##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933587.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87936
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704489628820962	33.0	31.0	34.0	30.0	34.0
2	31.842772016011644	33.0	31.0	34.0	30.0	34.0
3	31.97697188864629	33.0	31.0	34.0	30.0	34.0
4	35.71646424672489	37.0	35.0	37.0	35.0	37.0
5	35.77247088791849	37.0	35.0	37.0	35.0	37.0
6	35.58235534934498	37.0	35.0	37.0	33.0	37.0
7	35.70528566229986	37.0	35.0	37.0	35.0	37.0
8	35.285014101164485	37.0	35.0	37.0	32.0	37.0
9	37.26545442139738	39.0	37.0	39.0	34.0	39.0
10	36.987320323871906	39.0	37.0	39.0	33.0	39.0
11	37.00071643013101	39.0	37.0	39.0	33.0	39.0
12	37.000659570596795	39.0	37.0	39.0	33.0	39.0
13	36.8856441048035	39.0	37.0	39.0	33.0	39.0
14	37.93586244541485	40.0	37.0	41.0	33.0	41.0
15	37.91300491266376	40.0	37.0	41.0	33.0	41.0
16	38.13158433406114	40.0	37.0	41.0	33.0	41.0
17	38.06215884279476	40.0	37.0	41.0	33.0	41.0
18	37.877513191411936	39.0	37.0	40.0	33.0	41.0
19	37.60120997088792	39.0	37.0	40.0	34.0	41.0
20	36.90212199781659	38.0	35.0	40.0	32.0	41.0
21	36.915131459243085	38.0	35.0	40.0	33.0	41.0
22	36.9735375727802	38.0	35.0	40.0	33.0	41.0
23	36.731213609898106	38.0	35.0	40.0	32.0	41.0
24	36.71372361717613	38.0	35.0	40.0	32.0	41.0
25	36.622566411935956	38.0	35.0	40.0	32.0	41.0
26	36.619507368995635	38.0	35.0	40.0	32.0	41.0
27	36.50644787117904	38.0	35.0	40.0	32.0	41.0
28	36.52709925400291	38.0	35.0	40.0	32.0	41.0
29	36.31809497816594	38.0	35.0	40.0	31.0	41.0
30	36.17139737991266	38.0	35.0	40.0	31.0	41.0
31	35.91566593886463	38.0	35.0	40.0	30.0	41.0
32	35.42032842066958	38.0	35.0	40.0	27.0	41.0
33	34.94516466521106	38.0	35.0	40.0	23.0	41.0
34	34.54821688500728	38.0	35.0	40.0	21.0	41.0
35	34.28942640101892	38.0	34.0	40.0	18.0	41.0
36	34.0064933588064	38.0	34.0	40.0	17.0	41.0
37	33.92043076783115	38.0	34.0	40.0	15.0	41.0
38	33.941741721251816	38.0	34.0	40.0	15.0	41.0
39	33.8841316411936	38.0	34.0	40.0	15.0	41.0
40	33.845398926491995	38.0	33.0	40.0	15.0	41.0
41	33.55302720160117	38.0	33.0	40.0	15.0	41.0
42	33.377774745269285	38.0	33.0	40.0	13.0	41.0
43	33.19999772561863	38.0	33.0	40.0	12.0	41.0
44	33.16682587336245	37.0	33.0	40.0	12.0	41.0
45	33.246429221251816	37.0	33.0	40.0	13.0	41.0
46	32.97769969068413	37.0	32.0	40.0	12.0	41.0
47	32.82610080058224	37.0	32.0	40.0	10.0	41.0
48	32.80788300582242	37.0	32.0	40.0	11.0	41.0
49	32.88350618631732	37.0	32.0	40.0	10.0	41.0
50	32.63449554221252	37.0	31.0	40.0	10.0	41.0
51	31.799547398107716	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	3.0
14	1.0
15	6.0
16	12.0
17	15.0
18	37.0
19	77.0
20	141.0
21	253.0
22	485.0
23	707.0
24	1028.0
25	1557.0
26	2205.0
27	2387.0
28	2386.0
29	2305.0
30	2381.0
31	2505.0
32	2928.0
33	3618.0
34	5183.0
35	7033.0
36	8622.0
37	10019.0
38	16929.0
39	15108.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.687818413391554	4.259916302765648	34.33974708879185	29.712518195050947
2	34.62290756914119	17.26937772925764	33.7245269286754	14.383187772925766
3	15.174672489082969	17.636690320232898	51.3680403930131	15.820596797671033
4	12.944641557496361	4.502137918486172	64.20123726346434	18.35198326055313
5	22.255958879184863	5.15488537117904	53.063591703056765	19.52556404657933
