##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104978
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03746499266513	33.0	31.0	34.0	30.0	34.0
2	32.28974642306007	34.0	31.0	34.0	30.0	34.0
3	32.26487454514279	33.0	31.0	34.0	30.0	34.0
4	35.93706300367696	37.0	35.0	37.0	35.0	37.0
5	35.98766408199813	37.0	35.0	37.0	35.0	37.0
6	35.98867381737126	37.0	35.0	37.0	35.0	37.0
7	36.045781020785306	37.0	35.0	37.0	35.0	37.0
8	35.842728952732955	37.0	35.0	37.0	35.0	37.0
9	37.68179047038427	39.0	38.0	39.0	35.0	39.0
10	37.267075006191774	39.0	37.0	39.0	34.0	39.0
11	37.28429766236735	39.0	37.0	39.0	34.0	39.0
12	37.32761149955229	39.0	37.0	39.0	34.0	39.0
13	37.239031035074014	39.0	37.0	39.0	34.0	39.0
14	38.39648307264379	40.0	38.0	41.0	33.0	41.0
15	38.432061955838364	40.0	38.0	41.0	33.0	41.0
16	38.52574825201471	40.0	38.0	41.0	34.0	41.0
17	38.51177389548286	40.0	38.0	41.0	34.0	41.0
18	37.84957800682048	39.0	38.0	40.0	34.0	41.0
19	37.16576806568995	37.0	37.0	40.0	34.0	41.0
20	36.30841700165749	35.0	35.0	40.0	33.0	41.0
21	36.18049496084894	35.0	35.0	40.0	33.0	41.0
22	36.18523881194155	35.0	35.0	40.0	33.0	41.0
23	36.14662119682219	35.0	35.0	40.0	33.0	41.0
24	35.967259806816664	35.0	35.0	40.0	33.0	41.0
25	35.85154032273429	35.0	35.0	40.0	32.0	41.0
26	35.82147688087028	35.0	35.0	40.0	32.0	41.0
27	35.78819371677876	35.0	35.0	40.0	32.0	41.0
28	35.63241822096058	35.0	35.0	40.0	32.0	41.0
29	35.46876488407095	35.0	35.0	40.0	31.0	41.0
30	35.190868562937	35.0	35.0	40.0	30.0	41.0
31	34.59300996399246	35.0	35.0	40.0	25.0	41.0
32	33.75004286612433	35.0	33.0	40.0	21.0	41.0
33	32.7948046257311	35.0	33.0	40.0	15.0	41.0
34	31.970889138676675	35.0	31.0	40.0	10.0	41.0
35	31.343786317133112	35.0	30.0	40.0	8.0	41.0
36	31.061412867457943	35.0	27.0	40.0	7.0	41.0
37	30.778896530701672	35.0	25.0	40.0	7.0	41.0
38	30.57253900817314	35.0	24.0	40.0	7.0	41.0
39	30.464306807140545	35.0	23.0	40.0	7.0	41.0
40	30.360370744346433	35.0	23.0	40.0	7.0	41.0
41	30.219855588790033	35.0	23.0	40.0	7.0	41.0
42	30.00127645792452	35.0	22.0	40.0	7.0	41.0
43	29.676551277410503	35.0	21.0	39.0	7.0	41.0
44	29.430423517308387	35.0	21.0	39.0	7.0	41.0
45	29.412648364419212	35.0	20.0	39.0	7.0	41.0
46	29.181418963973403	35.0	20.0	39.0	7.0	41.0
47	28.983034540570404	35.0	20.0	39.0	7.0	41.0
48	28.885480767398885	35.0	20.0	39.0	7.0	41.0
49	28.88621425441521	35.0	20.0	39.0	7.0	41.0
50	28.74976661776753	35.0	19.0	39.0	7.0	40.0
51	27.833965211758652	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	1.0
12	0.0
13	3.0
14	3.0
15	2.0
16	10.0
17	20.0
18	63.0
19	130.0
20	219.0
21	386.0
22	624.0
23	977.0
24	1826.0
25	3608.0
26	5283.0
27	5444.0
28	4582.0
29	3908.0
30	3743.0
31	3690.0
32	3788.0
33	4179.0
34	5675.0
35	9177.0
36	10991.0
37	9843.0
38	16097.0
39	10695.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.749014079140391	3.7083960448855953	30.81693307169121	49.725656804282806
2	53.37689801672732	3.6064699270323306	29.911981558040736	13.104650498199621
3	12.653127321915067	4.346625007144354	70.46905065823316	12.531197012707423
4	10.603173998361562	3.771266360570786	70.1299319857494	15.495627655318255
5	8.4008077882985	4.9505610699384635	71.24540379889119	15.403227342871839
6	11.523366800662997	5.469717464611633	73.33631808569415	9.670597649031226
7	44.643639619729846	2.1756939549238887	48.90453237821258	4.276134047133685
8	42.4965230810265	2.7615309874449885	48.62066337708853	6.121282554439978
9	39.272037950808745	4.251366953075882	50.83922345634323	5.637371639772143
10	19.836537179218503	14.874545142791822	54.66288174665168	10.626035931337995
11	13.356131760940388	13.123702109013315	57.358684676789416	16.161481453256872
12	12.217798014822154	10.504105622130352	62.042523195336166	15.23557316771133
13	14.581150336260931	12.678846996513554	62.69599344624588	10.044009220979634
14	12.014898359656309	14.667835165463238	60.69176398864524	12.62550248623521
15	8.116938787174456	13.240869515517536	63.973403951304086	14.668787746003925
16	7.864504943893007	13.177999199832346	61.50717293147135	17.45032292480329
