##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449942
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30771299411924	33.0	31.0	34.0	30.0	34.0
2	32.502791470900696	34.0	31.0	34.0	31.0	34.0
3	32.56363709100284	34.0	31.0	34.0	31.0	34.0
4	36.09385876401847	37.0	35.0	37.0	35.0	37.0
5	36.09764814131599	37.0	35.0	37.0	35.0	37.0
6	36.07059798818514	37.0	35.0	37.0	35.0	37.0
7	36.129758946708684	37.0	35.0	37.0	35.0	37.0
8	36.00301816678594	37.0	35.0	37.0	35.0	37.0
9	37.8379746722911	39.0	38.0	39.0	35.0	39.0
10	37.28976845904583	39.0	37.0	39.0	34.0	39.0
11	37.25054340337199	39.0	37.0	39.0	34.0	39.0
12	37.263022789603994	39.0	37.0	39.0	34.0	39.0
13	37.25175689311067	39.0	37.0	39.0	34.0	39.0
14	38.5310262211574	40.0	38.0	41.0	34.0	41.0
15	38.57243822537127	40.0	38.0	41.0	34.0	41.0
16	38.59205186446253	40.0	38.0	41.0	34.0	41.0
17	38.53591796275965	40.0	38.0	41.0	34.0	41.0
18	38.09260749163226	39.0	38.0	40.0	34.0	41.0
19	37.63549301910024	38.0	37.0	40.0	34.0	41.0
20	37.10622924732521	38.0	35.0	40.0	34.0	41.0
21	36.997895284281086	38.0	35.0	40.0	33.0	41.0
22	36.97645474305577	38.0	35.0	40.0	33.0	41.0
23	36.895066475234586	38.0	35.0	40.0	33.0	41.0
24	36.784305532713105	38.0	35.0	40.0	33.0	41.0
25	36.69026674549164	38.0	35.0	40.0	33.0	41.0
26	36.60926963919794	38.0	35.0	40.0	33.0	41.0
27	36.52442314787239	38.0	35.0	40.0	33.0	41.0
28	36.35321663681097	38.0	35.0	40.0	32.0	41.0
29	36.20996928493006	38.0	35.0	40.0	31.0	41.0
30	36.01090585008735	38.0	35.0	40.0	31.0	41.0
31	35.62401153926506	38.0	35.0	40.0	30.0	41.0
32	35.00616523907526	37.0	35.0	40.0	24.0	41.0
33	34.21553000164466	37.0	34.0	40.0	18.0	41.0
34	33.52330078098955	38.0	33.0	40.0	15.0	41.0
35	33.01278831493837	37.0	33.0	40.0	10.0	41.0
36	32.737530615057054	37.0	33.0	40.0	10.0	41.0
37	32.52753688253153	37.0	32.0	40.0	10.0	41.0
38	32.38074907432514	37.0	31.0	40.0	10.0	41.0
39	32.303430219895006	37.0	31.0	40.0	9.0	41.0
40	32.13635090745029	37.0	31.0	40.0	8.0	41.0
41	31.96368643069551	36.0	30.0	40.0	8.0	41.0
42	31.798456245471638	36.0	30.0	40.0	8.0	41.0
43	31.640771477212617	36.0	30.0	40.0	8.0	41.0
44	31.489927590667243	36.0	29.0	40.0	8.0	41.0
45	31.450889225722428	35.0	29.0	40.0	8.0	41.0
46	31.26570535757942	35.0	28.0	40.0	7.0	41.0
47	31.157240266523242	35.0	27.0	40.0	7.0	41.0
48	31.058276400069342	35.0	27.0	40.0	7.0	41.0
49	31.014904143200678	35.0	27.0	40.0	7.0	41.0
50	30.909890608122826	35.0	27.0	40.0	7.0	41.0
51	29.989143045103592	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	3.0
12	4.0
13	10.0
14	14.0
15	26.0
16	49.0
17	95.0
18	192.0
19	383.0
20	700.0
21	1080.0
22	1712.0
23	2892.0
24	5498.0
25	10737.0
26	17533.0
27	19467.0
28	17094.0
29	14260.0
30	12401.0
31	12454.0
32	13603.0
33	16567.0
34	24799.0
35	32391.0
36	39901.0
37	49473.0
38	79256.0
39	77315.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.367865191513573	4.21854372341324	37.66463233038925	36.74895875468393
2	42.91153081952785	4.005405141107076	39.49597948179988	13.587084557565197
3	17.463806446164174	4.086971209622574	62.74119775437723	15.708024589836022
4	13.993581394935347	4.2774402034039944	62.945890803703584	18.78308759795707
5	13.054349227233732	5.270901582870681	62.66763271710576	19.007116472789825
6	16.540354090082722	6.012330478150517	64.66033399860427	12.786981433162497
7	62.44538184921612	2.2594023229660714	31.60540691911402	3.6898089087037884
8	63.46729133977268	2.608113934684915	30.141884954060743	3.7827097714816578
9	58.788910570695776	4.123642602824364	32.21126278498117	4.876184041498682
10	28.833938596530217	18.2783558769797	38.25626414071147	14.631441385778613
11	20.51842237443937	19.598303781376266	43.205346466877955	16.67792737730641
12	17.789403967622494	17.31289810686711	47.561463477514884	17.33623444799552
