FastQCFastQC Report
Sat 14 Jan 2017
SRR2933581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074225
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513434.779538737229165No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC12880.11990039330680258No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA12570.11701459191510158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT366600.044.3555641
GCCGATG650.041.5384649
GTTTTTT412850.040.0405732
CTTTGCG2850.037.8947371
TCCGATA301.1398723E-437.49999614
TACGGGT601.546141E-1037.4999964
TAATGCG301.1398723E-437.49999624
ACGAACG301.1398723E-437.49999621
TGGGCGA17250.036.7826086
TTGGGCG14050.036.0320245
TTGGGAC14300.035.5594375
GGCCGAT3000.035.258
GGGCGAT25800.035.2325557
TCAAGCG1150.035.2173917
GGCGATT13250.035.1509448
TGCGGGC5900.035.0847474
CTAAACG458.699226E-735.00000445
GCCGATT1550.034.8387079
CGTTTGG5300.034.811322
TTGGGTC9650.034.7409325
Produced by FastQC (version 0.11.5)