##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933592.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60634
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08221459906983	33.0	31.0	34.0	30.0	34.0
2	32.310386911633735	34.0	31.0	34.0	30.0	34.0
3	32.331958966916254	33.0	31.0	34.0	30.0	34.0
4	35.949351848797704	37.0	35.0	37.0	35.0	37.0
5	35.983095293069894	37.0	35.0	37.0	35.0	37.0
6	35.974354322657256	37.0	35.0	37.0	35.0	37.0
7	36.02299040142494	37.0	35.0	37.0	35.0	37.0
8	35.856400699277636	37.0	35.0	37.0	35.0	37.0
9	37.68413761256061	39.0	38.0	39.0	35.0	39.0
10	37.2387439390441	39.0	37.0	39.0	34.0	39.0
11	37.24466470956889	39.0	37.0	39.0	34.0	39.0
12	37.30834185440512	39.0	37.0	39.0	34.0	39.0
13	37.21745225451067	39.0	37.0	39.0	34.0	39.0
14	38.407708546360126	40.0	38.0	41.0	34.0	41.0
15	38.436603225912854	40.0	38.0	41.0	34.0	41.0
16	38.4996536596629	40.0	38.0	41.0	34.0	41.0
17	38.488240920935446	40.0	38.0	41.0	34.0	41.0
18	37.97757034007323	39.0	38.0	40.0	34.0	41.0
19	37.3960319292806	37.0	37.0	40.0	34.0	41.0
20	36.71108618926675	37.0	35.0	40.0	33.0	41.0
21	36.58454002704753	37.0	35.0	40.0	33.0	41.0
22	36.57263251640993	37.0	35.0	40.0	33.0	41.0
23	36.54169277962859	37.0	35.0	40.0	33.0	41.0
24	36.41188112280239	37.0	35.0	40.0	33.0	41.0
25	36.32330045848864	37.0	35.0	40.0	33.0	41.0
26	36.22271332915526	36.0	35.0	40.0	33.0	41.0
27	36.11176897450275	36.0	35.0	40.0	32.0	41.0
28	35.94414025134413	36.0	35.0	40.0	32.0	41.0
29	35.796483820958535	36.0	35.0	40.0	31.0	41.0
30	35.47991226044793	36.0	35.0	40.0	30.0	41.0
31	34.98354058778903	36.0	35.0	40.0	27.0	41.0
32	34.31152488702708	36.0	34.0	40.0	22.0	41.0
33	33.5489989115018	36.0	33.0	40.0	18.0	41.0
34	32.949153940033646	36.0	33.0	40.0	12.0	41.0
35	32.47390902793812	36.0	32.0	40.0	10.0	41.0
36	32.09898736682389	35.0	31.0	40.0	10.0	41.0
37	31.78813866807402	35.0	30.0	40.0	9.0	41.0
38	31.70533034271201	35.0	30.0	40.0	8.0	41.0
39	31.59257182438896	35.0	30.0	40.0	8.0	41.0
40	31.355938912161495	35.0	29.0	40.0	8.0	41.0
41	31.116502292443183	35.0	28.0	40.0	8.0	41.0
42	30.934426229508198	35.0	27.0	40.0	7.0	41.0
43	30.689959428703368	35.0	26.0	39.0	7.0	41.0
44	30.511346769139426	35.0	25.0	39.0	7.0	41.0
45	30.581142593264506	35.0	25.0	39.0	7.0	41.0
46	30.336725269650692	35.0	24.0	39.0	7.0	41.0
47	30.129514793680112	35.0	23.0	39.0	7.0	41.0
48	29.980374047564073	35.0	23.0	39.0	7.0	41.0
49	30.062753570603952	35.0	24.0	39.0	7.0	41.0
50	29.96863146089653	35.0	24.0	39.0	7.0	41.0
51	28.997212784906157	35.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	3.0
15	7.0
16	9.0
17	17.0
18	32.0
19	55.0
20	125.0
21	192.0
22	281.0
23	512.0
24	907.0
25	1673.0
26	2417.0
27	2647.0
28	2384.0
29	2057.0
30	1899.0
31	1948.0
32	2055.0
33	2414.0
34	3431.0
35	5303.0
36	6560.0
37	6920.0
38	9939.0
39	6839.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.679288847841143	4.932875944189728	34.40149091268925	41.98634429527988
2	47.13362140053435	4.325955734406438	33.82920473661642	14.711218128442788
3	16.03390836824224	5.112643071544018	63.61117524821057	15.242273312003165
4	12.710690371738629	4.9592637793976975	63.74971138305241	18.58033446581126
5	10.251673978296004	5.650295213906389	65.6331431210212	18.464887686776397
6	14.061417686446548	6.059306659629911	68.89369000890589	10.985585645017647
7	53.51617904146189	2.6074479664874493	39.39538872579741	4.480984266253257
8	52.16215324735297	3.03460104891645	39.09357786060626	5.70966784312432
9	47.85598838935251	5.1555233037569685	41.09080713790942	5.8976811689811
10	23.10254972457697	17.620476960121383	45.86040835174984	13.416564963551803
11	15.525942540488835	15.895372233400401	50.45519015733747	18.123495068773295
12	14.70956888874229	12.700794933535638	55.56783322888148	17.021802948840584
13	17.058086222251543	14.490220008576046	56.56067552858133	11.891018240591087
14	14.354982353135204	16.779364712867366	54.853712438565815	14.011940495431608
