##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933591.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61204
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.023152081563296	33.0	31.0	34.0	30.0	34.0
2	32.24223906934187	34.0	31.0	34.0	30.0	34.0
3	32.29319979086334	33.0	31.0	34.0	30.0	34.0
4	35.87231226717208	37.0	35.0	37.0	35.0	37.0
5	35.97019802627279	37.0	35.0	37.0	35.0	37.0
6	35.972648846480624	37.0	35.0	37.0	35.0	37.0
7	35.94493823933076	37.0	35.0	37.0	35.0	37.0
8	35.819946408731454	37.0	35.0	37.0	35.0	37.0
9	37.629926148617734	39.0	38.0	39.0	35.0	39.0
10	37.273233775570226	39.0	37.0	39.0	34.0	39.0
11	37.26944317364878	39.0	37.0	39.0	34.0	39.0
12	37.28865760407817	39.0	37.0	39.0	34.0	39.0
13	37.21029671263316	39.0	37.0	39.0	34.0	39.0
14	38.42528266126397	40.0	38.0	41.0	34.0	41.0
15	38.44580419580419	40.0	38.0	41.0	34.0	41.0
16	38.5531827985099	40.0	38.0	41.0	34.0	41.0
17	38.58970001960656	40.0	38.0	41.0	34.0	41.0
18	38.13621658715117	39.0	38.0	40.0	34.0	41.0
19	37.616430298673286	38.0	37.0	40.0	34.0	41.0
20	36.91051238481145	38.0	35.0	40.0	34.0	41.0
21	36.80730017645905	37.0	35.0	40.0	33.0	41.0
22	36.777808639958174	37.0	35.0	40.0	33.0	41.0
23	36.65778380498006	37.0	35.0	40.0	33.0	41.0
24	36.53027579896739	37.0	35.0	40.0	33.0	41.0
25	36.484249395464346	37.0	35.0	40.0	33.0	41.0
26	36.44755244755245	37.0	35.0	40.0	33.0	41.0
27	36.35398013201752	37.0	35.0	40.0	33.0	41.0
28	36.25320240507156	37.0	35.0	40.0	33.0	41.0
29	36.00230377099536	37.0	35.0	40.0	32.0	41.0
30	35.5897653748121	36.0	35.0	40.0	30.0	41.0
31	35.14015423828508	36.0	35.0	40.0	29.0	41.0
32	34.574325207502774	36.0	35.0	40.0	23.0	41.0
33	33.76758055029083	36.0	34.0	40.0	18.0	41.0
34	33.16368211228024	37.0	33.0	40.0	15.0	41.0
35	32.75936213319391	36.0	33.0	40.0	10.0	41.0
36	32.37843931769165	36.0	31.0	40.0	10.0	41.0
37	32.12667472714202	36.0	31.0	40.0	10.0	41.0
38	32.005930984902946	36.0	31.0	40.0	10.0	41.0
39	31.954006274099733	36.0	31.0	40.0	9.0	41.0
40	31.747565518593557	35.0	30.0	40.0	8.0	41.0
41	31.58911182275668	35.0	30.0	40.0	8.0	41.0
42	31.336987778576564	35.0	29.0	40.0	8.0	41.0
43	31.11196980589504	35.0	28.0	40.0	8.0	41.0
44	30.920479053656624	35.0	27.0	40.0	7.0	41.0
45	30.90436899549049	35.0	26.0	40.0	7.0	41.0
46	30.654744787922358	35.0	26.0	39.0	7.0	41.0
47	30.582265864976144	35.0	26.0	39.0	7.0	41.0
48	30.432406378668063	35.0	25.0	39.0	7.0	41.0
49	30.424122606365597	35.0	26.0	39.0	7.0	41.0
50	30.22539376511339	35.0	24.0	39.0	7.0	41.0
51	29.421965884582708	35.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	4.0
13	1.0
14	5.0
15	5.0
16	10.0
17	21.0
18	32.0
19	68.0
20	96.0
21	162.0
22	287.0
23	468.0
24	823.0
25	1571.0
26	2414.0
27	2587.0
28	2321.0
29	2058.0
30	1861.0
31	1907.0
32	1983.0
33	2373.0
34	3319.0
35	5221.0
36	6328.0
37	6603.0
38	10404.0
39	8263.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.346774720606497	5.073197830207176	34.04842820730671	41.53159924187961
2	46.809032089405925	4.400039213123326	33.88667407358996	14.904254623880792
3	16.30285602248219	5.189203320044442	62.87824325207503	15.62969740539834
4	13.374942814195151	5.065028429514411	62.97954382066532	18.580484935625122
5	10.558133455329717	5.734919286321156	65.18364812757336	18.523299130775765
6	14.386314619959482	6.249591529965362	68.083785373505	11.28030847657016
7	53.8722959283707	2.76125743415463	38.65433631788772	4.712110319586955
8	52.98019737272074	2.8870662048232143	38.45173518070714	5.681001241748905
9	48.57525651918175	5.148356316580616	40.22286125089863	6.053525913338998
10	23.20109796745311	17.977583164499052	45.19966015293118	13.621658715116657
11	16.222795895693093	16.00548983726554	49.63237696882557	18.139337298215803
12	15.020260113718056	12.816155806810011	55.30684269002026	16.85674138945167
13	17.240703222011632	14.566041435200313	55.64015423828508	12.553101104502973
14	14.670609764067708	16.936801516240767	54.36409385007516	14.028494869616365
