871769140497
33	19.74945158842385	22.641000866676894	36.157428333284955	21.452119211614303
34	18.47970773498762	22.028936226679548	37.46449320135517	22.026862836977664
35	19.41480649053912	22.403390406840526	37.86465741381956	20.317145688800792
36	22.90639474851856	24.387209673606993	34.89722207247741	17.809173505397034
37	21.60513537161364	24.96402668867224	36.89638442303785	16.534453516676272
38	19.558285057909774	24.356108828078675	37.59179932905109	18.49380678496046
39	20.04760502755535	22.815994957516246	34.459322167438664	22.677077847489745
40	24.750467549377774	22.44983433616282	32.968969649721544	19.830728464737863
41	24.687851180380758	24.58501105116711	31.49603360550029	19.231104162951844
42	23.247674693449333	27.375378911968017	33.24182773449001	16.13511866009264
43	19.563675871134684	25.382022052572868	37.58806722758769	17.466234848704755
44	19.28418293932018	23.88379065398858	35.03821257220579	21.793813834485448
45	21.884213625487764	21.513906224730565	33.4956106340011	23.10626951578057
46	23.207036255292326	22.727668556215814	34.037594702074635	20.027700486417224
47	19.575286853465258	26.949919345140593	35.6361781622303	17.838615639163844
48	18.54398281574615	26.5999311634619	36.40706445339227	18.44902156739968
49	21.191701465057164	23.926087803907095	35.517580271282306	19.364630459753432
50	19.76479467221782	22.274010889442714	33.931851827278344	24.029342611061118
51	18.702804466910774	22.09860212066299	31.680150611027948	27.518442801398297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	684.0
2	1044.0
3	8344.5
4	15645.0
5	10022.0
6	4399.0
7	4153.5
8	3908.0
9	4075.5
10	4243.0
11	4161.0
12	4079.0
13	3926.0
14	3773.0
15	3608.5
16	3444.0
17	3286.0
18	3128.0
19	3015.5
20	2903.0
21	2794.0
22	2685.0
23	2643.0
24	2601.0
25	2573.5
26	2564.0
27	2582.0
28	2692.5
29	2803.0
30	3085.5
31	3368.0
32	3664.0
33	3960.0
34	4339.5
35	4719.0
36	5015.0
37	5311.0
38	6021.5
39	6732.0
40	7524.5
41	8317.0
42	9840.0
43	11363.0
44	12759.5
45	14156.0
46	17197.0
47	20238.0
48	23386.5
49	26535.0
50	25843.5
51	25152.0
52	21336.0
53	17520.0
54	13826.5
55	10133.0
56	8222.5
57	6312.0
58	5493.5
59	4675.0
60	4106.5
61	3538.0
62	2976.0
63	2414.0
64	2170.0
65	1926.0
66	1651.5
67	1377.0
68	1147.5
69	918.0
70	825.0
71	732.0
72	621.0
73	510.0
74	466.5
75	390.5
76	358.0
77	290.0
78	222.0
79	141.0
80	60.0
81	53.0
82	46.0
83	35.0
84	24.0
85	13.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.20396242534228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91909016199513	35.14989082258959
2	12.27764029243259	11.83661822656578
3	5.106593801100187	7.384741671291578
4	2.8553084387324783	5.505487227736925
5	1.7417158381804156	4.197880240963616
6	1.2843397171013635	3.7146158078717315
7	0.8706840319779514	2.937929425326748
8	0.5929005264169478	2.286412375789457
9	0.44959995561375404	1.9505249430156864
>10	1.7954105886973628	13.974514975513788
>50	0.07806125159626016	2.579703624791929
>100	0.023714810611522072	1.9709774001826077
>500	0.002964351326440259	0.9168998297127887
>1k	9.881171088134196E-4	0.6072972898097692
>5k	0.0	0.0
>10k+	9.881171088134196E-4	4.986506138838019
