##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49801
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094556334210154	33.0	31.0	34.0	30.0	34.0
2	32.279391980080725	34.0	31.0	34.0	30.0	34.0
3	32.37248248027148	34.0	31.0	34.0	30.0	34.0
4	35.92779261460613	37.0	35.0	37.0	35.0	37.0
5	35.92568422320837	37.0	35.0	37.0	35.0	37.0
6	35.89188972108994	37.0	35.0	37.0	35.0	37.0
7	35.935704102327264	37.0	35.0	37.0	35.0	37.0
8	35.72368024738459	37.0	35.0	37.0	35.0	37.0
9	37.58053051143551	39.0	37.0	39.0	35.0	39.0
10	37.08027951245959	39.0	37.0	39.0	33.0	39.0
11	37.06479789562459	39.0	37.0	39.0	33.0	39.0
12	37.08064095098492	39.0	37.0	39.0	33.0	39.0
13	37.03903536073573	39.0	37.0	39.0	33.0	39.0
14	38.19104034055541	40.0	37.0	41.0	33.0	41.0
15	38.22200357422542	40.0	37.0	41.0	33.0	41.0
16	38.250717857071145	40.0	38.0	41.0	33.0	41.0
17	38.208831147968915	40.0	37.0	41.0	33.0	41.0
18	37.95510130318668	39.0	37.0	40.0	33.0	41.0
19	37.65860123290697	39.0	37.0	40.0	34.0	41.0
20	37.24758538985161	39.0	35.0	40.0	33.0	41.0
21	37.0913636272364	38.0	35.0	40.0	33.0	41.0
22	37.09794984036465	38.0	35.0	40.0	33.0	41.0
23	37.00391558402442	38.0	35.0	40.0	33.0	41.0
24	36.855906507901445	38.0	35.0	40.0	33.0	41.0
25	36.80644966968535	38.0	35.0	40.0	33.0	41.0
26	36.71104997891609	38.0	35.0	40.0	32.0	41.0
27	36.57836188028353	38.0	35.0	40.0	32.0	41.0
28	36.47876548663682	38.0	35.0	40.0	32.0	41.0
29	36.37093632657979	38.0	35.0	40.0	31.0	41.0
30	36.151201783096724	38.0	35.0	40.0	31.0	41.0
31	35.87259291982089	38.0	35.0	40.0	30.0	41.0
32	35.464187466115135	38.0	35.0	40.0	28.0	41.0
33	35.01154595289251	38.0	35.0	40.0	24.0	41.0
34	34.68866087026365	38.0	34.0	40.0	21.0	41.0
35	34.31095761129295	38.0	34.0	40.0	19.0	41.0
36	33.984859741772254	38.0	33.0	40.0	18.0	41.0
37	33.82924037669926	37.0	33.0	40.0	18.0	41.0
38	33.7315314953515	37.0	33.0	40.0	17.0	41.0
39	33.66755687636795	37.0	33.0	40.0	15.0	41.0
40	33.531475271580895	37.0	33.0	40.0	15.0	41.0
41	33.29941165840043	37.0	33.0	40.0	15.0	41.0
42	33.09098210879299	37.0	33.0	40.0	15.0	41.0
43	32.97692817413305	37.0	32.0	40.0	13.0	41.0
44	32.89986144856529	37.0	32.0	40.0	12.0	41.0
45	32.88901829280537	37.0	32.0	40.0	12.0	41.0
46	32.61261822051766	36.0	31.0	40.0	10.0	41.0
47	32.353306158510875	36.0	31.0	40.0	10.0	41.0
48	32.258267906266944	36.0	31.0	40.0	10.0	41.0
49	32.268187385795464	36.0	31.0	40.0	10.0	41.0
50	32.16979578723319	35.0	31.0	40.0	10.0	41.0
51	31.046324370996565	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	1.0
12	4.0
13	2.0
14	3.0
15	6.0
16	9.0
17	14.0
18	19.0
19	46.0
20	73.0
21	91.0
22	187.0
23	273.0
24	472.0
25	862.0
26	1219.0
27	1510.0
28	1444.0
29	1336.0
30	1386.0
31	1479.0
32	1619.0
33	2102.0
34	3077.0
35	4168.0
36	4935.0
37	6006.0
38	9408.0
39	8047.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.20033734262364	4.754924599907633	39.708037991204996	32.33670006626373
2	36.06754884440072	4.8352442722033695	41.236119756631396	17.86108712676452
3	18.682355775988434	5.042067428364892	57.13941487118732	19.13616192445935
4	15.264753719804824	5.375394068392201	57.95465954498905	21.405192666813917
5	13.214594084456136	6.668540792353567	58.08517901246964	22.03168611072067
6	17.57394429830726	7.546033212184494	61.53290094576415	13.3471215437441
7	65.4565169374109	3.007971727475352	26.911106202686693	4.62440513242706
8	64.40432923033674	3.5420975482420034	26.117949438766292	5.935623782654967
9	59.731732294532236	5.521977470331921	28.342804361358205	6.403485873777635
10	28.947209895383626	21.861006807092227	34.246300275094875	14.945483022429269
11	20.66223569807835	18.94941868637176	39.45904700708821	20.92929860846168
12	19.602016023774624	15.69044798297223	44.92078472319833	19.78675127005482
13	20.106021967430372	16.93741089536355	46.752073251541134	16.20449388566495
14	18.021726471355997	18.987570530712233	45.36455091263228	17.62615208529949
15	14.14831027489408	18.33898917692416	47.585389851609406	19.92731069657236
16	14.616172366016746	17.51772052770025	46.035220176301685	21.830886929981325
