FastQCFastQC Report
Sat 14 Jan 2017
SRR2933576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104978
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1205511.48335841795424No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC11281.0745108498923583No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC6440.6134618682009564No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT4970.47343252872030334TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC4770.454380917906609TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC4390.41818285736058985TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC3290.3133989978852712TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG1900.1809903027300958No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1830.17432223894530283No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1640.15622320867229325No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.15146030596886967No Hit
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG1590.15146030596886967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.1486025643468155No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1330.12669321191106708No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT1270.1209777286669588No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT1170.11145192326011163No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA1160.11049934271942692No Hit
GTTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC1150.10954676217874221No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTT1050.10002095677189506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG302.146633E-645.0000042
TTGGGTG351.1978773E-745.0000045
TACTGGG302.146633E-645.0000043
GCTCTTG351.1978773E-745.0000041
TTATGGG406.71389E-945.03
TAGCGGG207.0039934E-445.03
ACTAATC253.8665454E-545.010
TTCGTTG207.0039934E-445.01
CTCCTTG207.0039934E-445.01
GCGATGG253.8665454E-545.09
AATTCGA253.8665454E-545.013
GGACTAA253.8665454E-545.08
CTCTCGG207.0039934E-445.02
TTCGAGC253.8665454E-545.015
GGCGATG502.1827873E-1145.08
GGATCAA207.0039934E-445.08
TCCGGGA207.0039934E-445.04
CGGGAGG207.0039934E-445.06
ATTCGAG253.8665454E-545.014
CGGGAAT253.8665454E-545.06
Produced by FastQC (version 0.11.5)