##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933578.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145220
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.901618234402974	33.0	31.0	34.0	30.0	34.0
2	32.11815176972869	33.0	31.0	34.0	30.0	34.0
3	32.19713538080154	33.0	31.0	34.0	30.0	34.0
4	35.846219528990495	37.0	35.0	37.0	35.0	37.0
5	35.87271037047239	37.0	35.0	37.0	35.0	37.0
6	35.85629389891199	37.0	35.0	37.0	35.0	37.0
7	35.90253408621402	37.0	35.0	37.0	35.0	37.0
8	35.71765597025203	37.0	35.0	37.0	35.0	37.0
9	37.52948629665335	39.0	37.0	39.0	35.0	39.0
10	37.04092411513566	39.0	37.0	39.0	33.0	39.0
11	37.10172841206445	39.0	37.0	39.0	33.0	39.0
12	37.13009227379149	39.0	37.0	39.0	34.0	39.0
13	37.02224900151494	39.0	37.0	39.0	33.0	39.0
14	38.00208648946426	40.0	37.0	41.0	33.0	41.0
15	38.05979203966396	40.0	37.0	41.0	33.0	41.0
16	38.15824266629941	40.0	37.0	41.0	33.0	41.0
17	38.16561079741083	40.0	37.0	41.0	33.0	41.0
18	37.79959371987329	39.0	37.0	40.0	33.0	41.0
19	37.3701005371161	38.0	37.0	40.0	33.0	41.0
20	36.75391130698251	38.0	35.0	40.0	33.0	41.0
21	36.61613414130285	37.0	35.0	40.0	32.0	41.0
22	36.66728412064454	38.0	35.0	40.0	33.0	41.0
23	36.63300509571685	38.0	35.0	40.0	33.0	41.0
24	36.49697700041317	37.0	35.0	40.0	32.0	41.0
25	36.38892714502135	37.0	35.0	40.0	32.0	41.0
26	36.39442225588762	37.0	35.0	40.0	32.0	41.0
27	36.32400495799477	37.0	35.0	40.0	32.0	41.0
28	36.194787219391266	37.0	35.0	40.0	32.0	41.0
29	36.11407519625396	37.0	35.0	40.0	31.0	41.0
30	35.95495110866272	37.0	35.0	40.0	31.0	41.0
31	35.567504475967496	37.0	35.0	40.0	30.0	41.0
32	35.11462608456136	37.0	35.0	40.0	27.0	41.0
33	34.57783363173117	37.0	34.0	40.0	22.0	41.0
34	34.08381765597025	38.0	34.0	40.0	18.0	41.0
35	33.67758573199284	37.0	33.0	40.0	15.0	41.0
36	33.42682137446633	37.0	33.0	40.0	15.0	41.0
37	33.21585869714915	37.0	33.0	40.0	12.0	41.0
38	33.10730615617683	37.0	33.0	40.0	12.0	41.0
39	33.0886448147638	37.0	33.0	40.0	10.0	41.0
40	32.930250654179865	37.0	33.0	40.0	10.0	41.0
41	32.709674975898636	37.0	32.0	40.0	10.0	41.0
42	32.520926869577195	37.0	31.0	40.0	10.0	41.0
43	32.32164991048065	36.0	31.0	40.0	10.0	41.0
44	32.21479135105358	36.0	31.0	40.0	10.0	41.0
45	32.20249965569481	36.0	31.0	40.0	10.0	41.0
46	31.89504200523344	36.0	31.0	40.0	10.0	41.0
47	31.667139512463848	35.0	30.0	40.0	10.0	41.0
48	31.61016388927145	35.0	29.0	39.0	8.0	41.0
49	31.674259743836938	35.0	30.0	39.0	8.0	41.0
50	31.573991185787083	35.0	30.0	39.0	8.0	41.0
51	30.499373364550337	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	8.0
13	3.0
14	2.0
15	5.0
16	10.0
17	31.0
18	73.0
19	118.0
20	219.0
21	324.0
22	564.0
23	848.0
24	1576.0
25	2953.0
26	4483.0
27	5027.0
28	4612.0
29	4385.0
30	4310.0
31	4670.0
32	5095.0
33	6178.0
34	8809.0
35	12499.0
36	15696.0
37	17109.0
38	26724.0
39	18884.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.791213331497037	4.364412615342239	37.833631731166506	37.010742321994215
2	42.74411238121471	4.498691640269936	36.16994904283157	16.58724693568379
3	17.158104944222558	4.708029197080292	60.97507230409035	17.158793554606802
