##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145450
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848765898934342	33.0	31.0	34.0	30.0	34.0
2	32.06710897215538	33.0	31.0	34.0	30.0	34.0
3	32.154575455482984	33.0	31.0	34.0	30.0	34.0
4	35.76645582674458	37.0	35.0	37.0	35.0	37.0
5	35.858026813337915	37.0	35.0	37.0	35.0	37.0
6	35.85746992093503	37.0	35.0	37.0	35.0	37.0
7	35.833351667239604	37.0	35.0	37.0	35.0	37.0
8	35.67463733241664	37.0	35.0	37.0	33.0	37.0
9	37.48173942935717	39.0	37.0	39.0	34.0	39.0
10	37.0770849089034	39.0	37.0	39.0	33.0	39.0
11	37.10749398418701	39.0	37.0	39.0	33.0	39.0
12	37.11872121003781	39.0	37.0	39.0	33.0	39.0
13	37.04772774149192	39.0	37.0	39.0	33.0	39.0
14	38.05008594018563	40.0	37.0	41.0	33.0	41.0
15	38.093991062220695	40.0	37.0	41.0	33.0	41.0
16	38.26132004125129	40.0	38.0	41.0	33.0	41.0
17	38.30282571330354	40.0	38.0	41.0	33.0	41.0
18	37.98613956686147	39.0	37.0	40.0	34.0	41.0
19	37.58225507047095	38.0	37.0	40.0	34.0	41.0
20	36.963513234788586	38.0	35.0	40.0	33.0	41.0
21	36.86628394637332	38.0	35.0	40.0	33.0	41.0
22	36.87748367136473	38.0	35.0	40.0	33.0	41.0
23	36.77442420075627	38.0	35.0	40.0	33.0	41.0
24	36.657903059470605	38.0	35.0	40.0	33.0	41.0
25	36.560233757304914	38.0	35.0	40.0	33.0	41.0
26	36.58271570986593	38.0	35.0	40.0	33.0	41.0
27	36.53511172224132	38.0	35.0	40.0	33.0	41.0
28	36.48766586455827	38.0	35.0	40.0	33.0	41.0
29	36.32129941560674	38.0	35.0	40.0	32.0	41.0
30	36.04470952217257	38.0	35.0	40.0	31.0	41.0
31	35.64760398762461	37.0	35.0	40.0	30.0	41.0
32	35.3037194912341	38.0	35.0	40.0	27.0	41.0
33	34.66666895840495	38.0	35.0	40.0	22.0	41.0
34	34.1963492609144	38.0	34.0	40.0	18.0	41.0
35	33.84543141973187	38.0	34.0	40.0	16.0	41.0
36	33.54549329666552	38.0	33.0	40.0	15.0	41.0
37	33.41172911653489	37.0	33.0	40.0	15.0	41.0
38	33.28341010656583	37.0	33.0	40.0	12.0	41.0
39	33.258824338260574	37.0	33.0	40.0	12.0	41.0
40	33.101423169474046	37.0	33.0	40.0	10.0	41.0
41	32.92198693709178	37.0	33.0	40.0	10.0	41.0
42	32.648621519422484	37.0	32.0	40.0	10.0	41.0
43	32.49705053282915	37.0	31.0	40.0	10.0	41.0
44	32.39218288071502	37.0	31.0	40.0	10.0	41.0
45	32.37127535235476	36.0	31.0	40.0	10.0	41.0
46	32.07199037469921	36.0	31.0	40.0	10.0	41.0
47	31.94014437951186	36.0	31.0	40.0	10.0	41.0
48	31.843492609144036	35.0	31.0	40.0	10.0	41.0
49	31.867370230319697	35.0	31.0	40.0	9.0	41.0
50	31.695503609487798	35.0	30.0	40.0	8.0	41.0
51	30.77465108284634	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	7.0
13	4.0
14	4.0
15	13.0
16	20.0
17	26.0
18	56.0
19	116.0
20	201.0
21	327.0
22	530.0
23	877.0
24	1607.0
25	2980.0
26	4349.0
27	4879.0
28	4558.0
29	4269.0
30	4165.0
31	4361.0
32	5026.0
33	5947.0
34	8696.0
35	11912.0
36	15177.0
37	16038.0
38	27056.0
39	22234.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.89309040907528	4.385699553111035	37.765555173599175	36.9556548642145
2	42.84427638363699	4.668958404950155	35.713303540735645	16.773461670677207
3	17.430732210381574	4.777586799587487	60.651082846338944	17.14059814369199
4	14.140941904434515	4.822963217600551	60.46820213131661	20.567892746648333
5	11.26503953248539	6.099690615331729	62.07287727741492	20.562392574767962
6	15.406668958404952	6.800275008594019	65.40391887246476	12.389137160536267
7	56.763836369886555	2.809900309384668	35.31660364386387	5.109659676864902
8	55.155723616363005	3.060845651426607	34.655207975249226	7.128222756961156
9	50.168442763836374	5.0553454795462365	37.65761430044689	7.118597456170505
10	25.647988999656242	18.083877621175663	41.74424200756274	14.523891371605362
11	17.434857339291852	15.531797868683398	46.47576486765212	20.55757992437264
12	15.739429357167412	12.855964248882778	52.22550704709522	19.17909934685459
13	18.906153317291167	14.280508765898933	53.54898590580955	13.264352011000344
14	15.669989687177724	16.97009281540048	51.94774836713647	15.412169130285323
15	11.183911997249913	15.408044001375043	54.690271570986596	18.71777243038845