6	14.949508733624453	16.83383369723435	56.99372270742358	11.222934861717611
7	54.21329148471615	2.253911935953421	38.33924672489083	5.193549854439593
8	51.38509825327511	12.195232896652112	29.05635917030568	7.363309679767103
9	47.36399199417758	3.9801673944687046	30.39596979621543	18.25987081513828
10	31.793577147016013	15.73530749636099	36.35030021834061	16.12081513828239
11	23.20210152838428	14.454830786026202	41.22316229985444	21.11990538573508
12	17.368313318777293	12.001910480349345	46.53384279475983	24.095933406113538
13	20.705967976710333	14.007914847161572	51.82860262008734	13.457514556040756
14	16.8656750363901	20.770787845705968	43.929676128093156	18.43386098981077
15	10.730531295487628	15.076874090247452	53.11476528384279	21.077829330422126
16	10.575873362445416	16.457423580786028	43.74886280931587	29.217840247452692
17	10.970478529839884	18.28830058224163	46.44173034934498	24.299490538573508
18	11.601619359534206	15.900200145560408	48.07359898107715	24.42458151382824
19	11.799490538573508	19.15370269286754	46.761280931586604	22.285525836972344
20	14.332014192139738	20.458060407569143	48.88896470160116	16.32096069868996
21	12.978757278020378	26.368040393013096	44.09911754002911	16.55408478893741
22	10.426901382823871	18.641966885007278	46.23931040756914	24.691821324599708
23	12.348753639010189	24.058406113537117	41.32550946142649	22.2673307860262
24	14.12049672489083	20.166939592430857	40.07232532751092	25.640238355167394
25	10.8738173216885	28.783433406113534	37.427219796215425	22.91552947598253
26	11.036435589519652	22.284388646288207	43.181404657933044	23.497771106259098
27	16.519969068413392	22.797261644832606	39.682268922852984	21.00050036390102
28	10.479212154294032	21.325736899563317	44.295851528384276	23.89919941775837
29	15.302037845705968	19.152565502183407	40.996861353711786	24.548535298398836
30	18.838700873362445	23.73885553129549	38.375636826783115	19.04680676855895
31	18.406568413391557	21.608897379912666	33.38450691411936	26.600027292576417
32	19.675673216885006	24.888555312954878	34.09411390101892	21.34165756914119
33	18.93536208151383	16.76560225618632	33.07973981077147	31.219295851528383
34	18.777292576419214	20.302265283842793	35.53266011644833	25.387782023289667
35	22.510689592430857	20.27497270742358	32.87504548762736	24.339292212518195
36	20.203329694323145	24.176673944687042	32.62258915574964	22.997407205240176
37	22.03420669577875	18.25418486171761	38.642876637554586	21.068731804949053
38	17.95396652110626	17.21934133915575	33.4561499272198	31.370542212518192
39	17.99490538573508	17.209106622998544	41.629139374090244	23.16684861717613
40	21.028930131004365	16.22316229985444	37.001910480349345	25.74599708879185
41	21.19041120815138	19.17417212518195	37.901428311499274	21.733988355167394
42	23.982214337700146	16.938455240174672	39.556040756914115	19.523289665211063
43	19.70524017467249	16.699645196506548	40.24745269286754	23.347661935953422
44	20.27497270742358	15.279294032023289	38.46774927219796	25.97798398835517
45	20.28634461426492	13.664483260553128	35.87836608442503	30.170806040756915
46	25.7107441775837	17.445642285298398	37.613719068413396	19.22989446870451