4	13.639994491116928	4.834733507781298	60.90483404489739	20.62043795620438
5	11.010880044071063	6.026029472524446	62.55336730477895	20.409723178625534
6	15.28783914061424	6.762153973281918	65.33741908827984	12.61258779782399
7	56.671946012945874	2.808153146949456	35.40352568516733	5.116375154937336
8	55.18317036220907	3.0553642748932655	34.71216085938576	7.049304503511913
9	50.1728412064454	4.99242528577331	37.722765459303126	7.111968048478171
10	25.55708580085388	17.983060184547583	41.93499517972731	14.52485883487123
11	17.379837487949317	15.309186062525823	46.603084974521416	20.707891475003443
12	15.849745214157828	12.64082082357802	52.669742459716296	18.839691502547858
13	18.8279851260157	14.178487811596199	53.53394849194326	13.45957857044484
14	15.535738878942295	16.784878115961988	52.31717394298306	15.362209062112656
15	11.021209199834733	15.184547582977551	55.024101363448565	18.770141853739155
16	11.548684754166093	15.05371160997108	51.34003580773998	22.05756782812285
17	10.933067070651425	15.386310425561216	49.45875223798375	24.22187026580361
18	12.558876187852913	14.161272551990084	53.07395675526787	20.205894504889134
19	12.72483129045586	17.174631593444428	52.663544966258094	17.436992149841622
20	12.401184409860901	21.02396364137171	50.196942569893956	16.377909378873433
21	11.767662856355873	21.575540559151634	50.909654317587105	15.747142266905383
22	9.860212091998347	19.695634210163888	48.68406555570858	21.760088142129185
23	10.282330257540284	20.041316623054676	46.30973695083322	23.36661616857182
24	11.920534361658174	20.032364688059495	45.95923426525272	22.08786668502961
25	11.50461368957444	21.601707753752926	43.668916127255194	23.224762429417435
26	11.288390028921636	21.61961162374329	45.460680347059636	21.631318000275442
27	12.567828122848093	20.072304090345682	47.737226277372265	19.622641509433965
28	10.532984437405316	17.99132350915852	48.57044484230823	22.905247211127943
29	13.8155901390993	16.223660652802643	46.6430243768076	23.317724831290455
30	18.281917091309737	17.16154799614378	44.11238121470872	20.444153697837763
31	16.80484781710508	21.782123674425012	41.417160170775375	19.99586833769453
32	16.54524170224487	20.292659413303955	40.24652251755956	22.915576366891614
33	16.99765872469357	16.25877978239912	40.244456686406835	26.499104806500483
34	17.892163613827297	14.882936234678418	42.30822200798788	24.916678143506406
35	21.21402010742322	15.467566450902078	38.6241564522793	24.6942569893954
36	21.030161134829914	16.269108938162788	39.00289216361383	23.697837763393473
37	16.517697286875084	17.190469632282053	44.92080980581187	21.371023275030986
38	16.799338934031123	17.111279438093927	45.15631455722352	20.933067070651425
39	17.80126704310701	14.555846302162237	43.49745214157829	24.14543451315246
40	21.003993940228618	14.223247486572097	42.10852499655695	22.66423357664234
41	22.482440435201763	15.59358215121884	39.46219528990497	22.461782123674425
42	22.974796859936646	14.909792039663957	42.99338934031125	19.122021760088142
43	18.276408208235782	14.002892163613827	47.16430243768076	20.55639719046963
44	17.28274342377083	14.338245420740945	42.734471835835286	25.644539319652942