16	11.612925403918872	15.107597112409762	51.24647645238913	22.03300103128223
17	10.979718116191131	15.49467170849089	49.31179099346855	24.213819181849434
18	12.761773805431421	14.259195599862496	52.84358886215195	20.135441732554142
19	12.950154692334134	17.277414919216223	52.471639738741835	17.300790649707803
20	12.697146785837058	20.78308697146786	50.00481265039532	16.514953592299758
21	11.95393606050189	21.546235819869374	50.620144379511856	15.879683740116878
22	10.049501546923342	19.55379855620488	48.643520110003436	21.75317978686834
23	10.609144035751116	20.114816088002748	46.14025438294947	23.135785493296666
24	12.09969061533173	19.892059126847712	46.17394293571674	21.834307322103815
25	11.67480233757305	21.527672739773116	43.61361292540391	23.183911997249915
26	11.434169817806806	21.711928497765555	45.357167411481605	21.496734272946032
27	12.863526985218288	19.916122378824337	47.70711584737023	19.513234788587145
28	10.739085596424887	18.167755242351326	48.36232382261946	22.730835338604333
29	13.903059470608456	16.147129597799932	46.631832244757646	23.31797868683396
30	18.371949123410104	17.2059126847714	44.05843932622894	20.363698865589548
31	16.854589205912685	21.592987280852526	41.43623238226194	20.116191130972844
32	16.595393606050187	20.49570299071846	40.34101065658302	22.567892746648333
33	16.971467858370573	16.406325197662426	40.16844276383637	26.45376418013063
34	17.951185974561707	15.14472327260227	42.15950498453076	24.74458576830526
35	21.21553798556205	15.502921966311447	38.60433138535579	24.67720866277071
36	21.2251632863527	16.40907528360261	38.83533860433139	23.530422825713305
37	16.578893090409075	17.168786524578895	45.00171880371261	21.250601581299417
38	17.08353386043314	17.224475764867652	44.709522172567894	20.982468202131315
39	17.85080783774493	14.739773117909936	43.4534204193881	23.955998624957033
40	20.871777243038846	14.376074252320384	42.222756961155035	22.529391543485733
41	22.381574424200757	15.74767961498797	39.333104159504984	22.53764180130629
42	22.952217256789275	14.906840838776212	42.85733929185287	19.283602612581642
43	18.281883808869026	13.919559986249569	47.15297353042283	20.645582674458577
44	17.22103815744242	14.413887933997938	42.792024750773464	25.573049157786183
45	20.84977655551736	12.426263320728772	40.103815744242006	26.620144379511864
46	22.81608800275009	13.75524235132348	42.89240288759024	20.536266758336197
47	16.686146442076318	17.684427638363697	46.86008937779306	18.76933654176693
48	15.938123066345824	16.808525266414577	47.2059126847714	20.0474389824682
49	20.26400825025782	12.730147817119287	47.07184599518735	19.933997937435542
50	19.559298728085253	11.269164661395669	42.95634238569955	26.215194224819527
51	17.74905465795806	11.931247851495359	39.24372636644895	31.075971124097627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	277.0
1	570.5
2	864.0
3	8582.5
4	16301.0
5	9756.0
6	3211.0
7	2935.5
8	2660.0
9	2582.5
10	2505.0
11	2332.5
12	2160.0
13	2037.5
14	1915.0
15	1749.5
16	1584.0
17	1473.5
18	1363.0
19	1239.0
20	1115.0
21	1016.0
22	917.0
23	844.0
24	771.0
25	780.0
26	779.5
27	770.0
28	784.0
29	798.0
30	864.0
31	930.0
32	953.0
33	976.0
34	1125.5
35	1275.0
36	1380.5
37	1486.0
38	1686.0
39	1886.0
40	2650.0
41	3414.0
42	4500.0
43	5586.0
44	6638.5
45	7691.0
46	10318.0
47	12945.0
48	16075.5
49	19206.0
50	19141.5
51	19077.0
52	15580.5
53	12084.0
54	9087.5
55	6091.0
56	4821.5
57	3552.0
58	3053.5
59	2555.0
60	2354.0
61	2153.0
62	1960.5
63	1768.0
64	1638.0
65	1508.0
66	1257.0
67	1006.0
68	859.5
69	713.0
70	592.5
71	472.0
72	439.0
73	406.0
74	360.5
75	244.0
76	173.0
77	134.5
78	96.0
79	77.0
80	58.0
81	38.0
82	18.0
83	13.0
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	145450.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.09178411825369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29375048561292	41.56754898590581
2	11.082334049882157	11.767617738054314
3	4.536272046826034	7.225163286352698