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10469	4.341263357813154	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	1275	0.5287143739814473	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	699	0.28985988032394644	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	678	0.2811516435760167	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	548	0.22724351132692797	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	431	0.17872619230274805	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	396	0.16421246438953188	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.10615755273666706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.146779403776057E-4	0.0	0.0	0.30810570970056106	0.0
2	4.146779403776057E-4	0.0	0.0	0.9317813320284801	0.0
3	4.146779403776057E-4	0.0	0.0	1.341068459181177	0.0
4	4.146779403776057E-4	0.0	0.0	1.9775990976608018	0.0
5	4.146779403776057E-4	0.0	0.0	3.4546819212858333	0.0
6	4.146779403776057E-4	0.0	0.0	5.018017756509407	0.0
7	4.146779403776057E-4	0.0	0.0	6.315130354010558	0.0
8	4.146779403776057E-4	0.0	0.0	8.216843388582257	0.0
9	8.293558807552114E-4	0.0	0.0	9.197971395515673	0.0
10	8.293558807552114E-4	0.0	0.0	11.188840187268557	0.0
11	8.293558807552114E-4	0.0	0.0	15.86101654150304	0.0
12	8.293558807552114E-4	0.0	0.0	19.510182416825973	0.0
13	8.293558807552114E-4	0.0	0.0	20.927966294977008	0.0
14	8.293558807552114E-4	0.0	0.0	21.44175226310486	0.0
15	8.293558807552114E-4	0.0	0.0	22.14504604998528	0.0
16	8.293558807552114E-4	0.0	0.0	23.716675444016406	0.0
17	8.293558807552114E-4	0.0	0.0	25.717496506338353	0.0
18	8.293558807552114E-4	0.0	0.0	27.56073995131681	0.0
19	8.293558807552114E-4	0.0	0.0	28.598678836081955	0.0
20	8.293558807552114E-4	0.0	0.0	29.49355383141683	0.0
21	8.293558807552114E-4	0.0	0.0	30.56052017200841	0.0
22	8.293558807552114E-4	0.0	0.0	31.477373098183296	0.0
23	8.293558807552114E-4	0.0	0.0	32.228769526147516	0.0
24	8.293558807552114E-4	0.0	0.0	32.67662170175533	0.0
25	8.293558807552114E-4	0.0	0.0	33.038635543704984	0.0
26	8.293558807552114E-4	0.0	0.0	33.328910101969306	0.0
27	0.0012440338211328172	0.0	0.0	33.635357099908354	0.0
28	0.0012440338211328172	0.0	0.0	33.89079871118096	0.0
29	0.0012440338211328172	0.0	0.0	34.165315507710936	0.0
30	0.0012440338211328172	0.0	0.0	34.41951308516241	0.0
31	0.0012440338211328172	0.0	0.0	34.66458774792557	0.0
32	0.0012440338211328172	0.0	0.0	34.97103474586462	0.0
33	0.0012440338211328172	0.0	0.0	35.23476991594478	0.0
34	0.0012440338211328172	0.0	0.0	35.45579325816605	0.0
35	0.0012440338211328172	0.0	0.0	35.71330825914054	0.0
36	0.0016587117615104229	0.0	0.0	35.93184353371954	0.0
37	0.0016587117615104229	0.0	0.0	36.17028334943666	0.0
38	0.0016587117615104229	0.0	0.0	36.421163503365115	0.0
39	0.0016587117615104229	0.0	0.0	36.810546089379685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGACC	30	2.1583455E-6	45.000004	5
ATTCGAG	30	2.1583455E-6	45.000004	14
TACGGGA	30	2.1583455E-6	45.000004	4
GCTTGCG	30	2.1583455E-6	45.000004	1
GCCCTAC	30	2.1583455E-6	45.000004	26
GCGATCG	30	2.1583455E-6	45.000004	9
CACAAAT	30	2.1583455E-6	45.000004	32
TTCGAGC	30	2.1583455E-6	45.000004	15
AGCAACG	25	3.8816113E-5	45.0	10
TCGCATT	25	3.8816113E-5	45.0	22
CACGGGA	40	6.7757355E-9	45.0	4
ACGGGAG	45	3.8198777E-10	45.0	5
ACGGGAA	20	7.022192E-4	45.0	5
TATTCGG	20	7.022192E-4	45.0	2
TTGCGGT	20	7.022192E-4	45.0	3
AGCTCGC	25	3.8816113E-5	45.0	19
TTGTTCG	25	3.8816113E-5	45.0	1
GGCGATA	25	3.8816113E-5	45.0	8
GCTCGCA	25	3.8816113E-5	45.0	20
CGATCTG	25	3.8816113E-5	45.0	10
>>END_MODULE