##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84541
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910422162027892	33.0	31.0	34.0	30.0	34.0
2	32.10411516305698	34.0	31.0	34.0	30.0	34.0
3	32.234028459564	34.0	31.0	34.0	30.0	34.0
4	35.79630002010859	37.0	35.0	37.0	35.0	37.0
5	35.85410629162182	37.0	35.0	37.0	35.0	37.0
6	35.83464827716729	37.0	35.0	37.0	35.0	37.0
7	35.790196472717376	37.0	35.0	37.0	35.0	37.0
8	35.64113270484144	37.0	35.0	37.0	33.0	37.0
9	37.48294910161933	39.0	37.0	39.0	34.0	39.0
10	37.06983593759241	39.0	37.0	39.0	33.0	39.0
11	37.05748689984741	39.0	37.0	39.0	33.0	39.0
12	37.02040430087177	39.0	37.0	39.0	33.0	39.0
13	37.001324800984136	39.0	37.0	39.0	33.0	39.0
14	38.102482818987234	40.0	37.0	41.0	33.0	41.0
15	38.12863580984374	40.0	37.0	41.0	33.0	41.0
16	38.257661962834604	40.0	38.0	41.0	33.0	41.0
17	38.2652085970121	40.0	38.0	41.0	33.0	41.0
18	38.06458404797672	39.0	37.0	41.0	34.0	41.0
19	37.79766030683337	39.0	37.0	41.0	34.0	41.0
20	37.36320838409765	39.0	35.0	40.0	33.0	41.0
21	37.2783974639524	39.0	35.0	40.0	33.0	41.0
22	37.25882116369572	39.0	35.0	40.0	33.0	41.0
23	37.13799221679422	39.0	35.0	40.0	33.0	41.0
24	37.03778048520836	39.0	35.0	40.0	33.0	41.0
25	36.93572349510888	38.0	35.0	40.0	33.0	41.0
26	36.900154954400826	38.0	35.0	40.0	33.0	41.0
27	36.85248577613229	38.0	35.0	40.0	33.0	41.0
28	36.777445263245056	38.0	35.0	40.0	33.0	41.0
29	36.60030044593747	38.0	35.0	40.0	32.0	41.0
30	36.383364284784896	38.0	35.0	40.0	31.0	41.0
31	36.088099265445166	38.0	35.0	40.0	30.0	41.0
32	35.78500372600277	38.0	35.0	40.0	30.0	41.0
33	35.325037555742185	38.0	35.0	40.0	25.0	41.0
34	34.99095113613513	38.0	35.0	40.0	23.0	41.0
35	34.71907121988148	38.0	35.0	40.0	21.0	41.0
36	34.53642611277368	38.0	34.0	40.0	20.0	41.0
37	34.432961521628556	38.0	34.0	40.0	18.0	41.0
38	34.315255319903955	38.0	34.0	40.0	18.0	41.0
39	34.27253048816551	38.0	34.0	40.0	18.0	41.0
40	34.082303261139565	38.0	34.0	40.0	18.0	41.0
41	33.946534817425864	38.0	33.0	40.0	17.0	41.0
42	33.719733620373546	38.0	33.0	40.0	15.0	41.0
43	33.59724867224187	37.0	33.0	40.0	15.0	41.0
44	33.46854189091683	37.0	33.0	40.0	15.0	41.0
45	33.48123395748808	37.0	33.0	40.0	15.0	41.0
46	33.23547154635029	37.0	33.0	40.0	15.0	41.0
47	33.13173489786021	37.0	33.0	40.0	15.0	41.0
48	33.05494375510108	37.0	33.0	40.0	14.0	41.0
49	33.075738399119956	37.0	33.0	40.0	13.0	41.0
50	32.89600312274518	36.0	32.0	40.0	12.0	41.0
51	31.953076022285046	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	2.0
12	6.0
13	5.0
14	6.0
15	10.0
16	17.0
17	24.0
18	42.0
19	81.0
20	119.0
21	208.0
22	281.0
23	453.0
24	730.0
25	1382.0
26	1929.0
27	2268.0
28	2152.0
29	2035.0
30	2062.0
31	2253.0
32	2577.0
33	3302.0
34	5019.0
35	6638.0
36	8468.0
37	10286.0
38	16734.0
39	15445.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.077560000473145	5.895364379413539	39.979418270425	31.04765734968832
2	34.476762754166614	5.892998663370435	40.73408168817497	18.89615689428798
3	19.722974651352597	5.877621509090264	54.46824617641145	19.931157663145694
4	15.295537076684685	5.833855762292852	56.169196011402754	22.70141114961971
5	13.591038667628725	7.188228196969518	57.00074520055358	22.219987934848177
6	17.915567594421642	8.148708910469477	60.12703895151464	13.808684543594232
7	64.29069918737655	3.802888539288629	26.558711157899715	5.347701115435114
8	62.78492092594126	3.9862315326291387	26.584734034373852	6.6441135070557475
9	57.771968630605265	6.059781644409221	28.71269561514531	7.455554109840196
10	29.579730544942688	20.251712186986197	34.66365432157178	15.504902946499332
11	20.321500810257746	18.80625968465005	39.13722335908021	21.735016146011993
12	17.8138418045682	16.263114938313954	45.52347381743769	20.399569439680153
13	20.41021516187412	17.230692799943224	46.40115446942904	15.957937568753621
