FastQCFastQC Report
Sat 14 Jan 2017
SRR2933584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83740
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35884.284690709338428No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC4230.5051349414855505No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC3890.46453307857654647No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT3190.3809410078815381No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC2860.34153331741103415No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC2480.2961547647480296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.2770480057320277No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC2120.25316455696202533No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.22569859087652258No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG1150.13732983042751373No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.13135896823501314No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGCTACCATCGT980.1170288989730117No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG920.10986386434201098No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG870.10389300214951039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCC351.1939665E-745.00000411
CGGGTAT206.995823E-445.06
TGCCTCA206.995823E-445.022
AGTGCCT206.995823E-445.020
CGGGAGC206.995823E-445.06
CGGGACC206.995823E-445.06
TCGTTTG206.995823E-445.01
GTCTAGG253.859786E-545.07
TGGGTGC253.859786E-545.06
CAGTGCC206.995823E-445.019
TTCGGGC253.859786E-545.04
GTGCCTC206.995823E-445.021
GGGCCAC206.995823E-445.07
GGACTAA302.1413853E-644.9999968
TCTGTTG302.1413853E-644.9999961
GTTCGGG302.1413853E-644.9999963
GACTAAT302.1413853E-644.9999969
CGTTTTT12850.043.4241261
TGGGACC700.041.7857176
GTTGGGA650.041.538464
Produced by FastQC (version 0.11.5)