##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1074225
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53678977867765	34.0	31.0	34.0	31.0	34.0
2	32.75838860573902	34.0	31.0	34.0	31.0	34.0
3	32.76610114268426	34.0	31.0	34.0	31.0	34.0
4	36.22432544392469	37.0	37.0	37.0	35.0	37.0
5	36.27862319346506	37.0	37.0	37.0	35.0	37.0
6	36.286472573250485	37.0	37.0	37.0	35.0	37.0
7	36.28611882985408	37.0	37.0	37.0	35.0	37.0
8	36.240119155670364	37.0	37.0	37.0	35.0	37.0
9	38.10282761991203	39.0	39.0	39.0	37.0	39.0
10	37.56760082850427	39.0	38.0	39.0	35.0	39.0
11	37.53743582582792	39.0	37.0	39.0	35.0	39.0
12	37.553627033442716	39.0	37.0	39.0	35.0	39.0
13	37.606176545881915	39.0	37.0	39.0	35.0	39.0
14	39.06962414764132	40.0	39.0	41.0	35.0	41.0
15	39.096739509879214	41.0	39.0	41.0	35.0	41.0
16	39.14131676324792	41.0	39.0	41.0	35.0	41.0
17	39.09576857734646	41.0	39.0	41.0	35.0	41.0
18	38.50131210872955	39.0	38.0	40.0	35.0	41.0
19	37.88185436012009	38.0	37.0	40.0	35.0	41.0
20	37.13118294584468	38.0	35.0	40.0	34.0	41.0
21	37.06646093695455	38.0	35.0	40.0	34.0	41.0
22	37.02895110428448	37.0	35.0	40.0	34.0	41.0
23	36.90156345272173	37.0	35.0	40.0	34.0	41.0
24	36.789356047382995	37.0	35.0	40.0	33.0	41.0
25	36.731780586003865	37.0	35.0	40.0	33.0	41.0
26	36.698066047615725	37.0	35.0	40.0	33.0	41.0
27	36.610985594265635	37.0	35.0	40.0	33.0	41.0
28	36.481158975075054	37.0	35.0	40.0	33.0	41.0
29	36.21249365821872	37.0	35.0	40.0	33.0	41.0
30	35.79924224440876	36.0	35.0	40.0	31.0	41.0
31	35.156863785519796	36.0	35.0	40.0	27.0	41.0
32	34.223224184877466	36.0	34.0	40.0	20.0	41.0
33	32.89891316995974	36.0	33.0	40.0	12.0	41.0
34	31.7986436733459	36.0	30.0	40.0	8.0	41.0
35	31.105934045474644	36.0	24.0	40.0	7.0	41.0
36	30.681993064767624	36.0	23.0	40.0	7.0	41.0
37	30.451243454583537	36.0	22.0	40.0	7.0	41.0
38	30.30710884591217	36.0	21.0	40.0	7.0	41.0
39	30.22524238404431	36.0	21.0	40.0	7.0	41.0
40	30.071353766668995	35.0	20.0	40.0	7.0	41.0
41	29.902394749703273	35.0	18.0	40.0	7.0	41.0
42	29.69461611859713	35.0	18.0	40.0	7.0	41.0
43	29.56843864181154	35.0	18.0	40.0	7.0	41.0
44	29.394871651655844	35.0	18.0	40.0	7.0	41.0
45	29.34598245246573	35.0	18.0	40.0	7.0	41.0
46	29.2152621657474	35.0	18.0	40.0	7.0	41.0
47	29.18039330680258	35.0	18.0	40.0	7.0	41.0
48	29.047552421513185	35.0	18.0	40.0	7.0	41.0
49	28.995645232609554	35.0	18.0	40.0	7.0	41.0
50	28.843033815075984	35.0	18.0	40.0	7.0	41.0
51	28.20055109497545	35.0	17.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	7.0
11	17.0
12	22.0
13	21.0
14	30.0
15	36.0
16	79.0
17	205.0
18	487.0
19	1007.0
20	1772.0
21	2872.0
22	4882.0
23	8968.0
24	18979.0
25	41590.0
26	66489.0
27	68872.0
28	52488.0
29	37834.0
30	29245.0
31	26047.0
32	25671.0
33	30120.0
34	44277.0
35	59153.0
36	73076.0
37	90639.0
38	165133.0
39	224116.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.344876538900138	3.218506365053876	34.598245246573114	44.83837184947287
2	49.0253903977286	3.2418720472899065	36.57483301915334	11.157904535828154
3	14.75687123274919	3.334403872559287	69.24266331541344	12.666061579278084
