##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87122
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80363168889603	33.0	31.0	34.0	30.0	34.0
2	31.906590757787928	33.0	31.0	34.0	30.0	34.0
3	32.02638828309727	33.0	31.0	34.0	30.0	34.0
4	35.810541539450426	37.0	35.0	37.0	35.0	37.0
5	35.80659305341934	37.0	35.0	37.0	35.0	37.0
6	35.563336470696264	37.0	35.0	37.0	33.0	37.0
7	35.79125823557769	37.0	35.0	37.0	35.0	37.0
8	35.32302977433943	37.0	35.0	37.0	32.0	37.0
9	37.28651775670898	39.0	37.0	39.0	34.0	39.0
10	36.97162599573013	39.0	37.0	39.0	33.0	39.0
11	36.99361814467069	39.0	37.0	39.0	33.0	39.0
12	37.02985468653153	39.0	37.0	39.0	33.0	39.0
13	36.86949335414706	39.0	37.0	39.0	33.0	39.0
14	37.89375817818691	40.0	37.0	41.0	33.0	41.0
15	37.92414085994353	40.0	37.0	41.0	33.0	41.0
16	38.05164022864489	40.0	37.0	41.0	33.0	41.0
17	37.94954202153302	40.0	37.0	41.0	33.0	41.0
18	37.70295677326048	39.0	37.0	40.0	33.0	41.0
19	37.38931613140194	38.0	37.0	40.0	33.0	41.0
20	36.75296710360185	38.0	35.0	40.0	32.0	41.0
21	36.72131034641078	38.0	35.0	40.0	32.0	41.0
22	36.81568375381649	38.0	35.0	40.0	32.0	41.0
23	36.61442574780193	38.0	35.0	40.0	32.0	41.0
24	36.59926310231629	38.0	35.0	40.0	32.0	41.0
25	36.4761024769863	38.0	35.0	40.0	32.0	41.0
26	36.43010950161842	38.0	35.0	40.0	32.0	41.0
27	36.32104405316682	38.0	35.0	40.0	31.0	41.0
28	36.249879479350795	38.0	35.0	40.0	31.0	41.0
29	36.149468561327794	38.0	35.0	40.0	31.0	41.0
30	36.074229241752946	38.0	35.0	40.0	31.0	41.0
31	35.80398751176511	38.0	35.0	40.0	30.0	41.0
32	35.19609283533436	38.0	35.0	40.0	26.0	41.0
33	34.829870755951426	38.0	35.0	40.0	23.0	41.0
34	34.40081724478318	38.0	34.0	40.0	20.0	41.0
35	34.10000918252565	38.0	34.0	40.0	18.0	41.0
36	33.853435412410185	38.0	33.0	40.0	16.0	41.0
37	33.76567342347513	38.0	34.0	40.0	15.0	41.0
38	33.882739147402496	38.0	33.0	40.0	16.0	41.0
39	33.78866417208053	38.0	33.0	40.0	15.0	41.0
40	33.7140102385161	38.0	33.0	40.0	15.0	41.0
41	33.412593833934025	38.0	33.0	40.0	15.0	41.0
42	33.307224352058036	37.0	33.0	40.0	13.0	41.0
43	33.10614999655655	37.0	33.0	40.0	12.0	41.0
44	33.036225063703775	37.0	33.0	40.0	11.0	41.0
45	33.174307293221	37.0	33.0	40.0	12.0	41.0
46	32.9058331994215	37.0	32.0	40.0	10.0	41.0
47	32.691926264319	37.0	31.0	40.0	10.0	41.0
48	32.665147723881454	37.0	31.0	40.0	10.0	41.0
49	32.791545189504376	37.0	32.0	40.0	10.0	41.0
50	32.64985881866807	37.0	31.0	40.0	10.0	41.0
51	31.597931636096508	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	9.0
17	22.0
18	34.0
19	79.0
20	137.0
21	233.0
22	451.0
23	680.0
24	996.0
25	1559.0
26	2259.0
27	2455.0
28	2414.0
29	2152.0
30	2327.0
31	2673.0
32	2893.0
33	3743.0
34	5459.0
35	7236.0
36	8895.0
37	10880.0
38	16971.0
39	12561.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.630357429811067	3.9519294782029797	34.502192327999815	29.915520763986137
2	35.032483184499895	17.03702853469847	33.24533412915222	14.68515415164941
3	15.035237942196	17.559284681251576	51.5070820229104	15.89839535364202
4	12.627120589518148	4.4156470237138725	64.42804343334633	18.529188953421638
5	21.595004706044396	5.261587199559239	53.83944353894539	19.30396455545098
6	15.046716099263103	16.808613209063154	57.10957048736255	11.035100204311195
7	54.19756203943895	2.2921879663001308	38.63892013498313	4.871329859277795
8	51.23964096324695	12.180620279607906	29.145336424783636	7.434402332361516
9	47.239503225362135	4.001285553591515	30.691444181722183	18.067767039324167
10	31.754321526135765	15.959229586097656	36.54989554877069	15.73655333899589
11	23.128486490209134	14.504947085695921	41.11475861435688	21.251807809738068
12	17.16673170955671	11.871857854502881	46.79644636257203	24.16496407336838
13	20.3714331626914	14.032047014531345	52.176258579922404	13.420261242854847
14	17.05309795459241	20.221069305112373	44.31372098895801	18.412111751337203
15	10.666651362457245	14.95718647413971	53.527237666720225	20.848924496682812
16	10.437088221115218	16.189940543146392	44.15991368425885	29.213057551479533
17	10.945570579187805	17.932324785932373	47.00305318977985	24.119051445099977