13	17.609158513763994	17.197549906432386	49.60217094647755	15.591120633326073
14	16.34499557720773	18.004987309475442	49.2041196420872	16.445897471229625
15	15.162843210902738	18.44215476661436	49.826199821310304	16.568802201172595
16	15.606900444946238	17.28067173102311	48.78362100004001	18.328806823990647
17	15.620457747887507	18.021433873699277	47.190749029874965	19.167359348538255
18	16.44789772904063	17.63871787919332	47.920620880024536	17.992763511741515
19	17.239777571331416	20.511310346666903	45.95347844833334	16.295433633668342
20	18.829982531081786	20.15059718808202	44.80733072262647	16.212089558209726
21	17.88497184081504	21.435651706219915	44.57796782696437	16.101408626000683
22	16.994857114917032	19.961461699507936	43.96900044894675	19.074680736628277
23	16.145192046974945	20.798902969716096	43.6376244049233	19.41828057838566
24	16.0367336234448	20.522422890061385	44.79710718270355	18.643736303790266
25	16.618586395579875	21.321859261860418	43.34825377493099	18.711300567628715
26	16.23920416409226	21.72635584141956	43.77853145516533	18.255908539322846
27	16.211867307341834	20.9833711900645	44.06190131172462	18.742860190869045
28	15.85270990483218	20.721337416822614	44.200585853287755	19.225366825057453
29	16.804832622871395	20.080588164696785	43.07888572304875	20.03569348938308
30	17.964982153255306	20.159042721061827	42.80929542029862	19.06667970538425
31	17.043974556720645	22.494454840846153	40.40876379622262	20.052806806210576
32	16.871730134106176	22.3968867098426	39.30817749843313	21.423205657618094
33	17.343346475767987	22.689813353721146	38.01378844384387	21.953051726666992
34	16.46301078805713	22.879837845766787	37.1358975156798	23.521253850496286
35	16.68859541896511	23.90085833285179	36.28023167430469	23.13031457387841
36	16.820167932755776	25.57774113107912	34.735365891603806	22.8667250445613
37	16.98530032759778	26.538531632966027	34.964951038133805	21.51121700130239
38	17.18799311911313	26.90969058234172	33.90281414048922	21.99950215805593
39	17.32245489418636	25.71020264834134	33.55165776922359	23.41568468824871
40	18.260353556680638	25.220139484644687	35.34455552048931	21.174951438185367
41	16.610807615203736	26.678994181472277	34.55423143427375	22.155966769050234
42	17.322232643318472	26.83790355201337	33.830138106689304	22.00972569797885
43	17.504033853252196	25.262589400411606	35.033182054575924	22.200194691760274
44	18.249907765889827	25.16857728329429	33.602330967102425	22.979183983713455
45	18.93110667597157	24.384476221379643	33.73212547394998	22.95229162869881
46	18.581728311649055	25.31704086304457	34.370429966529024	21.730800858777354
47	17.338234705806528	26.27294184583791	35.17253334874273	21.216290099612838
48	17.29289552875704	25.318152117384017	35.42100981904334	21.9679425348156
49	18.62484498001965	24.11710842730841	35.134972952069376	22.123073640602566
50	17.695614101373067	23.563481515395317	35.20209271417205	23.538811669059566
51	17.180436589604884	23.69749878873277	33.986380466815724	25.135684154846626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	897.0
2	1446.0
3	17473.5
4	33501.0
5	21960.5
6	10420.0
7	10111.5
8	9803.0
9	9653.0
10	9503.0
11	9179.0
12	8855.0
13	8633.0
14	8411.0
15	8061.0
16	7711.0
17	7208.5
18	6706.0
19	6381.0
20	6056.0
21	5699.0
22	5342.0
23	5334.0
24	5326.0
25	5183.0
26	5179.5
27	5319.0
28	5481.5
29	5644.0
30	5876.0
31	6108.0
32	6772.5
33	7437.0
34	8193.5
35	8950.0
36	9575.0
37	10200.0
38	10839.0
39	11478.0
40	12494.5
41	13511.0
42	15289.5
43	17068.0
44	19644.5
45	22221.0
46	25771.5
47	29322.0
48	33045.5
49	36769.0
50	35541.5
51	34314.0
52	30149.5
53	25985.0
54	22318.0
55	18651.0
56	17044.0
57	15437.0
58	14376.5
59	13316.0
60	12590.0
61	11864.0
62	10874.0