4	11.977192859968815	3.3909097256161416	69.84318927598967	14.788708138425378
5	11.248388373013102	4.062370546207731	69.8419791012125	14.847261979566664
6	13.559356745560752	4.971956526798389	70.95068537783052	10.518001349810328
7	56.80616258232679	1.4444832320975587	39.37275710395867	2.3765970816169797
8	58.96129767972259	1.4561195280318369	37.75642905350369	1.8261537387418838
9	55.337941306523305	3.228560124741092	38.78507761409388	2.6484209546417183
10	25.509739579696987	15.244292396844235	46.52796201913007	12.718006004328702
11	18.794665921943725	17.787148874770185	49.2832507156322	14.134934487653892
12	14.235006632688682	16.498219646722056	54.69533849984873	14.571435220740533
13	14.503665433219298	16.20247154925644	56.399078405362005	12.894784612162258
14	14.785543065931254	16.973632153412925	55.674835346412536	12.56598943424329
15	13.90760781028183	17.019246433475296	55.74223277246387	13.330912983779003
16	14.958784239800787	16.34033838348577	54.459540599036515	14.24133677767693
17	15.544508831948614	16.75868649491494	53.25653378016709	14.44027089296935
18	16.148386045753917	16.185063650538762	53.4248411645605	14.241709139146828
19	16.38064651260211	18.019130070515953	51.714584933324026	13.885638483557914
20	16.962833670785916	17.46812818543601	51.813539993949135	13.755498149828945
21	17.336219134725035	18.12218110730992	51.51900207126068	13.022597686704367
22	16.844701994461122	16.624357094649632	51.808513114105516	14.722427796783727
23	15.79492192045428	18.1219018362075	50.92843678000419	15.154739463334032
24	15.042844841630012	18.45088319486141	51.93008913402686	14.576182829481718
25	15.135190486164444	19.10670483371733	51.10223649607857	14.655868184039655
26	15.239730968838	19.055598221973984	51.209569689776345	14.495101119411668
27	14.983639367916405	18.715166748120737	51.561265098093976	14.739928785868884
28	14.960087504945427	18.36626405082734	51.62568363238614	15.047964811841094
29	15.805627312713815	17.81786869603668	51.04759245037119	15.328911540878307
30	16.57762573017757	18.178221508529404	50.30761711931858	14.936535641974446
31	16.49700947194489	19.67027391840629	48.67155391095906	15.161162698689754
32	16.329912262328655	19.975703414089228	47.44937047638996	16.245013847192162
33	18.265167911750332	20.696688310177105	43.78151690753799	17.25662687053457
34	18.040820126137447	22.560636738113523	40.938816355977565	18.45972677977146
35	18.916893574437385	23.602224859782634	38.75566105797203	18.725220507807954
36	19.940980707021343	25.10740301147339	36.63199050478252	18.319625776722752
37	19.452907910353975	26.615746235658268	36.42989131699597	17.501454536991783
38	20.07847517978077	26.666666666666668	35.5402266750448	17.714631478507762
39	20.354581209709323	25.79897135143941	35.366799320440315	18.479648118410946
40	20.268379529428195	26.047801903698016	35.20147082780609	18.482347739067702
41	19.077195187228003	26.17878005073425	35.413716865647324	19.330307896390423
42	19.374060369103308	26.908701622099652	35.38597593614001	18.331262072657033
43	19.63694756685052	26.16975028508925	35.70899951127557	18.48430263678466