17	13.710568060882311	17.493624626011524	44.58143410774884	24.214373205357322
18	15.174394088472118	17.399249011064036	45.67779763458565	21.748559265878196
19	16.182405975783617	20.065862131282504	45.24005542057388	18.51167647235999
20	17.387201060219674	22.12806971747555	43.12363205558121	17.36109716672356
21	16.4936447059296	22.96339430935122	42.950944760145376	17.592016224573804
22	14.634244292283288	21.204393486074576	41.37667918314894	22.784683038493203
23	14.73062789903817	22.124053733860766	40.17991606594245	22.965402301158612
24	15.584024417180379	21.549768076946247	40.54336258308066	22.322844922792715
25	15.519768679343787	22.686291439930926	38.1498363486677	23.64410353205759
26	15.228609867271741	23.437280375896066	39.469086966125175	21.865022790707016
27	15.935422983474226	22.34493283267404	42.09754824200317	19.622095941848556
28	15.654304130439147	20.650187747233993	41.824461356197666	21.871046766129194
29	17.077970321881086	19.614063974618983	40.39477118933355	22.91319451416638
30	21.178289592578462	20.604003935663943	39.49719885142869	18.72050762032891
31	21.262625248488987	24.316780787534388	34.73625027609887	19.684343687877753
32	19.443384670990543	24.47742013212586	33.94309351217847	22.136101684705125
33	20.887130780506418	22.714403325234432	33.05756912511797	23.34089676914118
34	22.51762012810988	20.973474428224335	33.34471195357523	23.16419349009056
35	22.6943234071605	21.3810967651252	32.97925744462963	22.945322383084676
36	23.712375253508966	24.674203329250417	33.91698961868236	17.69643179855826
37	23.921206401477882	23.642095540250196	34.103732856769945	18.332965201501978
38	21.732495331419045	24.706331198168712	31.031505391458005	22.529668078954238
39	21.62807975743459	25.505511937511294	29.85683018413285	23.00957812092127
40	24.87500250998976	23.555751892532278	32.617818919298806	18.951426678179153
41	22.124053733860766	23.66016746651674	34.62179474307745	19.59398405654505
42	21.75659123310777	25.262544928816688	34.44107548041204	18.5397883576635
43	20.45541254191683	23.74249513061987	35.36675970362041	20.435332623842896
44	21.18029758438586	22.222445332423042	32.91098572317825	23.68627136001285
45	23.531655990843557	20.01566233609767	31.744342483082672	24.708339189976105
46	24.20232525451296	21.51161623260577	35.061544948896604	19.224513563984658
47	19.674303728840787	24.87500250998976	36.74625007529969	18.704443685869762
48	18.39320495572378	24.061765827995423	36.97315314953515	20.57187606674565
49	21.439328527539608	21.624063773819802	36.54344290275296	20.393164795887632
50	21.28270516656292	18.83897913696512	35.017369129133954	24.860946567338004
51	18.792795325395073	18.941386719142187	33.10576092849541	29.16005702696733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	285.0
2	431.0
3	1911.5
4	3392.0
5	2159.0
6	926.0
7	881.0
8	836.0
9	788.5
10	741.0
11	732.0
12	723.0
13	638.5
14	554.0
15	535.0
16	516.0
17	484.0
18	452.0
19	449.0
20	446.0
21	396.0
22	346.0
23	323.5
24	301.0
25	285.5
26	306.0
27	342.0
28	330.0
29	318.0
30	386.0
31	454.0
32	469.0
33	484.0
34	592.0
35	700.0
36	772.5
37	845.0
38	944.0
39	1043.0
40	1185.0
41	1327.0
42	1601.5
43	1876.0
44	2177.5
45	2479.0
46	3116.5
47	3754.0
48	4624.5
49	5495.0
50	5593.5
51	5692.0
52	4883.5
53	4075.0
54	3226.0
55	2377.0
56	2019.0
57	1661.0
58	1590.0
59	1519.0
60	1447.0
61	1375.0
62	1248.5
63	1122.0
64	997.5
65	873.0
66	731.5
67	590.0
68	499.0
69	408.0
70	355.5
71	303.0
72	255.5
73	208.0
74	184.0
75	127.5
76	95.0
77	87.0
78	79.0
79	67.0
80	55.0
81	34.0
82	13.0
83	7.5
84	2.0
85	3.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	49801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.82994317383185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82215998599685	56.31814622196342
2	11.360056012602836	15.63824019598
3	3.617480599801622	7.469729523503545
4	1.391563101697882	3.8312483685066567
5	0.5805472898068732	1.9979518483564587
6	0.3413267985296692	1.409610248790185
7	0.218799229826711	1.0541956988815486
8	0.14586615321780733	0.8031967229573703
9	0.06709843048019137	0.4156543041304392