15	10.396807071939836	15.201042319490716	57.50568987696671	16.89646073160273
16	10.10159316555068	15.017976712735429	54.83722004156084	20.04321008015305
17	10.860243427779794	14.757396840056733	52.9076095919781	21.474750140185375
18	11.450671240558103	13.720025068443448	56.09558993304087	18.73371375795758
19	11.851436487779134	16.04215456674473	56.615100438697766	15.491308506778376
20	12.62492990731273	18.684236566942637	53.96642147969786	14.724412046046773
21	11.869578124484613	19.24003034601049	54.16597948345812	14.724412046046773
22	9.852557970775473	18.01134676913943	52.18194412375895	19.954151136326153
23	10.124682521357654	19.10974040967114	50.22759507866873	20.53798199030247
24	11.20493452518389	18.17956921859023	50.69597915361018	19.919517102615693
25	10.678827060725006	20.114127387274465	48.675660520500045	20.53138503150048
26	10.492462974568724	20.321931589537222	50.87409704126398	18.311508394630074
27	11.406141768644655	19.65069103143451	52.08134050202856	16.861826697892273
28	10.619454431507075	16.69525348814197	52.09453441963255	20.59075766071841
29	13.734868225747931	15.29175050301811	51.09674440083122	19.876636870402745
30	16.52868027839166	17.51327637958901	49.61902562918495	16.339017712834384
31	15.895372233400401	22.42141372827127	43.73453837780783	17.9486756605205
32	15.680971072335653	22.49562951479368	42.48606392453079	19.337335488339875
33	17.152092885179933	19.185605435894054	42.42998977471385	21.23231190421216
34	17.841475079988125	19.517102615694164	40.54161031764357	22.09981198667414
35	20.815054259986145	20.140515222482435	38.61529834746182	20.429132170069597
36	20.735890754362238	20.97503051093446	41.55424349374938	16.73483524095392
37	17.909093907708545	22.07672263086717	40.498730085430616	19.515453375993665
38	20.325230068938218	21.398885113962464	37.195302965332985	21.080581851766336
39	22.18722169080054	19.987135930336112	40.088069400006596	17.737572978856747
40	19.437939110070257	23.68308209915229	40.41956657980671	16.459412210970743
41	18.11195039086981	27.921628129432328	36.46139129861134	17.50503018108652
42	22.06847643236468	23.805125836989145	36.69228485668107	17.434112873965102
43	22.622620971732033	18.844212817890952	40.22990401424943	18.303262196127584
44	19.45443150707524	18.10865191146881	39.70874426889204	22.728172312563906
45	21.807896559685986	17.88930303130257	38.40749414519906	21.895306263812383
46	21.252102780618134	20.34996866444569	41.560840452551375	16.8370881023848
47	16.215324735297028	24.202592604809183	42.43163901441436	17.150443645479434
48	17.44565755186859	21.822739716990466	42.67242801068708	18.05917472045387
49	20.92225484051852	17.072929379556022	44.057789359105456	17.947026420820002
50	19.089949533265166	16.19553385889105	41.11884421281789	23.595672395025893
51	16.69525348814197	16.96737803872415	38.435531220107535	27.901837253026358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	206.0
1	330.0
2	454.0
3	4037.0
4	7620.0
5	4661.0
6	1702.0
7	1588.0
8	1474.0
9	1449.0
10	1424.0
11	1389.0
12	1354.0
13	1270.5
14	1187.0
15	1099.5
16	1012.0
17	925.0
18	838.0
19	750.0
20	662.0
21	591.5
22	521.0
23	454.0
24	387.0
25	385.0
26	396.5
27	410.0
28	421.5
29	433.0
30	465.5
31	498.0
32	501.0
33	504.0
34	553.0
35	602.0
36	678.0
37	754.0
38	797.0
39	840.0
40	1148.5
41	1457.0
42	1892.0
43	2327.0
44	2720.5
45	3114.0
46	3953.5
47	4793.0
48	5797.5
49	6802.0
50	6707.0
51	6612.0
52	5448.0
53	4284.0
54	3296.0
55	2308.0
56	1851.0
57	1394.0
58	1219.5
59	1045.0
60	911.0
61	777.0
62	731.0
63	685.0
64	620.0
65	555.0
66	469.0
67	383.0
68	317.5
69	252.0
70	219.5
71	187.0
72	178.5
73	170.0
74	132.5
75	76.5
76	58.0
77	45.0
78	32.0
79	23.0
80	14.0
81	13.0
82	12.0
83	9.5
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	2.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	60634.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.23132236039186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49055943114175	49.89774713856912