45	20.824955240325025	12.310976449524858	40.08607629803058	26.777992012119544
46	22.682137446632694	13.626222283432035	43.206858559427076	20.484781710508194
47	16.634761052196666	17.642198044346507	46.92948629665336	18.79355460680347
48	16.075609420190055	16.880594959371987	47.04930450351191	19.994491116926046
49	20.408345957857044	12.458339071753203	47.251755956479826	19.88155901390993
50	19.60749208098058	11.094201900564661	42.902492769590964	26.395813248863792
51	17.474177110590826	11.72083735022724	39.41812422531331	31.386861313868614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	612.0
2	869.0
3	8684.5
4	16500.0
5	9744.0
6	2988.0
7	2776.0
8	2564.0
9	2536.5
10	2509.0
11	2377.0
12	2245.0
13	2068.0
14	1891.0
15	1743.0
16	1595.0
17	1483.5
18	1372.0
19	1258.5
20	1145.0
21	1062.0
22	979.0
23	901.5
24	824.0
25	777.5
26	745.5
27	760.0
28	792.5
29	825.0
30	828.5
31	832.0
32	910.5
33	989.0
34	1107.0
35	1225.0
36	1330.5
37	1436.0
38	1635.0
39	1834.0
40	2560.5
41	3287.0
42	4407.0
43	5527.0
44	6641.0
45	7755.0
46	10429.0
47	13103.0
48	16200.5
49	19298.0
50	19196.0
51	19094.0
52	15550.0
53	12006.0
54	9106.0
55	6206.0
56	4838.5
57	3471.0
58	2999.5
59	2528.0
60	2289.5
61	2051.0
62	1926.0
63	1801.0
64	1645.0
65	1489.0
66	1238.0
67	987.0
68	837.5
69	688.0
70	590.5
71	493.0
72	427.0
73	361.0
74	317.5
75	216.5
76	159.0
77	123.5
78	88.0
79	69.0
80	50.0
81	34.5
82	19.0
83	16.0
84	13.0
85	7.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	145220.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.77578845888996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23488733184588	41.289078639305885
2	11.15721350191151	11.776614791351053
3	4.504116595555904	7.13124913923702
4	2.1672472958338225	4.575127392921085
5	1.1469057032136847	3.0264426387549923
6	0.611943998643024	1.9377496212642886
7	0.4292741483018228	1.5858697149153012
8	0.28705262196474474	1.2119542762704862
9	0.21659425111885283	1.028783914061424
>10	1.131248287470153	10.882109902217325
>50	0.08089664800824624	2.9011155488224762
>100	0.023486123615297297	1.9274204655006197
>500	0.006523923226471471	2.159482164991048
>1k	0.0013047846452942941	0.692742046550062
>5k	0.0	0.0
>10k+	0.0013047846452942941	7.874259743836936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11435	7.874259743836936	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	1006	0.692742046550062	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	725	0.499242528577331	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	685	0.4716981132075472	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	590	0.4062801267043107	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	587	0.40421429555157695	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	549	0.3780471009502823	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	277	0.19074507643575264	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	253	0.17421842721388237	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	202	0.13909929761740808	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	172	0.11844098609007025	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	172	0.11844098609007025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	170	0.11706376532158104	No Hit
GTGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	160	0.11017766147913512	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	152	0.10466877840517835	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	148	0.10191433686819998	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	146	0.10053711609971079	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5309186062525822	0.0
2	0.0	0.0	0.0	1.9735573612450075	0.0
3	0.0	0.0	0.0	2.7874948354221183	0.0
4	0.0	0.0	0.0	3.950557774411238	0.0
5	0.0	0.0	0.0	6.4109626773171735	0.0
6	0.0	0.0	0.0	8.247486572097507	0.0
7	0.0	0.0	0.0	10.005508883073956	0.0
8	0.0	0.0	0.0	12.652527200110178	0.0
9	0.0	0.0	0.0	13.914061424046274	0.0
10	0.0	0.0	0.0	16.716705687921774	0.0
11	0.0	0.0	0.0	23.108387274480098	0.0
12	0.0	0.0	0.0	28.674425010329156	0.0
13	0.0	0.0	0.0	30.60391130698251	0.0
14	0.0	0.0	0.0	31.283569756231923	0.0
15	0.0	0.0	0.0	32.31855116375155	0.0
16	0.0	0.0	0.0	34.581324886379285	0.0
17	0.0	0.0	0.0	37.46591378597989	0.0
18	6.886103842445945E-4	0.0	0.0	40.124638479548274	0.0
19	6.886103842445945E-4	0.0	0.0	41.70706514254235	0.0
20	6.886103842445945E-4	0.0	0.0	43.10701005371161	0.0
21	6.886103842445945E-4	0.0	0.0	44.55653491254648	0.0
22	6.886103842445945E-4	0.0	0.0	45.827709681862004	0.0
23	0.001377220768489189	0.0	0.0	46.729789285222424	0.0
24	0.001377220768489189	0.0	0.0	47.20424183996695	0.0
25	0.001377220768489189	0.0	0.0	47.54372675939953	0.0
26	0.001377220768489189	0.0	0.0	47.81090758848644	0.0
27	0.001377220768489189	0.0	0.0	48.01817931414406	0.0
28	0.001377220768489189	0.0	0.0	48.23715741633384	0.0
29	0.001377220768489189	0.0	0.0	48.4203277785429	0.0
30	0.001377220768489189	0.0	0.0	48.626222283432035	0.0
31	0.001377220768489189	0.0	0.0	48.781848230271315	0.0
32	0.001377220768489189	0.0	0.0	48.92232474865721	0.0
33	0.001377220768489189	0.0	0.0	49.08414818895469	0.0
34	0.001377220768489189	0.0	0.0	49.22806775926181	0.0
35	0.001377220768489189	0.0	0.0	49.40159757609145	0.0
36	0.001377220768489189	0.0	0.0	49.53449938025065	0.0
37	0.001377220768489189	0.0	0.0	49.672221457099575	0.0
38	0.001377220768489189	0.0	0.0	49.843685442776476	0.0
39	0.001377220768489189	0.0	0.0	50.11499793416885	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAGG	20	7.0129195E-4	45.000004	2
ATGACGA	20	7.0129195E-4	45.000004	37
GGGTACT	20	7.0129195E-4	45.000004	7
CAACCCG	20	7.0129195E-4	45.000004	23
GGCCGAT	20	7.0129195E-4	45.000004	8
AGGGTCT	20	7.0129195E-4	45.000004	6
CGAAGGG	20	7.0129195E-4	45.000004	41
ACTCCGG	20	7.0129195E-4	45.000004	30
AACCCGA	20	7.0129195E-4	45.000004	24
TAGCATA	20	7.0129195E-4	45.000004	30
TCTATAG	20	7.0129195E-4	45.000004	1
GATGACG	20	7.0129195E-4	45.000004	36
GCAACCC	20	7.0129195E-4	45.000004	22
GCTATAG	20	7.0129195E-4	45.000004	9
CGGGTAC	20	7.0129195E-4	45.000004	6
TCGTTGG	20	7.0129195E-4	45.000004	2
TGCTATG	20	7.0129195E-4	45.000004	1
TGCTATA	20	7.0129195E-4	45.000004	8
GTAGGGT	20	7.0129195E-4	45.000004	4
GCACCGA	20	7.0129195E-4	45.000004	9
>>END_MODULE