44	20.013684284018712	25.779701645372242	35.2906513998464	18.915962670762642
45	20.01210174777165	25.694430868765856	34.73415718308548	19.559310200377016
46	20.127161441969793	25.767692987968072	34.72512741744048	19.38001815262166
47	19.07542647024599	26.524890037003424	35.637226837952944	18.762456654797642
48	19.38783774348949	25.780632549046988	35.78030673276083	19.051222974702693
49	19.42125718541274	25.626940352347038	35.618888035560516	19.332914426679697
50	19.147292233936096	25.108427005515605	35.537713235123	20.206567525425307
51	18.92555097861249	24.89906676906607	34.968837999488	21.20654425283344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	728.0
1	1834.5
2	2941.0
3	44353.5
4	85766.0
5	58090.0
6	30414.0
7	29826.5
8	29239.0
9	29750.0
10	30261.0
11	29817.5
12	29374.0
13	28634.5
14	27895.0
15	26974.5
16	26054.0
17	24915.0
18	23776.0
19	22520.5
20	21265.0
21	20545.0
22	19825.0
23	18837.5
24	17850.0
25	17291.5
26	16362.0
27	15991.0
28	15856.5
29	15722.0
30	16769.5
31	17817.0
32	19110.0
33	20403.0
34	23036.5
35	25670.0
36	28177.5
37	30685.0
38	32677.0
39	34669.0
40	36742.5
41	38816.0
42	41038.0
43	43260.0
44	45685.5
45	48111.0
46	51202.0
47	54293.0
48	56831.0
49	59369.0
50	58157.5
51	56946.0
52	53382.5
53	49819.0
54	45853.5
55	41888.0
56	39032.5
57	36177.0
58	33878.5
59	31580.0
60	28559.0
61	25538.0
62	22532.0
63	19526.0
64	17182.0
65	14838.0
66	12648.5
67	10459.0
68	8781.0
69	7103.0
70	6078.0
71	5053.0
72	4168.0
73	3283.0
74	2776.5
75	1805.0
76	1340.0
77	1026.5
78	713.0
79	568.0
80	423.0
81	317.5
82	212.0
83	147.5
84	83.0
85	57.0
86	31.0
87	21.0
88	11.0
89	8.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1074225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.546187100019054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5253385175908	34.980044216969326
2	7.264094296543441	6.471754072920108
3	3.4331482607890513	4.588009943016423
4	2.1963081019548167	3.913486065558679
5	1.5179089046556036	3.3808527033786744
6	1.2066033422551412	3.2249746943763515
7	0.9182696752146144	2.863378893226876
8	0.7439266377765325	2.651127615606521
9	0.5993633439205038	2.402941649325825
>10	3.49542777838707	25.17602732439786
>50	0.061918060757287105	1.8807911746886425
>100	0.03474501812165217	2.652388291056883
>500	0.0021057585953312796	0.6593449166005813
>1k	6.317275785993838E-4	0.33872449763048784
>5k	0.0	0.0
>10k+	2.1057585953312798E-4	4.816153941246784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51343	4.779538737229165	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC	1288	0.11990039330680258	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1257	0.11701459191510158	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.861807349484512E-4	0.0	0.0	0.04933789476133957	0.0
2	1.861807349484512E-4	0.0	0.0	0.15108566641066815	0.0
3	1.861807349484512E-4	0.0	0.0	0.2465032930717494	0.0
4	1.861807349484512E-4	0.0	0.0	0.36854476483045917	0.0
5	1.861807349484512E-4	0.0	0.0	0.6532151085666411	0.0
6	1.861807349484512E-4	0.0	0.0	1.1096371802927691	0.0
7	1.861807349484512E-4	0.0	0.0	1.4759477763038469	0.0
