##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933589.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	214486
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5141547700083	33.0	31.0	34.0	31.0	34.0
2	32.69051126880076	34.0	31.0	34.0	31.0	34.0
3	32.77233012877297	34.0	31.0	34.0	31.0	34.0
4	36.17194129220555	37.0	35.0	37.0	35.0	37.0
5	36.20212974273379	37.0	35.0	37.0	35.0	37.0
6	36.17526551849538	37.0	37.0	37.0	35.0	37.0
7	36.15706386430816	37.0	37.0	37.0	35.0	37.0
8	36.08380500358998	37.0	37.0	37.0	35.0	37.0
9	37.96920078699775	39.0	38.0	39.0	35.0	39.0
10	37.574722825732216	39.0	37.0	39.0	35.0	39.0
11	37.491579870014824	39.0	37.0	39.0	35.0	39.0
12	37.33457195341421	39.0	37.0	39.0	35.0	39.0
13	37.27148158854191	39.0	37.0	39.0	34.0	39.0
14	38.31839840362541	40.0	38.0	41.0	34.0	41.0
15	38.45052823960538	40.0	38.0	41.0	34.0	41.0
16	38.639025390934606	40.0	38.0	41.0	34.0	41.0
17	38.643911490726666	40.0	38.0	41.0	35.0	41.0
18	38.44167451488675	39.0	38.0	41.0	35.0	41.0
19	38.220182203034234	40.0	37.0	41.0	35.0	41.0
20	37.84201299851738	39.0	35.0	41.0	34.0	41.0
21	37.78422367893476	39.0	35.0	41.0	34.0	41.0
22	37.795170780377276	39.0	35.0	41.0	34.0	41.0
23	37.660905606892754	39.0	35.0	41.0	34.0	41.0
24	37.56564064787445	39.0	35.0	41.0	34.0	41.0
25	37.48927668938765	39.0	35.0	41.0	34.0	41.0
26	37.42696959242095	39.0	35.0	41.0	34.0	41.0
27	37.3407634997156	39.0	35.0	41.0	34.0	41.0
28	37.239055229711965	39.0	35.0	41.0	33.0	41.0
29	37.06033494027582	39.0	35.0	41.0	33.0	41.0
30	36.81624441688502	39.0	35.0	41.0	33.0	41.0
31	36.54774670607872	39.0	35.0	41.0	32.0	41.0
32	36.28255923463536	39.0	35.0	41.0	31.0	41.0
33	35.89631024868756	39.0	35.0	41.0	29.0	41.0
34	35.56109955894557	39.0	35.0	41.0	26.0	41.0
35	35.30680324123719	39.0	35.0	41.0	23.0	41.0
36	35.11455293119364	38.0	35.0	41.0	23.0	41.0
37	35.019045532109324	38.0	35.0	41.0	23.0	41.0
38	34.908408940443664	38.0	35.0	41.0	22.0	41.0
39	34.8698563076378	38.0	35.0	41.0	22.0	41.0
40	34.724336320319274	38.0	35.0	41.0	21.0	41.0
41	34.55314099754762	38.0	35.0	41.0	20.0	41.0
42	34.38265434573818	38.0	34.0	40.0	20.0	41.0
43	34.29310071519819	37.0	34.0	40.0	20.0	41.0
44	34.14818682804472	37.0	34.0	40.0	18.0	41.0
45	34.034650280204765	37.0	34.0	40.0	20.0	41.0
46	33.89885586938075	37.0	34.0	40.0	19.0	41.0
47	33.840474436559965	36.0	34.0	40.0	20.0	41.0
48	33.722438760571784	36.0	34.0	40.0	18.0	41.0
49	33.69277248864728	36.0	34.0	40.0	18.0	41.0
50	33.52594574937292	35.0	33.0	40.0	18.0	41.0
51	32.662998983616646	35.0	32.0	39.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	6.0
11	6.0
12	10.0
13	16.0
14	11.0
15	19.0
16	16.0
17	43.0
18	55.0
19	104.0
20	184.0
21	313.0
22	474.0
23	838.0
24	1534.0
25	2822.0
26	4191.0
27	4927.0
28	4787.0
29	4182.0
30	4053.0
31	4447.0
32	5396.0
33	7819.0
34	14160.0
35	15247.0
36	19334.0
37	25088.0
38	43669.0
39	50711.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.699178501160915	5.500592113238159	37.504079520341655	30.29614986525927
2	34.360750818235225	5.4884701099372455	44.0760702330222	16.07470883880533
3	20.675475322398665	5.547215202857063	56.80184254450174	16.975466930242533
4	16.906930988502747	5.622744608039685	56.59530225749	20.87502214596757
5	15.529684921160355	6.585977639566219	56.38829573958207	21.496041699691354
6	19.600813106682953	7.392557089973238	58.090970972464405	14.915658830879405
7	71.28017679475583	2.9377208768870693	21.275980716690135	4.506121611666962
8	72.12825079492367	3.430526934158873	20.499706274535402	3.941515996382048
9	67.2318939231465	5.0935725408651376	22.45507865315219	5.2194548828361755
10	36.116110142386916	20.19246011394683	28.626110795110172	15.065318948556083
11	27.00409350726854	20.699253098104307	34.126702908348335	18.169950486278825
12	23.00616357244762	18.82080881735871	39.44126889400707	18.731758716186604
13	22.984716951222925	18.7872401928331	40.97050623350708	17.257536622436895
