##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224520
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53114199180474	33.0	31.0	34.0	31.0	34.0
2	32.68462052378407	34.0	31.0	34.0	31.0	34.0
3	32.776581150899695	34.0	31.0	34.0	31.0	34.0
4	36.213148049171565	37.0	35.0	37.0	35.0	37.0
5	36.20100659184037	37.0	35.0	37.0	35.0	37.0
6	36.158849991092104	37.0	36.0	37.0	35.0	37.0
7	36.19912257259932	37.0	37.0	37.0	35.0	37.0
8	36.080616426153576	37.0	37.0	37.0	35.0	37.0
9	37.94699804026367	39.0	38.0	39.0	35.0	39.0
10	37.521013718154286	39.0	37.0	39.0	35.0	39.0
11	37.42386424371993	39.0	37.0	39.0	35.0	39.0
12	37.29824959914484	39.0	37.0	39.0	34.0	39.0
13	37.214577765900586	39.0	37.0	39.0	34.0	39.0
14	38.2366470693034	40.0	38.0	41.0	33.0	41.0
15	38.37686620345626	40.0	38.0	41.0	33.0	41.0
16	38.51643060751826	40.0	38.0	41.0	34.0	41.0
17	38.50154551933013	40.0	38.0	41.0	34.0	41.0
18	38.24865490824871	39.0	38.0	41.0	34.0	41.0
19	37.997376625690364	39.0	37.0	41.0	34.0	41.0
20	37.65896579369321	39.0	35.0	41.0	34.0	41.0
21	37.54738107963656	39.0	35.0	41.0	33.0	41.0
22	37.56648405487262	39.0	35.0	41.0	34.0	41.0
23	37.49310529128808	39.0	35.0	41.0	34.0	41.0
24	37.38809460181721	39.0	35.0	41.0	34.0	41.0
25	37.31117940495279	39.0	35.0	41.0	33.0	41.0
26	37.23354266880456	39.0	35.0	41.0	33.0	41.0
27	37.14185818635311	39.0	35.0	41.0	33.0	41.0
28	36.99408961339747	39.0	35.0	41.0	33.0	41.0
29	36.85487707108498	38.0	35.0	40.0	33.0	41.0
30	36.658502583288794	38.0	35.0	40.0	32.0	41.0
31	36.4014608943524	38.0	35.0	40.0	31.0	41.0
32	36.0748797434527	38.0	35.0	41.0	30.0	41.0
33	35.73799661500089	38.0	35.0	41.0	29.0	41.0
34	35.36520131836807	38.0	35.0	41.0	25.0	41.0
35	35.06465793693212	38.0	35.0	41.0	23.0	41.0
36	34.87862996615001	38.0	35.0	40.0	22.0	41.0
37	34.767374844111885	38.0	34.0	40.0	22.0	41.0
38	34.672790842686624	38.0	34.0	40.0	21.0	41.0
39	34.60578567610903	38.0	34.0	40.0	21.0	41.0
40	34.43983609477998	38.0	34.0	40.0	20.0	41.0
41	34.26625690361661	38.0	34.0	40.0	18.0	41.0
42	34.10612862996615	37.0	34.0	40.0	18.0	41.0
43	33.98378763584536	37.0	34.0	40.0	18.0	41.0
44	33.82519597363264	37.0	33.0	40.0	18.0	41.0
45	33.792156600748264	37.0	33.0	40.0	18.0	41.0
46	33.65383930162124	36.0	33.0	40.0	18.0	41.0
47	33.51530375913059	36.0	33.0	40.0	18.0	41.0
48	33.42630946018172	36.0	33.0	40.0	18.0	41.0
49	33.379894886869764	35.0	33.0	40.0	18.0	41.0
50	33.27921343310173	35.0	33.0	40.0	18.0	41.0
51	32.25544717619811	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	5.0
11	3.0
12	5.0
13	8.0
14	9.0
15	10.0
16	24.0
17	40.0
18	69.0
19	125.0
20	193.0
21	351.0
22	569.0
23	940.0
24	1709.0
25	2974.0
26	4747.0
27	5230.0
28	5095.0
29	4679.0
30	4646.0
31	5006.0
32	6030.0
33	8778.0
34	15400.0
35	16674.0
36	21279.0
37	27541.0
38	45366.0
39	47000.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.555763406378052	5.375022269730981	37.560128273650456	30.509086050240512
2	34.22724033493675	5.512649207197577	44.18359166221272	16.07651879565295
3	20.54159985747372	5.494833422412257	56.98111526812756	16.98245145198646
4	16.652414038838412	5.576340637805095	57.019419205416	20.751826117940496
5	15.658293247817568	6.488954213433101	56.35711740602174	21.495635132727596
6	19.517192232317836	7.426064493140923	58.25271690717976	14.804026367361484
7	71.06850169249955	2.939159094958133	21.47247461250668	4.519864600035631
8	71.86264029930518	3.4148405487261715	20.646712987707108	4.075806164261535
9	66.96240869410298	5.042312488865135	22.680830215570996	5.314448601460894
10	36.18964902903973	20.0445394619633	28.619722073757348	15.146089435239624
11	27.080438268305716	20.35720648494566	34.380901478710136	18.181453768038484
12	22.967664350614644	18.531979333689648	39.617406021735256	18.882950293960448