18	11.296802185441106	15.793944124331396	48.60769954776061	24.301554142466884
19	11.951057138265881	19.012419365946602	47.24868575101582	21.7878377447717
20	13.972360597782421	20.30256422028879	49.30786712885379	16.417208053074997
21	12.720093661761666	26.547829480498613	44.187461261219894	16.544615596519822
22	10.308532861963684	18.855168614127315	46.356832946902045	24.479465577006955
23	12.055508367576502	24.198250728862973	41.705883703312594	22.04035720024793
24	13.912674181033493	20.333555244369965	39.948577856339384	25.805192718257157
25	10.63336470696265	28.783774477169942	37.679346204173456	22.903514611693947
26	11.053465255618558	22.3881453593811	43.0878538141916	23.47053557080875
27	16.405729896007898	22.871375771906063	39.884300176763624	20.838594155322422
28	10.152429925851106	21.26213815109846	44.647735359610664	23.937696563439776
29	15.202819035375681	19.06636670416198	41.04244622483414	24.6883680356282
30	18.8873074539152	23.265076559307637	38.570051192580515	19.277564794196643
31	18.20320929271596	21.89114115837561	33.64018273226051	26.26546681664792
32	19.461215307270265	25.15667684396593	33.754964302931526	21.62714354583228
33	18.748421753403274	16.72826610959344	33.3107596244347	31.212552512568582
34	19.022749707306993	20.13039186428227	35.45717499598264	25.38968343242809
35	21.80161153325222	20.682491219209844	32.39939395330686	25.11650329423108
36	20.464406234934916	24.25564151419848	32.58878354491403	22.69116870595257
37	22.468492458850804	18.141227244553615	38.6136681894355	20.776612107160076
38	17.865751474943185	17.22756594201235	33.32568122862193	31.581001354422533
39	17.91166410321159	16.980785565069674	42.160418723169805	22.947131608548933
40	21.060122586717476	16.405729896007898	36.88046647230321	25.65368104497142
41	20.878767705057278	19.090470834002893	38.115516172723304	21.91524528821652
42	24.287780353986363	16.643327747296894	39.789031473106675	19.279860425610064
43	19.6242051376231	16.281765799683203	40.69695369711439	23.397075365579305
44	19.455476228736714	15.5678244301095	38.980969215582746	25.995730125571036
45	20.539014255871077	13.35139230045224	35.84743233626409	30.262161107412595
46	25.91653084180804	17.220679047772087	37.7298500952687	19.132940015151167
47	15.875439039507816	17.708500723123898	46.71954271022244	19.69651752714584
48	18.525745506301508	18.90452468951585	40.346869906567804	22.22285989761484
49	20.659534905075642	12.518078097380684	45.48449300980234	21.337893987741328
50	21.50203163380088	11.782328229379491	39.472234338054676	27.243405798764954
51	19.015862813066732	12.468722021992148	35.21613369757352	33.2992814673676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	334.0
2	489.0
3	3709.5
4	6930.0
5	4208.5
6	1487.0
7	1380.5
8	1274.0
9	1234.0
10	1194.0
11	1158.0
12	1122.0
13	1043.0
14	964.0
15	869.5
16	775.0
17	717.5
18	660.0
19	593.5
20	527.0
21	528.0
22	529.0
23	452.5
24	376.0
25	371.5
26	365.0
27	363.0
28	397.5
29	432.0
30	453.5
31	475.0
32	530.0
33	585.0
34	665.5
35	746.0
36	890.5
37	1035.0
38	1120.0
39	1205.0
40	1492.5
41	1780.0
42	2412.5
43	3045.0
44	3703.5
45	4362.0
46	9717.0
47	15072.0
48	13339.0
49	11606.0
50	10909.0
51	10212.0
52	8247.0
53	6282.0
54	4936.0
55	3590.0
56	2922.5
57	2255.0
58	2014.5
59	1774.0
60	1610.0
61	1446.0
62	1295.0
63	1144.0
64	1019.5
65	895.0
66	755.5
67	616.0
68	542.0
69	468.0
70	399.0
71	330.0
72	276.5
73	223.0
74	180.5
75	108.0
76	78.0
77	61.5
78	45.0
79	35.0
80	25.0
81	19.0
82	13.0
83	8.5
84	4.0
85	3.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	87122.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.87527834530887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68927191421005	41.607171552535526
2	11.296834975904138	11.94646587543904
3	4.749706942213346	7.534262298845297
4	2.114357660747623	4.471889993342669
5	0.9942256762037077	2.628497968366199
6	0.5882863717275213	1.8663483391106725
7	0.3364737550471063	1.2453800417804917
8	0.1932010593496288	0.817244783177613
9	0.16932227673338254	0.8057666261105116
>10	0.7814874310771501	7.417185096760864
>50	0.04558676681283376	1.7882968710543834