15	10.89307888373309	15.404221946278021	56.48650415005555	17.216195019933338
16	10.453565126462323	15.090516959675837	54.28239984314751	20.173518070714334
17	11.03849421606431	14.907522384157899	52.20083654663094	21.853146853146853
18	11.747598196196327	14.123259917652442	55.298673289327496	18.83046859682374
19	12.172407032220116	16.065943402391998	55.77249852950787	15.989151035880008
20	12.90111757401477	18.564146134239593	53.388667407358994	15.14606888438664
21	12.02372393961179	19.199725508136723	53.62558002744918	15.150970524802302
22	10.4682700477093	18.087053133782106	51.66328998104699	19.781386837461604
23	10.43069080452258	18.908894843474283	49.71243709561467	20.947977256388473
24	11.458401411672439	18.4595778053722	50.12417489053003	19.957845892425333
25	11.26396967518463	19.753610875106205	48.219070648977194	20.76334880073198
26	10.731324750016338	20.676753153388667	50.19769949676491	18.394222599830076
27	11.696947911901184	19.426834847395593	51.87569439905888	17.000522841644337
28	11.051565257172733	16.791386183909548	51.305470230703875	20.851578328213844
29	13.788314489249068	15.46304163126593	50.89373243578851	19.85491144369649
30	16.734200379060194	17.39428795503562	49.132409646428336	16.73910201947585
31	15.97444611463303	22.340043134435657	43.734069668649106	17.951441082282205
32	16.041435200313707	22.377622377622377	42.08221684857199	19.498725573491928
33	17.69492190052938	19.202993268413827	42.02339716358408	21.078687667472714
34	17.83706947258349	19.90882948826874	39.847068819031435	22.40703222011633
35	20.9920920201294	20.292791320828705	38.58734723220705	20.127769426834845
36	21.201228677864194	20.820534605581333	41.17214561139795	16.806091105156526
37	18.15404221946278	22.049212469773217	39.90425462388079	19.89249068688321
38	20.58688974576825	21.30252924645448	37.34559832690674	20.76498268087053
39	22.323704333050127	20.03790601921443	39.97614534997712	17.662244297758317
40	19.781386837461604	23.759884974838247	40.11992680216979	16.338801385530356
41	18.214495784589243	27.44428468727534	36.76066923730475	17.580550290830665
42	22.00836546630939	23.536043395856478	37.048232141690086	17.407358996144044
43	22.402130579700675	18.7830860728057	40.05457159662767	18.760211750865956
44	19.787922358015816	17.600156852493303	40.028429514410824	22.58349127508006
45	21.521469185020585	17.810927390366643	38.992549506568196	21.675053918044572
46	20.76334880073198	20.358146526370827	41.47277955689171	17.40572511600549
47	16.828965427096268	23.696163649434677	42.47761584210183	16.99725508136723
48	17.600156852493303	21.34501013005686	42.992288085746026	18.062544931703812
49	20.55257826285864	17.245604862427292	44.07228285732959	18.129534017384486
50	19.25200967257042	16.32899810469904	40.887850467289724	23.53114175544082
51	17.245604862427292	16.623096529638588	38.582445591791384	27.548853016142736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	320.5
2	463.0
3	4021.0
4	7579.0
5	4636.0
6	1693.0
7	1591.0
8	1489.0
9	1427.0
10	1365.0
11	1354.5
12	1344.0
13	1279.5
14	1215.0
15	1072.0
16	929.0
17	879.5
18	830.0
19	735.0
20	640.0
21	585.0
22	530.0
23	477.0
24	424.0
25	395.5
26	377.0
27	387.0
28	398.5
29	410.0
30	444.0
31	478.0
32	480.0
33	482.0
34	553.5
35	625.0
36	661.0
37	697.0
38	825.0
39	953.0
40	1221.5
41	1490.0
42	1940.5
43	2391.0
44	2754.0
45	3117.0
46	3910.0
47	4703.0
48	5756.5
49	6810.0
50	6730.0
51	6650.0
52	5522.5
53	4395.0
54	3413.0
55	2431.0
56	1936.0
57	1441.0
58	1265.0
59	1089.0
60	985.0
61	881.0
62	813.0
63	745.0
64	685.5
65	626.0
66	511.5
67	397.0
68	345.0
69	293.0
70	265.5
71	238.0
72	215.0
73	192.0
74	143.5
75	84.5
76	74.0
77	52.0
78	30.0
79	24.0
80	18.0
81	14.5
82	11.0
83	7.0
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	61204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.22802431213646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.2456636601377	49.74511469838573