14	19.49171507697472	19.423179135234932	43.16878490903836	17.916320878751993
15	18.011898212470744	18.336861147114497	44.02338614175284	19.627854498661918
16	21.34032058036422	18.190464645711142	40.955586844829035	19.513627929095605
17	20.707645254235707	18.875824063109015	39.10465018695859	21.311880495696688
18	22.049457773467733	18.32473914381358	39.676715496582524	19.94908758613616
19	21.227958934382663	20.806019973331594	38.411364844325504	19.55465624796024
20	22.16088695765691	23.665880290555094	36.523595945656126	17.64963680613187
21	21.190194231791352	23.50642932405845	37.99548688492489	17.307889559225313
22	20.818608207528698	20.42277817666421	37.83277230215492	20.925841313652175
23	19.49824230952137	22.60287384724411	37.59732569957946	20.301558143655065
24	19.080499426535997	23.805749559411804	37.25417975998433	19.859571254067866
25	21.24241209216452	23.564241955185885	34.5444457913337	20.64890016131589
26	19.77052115289576	22.67001109629533	35.589269229693315	21.9701985211156
27	19.470734686646214	21.602342344022453	37.75164812621803	21.175274843113304
28	18.247344815046205	23.46726592877857	35.92402301315703	22.361366243018193
29	19.873091950057347	22.954411942970637	35.57201868653432	21.6004774204377
30	22.105871711906605	22.221963205057673	36.41916022490979	19.253004858125937
31	22.17067780647688	24.349374784368212	34.10851990339696	19.37142750575795
32	21.91611573715767	23.401993603312103	33.457195341420885	21.22469531810934
33	22.95347948117826	23.204777934224143	30.917169418983057	22.92457316561454
34	21.095083128968792	23.939091595721866	33.73180533927622	21.23401993603312
35	21.12492190632489	25.834320188730263	32.38020197122423	20.660555933720616
36	22.884477308542284	27.466594556288054	30.149287132959728	19.499641002209934
37	21.617727963596693	26.956071724961067	30.299413481532593	21.126786829909644
38	23.13064722173009	25.838982497692157	29.446210941506674	21.584159339071082
39	23.578228882071556	24.707440112641386	28.054045485486235	23.660285519800826
40	25.31587143216807	23.709239763900676	29.46579263914661	21.509096164784648
41	22.310547075333588	25.513553332152217	28.748263289911698	23.4276363026025
42	22.23222028477383	26.552781999757556	30.562367706983206	20.652630008485403
43	21.060582042650804	25.0594444392641	32.5303283197971	21.349645198288002
44	22.546926139701426	24.17080835112781	31.138629094672847	22.143636414497916
45	22.650895629551577	23.25466464011637	29.91057691411094	24.183862816221104
46	22.126385871338922	23.7083073021083	31.03465960482269	23.13064722173009
47	19.836259709258414	26.5509170761728	33.08794047163918	20.52488274292961
48	20.54306574788098	26.82086476506625	31.948938392249378	20.68713109480339
49	22.469531810934047	24.66687802467294	31.484106188748918	21.3794839756441
50	20.80788489691635	24.652424866891078	31.025801217795102	23.51388901839747
51	20.75986311460888	24.323732085077815	29.568829667204387	25.34757513310892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	566.0
1	898.0
2	1230.0
3	6472.5
4	11715.0
5	7294.5
6	2874.0
7	2744.0
8	2614.0
9	2620.0
10	2626.0
11	2585.5
12	2545.0
13	2442.0
14	2339.0
15	2168.0
16	1997.0
17	1918.0
18	1839.0
19	1714.0
20	1589.0
21	1529.0
22	1469.0
23	1492.0
24	1515.0
25	1592.5
26	1847.5
27	2025.0
28	2082.0
29	2139.0
30	2560.0
31	2981.0
32	3152.5
33	3324.0
34	3676.5
35	4029.0
36	4104.0
37	4179.0
38	4668.0
39	5157.0
40	5597.0
41	6037.0
42	6796.5
43	7556.0
44	8203.0
45	8850.0
46	10879.5
47	12909.0
48	14754.0
49	16599.0
50	17260.0
51	17921.0
52	16793.0
53	15665.0
54	14232.5
55	12800.0
56	12008.0
57	11216.0
58	10522.0
59	9828.0
60	9312.5
61	8797.0
62	7690.5
63	6584.0
64	6158.5
65	5733.0
66	4970.0
67	4207.0
68	3525.5
69	2844.0
70	2509.0
71	2174.0
72	1813.0
73	1452.0
74	1388.5
75	1059.5
76	794.0
77	614.0
78	434.0
79	317.5
80	201.0
81	145.0
82	89.0
83	59.5
84	30.0