>10	0.4230118443316413	4.696692837493223
>50	0.020421261450493028	1.1023875022589908
>100	0.008751969193068442	1.178691190939941
>500	0.0	0.0
>1k	0.0029173230643561466	4.084255336238228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2034	4.084255336238228	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	213	0.42770225497479974	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	210	0.4216782795526194	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	164	0.32931065641252183	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	99	0.19879118893194914	TruSeq Adapter, Index 15 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.1927672135097689	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	94	0.18875122989498203	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	73	0.14658340193972008	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.14457541013232666	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.1265034838657858	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGT	52	0.10441557398445814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0020079918073934258	0.0	0.0	0.3634465171382101	0.0
2	0.0020079918073934258	0.0	0.0	1.2931467239613663	0.0
3	0.0020079918073934258	0.0	0.0	1.7630168068914278	0.0
4	0.0020079918073934258	0.0	0.0	2.546133611774864	0.0
5	0.0020079918073934258	0.0	0.0	4.246902672637096	0.0
6	0.0020079918073934258	0.0	0.0	5.5601293146723965	0.0
7	0.0020079918073934258	0.0	0.0	6.835204112367221	0.0
8	0.0020079918073934258	0.0	0.0	8.752836288427943	0.0
9	0.0020079918073934258	0.0	0.0	9.658440593562378	0.0
10	0.0020079918073934258	0.0	0.0	11.70458424529628	0.0
11	0.0020079918073934258	0.0	0.0	16.30087749241983	0.0
12	0.0020079918073934258	0.0	0.0	20.511636312523844	0.0
13	0.0020079918073934258	0.0	0.0	21.8690387743218	0.0
14	0.0020079918073934258	0.0	0.0	22.318828939177926	0.0
15	0.0020079918073934258	0.0	0.0	22.973434268388186	0.0
16	0.0020079918073934258	0.0	0.0	24.579827714302926	0.0
17	0.0020079918073934258	0.0	0.0	26.45930804602317	0.0
18	0.0020079918073934258	0.0	0.0	28.342804361358205	0.0
19	0.0020079918073934258	0.0	0.0	29.445191863617197	0.0
20	0.0020079918073934258	0.0	0.0	30.43713981646955	0.0
21	0.0020079918073934258	0.0	0.0	31.589727113913376	0.0
22	0.0020079918073934258	0.0	0.0	32.533483263388284	0.0
23	0.0020079918073934258	0.0	0.0	33.260376297664706	0.0
24	0.0020079918073934258	0.0	0.0	33.64791871649164	0.0
25	0.0020079918073934258	0.0	0.0	33.92502158591193	0.0
26	0.0020079918073934258	0.0	0.0	34.167988594606534	0.0
27	0.0020079918073934258	0.0	0.0	34.38284371799763	0.0
28	0.0020079918073934258	0.0	0.0	34.58163490692958	0.0
29	0.0020079918073934258	0.0	0.0	34.74629023513584	0.0
30	0.0020079918073934258	0.0	0.0	34.939057448645606	0.0
31	0.0020079918073934258	0.0	0.0	35.115760727696234	0.0
32	0.0020079918073934258	0.0	0.0	35.26836810505813	0.0
33	0.0020079918073934258	0.0	0.0	35.45109535953093	0.0
34	0.0020079918073934258	0.0	0.0	35.569566876167144	0.0
35	0.0020079918073934258	0.0	0.0	35.70008634364772	0.0
36	0.0020079918073934258	0.0	0.0	35.84265376197265	0.0
37	0.0020079918073934258	0.0	0.0	35.949077327764506	0.0
38	0.0020079918073934258	0.0	0.0	36.04746892632678	0.0
39	0.0020079918073934258	0.0	0.0	36.212124254533045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGCT	20	6.968403E-4	45.000004	5
ATGGGAC	20	6.968403E-4	45.000004	5
TCCCAAG	20	6.968403E-4	45.000004	16
GGACCGA	20	6.968403E-4	45.000004	8
ATTGGGA	20	6.968403E-4	45.000004	4
CAACATG	20	6.968403E-4	45.000004	30
ACTCCGG	20	6.968403E-4	45.000004	30
AACCCGA	20	6.968403E-4	45.000004	24
GCAACCC	20	6.968403E-4	45.000004	22
GTGGGAC	20	6.968403E-4	45.000004	5
CGGGATG	20	6.968403E-4	45.000004	6
GCATGGG	20	6.968403E-4	45.000004	3
TGGGTAT	20	6.968403E-4	45.000004	6
GTGCGGG	20	6.968403E-4	45.000004	3
CGGTCGG	20	6.968403E-4	45.000004	1
TGGGAAG	20	6.968403E-4	45.000004	6
CCGACTC	20	6.968403E-4	45.000004	27
GACTCCG	20	6.968403E-4	45.000004	29
TTCGGGC	20	6.968403E-4	45.000004	4
GCCCTTG	20	6.968403E-4	45.000004	1
>>END_MODULE