13	22.631391412791736	18.613486549082488	41.178514163548904	17.576607874576876
14	19.234812043470516	19.302957420274364	43.547122750757175	17.915107785497952
15	17.885711740602172	18.042045252093356	44.177356137537856	19.894886869766612
16	21.116158916800284	18.13958667379298	41.056476037769464	19.687778371637272
17	20.672545875645824	18.76135756280064	39.12791733475859	21.43817922679494
18	21.86798503474078	18.235346517014076	40.02583288793871	19.870835560306432
19	21.079191163370748	20.977641190094424	38.41350436486727	19.52966328166756
20	21.794494922501336	23.574737217174416	36.85907714234812	17.771690717976128
21	20.782558346695172	23.817922679494032	38.00062355246749	17.398895421343312
22	20.49973276322822	20.47300908605024	38.03714591127739	20.990112239444148
23	19.002761446641724	23.0264564404062	37.44343488330661	20.527347229645464
24	18.83529306965972	23.77917334758596	37.38776055585248	19.997773026901836
25	20.95002672367718	23.663816141101016	34.601817210048104	20.784339925173704
26	19.642348120434704	22.642526278282556	35.857830037413144	21.857295563869588
27	19.431231070728668	21.460448957776592	37.742740067699984	21.365579903794764
28	18.180117584179584	23.391680028505256	36.08141813646891	22.346784250846248
29	19.91849278460716	22.738731516123284	35.634241938357384	21.708533760912168
30	21.844379119900232	22.228754676643504	36.58738642437199	19.339479779084268
31	22.067967218955996	24.35328701229289	34.102975236059144	19.475770532691968
32	22.0154106538393	23.374309638339568	33.47006948156066	21.140210226260468
33	22.66924995546054	23.293693212186	31.081863531088544	22.95519330126492
34	21.031533939070016	23.87716016390522	33.81525031177623	21.27605558524853
35	21.04667735613754	25.862729378229112	32.434972385533584	20.655620880099768
36	22.976572243007304	27.307589524318548	30.238286121503656	19.477552111170496
37	21.521022626046676	27.192677712453232	30.231605202209156	21.054694459290932
38	23.191252449670408	25.747372171744164	29.373329770176376	21.688045608409052
39	23.371191876002136	24.673080349189384	28.315517548548012	23.640210226260468
40	25.41199002316052	23.469178692321396	29.68466060929984	21.43417067521824
41	22.36281845715304	25.326028861571352	28.977819347942273	23.333333333333332
42	22.03723499020132	26.391858186353108	30.708622839836096	20.86228398360948
43	21.127293782291108	24.833867806876896	32.664350614644576	21.37448779618742
44	22.5432032781044	23.81925886335293	31.29520755389275	22.34233030464992
45	22.61045786566898	22.994388027792624	30.15766969535008	24.237484411188312
46	22.182879030821308	23.576518795652948	31.19098521289863	23.049616960627116
47	19.92740067699982	26.474256190985212	33.00908605024051	20.589257081774452
48	20.567432745412436	26.441742383752004	31.965526456440408	21.025298414395156
49	22.46570461428826	24.35016924995546	31.731248886513452	21.45287724924283
50	20.75004453946196	24.35774095848922	31.15446285408872	23.737751647960092
51	20.450293960448956	24.221450204881524	29.688223766256904	25.640032068412616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	633.0
1	888.0
2	1143.0
3	6787.5
4	12432.0
5	7671.5
6	2911.0
7	2860.0
8	2809.0
9	2823.5
10	2838.0
11	2781.0
12	2724.0
13	2538.0
14	2352.0
15	2163.0
16	1974.0
17	1907.0
18	1840.0
19	1758.5
20	1677.0
21	1625.0
22	1573.0
23	1572.0
24	1571.0
25	1656.0
26	1854.0
27	1967.0
28	2097.5
29	2228.0
30	2643.0
31	3058.0
32	3225.5
33	3393.0
34	3807.0
35	4221.0
36	4300.0
37	4379.0
38	4891.5
39	5404.0
40	5803.0
41	6202.0
42	7050.0
43	7898.0
44	8656.0
45	9414.0
46	11631.5
47	13849.0
48	15877.5
49	17906.0
50	18518.5
51	19131.0
52	17900.0
53	16669.0
54	15052.0
55	13435.0
56	12497.5
57	11560.0
58	10978.5
59	10397.0
60	9664.5
61	8932.0
62	7909.5