14	18.32483646987852	19.486403047042263	44.993553423782544	17.19520705929667
15	13.742444494387337	17.877716137731987	47.32141801019624	21.058421357684438
16	14.207307696857146	17.807927514460438	44.70966749861014	23.27509729007227
17	14.143433363693356	18.294082161318176	42.86204326894642	24.70044120604204
18	15.436297181249333	17.507481576986315	45.52229095941614	21.53393028234821
19	16.04783477839155	19.615334571391397	45.19937071953253	19.13745993068452
20	16.265480654357056	22.811416945624018	42.98860907725246	17.934493322766468
21	15.776960291456216	23.795554819555008	42.79816893578264	17.629315953206138
22	14.26645059793473	22.061484959960257	41.25099064359305	22.421073798511966
23	14.411942134585587	22.49322813782662	39.10646905051986	23.98836067706793
24	15.028211163814007	22.27676511988266	39.712092357554326	22.98293135874901
25	14.445062159189032	24.42601814504205	37.34282774038632	23.786091955382595
26	13.910410333447675	25.068310050744607	38.717308761429365	22.30397085437835
27	15.877503223288109	23.671354727292083	39.979418270425	20.471723778994807
28	13.781478809098543	21.934919151654228	40.08942406642931	24.194177972817922
29	15.250588471865722	21.54339314652062	39.639938018239675	23.566080363373985
30	20.604203877408594	22.78539406914988	36.61418719911049	19.996214854331036
31	19.446185874309506	26.316225263481623	31.796406477330525	22.441182384878346
32	17.029606936279436	26.747968441347986	31.747909298446906	24.47451532392567
33	19.73953466365432	23.62640612247312	30.777965720774535	25.856093493098026
34	18.01965910031819	22.966371346447286	31.62134349014088	27.392626063093644
35	17.519310157201833	26.320956695567833	30.146319537265942	26.0134136099644
36	21.682970393063723	28.92915863308927	27.747483469559146	21.64038750428786
37	20.33214653245171	24.373972392093776	29.91684508108492	25.377035994369596
38	17.288652842999255	24.959487112761856	31.557469156977085	26.194390887261804
39	17.923847600572504	26.753882731455743	31.842537940171038	23.47973172780071
40	19.20843141197762	25.526076105085103	29.666079180515965	25.599413302421308
41	17.352527176163047	25.361658840089422	26.887545687891084	30.39826829585645
42	18.900888326374186	26.617854058977304	28.61688411539963	25.864373499248885
43	19.382311541145718	24.345583799576538	33.493807738257175	22.77829692102057
44	18.547213777930235	22.823245525839532	32.89055014726582	25.73899054896441
45	21.144769993257707	20.89755266675341	31.163577435800384	26.7940999041885
46	22.280313693947313	21.30209011012408	33.03959025798133	23.378005937947268
47	16.37193787629671	25.1688529825765	35.213683301593306	23.24552583953348
48	16.53872085733549	24.36805810198602	35.939958126826035	23.15326291385245
49	20.252895045007747	20.755609704167206	36.75376444565359	22.237730805171456
50	20.35225511881809	18.396990809193174	33.90425947173561	27.34649460025313
51	18.291716445275075	18.326019327900074	31.756189304597772	31.626074922227087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	334.0
1	537.5
2	741.0
3	3033.5
4	5326.0
5	3379.5
6	1433.0
7	1346.0
8	1259.0
9	1256.0
10	1253.0
11	1177.0
12	1101.0
13	1051.5
14	1002.0
15	910.0
16	818.0
17	782.5
18	747.0
19	677.5
20	608.0
21	592.5
22	577.0
23	544.0
24	511.0
25	512.0
26	543.5
27	574.0
28	545.0
29	516.0
30	601.0
31	686.0
32	776.5
33	867.0
34	958.5
35	1050.0
36	1127.0
37	1204.0
38	1355.0
39	1506.0
40	1899.5
41	2293.0
42	2772.0
43	3251.0
44	3819.5
45	4388.0
46	5595.0
47	6802.0
48	8435.0
49	10068.0
50	10161.5
51	10255.0
52	8677.0
53	7099.0
54	5628.5
55	4158.0
56	3434.5
57	2711.0
58	2505.5
59	2300.0
60	2114.5
61	1929.0
62	1761.0
63	1593.0
64	1524.0
65	1455.0
66	1254.0
67	1053.0
68	963.0
69	873.0
70	745.5
71	618.0
72	514.0
73	410.0
74	335.0
75	226.0
76	192.0
77	147.0
78	102.0
79	80.5
80	59.0
81	41.5
82	24.0
83	18.5
84	13.0
85	8.0