8	1.861807349484512E-4	0.0	0.0	2.022295143010077	0.0
9	1.861807349484512E-4	0.0	0.0	2.337592217645279	0.0
10	1.861807349484512E-4	0.0	0.0	2.7858223370336757	0.0
11	1.861807349484512E-4	0.0	0.0	3.523842770369336	0.0
12	1.861807349484512E-4	0.0	0.0	4.140101003048709	0.0
13	1.861807349484512E-4	0.0	0.0	4.401033303078964	0.0
14	1.861807349484512E-4	0.0	0.0	4.511624659638344	0.0
15	1.861807349484512E-4	0.0	0.0	4.62081966068561	0.0
16	1.861807349484512E-4	0.0	0.0	4.834275873304009	0.0
17	1.861807349484512E-4	1.861807349484512E-4	0.0	5.123414554678955	0.0
18	1.861807349484512E-4	1.861807349484512E-4	0.0	5.414228862668435	0.0
19	1.861807349484512E-4	1.861807349484512E-4	0.0	5.597709976960134	0.0
20	1.861807349484512E-4	1.861807349484512E-4	0.0	5.77123042193209	0.0
21	1.861807349484512E-4	1.861807349484512E-4	0.0	5.9774255858875005	0.0
22	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.1697502850892505	0.0
23	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.342060555284042	0.0
24	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.469966720193628	5.585422048453537E-4
25	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.586236589168935	5.585422048453537E-4
26	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.698131210872955	5.585422048453537E-4
27	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.800437524727129	5.585422048453537E-4
28	2.7927110242267683E-4	1.861807349484512E-4	0.0	6.914752495985478	5.585422048453537E-4
29	2.7927110242267683E-4	1.861807349484512E-4	0.0	7.027298750261816	5.585422048453537E-4
30	2.7927110242267683E-4	1.861807349484512E-4	0.0	7.148781679815681	5.585422048453537E-4
31	2.7927110242267683E-4	1.861807349484512E-4	0.0	7.265703181363309	5.585422048453537E-4
32	3.723614698969024E-4	1.861807349484512E-4	0.0	7.395191882519956	5.585422048453537E-4
33	3.723614698969024E-4	1.861807349484512E-4	0.0	7.522074053387326	5.585422048453537E-4
34	4.6545183737112803E-4	1.861807349484512E-4	0.0	7.6501663990318605	5.585422048453537E-4
35	4.6545183737112803E-4	1.861807349484512E-4	0.0	7.782540901580209	5.585422048453537E-4
36	4.6545183737112803E-4	1.861807349484512E-4	0.0	7.916963392212991	5.585422048453537E-4
37	4.6545183737112803E-4	1.861807349484512E-4	0.0	8.060694919593194	5.585422048453537E-4
38	4.6545183737112803E-4	1.861807349484512E-4	0.0	8.205450441015616	5.585422048453537E-4
39	4.6545183737112803E-4	1.861807349484512E-4	0.0	8.361562987269892	5.585422048453537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36660	0.0	44.355564	1
GCCGATG	65	0.0	41.538464	9
GTTTTTT	41285	0.0	40.040573	2
CTTTGCG	285	0.0	37.894737	1
TCCGATA	30	1.1398723E-4	37.499996	14
TACGGGT	60	1.546141E-10	37.499996	4
TAATGCG	30	1.1398723E-4	37.499996	24
ACGAACG	30	1.1398723E-4	37.499996	21
TGGGCGA	1725	0.0	36.782608	6
TTGGGCG	1405	0.0	36.032024	5
TTGGGAC	1430	0.0	35.559437	5
GGCCGAT	300	0.0	35.25	8
GGGCGAT	2580	0.0	35.232555	7
TCAAGCG	115	0.0	35.21739	17
GGCGATT	1325	0.0	35.150944	8
TGCGGGC	590	0.0	35.084747	4
CTAAACG	45	8.699226E-7	35.000004	45
GCCGATT	155	0.0	34.838707	9
CGTTTGG	530	0.0	34.81132	2
TTGGGTC	965	0.0	34.740932	5
>>END_MODULE