2	10.270153796428476	12.577101955998286
3	3.88396584695774	7.134610944354653
4	1.6672502491448273	4.083517498433222
5	0.8834540900153527	2.7047531088168353
6	0.44442050259918653	1.6327473034930897
7	0.2881999622915937	1.2352805356730547
8	0.19123548899722573	0.936768149882904
9	0.18315511622269506	1.0093346967048191
>10	0.6383494491879226	6.4056469967345055
>50	0.03501494868963288	1.360622752910908
>100	0.021547660732081773	2.736088663126299
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0026934575915102217	8.285780255302305
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5024	8.285780255302305	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	386	0.6366065243922552	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	260	0.4288023221294983	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	224	0.3694296929115678	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	205	0.3380941386021044	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	182	0.30016162549064884	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	158	0.26057987267869515	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.22594583896823564	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.17646864795329353	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	95	0.15667777154731669	No Hit
TTTTTTGGGGTTTTAAGGCTGTCTCTTATACACATCTGACGCGTCGTAAGT	76	0.12534221723785335	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	68	0.11214829963386878	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	66	0.10884982023287265	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	63	0.10390210113137843	No Hit
TTTAATCCCCTGTCCTAGGCCTGTCTCTTATACACATCTGACGCGTCGTAA	62	0.10225286143088037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	62	0.10225286143088037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45354091763696935	0.0
2	0.0	0.0	0.0	1.5156512847577266	0.0
3	0.0	0.0	0.0	2.1852426031599435	0.0
4	0.0	0.0	0.0	3.0857274796318896	0.0
5	0.0	0.0	0.0	4.957614539697199	0.0
6	0.0	0.0	0.0	6.362766764521556	0.0
7	0.0	0.0	0.0	7.711844839528977	0.0
8	0.0	0.0	0.0	9.689283240426164	0.0
9	0.0	0.0	0.0	10.517201570076194	0.0
10	0.0	0.0	0.0	12.684302536530659	0.0
11	0.0	0.0	0.0	17.90414618860705	0.0
12	0.0	0.0	0.0	22.635814889336014	0.0
13	0.0	0.0	0.0	24.09539202427681	0.0
14	0.0	0.0	0.0	24.63304416663918	0.0
15	0.0	0.0	0.0	25.355411155457332	0.0
16	0.0	0.0	0.0	27.318006399050038	0.0
17	0.0	0.0	0.0	29.643434376752317	0.0
18	0.0	0.0	0.0	31.89794504733318	0.0
19	0.0	0.0	0.0	33.23217996503612	0.0
20	0.0	0.0	0.0	34.23821618233994	0.0
21	0.0	0.0	0.0	35.71758419368671	0.0
22	0.0	0.0	0.0	36.804433156314936	0.0
23	0.0	0.0	0.0	37.55153874064057	0.0
24	0.0	0.0	0.0	38.02157205528251	0.0
25	0.0	0.0	0.0	38.303592044067685	0.0
26	0.0	0.0	0.0	38.52953788303592	0.0
27	0.0	0.0	0.0	38.7175512088927	0.0
28	0.0	0.0	0.0	38.890721377445	0.0
29	0.0	0.0	0.0	39.11171949731174	0.0
30	0.0	0.0	0.0	39.28324042616354	0.0
31	0.0	0.0	0.0	39.44486591681235	0.0
32	0.0	0.0	0.0	39.59164825015668	0.0
33	0.0	0.0	0.0	39.731833624699014	0.0
34	0.0	0.0	0.0	39.87036975954085	0.0
35	0.0	0.0	0.0	39.989115017976715	0.0
36	0.0	0.0	0.0	40.096315598509086	0.0
37	0.0	0.0	0.0	40.203516179041465	0.0
38	0.0	0.0	0.0	40.337104594781806	0.0
39	0.0	0.0	0.0	40.46244681201966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGC	20	6.980461E-4	45.0	5
GTGTTGG	25	3.8470866E-5	45.0	2
GTTCTTG	20	6.980461E-4	45.0	1
TCTTGCG	20	6.980461E-4	45.0	1
TCGTTGG	20	6.980461E-4	45.0	2
GCATGGG	25	3.8470866E-5	45.0	3
GTGATAT	25	3.8470866E-5	45.0	1
CTTGCGG	20	6.980461E-4	45.0	2
TGGGCGA	25	3.8470866E-5	45.0	6
CTTCGGG	25	3.8470866E-5	45.0	3
GCTCCTG	25	3.8470866E-5	45.0	1
CGTTGGG	25	3.8470866E-5	45.0	3
TGGGACC	45	3.7289283E-10	45.0	6
TACTTGG	20	6.980461E-4	45.0	2
TATGGGC	20	6.980461E-4	45.0	4
TCGGGCT	20	6.980461E-4	45.0	5
CATGGGT	30	2.13153E-6	44.999996	4
CGTTTTT	1835	0.0	44.509537	1
TTGGGAT	115	0.0	41.08696	5
TCTGGGA	55	5.820766E-11	40.909092	4
>>END_MODULE