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	84541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.70400160868691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65129233511587	50.74106055050213
2	11.85940285204991	15.109828367301073
3	4.296642899584076	8.211400385611716
4	1.7899584076054667	4.561100531103252
5	0.8467023172905526	2.6969162891378144
6	0.41406714200831846	1.5826640328361385
7	0.22838680926916222	1.0184407565559905
8	0.19867795603089722	1.012526466448232
9	0.12254901960784313	0.7026176648016939
>10	0.5440433749257279	6.1023645331850815
>50	0.027852049910873443	1.1887723116594315
>100	0.018568033273915627	2.952413621792976
>500	0.0	0.0
>1k	0.0018568033273915628	4.119894489064477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3483	4.119894489064477	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	435	0.5145432393749778	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	345	0.40808601743532724	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	331	0.3915260051336038	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	310	0.36668598668101865	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	274	0.32410309790515845	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	216	0.2554973326551614	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	194	0.2294744561810246	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	172	0.20345157970688776	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	111	0.13129724039223573	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCAGCTACCATCGT	108	0.1277486663275807	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.1052743639180989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43647460995256737	0.0
2	0.0	0.0	0.0	1.6181497734826888	0.0
3	0.0	0.0	0.0	2.272270259400764	0.0
4	0.0	0.0	0.0	3.1688766397369323	0.0
5	0.0	0.0	0.0	5.085106634650643	0.0
6	0.0	0.0	0.0	6.595616328172129	0.0
7	0.0	0.0	0.0	8.097846015542755	0.0
8	0.0	0.0	0.0	10.242367608615938	0.0
9	0.0	0.0	0.0	11.344791284702097	0.0
10	0.0	0.0	0.0	13.568564365219244	0.0
11	0.0	0.0	0.0	18.801528252563845	0.0
12	0.0	0.0	0.0	23.56253178930933	0.0
13	0.0	0.0	0.0	25.08368720502478	0.0
14	0.0	0.0	0.0	25.665653351628205	0.0
15	0.0	0.0	0.0	26.456985368046272	0.0
16	0.0	0.0	0.0	28.38622680119705	0.0
17	0.0	0.0	0.0	30.72591996782626	0.0
18	0.0	0.0	0.0	32.75452147478738	0.0
19	0.0	0.0	0.0	33.972865236985605	0.0
20	0.0	0.0	0.0	35.038620314403666	0.0
21	0.0	0.0	0.0	36.33621556404585	0.0
22	0.0	0.0	0.0	37.30970771578288	0.0
23	0.0	0.0	0.0	38.089211151985424	0.0
24	0.0	0.0	0.0	38.59784010125264	0.0
25	0.0	0.0	0.0	38.90420032883453	0.0
26	0.0	0.0	0.0	39.14077193314486	0.0
27	0.0	0.0	0.0	39.37024638932589	0.0
28	0.0	0.0	0.0	39.521652216084505	0.0
29	0.0	0.0	0.0	39.702629493381906	0.0
30	0.0	0.0	0.0	39.885972486722416	0.0
31	0.0	0.0	0.0	40.05748689984741	0.0
32	0.0	0.0	0.0	40.245561325274124	0.0
33	0.0	0.0	0.0	40.400515726097396	0.0
34	0.0	0.0	0.0	40.53891011461894	0.0
35	0.0	0.0	0.0	40.70332737961463	0.0
36	0.0	0.0	0.0	40.81806460770514	0.0
37	0.0	0.0	0.0	40.95645899622668	0.0
38	0.0	0.0	0.0	41.11851054517926	0.0
39	0.0	0.0	0.0	41.31368211873529	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCAAC	20	6.996206E-4	45.0	7
GCGATCT	20	6.996206E-4	45.0	9
CAACCCG	20	6.996206E-4	45.0	23
AGGGTGG	20	6.996206E-4	45.0	6
AACCCGA	20	6.996206E-4	45.0	24
CATTCCC	20	6.996206E-4	45.0	13
ACCCGAG	20	6.996206E-4	45.0	41
CTAGGGT	20	6.996206E-4	45.0	4
ACCTGGG	20	6.996206E-4	45.0	3
CGGGATC	20	6.996206E-4	45.0	6
CGCGGGA	20	6.996206E-4	45.0	4
TGGGCAA	20	6.996206E-4	45.0	6
TGGGTAC	20	6.996206E-4	45.0	6
AGACCCG	20	6.996206E-4	45.0	39
TGGGACA	20	6.996206E-4	45.0	6
CCAAGCA	20	6.996206E-4	45.0	18
CGACTCC	20	6.996206E-4	45.0	28
TGCGGGT	20	6.996206E-4	45.0	4
TTGCCTG	20	6.996206E-4	45.0	1
GGCGATC	25	3.860103E-5	44.999996	8
>>END_MODULE