85	19.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	3.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	214486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.84449648010982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73197947912513	33.36291446740092
2	11.779837056833069	11.27200745203834
3	6.126628933266346	8.79376429297802
4	3.8563360269169524	7.380178218637952
5	2.4699032496259954	5.908563866647137
6	1.6852503925935118	4.837797388592638
7	1.1604837580666307	3.886593275463047
8	0.820634937754451	3.141029231267837
9	0.5427585519196805	2.337120866418359
>10	1.7672138485889797	12.364215854253379
>50	0.03698354773630191	1.1812335870659272
>100	0.020990662228711893	1.988967809152709
>500	0.0	0.0
>1k	0.0	0.0
>5k	9.995553442243758E-4	3.545613690083718
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7414	3.4566358643454587	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	486	0.22658821554786793	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	463	0.21586490493552024	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.1706405080051845	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	328	0.1529237339500014	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.13427449810244024	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	268	0.12494988017865968	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	232	0.10816556791585465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07040086532454333	0.0
2	0.0	0.0	0.0	0.31284093134283825	0.0
3	0.0	0.0	0.0	0.47928536128232146	0.0
4	0.0	0.0	0.0	0.7347798923939092	0.0
5	0.0	0.0	0.0	1.4327275439888851	0.0
6	0.0	0.0	0.0	2.3236947866061186	0.0
7	0.0	0.0	0.0	3.087380994563748	0.0
8	0.0	0.0	0.0	4.207733838105984	0.0
9	0.0	0.0	0.0	4.974683662336936	0.0
10	0.0	0.0	0.0	6.166836063892282	0.0
11	0.0	0.0	0.0	8.283524332590472	0.0
12	0.0	0.0	0.0	9.83980306406945	0.0
13	0.0	0.0	0.0	10.479471853640797	0.0
14	0.0	0.0	0.0	10.81888794606641	0.0
15	0.0	0.0	0.0	11.150844344152999	0.0
16	0.0	0.0	0.0	11.740626427832119	0.0
17	0.0	0.0	0.0	12.448831159143253	0.0
18	0.0	0.0	0.0	13.121136111447834	0.0
19	0.0	0.0	0.0	13.536547839952258	0.0
20	4.662308961890287E-4	0.0	0.0	13.983197038501347	0.0
21	4.662308961890287E-4	0.0	0.0	14.440103316766596	0.0
22	4.662308961890287E-4	0.0	0.0	14.861576046921478	0.0
23	4.662308961890287E-4	0.0	0.0	15.16695728392529	0.0
24	4.662308961890287E-4	0.0	0.0	15.3790923416913	0.0
25	4.662308961890287E-4	0.0	0.0	15.526421304887032	0.0
26	4.662308961890287E-4	0.0	0.0	15.684473578695114	0.0
27	4.662308961890287E-4	0.0	0.0	15.884020402264017	0.0
28	4.662308961890287E-4	0.0	0.0	16.05139729399588	0.0
29	4.662308961890287E-4	0.0	0.0	16.255606426526672	0.0
30	4.662308961890287E-4	0.0	0.0	16.4584168663689	0.0
31	4.662308961890287E-4	0.0	0.0	16.62625998899695	0.0
32	4.662308961890287E-4	0.0	0.0	16.841192432140094	0.0
33	4.662308961890287E-4	0.0	0.0	16.99598108967485	0.0
34	4.662308961890287E-4	0.0	0.0	17.195527913243755	0.0
35	4.662308961890287E-4	0.0	0.0	17.455218522421045	0.0
36	4.662308961890287E-4	0.0	0.0	17.627723954010985	0.0
37	4.662308961890287E-4	0.0	0.0	17.799296923808548	0.0
38	4.662308961890287E-4	0.0	0.0	18.044534375203977	0.0
39	4.662308961890287E-4	0.0	0.0	18.67487854685154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	20	7.020444E-4	45.0	22
ATCTACC	35	1.2057535E-7	45.0	15
AACCCGA	20	7.020444E-4	45.0	24
CGCATGG	25	3.880164E-5	45.0	2
CCGTGGC	25	3.880164E-5	45.0	26
CAACCCG	20	7.020444E-4	45.0	23
GGATCGT	20	7.020444E-4	45.0	8
ATCAACC	25	3.880164E-5	45.0	10
GCATTCC	20	7.020444E-4	45.0	12
TACGAAT	70	0.0	45.0	12
CGAATAT	70	0.0	45.0	14
GCTACGA	70	0.0	45.0	10
CCAGTAG	55	1.8189894E-12	45.0	26
AAGCTAC	70	0.0	45.0	8
CGTTTTT	2980	0.0	43.489933	1
TGGGATC	150	0.0	42.000004	6
CCTATGC	75	0.0	42.000004	35
AGCTACG	75	0.0	42.000004	9
ATACTAT	65	0.0	41.538464	45
TAGCATA	60	3.6379788E-12	41.249996	30
>>END_MODULE