FastQCFastQC Report
Sat 14 Jan 2017
SRR2933588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87122
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43715.01710245402998No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC23532.7008103578889373No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG21912.5148642134018964No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC14811.6999150616377035No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC5690.6531071371180643No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT5240.6014554303161085No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA5170.5934207203691375No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC4730.5429168292738918No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC4600.5279952250866601No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT4580.5256995936732398No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC4260.48896949105851567No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT3320.3810748146277634Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC3130.3592663162002709No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT2750.3156493193452859No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC2360.27088450678359083No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT1880.21578935286150455No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATG1530.17561580312664998No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG1350.15495512040586765No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT1150.13199880627166502No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT1000.11478157067101306No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC980.11248593925759282No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.10904249213746242No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG900.10330341360391176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACTG600.045.0000043
GGGGCGA302.1423948E-645.0000046
AATGACT600.045.0000042
ACTAATC206.997397E-445.010
ATGGGAA206.997397E-445.05
GCCTCGA206.997397E-445.014
TCGATCA206.997397E-445.017
CTGGGTA206.997397E-445.05
GCGACCT406.690243E-945.011
CAACCAA206.997397E-445.038
CCTCGAT206.997397E-445.015
ATGAATG850.045.021
GGATTAA206.997397E-445.08
TTGGGTC406.690243E-945.05
ATCAGAA206.997397E-445.020
TGGGGCG351.1947304E-745.05
GGCCTCG206.997397E-445.013
GGCCTAT206.997397E-445.08
TGCTTTG406.690243E-945.01
CTAATCC206.997397E-445.011
Produced by FastQC (version 0.11.5)