4	2.207920022791433	4.6888965280165005
5	1.1356867214006372	3.0147817119284976
6	0.616404651524177	1.9635613612925404
7	0.4221594882287431	1.5689240288759023
8	0.28230297065603066	1.199037469920935
9	0.20719484084846285	0.9900309384668271
>10	1.0981326564968532	10.518391199724991
>50	0.08158296858408225	2.7679614987968377
>100	0.02719432286136075	2.1175661739429357
>500	0.006474838776514464	2.1533172911653486
>1k	0.0012949677553028929	0.730147817119285
>5k	0.0	0.0
>10k+	0.0012949677553028929	7.727053970436576
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11239	7.727053970436576	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	1062	0.730147817119285	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	708	0.48676521141285667	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	690	0.47438982468202134	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	592	0.40701271914747333	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	575	0.3953248539016845	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	567	0.38982468202131315	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	266	0.18288071502234446	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	0.14644207631488484	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	202	0.13887933997937435	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	170	0.11687865245788931	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	167	0.11481608800275007	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	164	0.11275352354761087	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	163	0.11206600206256445	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	153	0.10519078721210039	No Hit
GTGCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	152	0.10450326572705396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5053282915091096	0.0
2	0.0	0.0	0.0	1.8968717772430388	0.0
3	0.0	0.0	0.0	2.7459608112753524	0.0
4	0.0	0.0	0.0	3.920935029219663	0.0
5	0.0	0.0	0.0	6.369886558954967	0.0
6	0.0	0.0	0.0	8.15125472671021	0.0
7	0.0	0.0	0.0	9.817806806462702	0.0
8	0.0	0.0	0.0	12.511515984874528	0.0
9	0.0	0.0	0.0	13.746992093502922	0.0
10	0.0	0.0	0.0	16.46888965280165	0.0
11	0.0	0.0	0.0	22.69989687177724	0.0
12	0.0	0.0	0.0	28.155379855620488	0.0
13	0.0	0.0	0.0	30.067377105534547	0.0
14	0.0	0.0	0.0	30.75421106909591	0.0
15	0.0	0.0	0.0	31.74561705053283	0.0
16	0.0	0.0	0.0	33.99312478514953	0.0
17	0.0	0.0	0.0	36.94396699896872	0.0
18	0.0	0.0	0.0	39.54898590580956	0.0
19	0.0	0.0	0.0	41.09247163973874	0.0
20	0.0	0.0	0.0	42.42351323478859	0.0
21	0.0	0.0	0.0	43.93606050189069	0.0
22	0.0	0.0	0.0	45.17153661051908	0.0
23	0.0	0.0	0.0	46.05706428325885	0.0
24	0.0	0.0	0.0	46.56514266070815	0.0
25	0.0	0.0	0.0	46.875902371949124	0.0
26	0.0	0.0	0.0	47.13303540735648	0.0
27	0.0	0.0	0.0	47.35510484702647	0.0
28	0.0	0.0	0.0	47.52698521828807	0.0
29	0.0	0.0	0.0	47.718116191130974	0.0
30	0.0	0.0	0.0	47.9243726366449	0.0
31	0.0	0.0	0.0	48.08731522860089	0.0
32	0.0	0.0	0.0	48.228944654520454	0.0
33	0.0	0.0	0.0	48.382261945685805	0.0
34	0.0	0.0	0.0	48.52457889309041	0.0
35	0.0	0.0	0.0	48.7081471295978	0.0
36	0.0	0.0	0.0	48.84427638363699	0.0
37	0.0	0.0	0.0	48.99828119628739	0.0
38	0.0	0.0	0.0	49.16878652457889	0.0
39	0.0	0.0	0.0	49.43348229632176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGACC	30	2.152401E-6	45.000004	6
TGTTTCG	20	7.012962E-4	45.0	1
GGTAATG	20	7.012962E-4	45.0	8
GTATTGG	20	7.012962E-4	45.0	2
TATGGGT	25	3.8739672E-5	45.0	4
ATGGAAG	20	7.012962E-4	45.0	40
GGGTACA	20	7.012962E-4	45.0	7
CGTTAGG	20	7.012962E-4	45.0	2
GTGCTAG	20	7.012962E-4	45.0	1
GTACTTG	25	3.8739672E-5	45.0	1
AAACACG	40	6.7448127E-9	45.0	40
ATTGGGC	20	7.012962E-4	45.0	4
GAGTTGC	20	7.012962E-4	45.0	10
TTACGGG	20	7.012962E-4	45.0	3
GGCATAG	20	7.012962E-4	45.0	7
AACACGT	40	6.7448127E-9	45.0	41
CGTTTTT	3555	0.0	44.55696	1
TGGGAGA	75	0.0	42.0	6
TTGGGAG	170	0.0	41.029415	5
CTCGGGA	55	6.002665E-11	40.909092	4
>>END_MODULE