FastQCFastQC Report
Sat 14 Jan 2017
SRR2933590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224520
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79533.542223409941208No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA5620.2503117762337431No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC5230.2329413860680563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.19463744877961875No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2970.13228220203099947No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC2800.12471049349723855No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.12203812577944058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC253.880749E-545.020
CATAGCC253.880749E-545.036
AATTATC207.0211507E-445.045
CTGTCGG302.1576761E-644.9999962
TACGGGA302.1576761E-644.9999964
TACGAAT302.1576761E-644.99999612
GCTACGA302.1576761E-644.99999610
CCTATGC302.1576761E-644.99999635
AGCTACG302.1576761E-644.9999969
CGTTTTT29400.043.4693871
GCATTAG556.002665E-1140.90909216
CGACCAA2050.040.60975629
CGTGCGG501.0732037E-940.52
ACGTGAG1850.040.13513644
AACACGT1850.040.13513641
GAGACAC2150.039.76744523
GACACGA2100.039.64285725
ATGGTCA2100.039.64285713
ACGACCA2100.039.64285728
AAACACG2050.039.51219640
Produced by FastQC (version 0.11.5)