63	9884.0
64	8831.5
65	7779.0
66	6897.5
67	6016.0
68	5281.0
69	4546.0
70	3913.0
71	3280.0
72	2881.5
73	2483.0
74	2095.5
75	1335.0
76	962.0
77	749.5
78	537.0
79	448.0
80	359.0
81	279.0
82	199.0
83	141.0
84	83.0
85	50.0
86	17.0
87	14.0
88	11.0
89	9.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.48625437790052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23069617071187	38.71344130864095
2	8.6238342840219	8.535225141839348
3	3.636609554807282	5.398865565068902
4	2.1864265375217307	4.327922392575704
5	1.5768691795783816	3.901667467064354
6	1.1989208775460305	3.5598062115131155
7	0.8950783750192608	3.1005853308052687
8	0.6807257751321021	2.6949255095824567
9	0.5535874470632038	2.4655472303204022
>10	2.329680841414682	17.653289920376345
>50	0.06152421094460549	2.0607738197353958
>100	0.024699500743836807	2.449905164295776
>500	8.981636634122477E-4	0.3255724841884354
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.4908183170612383E-4	4.8124724539935615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21655	4.8128425441501355	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	928	0.20624880540158508	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	537	0.11934871605673619	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	490	0.10890292526592317	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	484	0.10756942005858533	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1329060189980042	0.0
2	0.0	0.0	0.0	0.4289441750270035	0.0
3	0.0	0.0	0.0	0.7092025194358385	0.0
4	0.0	0.0	0.0	1.0774722075289704	0.0
5	0.0	0.0	0.0	1.9586968987113895	0.0
6	0.0	0.0	0.0	3.2455294237923997	0.0
7	0.0	0.0	0.0	4.345671219846113	0.0
8	0.0	0.0	0.0	5.846753581572736	0.0
9	0.0	0.0	0.0	6.797320543536722	0.0
10	0.0	0.0	0.0	8.235505909650577	0.0
11	0.0	0.0	0.0	10.476461410581809	0.0
12	0.0	0.0	0.0	12.251579092416355	0.0
13	0.0	0.0	0.0	13.040569673424574	0.0
14	0.0	0.0	0.0	13.392615048161764	0.0
15	0.0	0.0	0.0	13.796222624249348	0.0
16	0.0	0.0	0.0	14.520093700965901	0.0
17	0.0	0.0	0.0	15.444212809651022	0.0
18	0.0	0.0	0.0	16.352329855848087	0.0
19	0.0	0.0	0.0	16.859728587240134	0.0
20	0.0	0.0	0.0	17.309119842112985	0.0
21	0.0	0.0	0.0	17.831853883389414	0.0
22	0.0	0.0	0.0	18.305692733730126	0.0
23	0.0	0.0	0.0	18.719079348004854	0.0
24	0.0	0.0	0.0	19.044010116859507	0.0
25	0.0	0.0	0.0	19.302265625347268	0.0
26	0.0	0.0	0.0	19.52851700885892	0.0
27	0.0	0.0	0.0	19.75676865018158	0.0
28	0.0	0.0	0.0	19.979464019806997	0.0
29	2.222508678896391E-4	0.0	0.0	20.200381382489297	0.0
30	2.222508678896391E-4	0.0	0.0	20.449524605393584	0.0
31	2.222508678896391E-4	0.0	0.0	20.67355348022634	0.0
32	2.222508678896391E-4	0.0	0.0	20.918029434904945	0.0
33	2.222508678896391E-4	0.0	0.0	21.146058825359713	0.0
34	2.222508678896391E-4	0.0	0.0	21.36475367936312	0.0
35	2.222508678896391E-4	0.0	0.0	21.60256210800503	0.0
36	2.222508678896391E-4	0.0	0.0	21.82592423023412	0.0
37	4.445017357792782E-4	0.0	0.0	22.06684417102649	0.0
38	4.445017357792782E-4	0.0	0.0	22.303096843593174	0.0
39	4.445017357792782E-4	0.0	0.0	22.545350289592882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATACG	45	3.8380676E-10	45.000004	17
TACGCGG	20	7.0287054E-4	45.0	2
CTACGGG	30	2.1625456E-6	44.999996	3
CGTTTTT	11110	0.0	43.784874	1
TTGCTTG	305	0.0	40.57377	1
GTCGTTT	50	1.0786607E-9	40.5	9
CCCATTA	45	1.9237632E-8	40.000004	33
TCGTTTG	85	0.0	39.705883	1
GGCGATA	40	3.451969E-7	39.375	8
GTTTTTT	12625	0.0	39.297028	2
TATGCGG	35	6.239814E-6	38.57143	2
AGACACG	105	0.0	38.57143	24
TCAAGCG	105	0.0	38.57143	17
TTTGGGC	1085	0.0	38.15668	4
TGGGCGA	645	0.0	38.023254	6
ACACGTG	90	0.0	37.500004	42
ACGTGAG	90	0.0	37.500004	44
CACGTGA	90	0.0	37.500004	43
TGGGTAT	330	0.0	37.5	6
TGCGGGA	365	0.0	36.986298	4
>>END_MODULE