SRR2933575.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105428
Sequences flagged as poor quality	0
Sequence length	51
%GC	32

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11900	11.28732405053686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	1114	1.0566452934704254	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	640	0.607049360701142	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	519	0.49227909094358235	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	516	0.48943354706529574	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	438	0.41544940622984405	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	287	0.2722236976894184	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	209	0.1982395568539667	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	192	0.18211480821034262	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	161	0.15271085480138105	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	148	0.1403801646621391	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.1346890769055659	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.1299465037750882	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	136	0.12899798914899266	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTTT	127	0.12046135751413287	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	124	0.11761581363584625	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	116	0.11002769662708199	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATG	113	0.10718215274879539	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	113	0.10718215274879539	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	111	0.10528512349660432	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCTG	20	7.00413E-4	45.0	1
GGGCACA	20	7.00413E-4	45.0	7
GGACCGA	25	3.8666585E-5	45.0	8
CTCTCGG	20	7.00413E-4	45.0	2
TGTTCGG	20	7.00413E-4	45.0	2
TGCTTTG	25	3.8666585E-5	45.0	1
CGGGATT	20	7.00413E-4	45.0	6
CGGGAGA	25	3.8666585E-5	45.0	6
GACCGAT	25	3.8666585E-5	45.0	9
CGGGACC	25	3.8666585E-5	45.0	6
CGGGAAT	20	7.00413E-4	45.0	6
TGTAGGG	25	3.8666585E-5	45.0	3
GGCCACC	25	3.8666585E-5	45.0	8
GATTTGT	20	7.00413E-4	45.0	10
GGGACCG	25	3.8666585E-5	45.0	7
TGGGCTT	25	3.8666585E-5	45.0	6
TGGGTGT	35	1.1979319E-7	45.0	6
TGGGCAC	25	3.8666585E-5	45.0	6
CCGGGAT	20	7.00413E-4	45.0	5
GGGAAGT	20	7.00413E-4	45.0	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content