47	16.274335880640468	18.038118631732168	46.0903384279476	19.597207059679768
48	18.64537845705968	18.580558588064047	40.67617358078602	22.097889374090247
49	20.67753820960699	12.699008369723435	45.036162663755455	21.58729075691412
50	21.229075691411936	11.840429403202329	39.867631004366814	27.062863901018925
51	19.259461426491995	12.481804949053856	35.435998908296945	32.82273471615721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	345.0
2	514.0
3	3697.0
4	6880.0
5	4188.5
6	1497.0
7	1446.5
8	1396.0
9	1289.5
10	1183.0
11	1141.0
12	1099.0
13	1017.0
14	935.0
15	870.5
16	806.0
17	707.0
18	608.0
19	579.0
20	550.0
21	499.5
22	449.0
23	427.5
24	406.0
25	386.5
26	368.5
27	370.0
28	394.0
29	418.0
30	456.0
31	494.0
32	559.5
33	625.0
34	708.0
35	791.0
36	895.0
37	999.0
38	1111.5
39	1224.0
40	1577.5
41	1931.0
42	2502.0
43	3073.0
44	3713.0
45	4353.0
46	9642.5
47	14932.0
48	13298.0
49	11664.0
50	10991.5
51	10319.0
52	8303.5
53	6288.0
54	4947.0
55	3606.0
56	2985.5
57	2365.0
58	2131.5
59	1898.0
60	1719.0
61	1540.0
62	1365.5
63	1191.0
64	1035.5
65	880.0
66	771.5
67	663.0
68	593.5
69	524.0
70	450.0
71	376.0
72	313.5
73	251.0
74	199.5
75	106.0
76	64.0
77	53.0
78	42.0
79	32.5
80	23.0
81	18.0
82	13.0
83	8.0
84	3.0
85	1.5
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	87936.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.28989264919942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96971895606154	42.08287845705968
2	11.237489596892939	11.9768922852984
3	4.57736710697595	7.317822052401747
4	2.1275687672051387	4.535116448326056
5	0.9773585710932332	2.604166666666667
6	0.5591003179616312	1.7876637554585153
7	0.2923539830562728	1.090565866084425
8	0.2262008919997439	0.9643377001455604
9	0.17071765433942937	0.8187772925764192
>10	0.7618275324897036	7.1517922125181945
>50	0.053349266981071675	1.9116175400291122
>100	0.03414353086788587	4.90811499272198
>500	0.004267941358485734	1.2736535662299855
>1k	0.008535882716971468	11.576601164483261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4278	4.8649017467248905	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	2244	2.5518558951965065	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	2108	2.397197962154294	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1550	1.7626455604075693	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	594	0.6754912663755459	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	526	0.5981622998544396	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	479	0.5447143377001455	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	469	0.5333424308588064	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	454	0.5162845705967977	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	425	0.4833060407569141	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	398	0.4526018922852984	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	351	0.39915393013100436	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	284	0.322962154294032	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	245	0.27861171761280934	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	230	0.2615538573508006	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT	190	0.21606622998544397	