>100	0.026049581035905007	4.090815178714906
>500	0.006512395258976252	1.8479832878033102
>1k	0.008683193678635003	11.932692086958518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4371	5.01710245402998	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	2353	2.7008103578889373	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	2191	2.5148642134018964	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1481	1.6999150616377035	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	569	0.6531071371180643	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	524	0.6014554303161085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	517	0.5934207203691375	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	473	0.5429168292738918	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	460	0.5279952250866601	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	458	0.5256995936732398	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	426	0.48896949105851567	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	332	0.3810748146277634	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	313	0.3592663162002709	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	275	0.3156493193452859	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	236	0.27088450678359083	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT	188	0.21578935286150455	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATG	153	0.17561580312664998	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	135	0.15495512040586765	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	115	0.13199880627166502	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	100	0.11478157067101306	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	98	0.11248593925759282	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95	0.10904249213746242	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	90	0.10330341360391176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41206583870893687	0.0
2	0.0	0.0	0.0	1.9799820940749753	0.0
3	0.0	0.0	0.0	2.7191754091962994	0.0
4	0.0	0.0	0.0	4.009320263538486	0.0
5	0.0	0.0	0.0	7.841876908243613	0.0
6	0.0	0.0	0.0	9.424714767796882	0.0
7	0.0	0.0	0.0	11.299097816854525	0.0
8	0.0	0.0	0.0	13.815109845963132	0.0
9	0.0	0.0	0.0	14.797640090907004	0.0
10	0.0	0.0	0.0	19.65519616170428	0.0
11	0.0	0.0	0.0	24.953513463878238	0.0
12	0.0	0.0	0.0	32.297238355409654	0.0
13	0.0	0.0	0.0	33.971901471499734	0.0
14	0.0	0.0	0.0	34.75241615206262	0.0
15	0.0	0.0	0.0	36.29163701476091	0.0
16	0.0	0.0	0.0	38.40361791510755	0.0
17	0.0	0.0	0.0	40.9919423337389	0.0
18	0.0	0.0	0.0	43.201487569155894	0.0
19	0.0	0.0	0.0	45.287068708248206	0.0
20	0.0	0.0	0.0	46.55425724845619	0.0
21	0.0	0.0	0.0	47.867358416932575	0.0
22	0.0	0.0	0.0	48.927940129932736	0.0
23	0.0	0.0	0.0	49.71189825761576	0.0
24	0.0	0.0	0.0	50.18479832878033	0.0
25	0.0	0.0	0.0	50.44305686279011	0.0
26	0.0	0.0	0.0	50.69442850255963	0.0
27	0.0	0.0	0.0	50.86200959573931	0.0
28	0.0	0.0	0.0	51.00663437478478	0.0
29	0.0	0.0	0.0	51.204058676338924	0.0
30	0.0	0.0	0.0	51.388857005119256	0.0
31	0.0	0.0	0.0	51.562177176832485	0.0
32	0.0	0.0	0.0	51.698767245930995	0.0
33	0.0	0.0	0.0	51.83306168361608	0.0
34	0.0	0.0	0.0	51.97079956842129	0.0
35	0.0	0.0	0.0	52.10279837469296	0.0
36	0.0	0.0	0.0	52.229058102431075	0.0
37	0.0	0.0	0.0	52.34383967310209	0.0
38	0.0	0.0	0.0	52.48042974220059	0.0
39	0.0	0.0	0.0	52.64456738826014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGACTG	60	0.0	45.000004	3
GGGGCGA	30	2.1423948E-6	45.000004	6
AATGACT	60	0.0	45.000004	2
ACTAATC	20	6.997397E-4	45.0	10
ATGGGAA	20	6.997397E-4	45.0	5
GCCTCGA	20	6.997397E-4	45.0	14
TCGATCA	20	6.997397E-4	45.0	17
CTGGGTA	20	6.997397E-4	45.0	5
GCGACCT	40	6.690243E-9	45.0	11
CAACCAA	20	6.997397E-4	45.0	38
CCTCGAT	20	6.997397E-4	45.0	15
ATGAATG	85	0.0	45.0	21
GGATTAA	20	6.997397E-4	45.0	8
TTGGGTC	40	6.690243E-9	45.0	5
ATCAGAA	20	6.997397E-4	45.0	20
TGGGGCG	35	1.1947304E-7	45.0	5
GGCCTCG	20	6.997397E-4	45.0	13
GGCCTAT	20	6.997397E-4	45.0	8
TGCTTTG	40	6.690243E-9	45.0	1
CTAATCC	20	6.997397E-4	45.0	11
>>END_MODULE