FastQCFastQC Report
Sat 14 Jan 2017
SRR2933592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60634
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50248.285780255302305No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC3860.6366065243922552No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT2600.4288023221294983No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC2240.3694296929115678No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC2050.3380941386021044No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC1820.30016162549064884No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC1580.26057987267869515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.22594583896823564No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.17646864795329353No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA950.15667777154731669No Hit
TTTTTTGGGGTTTTAAGGCTGTCTCTTATACACATCTGACGCGTCGTAAGT760.12534221723785335No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG680.11214829963386878No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA660.10884982023287265No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG630.10390210113137843No Hit
TTTAATCCCCTGTCCTAGGCCTGTCTCTTATACACATCTGACGCGTCGTAA620.10225286143088037No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA620.10225286143088037No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC206.980461E-445.05
GTGTTGG253.8470866E-545.02
GTTCTTG206.980461E-445.01
TCTTGCG206.980461E-445.01
TCGTTGG206.980461E-445.02
GCATGGG253.8470866E-545.03
GTGATAT253.8470866E-545.01
CTTGCGG206.980461E-445.02
TGGGCGA253.8470866E-545.06
CTTCGGG253.8470866E-545.03
GCTCCTG253.8470866E-545.01
CGTTGGG253.8470866E-545.03
TGGGACC453.7289283E-1045.06
TACTTGG206.980461E-445.02
TATGGGC206.980461E-445.04
TCGGGCT206.980461E-445.05
CATGGGT302.13153E-644.9999964
CGTTTTT18350.044.5095371
TTGGGAT1150.041.086965
TCTGGGA555.820766E-1140.9090924
Produced by FastQC (version 0.11.5)