17	7.860694621730267	13.117986625769209	59.06094610299301	19.960372649507516
18	8.692297433748024	12.296862199698985	62.82649698031969	16.184343386233305
19	9.483891863057021	14.080092971860772	62.692183124083144	13.743832040999065
20	9.747756672826688	17.24170778639334	60.27548629236602	12.735049248413954
21	8.838042256472784	18.061879631922878	60.723199146487836	12.3768789651165
22	7.194840823791651	17.247423269637448	58.16647297529006	17.391262931280842
23	7.537769818438149	17.100725866372002	56.86334279563338	18.498161519556476
24	9.219074472746671	16.251024024081236	56.35371220636705	18.176189296805045
25	8.127417173121987	17.786583855664997	54.759092381260835	19.32690658995218
26	8.176951361237593	18.313360894663646	56.7185505534493	16.79113719064947
27	9.551525081445636	16.95498104364724	58.30650231477071	15.18699156013641
28	7.653984644401684	15.137457372020805	59.059993522452324	18.14856446112519
29	10.519346910781307	14.24774714702128	57.21293985406466	18.01996608813275
30	14.143915868086646	14.988854807673988	55.08106460401226	15.786164720227095
31	12.322581874297473	19.796528796509744	51.02973956448018	16.851149764712606
32	12.283526072129398	20.27567680847416	49.063613328506925	18.37718379088952
33	14.214406828097314	16.889252986339994	47.934805387795535	20.96153479776715
34	14.19916553944636	16.28817466516794	47.448989312046336	22.06367048333937
35	16.54537141115281	17.596067747528053	43.9863590466574	21.872201794661738
36	17.545580978871765	17.615119358341747	44.9456076511269	19.893692011659585
37	13.562841738268972	19.468841090514204	47.59854445693384	19.36977271428299
38	12.491188629998666	22.981005544018746	47.78334508182667	16.744460744155916
39	15.773781173198195	19.72127493379565	47.395644801767986	17.109299091238164
40	15.613747642363162	16.28817466516794	47.552820590980964	20.54525710148793
41	15.443235725580598	16.41963077978243	45.216140524681364	22.92099296995561
42	20.117548438720494	15.872849549429406	46.0363123702109	17.9732896416392
43	18.644858922821925	16.638724304139917	47.03747451847053	17.678942254567623
44	15.14698317742765	17.31600906856675	45.59526757987388	21.941740174131724
45	17.201699403684582	15.55849797100345	44.27118062832213	22.968621996989846
46	17.952332869744136	15.77282859265751	48.018632475375796	18.25620606222256
47	13.11989178685058	18.5838937682181	50.35150221951265	17.94471222541866
48	13.460915620415706	16.939739754996285	50.96305892663224	18.636285697955763
49	18.14951704166587	13.464725942578445	50.72681895254244	17.658938063213245
50	16.277696279220407	12.086341900207662	47.86431442778487	23.77164739278706
51	14.175351025929242	12.073005772638076	44.79795766732077	28.953685534111912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	264.0
1	438.0
2	612.0
3	9456.5
4	18301.0
5	10950.0
6	3599.0
7	3317.0
8	3035.0
9	2949.5
10	2864.0
11	2811.5
12	2759.0
13	2561.5
14	2364.0
15	2166.0
16	1968.0
17	1793.5
18	1619.0
19	1502.5
20	1386.0
21	1224.0
22	1062.0
23	1000.5
24	939.0
25	864.0
26	732.5
27	676.0
28	671.0
29	666.0
30	674.0
31	682.0
32	723.5
33	765.0
34	781.5
35	798.0
36	957.5
37	1117.0
38	1237.5
39	1358.0
40	2011.0
41	2664.0
42	3551.0
43	4438.0
44	4825.0
45	5212.0
46	6943.0
47	8674.0
48	10386.5
49	12099.0
50	11250.0
51	10401.0
52	8243.0
53	6085.0
54	4459.5
55	2834.0
56	2129.0
57	1424.0
58	1158.5
59	893.0
60	863.5
61	834.0
62	705.0
63	576.0
64	509.5
65	443.0
66	364.0
67	285.0
68	230.5
69	176.0
70	142.0
71	108.0
72	90.0
73	72.0
74	72.0
75	48.0
76	24.0
77	23.0
78	22.0
79	15.0
80	8.0
81	7.5
82	7.0
83	5.5
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	104978.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.12160643182381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19104830900548	43.129989140581834
2	9.737115805329411	10.345024671836004
3	3.799806333608292	6.055554497132733
4	1.8308646845748306	3.8903389281563756
5	0.9503998852347308	2.5243384328144947
6	0.6025176630922068	1.9204023700203854
7	0.36760750277947135	1.3669530758825659