63	6887.0
64	6299.0
65	5711.0
66	4987.0
67	4263.0
68	3572.0
69	2881.0
70	2504.5
71	2128.0
72	1819.5
73	1511.0
74	1413.0
75	1031.5
76	748.0
77	593.5
78	439.0
79	327.5
80	216.0
81	158.0
82	100.0
83	61.0
84	22.0
85	22.0
86	22.0
87	14.5
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	224520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.192700745829455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.08676663372502	31.45111635862786
2	12.416325918500162	11.47087255031928
3	6.377044196464461	8.83718682630634
4	3.961105844567042	7.3189670760256575
5	2.621514936029307	6.054742747036202
6	1.830549093252862	5.0734803879107355
7	1.2623086960589407	4.0816613492136185
8	0.8628438354109936	3.1885669663619005
9	0.61717301513427	2.5657999556848754
>10	1.9004534733727423	12.912496670645101
>50	0.04094513526332139	1.284283903054064
>100	0.019973236713815314	1.5915156126208765
>500	0.0019973236713815316	0.5005201274474351
>1k	0.0	0.0
>5k	9.986618356907658E-4	3.668789468746038
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7953	3.542223409941208	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	562	0.2503117762337431	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	523	0.2329413860680563	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	437	0.19463744877961875	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	297	0.13228220203099947	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	280	0.12471049349723855	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.12203812577944058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0681453768038482	0.0
2	0.0	0.0	0.0	0.3295920185284162	0.0
3	0.0	0.0	0.0	0.5037413148049171	0.0
4	0.0	0.0	0.0	0.7901300552289329	0.0
5	0.0	0.0	0.0	1.5187956529485125	0.0
6	0.0	0.0	0.0	2.4719401389631215	0.0
7	0.0	0.0	0.0	3.3132905754498485	0.0
8	0.0	0.0	0.0	4.559504721182968	0.0
9	0.0	0.0	0.0	5.412435417780153	0.0
10	0.0	0.0	0.0	6.74594690896134	0.0
11	0.0	0.0	0.0	8.95154106538393	0.0
12	0.0	0.0	0.0	10.61731694281133	0.0
13	0.0	0.0	0.0	11.319704257972564	0.0
14	0.0	0.0	0.0	11.67022982362373	0.0
15	0.0	0.0	0.0	12.054159985747372	0.0
16	0.0	0.0	0.0	12.66791377160164	0.0
17	0.0	0.0	0.0	13.43087475503296	0.0
18	0.0	0.0	0.0	14.116337074648138	0.0
19	0.0	0.0	0.0	14.5612862996615	0.0
20	0.0	0.0	0.0	14.971940138963122	0.0
21	0.0	0.0	0.0	15.422679494031712	0.0
22	0.0	0.0	0.0	15.85649385355425	0.0
23	0.0	0.0	0.0	16.161589168002852	0.0
24	0.0	0.0	0.0	16.39363976483164	0.0
25	0.0	0.0	0.0	16.550864065562088	0.0
26	0.0	0.0	0.0	16.717441653304828	0.0
27	0.0	0.0	0.0	16.929449492250132	0.0
28	0.0	0.0	0.0	17.09424550151434	0.0
29	0.0	0.0	0.0	17.315161232852308	0.0
30	0.0	0.0	0.0	17.547211829681096	0.0
31	0.0	0.0	0.0	17.70978086584714	0.0
32	0.0	0.0	0.0	17.939159094958132	0.0
33	0.0	0.0	0.0	18.101728131124176	0.0
34	0.0	0.0	0.0	18.299037947621592	0.0
35	0.0	0.0	0.0	18.527079992873688	0.0
36	0.0	0.0	0.0	18.714145733119544	0.0
37	0.0	0.0	0.0	18.902993051843932	0.0
38	0.0	0.0	0.0	19.17468376982006	0.0
39	0.0	0.0	0.0	19.792000712631392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCC	25	3.880749E-5	45.0	20
CATAGCC	25	3.880749E-5	45.0	36
AATTATC	20	7.0211507E-4	45.0	45
CTGTCGG	30	2.1576761E-6	44.999996	2
TACGGGA	30	2.1576761E-6	44.999996	4
TACGAAT	30	2.1576761E-6	44.999996	12
GCTACGA	30	2.1576761E-6	44.999996	10
CCTATGC	30	2.1576761E-6	44.999996	35
AGCTACG	30	2.1576761E-6	44.999996	9
CGTTTTT	2940	0.0	43.469387	1
GCATTAG	55	6.002665E-11	40.909092	16
CGACCAA	205	0.0	40.609756	29
CGTGCGG	50	1.0732037E-9	40.5	2
ACGTGAG	185	0.0	40.135136	44
AACACGT	185	0.0	40.135136	41
GAGACAC	215	0.0	39.767445	23
GACACGA	210	0.0	39.642857	25
ATGGTCA	210	0.0	39.642857	13
ACGACCA	210	0.0	39.642857	28
AAACACG	205	0.0	39.512196	40
>>END_MODULE