FastQCFastQC Report
Sat 14 Jan 2017
SRR2933585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455277
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216904.764132604985536No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC8750.19219068830623995No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT5550.12190380801138648TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC4740.10411244143675169No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC4680.10279456243122319TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4560.1001588044201662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG207.0287916E-445.0000042
CAACTCG207.0287916E-445.00000433
AACCCGA351.2097735E-745.024
CGTAACT253.8870814E-545.015
CGCGTAA253.8870814E-545.013
TCGCGTA253.8870814E-545.012
TGCGTTG502.1827873E-1145.01
CTACGAA302.1626038E-644.99999611
TACGAAT302.1626038E-644.99999612
CGAATAT302.1626038E-644.99999614
GTACGGG302.1626038E-644.9999963
CGTTTTT110050.043.9981841
GGCACCG1350.041.6666648
TCAAGCG1350.041.66666417
GACACGA1300.041.5384625
CACGACC1300.041.5384627
AGACACG1300.041.5384624
CGGGTCT603.6379788E-1241.2499966
TCGTTTG1000.040.51
GTACGGC501.0786607E-940.59
Produced by FastQC (version 0.11.5)