2	10.44190638842931	12.786745964316056
3	3.8506697977264235	7.073067119796092
4	1.7372044617601536	4.254623880792105
5	0.8992901745210012	2.7530880334618653
6	0.44831082884133	1.64695117966146
7	0.33890163846933874	1.4525194431736488
8	0.19480172919891123	0.9541860009149729
9	0.1334258419170625	0.735246062348866
>10	0.6564551422319475	6.540422194627802
>50	0.029353685221753748	1.1339128161558067
>100	0.02134813470673	2.6910005881968497
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.00266851683834125	8.233122018168746
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5039	8.233122018168746	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	376	0.6143389320959415	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	261	0.4264427161623423	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	259	0.4231749558852363	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	182	0.29736618521665253	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	181	0.2957323050780995	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	148	0.24181426050584928	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125	0.20423501731912946	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115	0.18789621593359912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	83	0.13561205149990196	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	74	0.12090713025292464	No Hit
TTTTTTGGGGTTTTAAGGCTGTCTCTTATACACATCTGACGCGTCGTAAGT	71	0.11600548983726554	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	65	0.10620220900594733	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.10293444872884125	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	62	0.10130056859028821	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4525847983791909	0.0
2	0.0	0.0	0.0	1.506437487745899	0.0
3	0.0	0.0	0.0	2.155087902751454	0.0
4	0.0	0.0	0.0	3.0896673420037906	0.0
5	0.0	0.0	0.0	4.94085353898438	0.0
6	0.0	0.0	0.0	6.363963139664074	0.0
7	0.0	0.0	0.0	7.698843212861904	0.0
8	0.0	0.0	0.0	9.517351807071433	0.0
9	0.0	0.0	0.0	10.375138879811777	0.0
10	0.0	0.0	0.0	12.494281419515064	0.0
11	0.0	0.0	0.0	17.632834455264362	0.0
12	0.0	0.0	0.0	22.17992288085746	0.0
13	0.0	0.0	0.0	23.753349454284034	0.0
14	0.0	0.0	0.0	24.28926213972943	0.0
15	0.0	0.0	0.0	25.039213123325272	0.0
16	0.0	0.0	0.0	26.919809162799815	0.0
17	0.0	0.0	0.0	29.251356120515	0.0
18	0.0	0.0	0.0	31.319848375923144	0.0
19	0.0	0.0	0.0	32.62368472648846	0.0
20	0.0	0.0	0.0	33.77720410430691	0.0
21	0.0	0.0	0.0	35.185608783739625	0.0
22	0.0	0.0	0.0	36.25253251421476	0.0
23	0.0	0.0	0.0	37.04169662113588	0.0
24	0.0	0.0	0.0	37.50571858048494	0.0
25	0.0	0.0	0.0	37.77694268348474	0.0
26	0.0	0.0	0.0	37.989347101496634	0.0
27	0.0	0.0	0.0	38.16253839618326	0.0
28	0.0	0.0	0.0	38.324292529900006	0.0
29	0.0	0.0	0.0	38.49421606430952	0.0
30	0.0	0.0	0.0	38.655970198026274	0.0
31	0.0	0.0	0.0	38.79975165021894	0.0
32	0.0	0.0	0.0	38.93536370171884	0.0
33	0.0	0.0	0.0	39.05790471211032	0.0
34	0.0	0.0	0.0	39.19841840402588	0.0
35	0.0	0.0	0.0	39.33239657538723	0.0
36	0.0	0.0	0.0	39.44186654467028	0.0
37	0.0	0.0	0.0	39.58238023658584	0.0
38	0.0	0.0	0.0	39.71635840794719	0.0
39	0.0	0.0	0.0	39.84870269916999	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACTT	20	6.9810037E-4	45.000004	25
GGATGGC	20	6.9810037E-4	45.000004	9
GTCTTGG	20	6.9810037E-4	45.000004	2
GGGACCC	20	6.9810037E-4	45.000004	7
TGGGTTA	20	6.9810037E-4	45.000004	6
TGGGTAC	20	6.9810037E-4	45.000004	6
GTTGGGT	20	6.9810037E-4	45.000004	4
GTGCTTG	20	6.9810037E-4	45.000004	1
CTGGGTT	25	3.8475366E-5	45.0	5
GTTTTTG	55	1.8189894E-12	45.0	1
TTGGGTA	35	1.1869088E-7	45.0	5
CCTCTGG	25	3.8475366E-5	45.0	2
TGCTTTG	25	3.8475366E-5	45.0	1
ACTGGGA	25	3.8475366E-5	45.0	42
TGGGACA	25	3.8475366E-5	45.0	6
GCTTTTG	25	3.8475366E-5	45.0	1
TTGCTGG	45	3.7289283E-10	45.0	2
TTTCTTG	30	2.1318792E-6	44.999996	1
CGTTTTT	1730	0.0	44.219654	1
CTTGGGA	80	0.0	42.187504	4
>>END_MODULE