8	0.20980525768389344	0.8916153860808931
9	0.17573431840189363	0.8401760368839186
>10	1.032887422443783	10.068776315037436
>50	0.06455546390273643	2.298576844672217
>100	0.03227773195136822	3.4969231648535883
>500	0.0017932073306315675	0.6134618682009564
>1k	0.0017932073306315675	1.0745108498923583
>5k	0.0	0.0
>10k+	0.0017932073306315675	11.48335841795424
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12055	11.48335841795424	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	1128	1.0745108498923583	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	644	0.6134618682009564	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	497	0.47343252872030334	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	477	0.454380917906609	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	439	0.41818285736058985	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	329	0.3133989978852712	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	190	0.1809903027300958	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	183	0.17432223894530283	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	164	0.15622320867229325	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.15146030596886967	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	159	0.15146030596886967	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	156	0.1486025643468155	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	133	0.12669321191106708	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	127	0.1209777286669588	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	117	0.11145192326011163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	116	0.11049934271942692	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	115	0.10954676217874221	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTT	105	0.10002095677189506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5105831698070071	0.0
2	0.0	0.0	0.0	1.841338185143554	0.0
3	0.0	0.0	0.0	2.5776829430928383	0.0
4	0.0	0.0	0.0	3.517879936748652	0.0
5	0.0	0.0	0.0	5.639276800853512	0.0
6	0.0	0.0	0.0	7.054811484310998	0.0
7	0.0	0.0	0.0	8.50178132561108	0.0
8	0.0	0.0	0.0	10.833698489207263	0.0
9	0.0	0.0	0.0	11.773895482863075	0.0
10	0.0	0.0	0.0	14.17916134809198	0.0
11	0.0	0.0	0.0	19.920364266798757	0.0
12	0.0	0.0	0.0	24.77757244375012	0.0
13	0.0	0.0	0.0	26.39410162129208	0.0
14	0.0	0.0	0.0	26.935167368400997	0.0
15	0.0	0.0	0.0	27.762959858256014	0.0
16	0.0	0.0	0.0	29.621444493131893	0.0
17	0.0	0.0	0.0	31.892396502124253	0.0
18	0.0	0.0	0.0	34.12524528948923	0.0
19	0.0	0.0	0.0	35.4293280496866	0.0
20	0.0	0.0	0.0	36.658156947169886	0.0
21	0.0	0.0	0.0	37.97462325439616	0.0
22	0.0	0.0	0.0	39.09295280916002	0.0
23	0.0	0.0	0.0	39.913124654689554	0.0
24	0.0	0.0	0.0	40.322734287183984	0.0
25	0.0	0.0	0.0	40.616129093714875	0.0
26	0.0	0.0	0.0	40.870468098077694	0.0
27	0.0	0.0	0.0	41.09813484730134	0.0
28	0.0	0.0	0.0	41.28198289165349	0.0
29	0.0	0.0	0.0	41.46106803330222	0.0
30	0.0	0.0	0.0	41.661109946846004	0.0
31	0.0	0.0	0.0	41.82685896092514	0.0
32	0.0	0.0	0.0	41.95259959229553	0.0
33	0.0	0.0	0.0	42.0973918344796	0.0
34	0.0	0.0	0.0	42.234563432338206	0.0
35	0.0	0.0	0.0	42.388881479929125	0.0
36	0.0	0.0	0.0	42.4965230810265	0.0
37	0.0	0.0	0.0	42.632742098344416	0.0
38	0.0	0.0	0.0	42.78515498485397	0.0
39	0.0	0.0	0.0	42.92613690487531	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGG	30	2.146633E-6	45.000004	2
TTGGGTG	35	1.1978773E-7	45.000004	5
TACTGGG	30	2.146633E-6	45.000004	3
GCTCTTG	35	1.1978773E-7	45.000004	1
TTATGGG	40	6.71389E-9	45.0	3
TAGCGGG	20	7.0039934E-4	45.0	3
ACTAATC	25	3.8665454E-5	45.0	10
TTCGTTG	20	7.0039934E-4	45.0	1
CTCCTTG	20	7.0039934E-4	45.0	1
GCGATGG	25	3.8665454E-5	45.0	9
AATTCGA	25	3.8665454E-5	45.0	13
GGACTAA	25	3.8665454E-5	45.0	8
CTCTCGG	20	7.0039934E-4	45.0	2
TTCGAGC	25	3.8665454E-5	45.0	15
GGCGATG	50	2.1827873E-11	45.0	8
GGATCAA	20	7.0039934E-4	45.0	8
TCCGGGA	20	7.0039934E-4	45.0	4
CGGGAGG	20	7.0039934E-4	45.0	6
ATTCGAG	25	3.8665454E-5	45.0	14
CGGGAAT	25	3.8665454E-5	45.0	6
>>END_MODULE