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	163	0.18536208151382824	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATG	158	0.17967612809315867	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	128	0.1455604075691412	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	120	0.13646288209606985	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115	0.1307769286754003	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	107	0.12167940320232896	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	100	0.11371906841339155	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	99	0.11258187772925765	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	94	0.10689592430858806	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	90	0.1023471615720524	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42530931586608445	0.0
2	0.0	0.0	0.0	1.8547580058224162	0.0
3	0.0	0.0	0.0	2.692867540029112	0.0
4	0.0	0.0	0.0	3.9540120087336246	0.0
5	0.0	0.0	0.0	7.793167758369724	0.0
6	0.0	0.0	0.0	9.450054585152838	0.0
7	0.0	0.0	0.0	11.318458879184861	0.0
8	0.0	0.0	0.0	13.72247998544396	0.0
9	0.0	0.0	0.0	14.679994541484715	0.0
10	0.0	0.0	0.0	19.549445050946144	0.0
11	0.0	0.0	0.0	24.70091885007278	0.0
12	0.0	0.0	0.0	31.707150655021834	0.0
13	0.0	0.0	0.0	33.36176310043668	0.0
14	0.0	0.0	0.0	34.114583333333336	0.0
15	0.0	0.0	0.0	35.54971797671033	0.0
16	0.0	0.0	0.0	37.67512736535662	0.0
17	0.0	0.0	0.0	40.20992540029112	0.0
18	0.0	0.0	0.0	42.311453784570595	0.0
19	0.0	0.0	0.0	44.48348799126637	0.0
20	0.0	0.0	0.0	45.65593158660844	0.0
21	0.0	0.0	0.0	46.96711244541485	0.0
22	0.0	0.0	0.0	48.07814774381368	0.0
23	0.0	0.0	0.0	48.85143740902475	0.0
24	0.0	0.0	0.0	49.313136826783115	0.0
25	0.0	0.0	0.0	49.587199781659386	0.0
26	0.0	0.0	0.0	49.8157751091703	0.0
27	0.0	0.0	0.0	50.01137190684134	0.0
28	0.0	0.0	0.0	50.17512736535662	0.0
29	0.0	0.0	0.0	50.38778202328967	0.0
30	0.0	0.0	0.0	50.58110443959243	0.0
31	0.0011371906841339155	0.0	0.0	50.72552765647744	0.0
32	0.0011371906841339155	0.0	0.0	50.85061863173217	0.0
33	0.0011371906841339155	0.0	0.0	50.9859443231441	0.0
34	0.0011371906841339155	0.0	0.0	51.13036754002911	0.0
35	0.0011371906841339155	0.0	0.0	51.245223799126634	0.0
36	0.0011371906841339155	0.0	0.0	51.37372634643377	0.0
37	0.0011371906841339155	0.0	0.0	51.502228893740906	0.0
38	0.0011371906841339155	0.0	0.0	51.6330058224163	0.0
39	0.0011371906841339155	0.0	0.0	51.79221251819505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTT	20	6.9977494E-4	45.000004	9
CCGCACT	20	6.9977494E-4	45.000004	34
GATGAAT	120	0.0	45.000004	20
GCTACGG	20	6.9977494E-4	45.000004	5
TCTGTTG	20	6.9977494E-4	45.000004	1
CCCGCAC	20	6.9977494E-4	45.000004	33
CGGGATT	20	6.9977494E-4	45.000004	6
GCATGGG	40	6.692062E-9	45.000004	3
TGCTACG	20	6.9977494E-4	45.000004	4
CTACGGC	20	6.9977494E-4	45.000004	6
TGGGTAC	30	2.1426222E-6	45.000004	6
GTTGGGT	20	6.9977494E-4	45.000004	4
GGGAACT	30	2.1426222E-6	45.000004	7
CGGTTTT	20	6.9977494E-4	45.000004	1
CGATGAA	120	0.0	45.000004	19
TAGGGAT	20	6.9977494E-4	45.000004	5
CGCACTT	20	6.9977494E-4	45.000004	35
AATGCTA	20	6.9977494E-4	45.000004	2
TTTATGG	20	6.9977494E-4	45.000004	2
TTCGGGA	20	6.9977494E-4	45.000004	4
>>END_MODULE