FastQCFastQC Report
Sat 14 Jan 2017
SRR2933582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064115
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521244.89834275430757No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC12970.12188532254502568No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA12320.11577696019697119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG302.1653486E-645.0000041
ACCGGTA351.2118107E-745.041
ACGGGTA207.033052E-445.05
CGTTTTT367500.044.4367371
CGAATAT800.042.187514
GTTTTTT411000.040.4069332
GCCGATT1700.039.7058839
TACGAAT850.039.70588312
TACCGGT403.4578989E-739.37540
GGCACCG3250.038.769238
ACGGGCC356.247854E-638.5714265
CGAACTA301.13986476E-437.50000443
CGGATAG301.13986476E-437.50000436
CTACGAA900.037.511
TCGTTTG2350.037.3404271
GCGATCT2600.037.2115369
CGACCAA1400.036.96428329
AACACGT1350.036.66666841
TGGGCGA16450.036.6565366
TTGGGCA15850.036.3406945
Produced by FastQC (version 0.11.5)